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Open data
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Basic information
Entry | Database: PDB / ID: 1ew3 | ||||||
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Title | CRYSTAL STRUCTURE OF THE MAJOR HORSE ALLERGEN EQU C 1 | ||||||
![]() | ALLERGEN EQU C 1 | ||||||
![]() | ALLERGEN / lipocalin / beta barrel | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Lascombe, M.B. / Gregoire, C. / Poncet, P. / Tavares, G.A. / Rosinski-Chupin, I. / Rabillon, J. / Goubran-Botros, H. / Mazie, J.C. / David, B. / Alzari, P.M. | ||||||
![]() | ![]() Title: Crystal structure of the allergen Equ c 1. A dimeric lipocalin with restricted IgE-reactive epitopes. Authors: Lascombe, M.B. / Gregoire, C. / Poncet, P. / Tavares, G.A. / Rosinski-Chupin, I. / Rabillon, J. / Goubran-Botros, H. / Mazie, J.C. / David, B. / Alzari, P.M. #1: ![]() Title: Crystallization and Preliminary Crystallographic Analysis of the Major Horse Allergen Equ c 1 Authors: Gregoire, C. / Tavares, G.A. / Lorenzo, H.K. / Dandeu, J.P. / David, B. / Alzari, P.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 42.9 KB | Display | ![]() |
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PDB format | ![]() | 33.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 371.6 KB | Display | ![]() |
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Full document | ![]() | 378.7 KB | Display | |
Data in XML | ![]() | 5.7 KB | Display | |
Data in CIF | ![]() | 8.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Details | The biological assembly is probably a dimer constructed from the given coordinates and a symmetry partner generated by the crystallographic two-fold axis |
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Components
#1: Protein | Mass: 18747.090 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.75 Å3/Da / Density % sol: 55.33 % | ||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: ammonium sulphate, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K | ||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Dec 7, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.2 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→20 Å / Num. all: 43339 / Num. obs: 9166 / % possible obs: 94.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 1 / Redundancy: 4.7 % / Biso Wilson estimate: 48 Å2 / Rmerge(I) obs: 0.082 / Net I/σ(I): 13.2 |
Reflection shell | Resolution: 2.3→2.4 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.431 / % possible all: 97.7 |
Reflection shell | *PLUS % possible obs: 97.7 % |
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Processing
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Refinement | Resolution: 2.3→15 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.3→15 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||
LS refinement shell | *PLUS Highest resolution: 2.3 Å / Lowest resolution: 2.4 Å / Rfactor Rfree: 0.331 / Rfactor Rwork: 0.21 / Num. reflection obs: 1119 |