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Open data
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Basic information
Entry | Database: PDB / ID: 1dzg | |||||||||
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Title | N135Q-S380C-ANTITHROMBIN-III | |||||||||
![]() | (ANTITHROMBIN- ...) x 2 | |||||||||
![]() | BLOOD CLOTTING / SERPIN | |||||||||
Function / homology | ![]() regulation of blood coagulation / Common Pathway of Fibrin Clot Formation / Intrinsic Pathway of Fibrin Clot Formation / Post-translational protein phosphorylation / serine-type endopeptidase inhibitor activity / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / blood coagulation / heparin binding / protease binding / collagen-containing extracellular matrix ...regulation of blood coagulation / Common Pathway of Fibrin Clot Formation / Intrinsic Pathway of Fibrin Clot Formation / Post-translational protein phosphorylation / serine-type endopeptidase inhibitor activity / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / blood coagulation / heparin binding / protease binding / collagen-containing extracellular matrix / blood microparticle / endoplasmic reticulum lumen / extracellular space / extracellular exosome / extracellular region / identical protein binding / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | McCoy, A.J. / Huntington, J.A. / Carrell, R.W. | |||||||||
![]() | ![]() Title: The Conformational Activation of Antithrombin. A 2. 85-A Structure of a Fluorescein Derivative Reveals an Electrostatic Link between the Hinge and Heparin Binding Regions. Authors: Huntington, J.A. / Mccoy, A.J. / Belzar, K.J. / Pei, X.Y. / Gettins, P.G.W. / Carrell, R.W. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 177.8 KB | Display | ![]() |
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PDB format | ![]() | 138.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1dzhC ![]() 2antS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-ANTITHROMBIN- ... , 2 types, 2 molecules IL
#1: Protein | Mass: 49131.105 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Details: INHIBITORY CONFORMATION / Source: (gene. exp.) ![]() Description: RECOMBINANT. BABY HAMSTER KIDNEY (BHK) CELL EXPRESSION Plasmid: PMASTOP / Production host: ![]() |
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#2: Protein | Mass: 49101.016 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: PLASMA ALPHA ANTITHROMBIN-III / Source: (natural) ![]() |
-Sugars , 2 types, 6 molecules ![](data/chem/img/NAG.gif)
#3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Sugar | |
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-Non-polymers , 2 types, 6 molecules ![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | #6: Water | ChemComp-HOH / | |
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-Details
Compound details | CHAIN I: HAS ENGINEERED MUTATIONS N135Q AND S380C N135Q MUTATION TO REMOVE GLYCOSYLATION SITE S380C ...CHAIN I: HAS ENGINEERED |
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Sequence details | ANT3_HUMAN RESIDUE NUMBERING IN SWS IS FROM START OF ANTITHROMBIN-III LEADER SEQUENCE. STRUCTURE ...ANT3_HUMAN RESIDUE NUMBERING IN SWS IS FROM START OF ANTITHROMB |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.17 Å3/Da / Density % sol: 62 % | ||||||||||||||||||||
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Crystal grow | pH: 6.5 Details: 5MG/ML 1:1 MIX OF INHIBITORY: LATENT ANTITHROMBIN-III, 10.5% PEG 4000, 65 MM NACACODYLATE, PH 6.5 | ||||||||||||||||||||
Crystal grow | *PLUS Method: batch method | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.88 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→20 Å / Num. obs: 98714 / % possible obs: 98.3 % / Observed criterion σ(I): 0 / Redundancy: 3.5 % / Rmerge(I) obs: 0.131 / Net I/σ(I): 7.4 |
Reflection | *PLUS Lowest resolution: 20 Å / Num. obs: 27983 / Num. measured all: 98714 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2ANT Resolution: 2.8→20 Å / SU B: 23.25 / SU ML: 0.4309 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 6.82723 / ESU R Free: 0.42762 / Details: BULK SOLVENT CORRECTION CALCULATED WITH CNS
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Displacement parameters | Biso mean: 66 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→20 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.228 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: p_bond_d / Dev ideal: 0.011 |