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Yorodumi- PDB-1ig8: Crystal Structure of Yeast Hexokinase PII with the correct amino ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ig8 | ||||||
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Title | Crystal Structure of Yeast Hexokinase PII with the correct amino acid sequence | ||||||
Components | hexokinase PII | ||||||
Keywords | TRANSFERASE / mixed alpha beta / two domains / cleft | ||||||
Function / homology | Function and homology information fructose import across plasma membrane / Regulation of Glucokinase by Glucokinase Regulatory Protein / regulation of transcription by glucose / hexokinase activity / Glycolysis / mannokinase activity / hexokinase / fructokinase activity / carbohydrate phosphorylation / glucokinase activity ...fructose import across plasma membrane / Regulation of Glucokinase by Glucokinase Regulatory Protein / regulation of transcription by glucose / hexokinase activity / Glycolysis / mannokinase activity / hexokinase / fructokinase activity / carbohydrate phosphorylation / glucokinase activity / mannose metabolic process / glucose 6-phosphate metabolic process / glucose binding / fructose metabolic process / regulation of cell size / glucose import / intracellular glucose homeostasis / negative regulation of apoptotic signaling pathway / Neutrophil degranulation / mitochondrial membrane / glycolytic process / glucose metabolic process / mitochondrion / ATP binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||
Authors | Kuser, P.R. / Krauchenco, S. / Antunes, O.A. / Polikarpov, I. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2000 Title: The high resolution crystal structure of yeast hexokinase PII with the correct primary sequence provides new insights into its mechanism of action. Authors: Kuser, P.R. / Krauchenco, S. / Antunes, O.A. / Polikarpov, I. #1: Journal: Acta Crystallogr.,Sect.D / Year: 1999 Title: Crystallization and Preliminary Crystal Analysis of Yeast Hexokinase PI and PII Authors: Kuser, P.R. / Golubev, A.M. / Polikarpov, I. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ig8.cif.gz | 113.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ig8.ent.gz | 87.7 KB | Display | PDB format |
PDBx/mmJSON format | 1ig8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ig/1ig8 ftp://data.pdbj.org/pub/pdb/validation_reports/ig/1ig8 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 54001.242 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P04807, hexokinase |
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#2: Chemical | ChemComp-SO4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.76 Å3/Da / Density % sol: 55.41 % | |||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 2.3-3.2M Ammonium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K | |||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: SYNCHROTRON / Site: LNLS / Beamline: D03B-MX1 / Wavelength: 1.38 Å |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 10, 1998 |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.38 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→13 Å / Num. obs: 60395 / % possible obs: 95.1 % / Redundancy: 2.12 % / Biso Wilson estimate: 23.13 Å2 / Rsym value: 0.113 |
Reflection shell | Resolution: 2.2→2.25 Å / Redundancy: 1.82 % / Rmerge(I) obs: 0.42 / Num. unique all: 1914 / % possible all: 96.6 |
Reflection | *PLUS Rmerge(I) obs: 0.113 |
Reflection shell | *PLUS % possible obs: 96.6 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: P152K mutant of yeast hexokinase Resolution: 2.2→13 Å / SU B: 6.1415 / SU ML: 0.15315 / Isotropic thermal model: Overall / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.24781 / ESU R Free: 0.22644 / Stereochemistry target values: Engh and Huber
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Displacement parameters | Biso mean: 23.4 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→13 Å
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Refine LS restraints |
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