+Open data
-Basic information
Entry | Database: PDB / ID: 1jix | ||||||
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Title | T4 Phage BGT in Complex with Ca2+ | ||||||
Components | DNA BETA-GLUCOSYLTRANSFERASE | ||||||
Keywords | TRANSFERASE / Glycosyltransferase | ||||||
Function / homology | Function and homology information DNA beta-glucosyltransferase / DNA beta-glucosyltransferase activity / symbiont-mediated evasion of host restriction-modification system / DNA modification Similarity search - Function | ||||||
Biological species | Enterobacteria phage T4 (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.65 Å | ||||||
Authors | Morera, S. / Lariviere, L. / Kurzeck, J. / Aschke-Sonnenborn, U. / Freemont, P.S. / Janin, J. / Ruger, W. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2001 Title: High resolution crystal structures of T4 phage beta-glucosyltransferase: induced fit and effect of substrate and metal binding. Authors: Morera, S. / Lariviere, L. / Kurzeck, J. / Aschke-Sonnenborn, U. / Freemont, P.S. / Janin, J. / Ruger, W. #1: Journal: J.Mol.Biol. / Year: 1999 Title: T4 phage beta-glucosyltransferase : substrate binding and proposed catalytic mechanism Authors: Morera, S. / Imberty, A. / Aschke-Sonnenborn, U. / Ruger, W. / Freemont, P.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1jix.cif.gz | 96.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1jix.ent.gz | 70.8 KB | Display | PDB format |
PDBx/mmJSON format | 1jix.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ji/1jix ftp://data.pdbj.org/pub/pdb/validation_reports/ji/1jix | HTTPS FTP |
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-Related structure data
Related structure data | 1jejC 1jg6C 1jg7C 1jiuC 1jivC 1qkjS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 40719.879 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage T4 (virus) / Genus: T4-like viruses / Species: Enterobacteria phage T4 sensu lato / Production host: Escherichia coli (E. coli) / References: UniProt: P04547, DNA beta-glucosyltransferase |
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#2: Chemical | ChemComp-CA / |
#3: Chemical | ChemComp-UDP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.82 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 10000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-1 / Wavelength: 0.934 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Sep 7, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→20 Å / Num. all: 50720 / Num. obs: 49706 / % possible obs: 98.8 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 1 / Redundancy: 6.1 % / Biso Wilson estimate: 20 Å2 / Rmerge(I) obs: 0.04 / Net I/σ(I): 10 |
Reflection shell | Resolution: 1.65→1.71 Å / Rmerge(I) obs: 0.149 / Mean I/σ(I) obs: 6.5 / Num. unique all: 4908 / % possible all: 97.1 |
Reflection | *PLUS Lowest resolution: 20 Å / Num. measured all: 304335 / Rmerge(I) obs: 0.04 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1QKJ Resolution: 1.65→20 Å / σ(F): 2 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.65→20 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / σ(F): 2 / % reflection Rfree: 5 % / Rfactor obs: 0.2 / Rfactor Rwork: 0.2 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |