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Open data
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Basic information
Entry | Database: PDB / ID: 1ic2 | ||||||
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Title | DECIPHERING THE DESIGN OF THE TROPOMYOSIN MOLECULE | ||||||
![]() | TROPOMYOSIN ALPHA CHAIN, SKELETAL MUSCLE | ||||||
![]() | CONTRACTILE PROTEIN / alpha-helical coiled coil / alanine / symmetry / axial stagger / bend | ||||||
Function / homology | ![]() Smooth Muscle Contraction / Striated Muscle Contraction / cardiac muscle contraction / cytoskeletal protein binding / actin filament / actin filament organization / actin filament binding / actin cytoskeleton / protein heterodimerization activity / protein homodimerization activity ...Smooth Muscle Contraction / Striated Muscle Contraction / cardiac muscle contraction / cytoskeletal protein binding / actin filament / actin filament organization / actin filament binding / actin cytoskeleton / protein heterodimerization activity / protein homodimerization activity / identical protein binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Brown, J.H. / Kim, K.-H. / Jun, G. / Greenfield, N.J. / Dominguez, R. / Volkmann, N. / Hitchcock-DeGregori, S.E. / Cohen, C. | ||||||
![]() | ![]() Title: Deciphering the design of the tropomyosin molecule Authors: Brown, J.H. / Kim, K.-H. / Jun, G. / Greenfield, N.J. / Dominguez, R. / Volkmann, N. / Hitchcock-DeGregori, S.E. / Cohen, C. #1: ![]() Title: Tropomyosin: Crystal Structure, Polymorphism and Molecular Interactions Authors: Caspar, D.L. / Cohen, C. / Longley, W. #2: ![]() Title: Tropomyosin Crystal Structure and Muscle Regulation Authors: Phillips Jr., G.N. / Fillers, J.P. / Cohen, C. #3: ![]() Title: The Structure of the N-terminus of Striated Muscle alpha-Tropomyosin in a Chimeric Peptide: Nuclear Magnetic Resonance Structure and Circular Dichroism Studies Authors: Greenfield, N.J. / Montelione, G.T. / Farid, R.S. / Hitchcock-DeGregori, S.E. #4: ![]() Title: Crystal Structure of Tropomyosin at 7 Angstroms Resolution Authors: Whitby, F.G. / Phillips Jr., G.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 74.7 KB | Display | ![]() |
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PDB format | ![]() | 58.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 420.8 KB | Display | ![]() |
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Full document | ![]() | 424.9 KB | Display | |
Data in XML | ![]() | 8.3 KB | Display | |
Data in CIF | ![]() | 12.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 9273.543 Da / Num. of mol.: 4 / Mutation: A81S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.73 Å3/Da / Density % sol: 54.89 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion / pH: 7.6 Details: PEG 200, isopropanol, magnesium chloride, ammonium acetate, TRIS, pH 7.6, VAPOR DIFFUSION, temperature 277K | ||||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS Density % sol: 56 % | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Details: used macroseeding | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2→50 Å / Num. all: 26797 / Num. obs: 26797 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 10 % / Biso Wilson estimate: 31.1 Å2 / Rmerge(I) obs: 0.087 / Net I/σ(I): 15.4 | ||||||||||||||||||||
Reflection shell | Resolution: 2→2.1 Å / Redundancy: 7.6 % / Rmerge(I) obs: 0.139 / Mean I/σ(I) obs: 10 / % possible all: 99.5 | ||||||||||||||||||||
Reflection | *PLUS |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 43.8603 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→50 Å
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Refine LS restraints |
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LS refinement shell |
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Software | *PLUS Name: CNS / Version: 0.9 / Classification: refinement | ||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2 Å / Lowest resolution: 50 Å / σ(F): 0 / % reflection Rfree: 5 % / Rfactor obs: 0.249 | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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