+
Open data
-
Basic information
Entry | Database: PDB / ID: 1jej | ||||||
---|---|---|---|---|---|---|---|
Title | T4 phage apo BGT | ||||||
![]() | DNA BETA-GLUCOSYLTRANSFERASE | ||||||
![]() | TRANSFERASE / Glycosyltransferase | ||||||
Function / homology | ![]() DNA beta-glucosyltransferase / DNA beta-glucosyltransferase activity / symbiont-mediated evasion of host restriction-modification system / DNA modification / virus-mediated perturbation of host defense response Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Morera, S. / Lariviere, L. / Kurzeck, J. / Aschke-Sonnenborn, U. / Freemont, P.S. / Janin, J. / Ruger, W. | ||||||
![]() | ![]() Title: High resolution crystal structures of T4 phage beta-glucosyltransferase: induced fit and effect of substrate and metal binding. Authors: Morera, S. / Lariviere, L. / Kurzeck, J. / Aschke-Sonnenborn, U. / Freemont, P.S. / Janin, J. / Ruger, W. #1: ![]() Title: T4 Phage beta-Glucosyltransferase: Substrate Binding and Proposed Catalytic Mechanism | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 85.7 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 65 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 422.8 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 429.5 KB | Display | |
Data in XML | ![]() | 16.5 KB | Display | |
Data in CIF | ![]() | 23.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1jg6C ![]() 1jg7C ![]() 1jiuC ![]() 1jivC ![]() 1jixC ![]() 1qkjS C: citing same article ( S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 40719.879 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.52 % | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 10000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K | ||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: May 9, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→20 Å / Num. all: 14640 / Num. obs: 14639 / % possible obs: 100 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 1 / Redundancy: 5.1 % / Biso Wilson estimate: 49 Å2 / Rmerge(I) obs: 0.044 / Net I/σ(I): 16.5 |
Reflection shell | Resolution: 2.5→2.59 Å / Redundancy: 5.1 % / Rmerge(I) obs: 0.223 / % possible all: 100 |
Reflection | *PLUS % possible obs: 100 % / Num. measured all: 74099 |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1QKJ Resolution: 2.5→20 Å / σ(F): 2 / σ(I): 0 / Stereochemistry target values: Engh & huber
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→20 Å
| |||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||
Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 20 Å / σ(F): 2 / Rfactor obs: 0.197 / % reflection Rfree: 5 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |