TRANSCRIPTION/DNA / MALE SEX DETERMINING FACTOR / SRY / SEX-REVERSAL MUTATION / DNA BENDING MUTANT / DIPOLAR COUPLINGS / MULTIDIMENSIONAL NMR / TRANSCRIPTION-DNA COMPLEX
機能・相同性
機能・相同性情報
positive regulation of male gonad development / Transcriptional regulation of testis differentiation / sex differentiation / male sex determination / anatomical structure morphogenesis / Deactivation of the beta-catenin transactivating complex / neuron differentiation / brain development / DNA-binding transcription activator activity, RNA polymerase II-specific / DNA-binding transcription factor binding ...positive regulation of male gonad development / Transcriptional regulation of testis differentiation / sex differentiation / male sex determination / anatomical structure morphogenesis / Deactivation of the beta-catenin transactivating complex / neuron differentiation / brain development / DNA-binding transcription activator activity, RNA polymerase II-specific / DNA-binding transcription factor binding / calmodulin binding / DNA-binding transcription factor activity, RNA polymerase II-specific / nuclear speck / RNA polymerase II cis-regulatory region sequence-specific DNA binding / positive regulation of gene expression / chromatin / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus / cytoplasm 類似検索 - 分子機能
Transcription factor SRY / High mobility group box domain / DNA Binding (I), subunit A / HMG (high mobility group) box / HMG boxes A and B DNA-binding domains profile. / high mobility group / High mobility group box domain / High mobility group box domain superfamily / Orthogonal Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
DNA / DNA (> 10) / Sex-determining region Y protein 類似検索 - 構成要素
Text: THE FOLLOWING EXPERIMENTS WERE CONDUCTED: (1) DOUBLE AND TRIPLE RESONANCE FOR ASSIGNMENT OF PROTEIN; (2) DOUBLE RESONANCE AND HETERONUCLEAR FILTERED FOR DNA; (3) QUANTITATIVE J CORRELATION FOR ...Text: THE FOLLOWING EXPERIMENTS WERE CONDUCTED: (1) DOUBLE AND TRIPLE RESONANCE FOR ASSIGNMENT OF PROTEIN; (2) DOUBLE RESONANCE AND HETERONUCLEAR FILTERED FOR DNA; (3) QUANTITATIVE J CORRELATION FOR COUPLING CONSTANTS; (4) 2D, 3D AND 4D HETERONUCLEAR SEPARATED AND FILTERED NOE EXPERIMENTS; (4) 2D and 3D DOUBLE AND TRIPLE RESONANCE EXPERIMENTS FOR DIPOLAR COUPLING MEASUREMENTS IN LIQUID CRYSTALLINE MEDIUM OF 4.5-5% 3:1 DMPC:DHPC
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試料調製
試料状態
イオン強度: 50 mM SODIUM PHOSPHATE / pH: 7.2 / 温度: 308.00 K
ソフトェア番号: 1 詳細: THE STRUCTURE WAS CALCULATED BY SIMULATED ANNEALING IN TORSION ANGLE SPACE (C. SCHWIETERS AND G.M. CLORE. J. MAGN. RESON., IN PRESS). THE TARGET FUNCTION COMPRISES TERMS FOR NOE RESTRAINTS, ...詳細: THE STRUCTURE WAS CALCULATED BY SIMULATED ANNEALING IN TORSION ANGLE SPACE (C. SCHWIETERS AND G.M. CLORE. J. MAGN. RESON., IN PRESS). THE TARGET FUNCTION COMPRISES TERMS FOR NOE RESTRAINTS, TORSION ANGLE RESTRAINTS, CARBON CHEMICAL SHIFT RESTRAINTS (KUSZWESKI ET AL. J. MAGN. RESON. SERIES B 106, 92-96 (1995), J COUPLING RESTRAINTS (GARRETT ET AL. J. MAGN. RESON. SERIES B 104, 99, 103 (1994); DIPOLAR COUPLING RESTRAINTS (CLORE ET AL. J.MAGN.RESON. 131, 159-162 (1998); J.MAGN.RESON. 133, 216-221(1998)), AND RADIUS OF GYRATION (KUSZEWSKI ET AL. JACS 121, 2337 (1999)). THE NON-BONDED CONTACTS ARE REPRESENTED BY A QUARTIC VAN DER WAALS REPULSION TERM (NILGES ET AL. (1988) FEBS LETT. 229, 129-136); THE DELPHIC TORSION ANGLE DATABASE POTENTIAL (KUSZEWSKI & C J. MAGN. RESON. 146, 249 (2000)); AND THE DELPHIC BASE-BASE POSITIONAL DATABASE POTENTIAL (KUSZEWSKI ET AL. JACS 123, 3903 (2001)). IN THIS ENTRY THE SECOND TO LAST COLUMN REPRESENTS THE AVERAGE RMS DIFFERENCE BETWEEN THE INDIVIDUAL SIMULATED ANNEALING STRUCTURES (400 FOR THE WILD TYPE COMPLEX) AND THE MEAN COORDINATE POSITIONS (OBTAINED BY BEST FITTING TO RESIDUES 4-81 OF THE PROTEIN AND 101-128 OF THE DNA). WILD TYPE M9I MUTANT PDB ID: 1J46 1J47 DEVIATIONS FROM IDEALIZED GEOMETRY: BONDS 0.003 A 0.003 A ANGLES 0.81 DEG 0.80 DEG IMPROPERS 0.79 DEG 0.79 DEG DEVIATIONS FROM EXPT RESTRAINTS NOES (1795/1693) 0.04 A 0.03 A TORSION ANGLES (433/429) 0.29 DEG 0.30 DEG 3JHNA COUPLINGS (70/66) 0.84 HZ 0.90 HZ 13C CHEMICAL SHIFTS (165/165) 0.99 PPM 0.95 PPM HETERONUCLEAR DIPOLAR COUPLING R-FACTORS (CLORE AND GARRETT J. AM. CHEM. SOC. 121, 9008-9012): PROTEIN 1DNH (71/66) 5.5% 7.6% PROTEIN 1DCH (67/67) 6.3% 10.0% PROTEIN 1DNC' (68/62) 18.9% 28.9% PROTEIN 2DHNC'(68/62) 18.8% 21.6% DNA 1DNH (9/10) 10.2% 16.1% DNA 1DCH (37/33) 11.2% 10.7% DNA 1H-1H DIPOLAR COUPLINGS (55/53) 0.56 HZ 0.75 HZ % RESIDUES IN MOST FAVORABLE REGION OF RAMACHADRAN MAP 94.7% 94.7%
NMRアンサンブル
コンフォーマー選択の基準: RESTRAINED REGULARIZED MEAN STRUCTURE 計算したコンフォーマーの数: 400 / 登録したコンフォーマーの数: 1