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- PDB-1kxh: Crystal structure of the complex between an inactive mutant of ps... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1kxh | |||||||||
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Title | Crystal structure of the complex between an inactive mutant of psychrophilic alpha-amylase (D174N) and acarbose | |||||||||
![]() | alpha-amylase | |||||||||
![]() | HYDROLASE / (beta/alpha)8 barrel | |||||||||
Function / homology | ![]() alpha-amylase / alpha-amylase activity / carbohydrate metabolic process / extracellular space / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Aghajari, N. / Haser, R. | |||||||||
![]() | ![]() Title: Crystallographic evidence of a transglycosylation reaction: ternary complexes of a psychrophilic alpha-amylase. Authors: Aghajari, N. / Roth, M. / Haser, R. #1: ![]() Title: Structures of the psychrophilic Alteromonas haloplanctis alpha-amylase give insights into cold adaptation at a molecular level #2: ![]() Title: Crystal structures of the psychrophilic alpha-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 106.2 KB | Display | ![]() |
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PDB format | ![]() | 78.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 766.9 KB | Display | ![]() |
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Full document | ![]() | 767.2 KB | Display | |
Data in XML | ![]() | 19.2 KB | Display | |
Data in CIF | ![]() | 27.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1g94C ![]() 1g9hC ![]() 1aqmS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 48946.438 Da / Num. of mol.: 1 / Mutation: D174N Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() |
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#2: Polysaccharide | 4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D- ...4,6-dideoxy-4-{[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose / alpha-acarbose |
#3: Chemical | ChemComp-CA / |
#4: Chemical | ChemComp-CL / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.93 Å3/Da / Density % sol: 58 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: MPD, hepes, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Apr 7, 1999 / Details: mirrors |
Radiation | Monochromator: OSMIC MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→29.7 Å / Num. all: 24442 / Num. obs: 24442 / % possible obs: 95.6 % / Observed criterion σ(I): 5 / Redundancy: 3.9 % / Biso Wilson estimate: 24.17 Å2 / Rmerge(I) obs: 0.092 / Net I/σ(I): 11.2 |
Reflection shell | Resolution: 2.3→2.36 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.309 / Mean I/σ(I) obs: 4.7 / Num. unique all: 2071 / % possible all: 91.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1AQM Resolution: 2.3→27.7 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.3→27.7 Å
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Refine LS restraints |
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