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- PDB-1gjt: Solution structure of the Albumin binding domain of Streptococcal... -

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Basic information

Entry
Database: PDB / ID: 1gjt
TitleSolution structure of the Albumin binding domain of Streptococcal Protein G
ComponentsIMMUNOGLOBULIN G BINDING PROTEIN G
KeywordsIMMUNOGLOBULIN-BINDING PROTEIN / BACTERIAL SURFACE PROTEIN / ALBUMIN BINDING / PROTEIN G
Function / homology
Function and homology information


IgG binding / extracellular region
Similarity search - Function
Albumin-binding domain / IgG-binding B / B domain / M protein-type anchor domain / GA-like domain / GA-like domain / Immunoglobulin/albumin-binding domain superfamily / YSIRK Gram-positive signal peptide / LPXTG cell wall anchor motif / Gram-positive cocci surface proteins LPxTG motif profile. ...Albumin-binding domain / IgG-binding B / B domain / M protein-type anchor domain / GA-like domain / GA-like domain / Immunoglobulin/albumin-binding domain superfamily / YSIRK Gram-positive signal peptide / LPXTG cell wall anchor motif / Gram-positive cocci surface proteins LPxTG motif profile. / LPXTG cell wall anchor domain / Helicase, Ruva Protein; domain 3 / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Immunoglobulin G-binding protein G
Similarity search - Component
Biological speciesSTREPTOCOCCUS SP. 'GROUP G' (bacteria)
MethodSOLUTION NMR / DISTANCE GEOMETRY, RESTRAINED SIMULATED ANNEALING
Model type detailsMINIMIZED AVERAGE
AuthorsJohansson, M.U. / Frick, I.M. / Nilsson, H. / Kraulis, P.J. / Hober, S. / Jonasson, P. / Nygren, A.P. / Uhlen, M. / Bjorck, L. / Drakenberg, T. ...Johansson, M.U. / Frick, I.M. / Nilsson, H. / Kraulis, P.J. / Hober, S. / Jonasson, P. / Nygren, A.P. / Uhlen, M. / Bjorck, L. / Drakenberg, T. / Forsen, S. / Wikstrom, M.
CitationJournal: J.Biol.Chem. / Year: 2002
Title: Structure, Specificity, and Mode of Interaction for Bacterial Albumin-Binding Modules
Authors: Johansson, M. / Frick, I. / Nilsson, H. / Kraulis, P. / Hober, S. / Jonasson, P. / Linhult, M. / Nygren, P. / Uhlen, M. / Bjorck, L. / Drakenberg, T. / Forsen, S. / Wikstrom, M.
History
DepositionAug 2, 2001Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 9, 2001Provider: repository / Type: Initial release
Revision 1.1Apr 8, 2015Group: Derived calculations / Other ...Derived calculations / Other / Structure summary / Version format compliance

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: IMMUNOGLOBULIN G BINDING PROTEIN G


Theoretical massNumber of molelcules
Total (without water)7,1111
Polymers7,1111
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / -LEAST RESTRAINT VIOLATION
Representative

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Components

#1: Antibody IMMUNOGLOBULIN G BINDING PROTEIN G / ABD / ALBUMIN-BINDING DOMAIN OF PROTEIN G / IGG BINDING PROTEIN G


Mass: 7111.089 Da / Num. of mol.: 1 / Fragment: ALBUMIN-BINDING DOMAIN
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) STREPTOCOCCUS SP. 'GROUP G' (bacteria) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P19909
Sequence detailsRESIDUES 1 TO 19 ARE DERIVED FROM THE CLONING CONSTRUCT (KRAULIS ET AL., FEBS LETTERS (1996) 378, 190-194

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111HN(CA)CB
121CBCA(CO)NH
1313D HCC(CO)NH-TOCSY
1413D CC(CO)NH-TOCSY
15115N-
16113C EDITED 3D NOESY
NMR detailsText: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED ABD.

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Sample preparation

Sample conditionspH: 7.2 / Pressure: 1 atm / Temperature: 300 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometerType: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 600 MHz

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Processing

NMR software
NameVersionDeveloperClassification
X-PLOR3.8BRUNGERrefinement
XPLORstructure solution
RefinementMethod: DISTANCE GEOMETRY, RESTRAINED SIMULATED ANNEALING / Software ordinal: 1
Details: REFINEMENT CAN BE FOUND IN THE JRNL CITATION ABOVE.
NMR ensembleConformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers submitted total number: 1

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