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- PDB-1hta: CRYSTAL STRUCTURE OF THE HISTONE HMFA FROM METHANOTHERMUS FERVIDUS -
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Open data
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Basic information
Entry | Database: PDB / ID: 1hta | ||||||
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Title | CRYSTAL STRUCTURE OF THE HISTONE HMFA FROM METHANOTHERMUS FERVIDUS | ||||||
![]() | HISTONE HMFA | ||||||
![]() | HISTONE | ||||||
Function / homology | ![]() DNA topological change / chromosome / double-stranded DNA binding / protein heterodimerization activity / protein homodimerization activity / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Decanniere, K. / Sandman, K. / Reeve, J.N. / Heinemann, U. | ||||||
![]() | ![]() Title: Crystal structures of recombinant histones HMfA and HMfB from the hyperthermophilic archaeon Methanothermus fervidus. Authors: Decanniere, K. / Babu, A.M. / Sandman, K. / Reeve, J.N. / Heinemann, U. #1: ![]() Title: Crystallization and Preliminary X-Ray Characterization of the Methanothermus Fervidus Histones Hmfa and Hmfb Authors: Decanniere, K. / Sandman, K. / Reeve, J.N. / Heinemann, U. #2: ![]() Title: NMR Structure of Hmfb from the Hyperthermophile, Methanothermus Fervidus, Confirms that This Archaeal Protein is a Histone Authors: Starich, M.R. / Sandman, K. / Reeve, J.N. / Summers, M.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 25.4 KB | Display | ![]() |
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PDB format | ![]() | 16.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 366.9 KB | Display | ![]() |
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Full document | ![]() | 366.6 KB | Display | |
Data in XML | ![]() | 3.1 KB | Display | |
Data in CIF | ![]() | 4.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 7513.844 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-CL / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 40 % Description: ORTHOROMBIC HMFA STRUCTURE WILL BE SUBMITTED SOON |
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 1, 1995 / Details: SEGMENTED MIRROR |
Radiation | Monochromator: BENT SINGLE-CRYSTAL GERMANIUM TRIANGULAR MONOCHROMATOR Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.937 Å / Relative weight: 1 |
Reflection | Resolution: 1.55→37.3 Å / Num. obs: 10817 / % possible obs: 98.2 % / Observed criterion σ(I): 0 / Redundancy: 7.1 % / Biso Wilson estimate: 22.87 Å2 / Rmerge(I) obs: 0.069 / Rsym value: 0.069 / Net I/σ(I): 4.8 |
Reflection shell | Resolution: 1.55→1.6 Å / Redundancy: 6.8 % / Rmerge(I) obs: 0.247 / Mean I/σ(I) obs: 3 / Rsym value: 0.247 / % possible all: 99 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PARTIALLY REFINED ORTHORHOMBIC HMFA Resolution: 1.55→18 Å / Cross valid method: AFTER RIGID BODY REFINEMENT / σ(F): 0 Details: RIGID BODY WITH AMORE, SIMULATED ANNEALING WITH X-PLOR, REFINEMENT WITH REFMAC. ESD FROM LUZZATI PLOT (A) : 0.2
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Displacement parameters | Biso mean: 25.34 Å2
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Refine analyze | Luzzati coordinate error obs: 0.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.55→18 Å
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Refine LS restraints |
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