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- PDB-1g94: CRYSTAL STRUCTURE ANALYSIS OF THE TERNARY COMPLEX BETWEEN PSYCHRO... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1g94 | |||||||||
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Title | CRYSTAL STRUCTURE ANALYSIS OF THE TERNARY COMPLEX BETWEEN PSYCHROPHILIC ALPHA AMYLASE FROM PSEUDOALTEROMONAS HALOPLANCTIS IN COMPLEX WITH A HEPTA-SACCHARIDE AND A TRIS MOLECULE | |||||||||
![]() | ALPHA-AMYLASE | |||||||||
![]() | HYDROLASE / beta-alpha-8-barrel / 3 domain structure | |||||||||
Function / homology | ![]() alpha-amylase / alpha-amylase activity / carbohydrate metabolic process / extracellular space / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Aghajari, N. / Roth, M. / Haser, R. | |||||||||
![]() | ![]() Title: Crystallographic evidence of a transglycosylation reaction: ternary complexes of a psychrophilic alpha-amylase. Authors: Aghajari, N. / Roth, M. / Haser, R. #1: ![]() Title: STRUCTURES OF THE PSYCHROPHILIC ALTEROMONAS HALOPLANCTIS ALPHA-AMYLASE GIVE INSIGHTS INTO COLD ADAPTATION AT A MOLECULAR LEVEL Authors: Aghajari, N. / Feller, G. / Gerday, C.H. / Haser, R. #2: ![]() Title: ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS IN ITS NATIVE FORM AND COMPLEXED WITH AN INHIBITOR Authors: Aghajari, N. / Feller, G. / Gerday, C.H. / Haser, R. #3: ![]() Title: Crystallization and preliminary X-ray diffraction studies of alpha-amylase from the antarctic psychrophile Alteromonas haloplanctis A23 Authors: Aghajari, N. / Feller, G. / Gerday, C.H. / Haser, R. | |||||||||
History |
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Remark 600 | HETEROGEN HET GROUP DAF USED IN THE FILE IS ALSO KNOWN AS: ACARVIOSINE (VALIENAMINE + 4-AMINO-4, 6- ...HETEROGEN HET GROUP DAF USED IN THE FILE IS ALSO KNOWN AS: ACARVIOSINE (VALIENAMINE + 4-AMINO-4, 6-DIDEOXY-A-D-GLUCOSE) |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 110.7 KB | Display | ![]() |
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PDB format | ![]() | 82.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1g9hC ![]() 1kxhC ![]() 1aqhS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 48947.426 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Sugars , 2 types, 2 molecules
#2: Polysaccharide | 4,6-dideoxy-4-{[(1S,5R,6S)-3-formyl-5,6-dihydroxy-4-oxocyclohex-2-en-1-yl]amino}-alpha-D-xylo-hex-5- ...4,6-dideoxy-4-{[(1S,5R,6S)-3-formyl-5,6-dihydroxy-4-oxocyclohex-2-en-1-yl]amino}-alpha-D-xylo-hex-5-enopyranose-(1-4)-alpha-D-glucopyranose Type: oligosaccharide / Mass: 477.417 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source |
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#3: Polysaccharide | 4,6-dideoxy-4-{[(1S,5R,6S)-3-formyl-5,6-dihydroxy-4-oxocyclohex-2-en-1-yl]amino}-alpha-D-xylo-hex-5- ...4,6-dideoxy-4-{[(1S,5R,6S)-3-formyl-5,6-dihydroxy-4-oxocyclohex-2-en-1-yl]amino}-alpha-D-xylo-hex-5-enopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose Type: oligosaccharide / Mass: 639.558 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source |
-Non-polymers , 4 types, 289 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/TRS.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/TRS.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-CA / |
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#5: Chemical | ChemComp-CL / |
#6: Chemical | ChemComp-TRS / |
#7: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.94 Å3/Da / Density % sol: 58.17 % | |||||||||||||||
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7 Details: MPD, Hepes, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 292K | |||||||||||||||
Crystal grow | *PLUS Details: Aghajari, N., (1996) Protein Sci., 5, 2128. | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: THOMSON/PRINCETON / Detector: CCD / Date: Feb 28, 1996 |
Radiation | Monochromator: null / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9828 Å / Relative weight: 1 |
Reflection | Resolution: 1.74→44.7 Å / Num. all: 46005 / Num. obs: 45586 / % possible obs: 77 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 2.4 % / Biso Wilson estimate: 16 Å2 / Rmerge(I) obs: 0.056 / Net I/σ(I): 9.7 |
Reflection | *PLUS % possible obs: 76.6 % / Num. measured all: 110412 |
Reflection shell | *PLUS % possible obs: 34.8 % |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1AQH Resolution: 1.74→44.7 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.74→44.7 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.74→1.83 Å / Total num. of bins used: 8
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Software | *PLUS Name: ![]() | |||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 44.7 Å / σ(F): 0 / % reflection Rfree: 10 % / Rfactor obs: 0.1564 / Rfactor Rfree: 0.1873 | |||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.267 / Rfactor Rwork: 0.264 |