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Yorodumi- PDB-1iih: STRUCTURE OF TRYPANOSOMA BRUCEI BRUCEI TRIOSEPHOSPHATE ISOMERASE ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1iih | ||||||
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Title | STRUCTURE OF TRYPANOSOMA BRUCEI BRUCEI TRIOSEPHOSPHATE ISOMERASE COMPLEXED WITH 3-PHOSPHOGLYCERATE | ||||||
Components | TRIOSEPHOSPHATE ISOMERASE | ||||||
Keywords | ISOMERASE / TIM ligand complex | ||||||
Function / homology | Function and homology information glycosome / triose-phosphate isomerase / triose-phosphate isomerase activity / glyceraldehyde-3-phosphate biosynthetic process / glycerol catabolic process / gluconeogenesis / glycolytic process / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Trypanosoma brucei brucei (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.2 Å | ||||||
Authors | Noble, M.E. / Wierenga, R.K. / Lambeir, A.M. / Opperdoes, F.R. / Thunnissen, A.M. / Kalk, K.H. / Groendijk, H. / Hol, W.G.J. | ||||||
Citation | Journal: Proteins / Year: 1991 Title: The adaptability of the active site of trypanosomal triosephosphate isomerase as observed in the crystal structures of three different complexes. Authors: Noble, M.E. / Wierenga, R.K. / Lambeir, A.M. / Opperdoes, F.R. / Thunnissen, A.M. / Kalk, K.H. / Groendijk, H. / Hol, W.G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1iih.cif.gz | 107.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1iih.ent.gz | 83.3 KB | Display | PDB format |
PDBx/mmJSON format | 1iih.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1iih_validation.pdf.gz | 396 KB | Display | wwPDB validaton report |
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Full document | 1iih_full_validation.pdf.gz | 409.2 KB | Display | |
Data in XML | 1iih_validation.xml.gz | 13 KB | Display | |
Data in CIF | 1iih_validation.cif.gz | 20 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ii/1iih ftp://data.pdbj.org/pub/pdb/validation_reports/ii/1iih | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 26865.832 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Species: Trypanosoma brucei / Strain: brucei / References: UniProt: P04789, triose-phosphate isomerase #2: Chemical | ChemComp-3PG / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.38 Å3/Da / Density % sol: 48.31 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ROTATING ANODE / Type: ELLIOTT GX-21 / Wavelength: 1.5418 |
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Detector | Type: ENRAF-NONIUS FAST / Detector: DIFFRACTOMETER |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. all: 18169 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.051 |
Reflection | *PLUS Lowest resolution: 50 Å / % possible obs: 72.8 % |
Reflection shell | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 2.4 Å |
-Processing
Software |
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Refinement | Resolution: 2.2→50 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.2→50 Å
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Refine LS restraints |
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Software | *PLUS Name: 'TNT, GROMOS' / Classification: refinement | ||||||||||||||||
Refinement | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 50 Å / σ(F): 0 / Rfactor all: 0.14 | ||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||
Refine LS restraints | *PLUS
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