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- PDB-1k0o: Crystal structure of a soluble form of CLIC1. An intracellular ch... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1k0o | ||||||
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Title | Crystal structure of a soluble form of CLIC1. An intracellular chloride ion channel | ||||||
![]() | CHLORIDE INTRACELLULAR CHANNEL PROTEIN 1 | ||||||
![]() | METAL TRANSPORT / Glutathione-S-Transferase superfamily / CLIC1 / NCC27 / Chloride ion channel | ||||||
Function / homology | ![]() chloride transport / chloride channel activity / brush border / chloride channel complex / positive regulation of osteoblast differentiation / regulation of mitochondrial membrane potential / platelet aggregation / nuclear envelope / nuclear membrane / vesicle ...chloride transport / chloride channel activity / brush border / chloride channel complex / positive regulation of osteoblast differentiation / regulation of mitochondrial membrane potential / platelet aggregation / nuclear envelope / nuclear membrane / vesicle / blood microparticle / cadherin binding / perinuclear region of cytoplasm / endoplasmic reticulum / signal transduction / mitochondrion / extracellular space / extracellular exosome / membrane / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Harrop, S.J. / DeMaere, M.Z. / Fairlie, W.D. / Reztsova, T. / Valenzuela, S.M. / Mazzanti, M. / Tonini, R. / Qiu, M.R. / Jankova, L. / Warton, K. ...Harrop, S.J. / DeMaere, M.Z. / Fairlie, W.D. / Reztsova, T. / Valenzuela, S.M. / Mazzanti, M. / Tonini, R. / Qiu, M.R. / Jankova, L. / Warton, K. / Bauskin, A.R. / Wu, W.M. / Pankhurst, S. / Campbell, T.J. / Breit, S.N. / Curmi, P.M.G. | ||||||
![]() | ![]() Title: Crystal structure of a soluble form of the intracellular chloride ion channel CLIC1 (NCC27) at 1.4-A resolution. Authors: Harrop, S.J. / DeMaere, M.Z. / Fairlie, W.D. / Reztsova, T. / Valenzuela, S.M. / Mazzanti, M. / Tonini, R. / Qiu, M.R. / Jankova, L. / Warton, K. / Bauskin, A.R. / Wu, W.M. / Pankhurst, S. / ...Authors: Harrop, S.J. / DeMaere, M.Z. / Fairlie, W.D. / Reztsova, T. / Valenzuela, S.M. / Mazzanti, M. / Tonini, R. / Qiu, M.R. / Jankova, L. / Warton, K. / Bauskin, A.R. / Wu, W.M. / Pankhurst, S. / Campbell, T.J. / Breit, S.N. / Curmi, P.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 96.6 KB | Display | ![]() |
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PDB format | ![]() | 74.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 375.2 KB | Display | ![]() |
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Full document | ![]() | 384.1 KB | Display | |
Data in XML | ![]() | 10.6 KB | Display | |
Data in CIF | ![]() | 16.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26881.605 Da / Num. of mol.: 2 / Fragment: CLIC1 / Mutation: E151G Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.09 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 5 Details: PEG MME 5K, ammonium sulphate, sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Dec 18, 2000 / Details: monochromator |
Radiation | Monochromator: yes / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→80 Å / Num. all: 47410 / Num. obs: 47410 / % possible obs: 97.96 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.2 % / Biso Wilson estimate: 30.1 Å2 / Rmerge(I) obs: 0.044 / Rsym value: 0.044 / Net I/σ(I): 9.7 |
Reflection shell | Resolution: 1.75→1.84 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.46 / Mean I/σ(I) obs: 1.8 / Num. unique all: 6668 / Rsym value: 0.46 / % possible all: 94 |
Reflection | *PLUS Lowest resolution: 80 Å / Num. obs: 48235 / % possible obs: 98.2 % / Num. measured all: 212823 |
Reflection shell | *PLUS % possible obs: 97 % / Mean I/σ(I) obs: 1.6 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 15.042 Å2
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Refinement step | Cycle: LAST / Resolution: 1.75→87.71 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Version: 5 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS σ(F): 0 / % reflection Rfree: 5.1 % / Rfactor obs: 0.243 / Rfactor Rfree: 0.272 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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