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Yorodumi- PDB-1id1: CRYSTAL STRUCTURE OF THE RCK DOMAIN FROM E.COLI POTASSIUM CHANNEL -
+Open data
-Basic information
Entry | Database: PDB / ID: 1id1 | ||||||
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Title | CRYSTAL STRUCTURE OF THE RCK DOMAIN FROM E.COLI POTASSIUM CHANNEL | ||||||
Components | PUTATIVE POTASSIUM CHANNEL PROTEIN | ||||||
Keywords | MEMBRANE PROTEIN / RCK domain / E.coli Potassium Channel / BK Channel / Rossmann fold | ||||||
Function / homology | Function and homology information monoatomic ion transmembrane transport / potassium ion transport / protein-containing complex / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Jiang, Y. / Pico, A. / Cadene, M. / Chait, B.T. / MacKinnon, R. | ||||||
Citation | Journal: Neuron / Year: 2001 Title: Structure of the RCK domain from the E. coli K+ channel and demonstration of its presence in the human BK channel. Authors: Jiang, Y. / Pico, A. / Cadene, M. / Chait, B.T. / MacKinnon, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1id1.cif.gz | 66.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1id1.ent.gz | 50.7 KB | Display | PDB format |
PDBx/mmJSON format | 1id1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1id1_validation.pdf.gz | 430.3 KB | Display | wwPDB validaton report |
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Full document | 1id1_full_validation.pdf.gz | 439.7 KB | Display | |
Data in XML | 1id1_validation.xml.gz | 14.3 KB | Display | |
Data in CIF | 1id1_validation.cif.gz | 18.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/id/1id1 ftp://data.pdbj.org/pub/pdb/validation_reports/id/1id1 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 16746.068 Da / Num. of mol.: 2 / Fragment: C-TERMINAL DOMAIN, RESIDUES 241-393 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Plasmid: PET21B(+) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21DE3 / References: UniProt: P31069 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.53 Å3/Da / Density % sol: 65.16 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8 Details: PEG 4000, sodium chloride, tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1.1 Å |
Detector | Type: BRANDEIS / Detector: CCD / Date: May 1, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection shell | Resolution: 2.4→30 Å / Redundancy: 4.8 % / Rmerge(I) obs: 0.086 / % possible all: 99.5 |
Reflection | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 30 Å / % possible obs: 99.5 % / Redundancy: 4.8 % / Rmerge(I) obs: 0.086 |
Reflection shell | *PLUS % possible obs: 96.3 % / Rmerge(I) obs: 0.292 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.4→30 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.4→30 Å
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Refine LS restraints |
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