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- PDB-1jd9: CRYSTAL STRUCTURE ANALYSIS OF THE MUTANT K300Q OF PSEUDOALTEROMON... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1jd9 | ||||||
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Title | CRYSTAL STRUCTURE ANALYSIS OF THE MUTANT K300Q OF PSEUDOALTEROMONAS HALOPLANCTIS ALPHA-AMYLASE | ||||||
![]() | ALPHA-AMYLASE![]() | ||||||
![]() | ![]() ![]() ![]() | ||||||
Function / homology | ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Aghajari, N. / Haser, R. | ||||||
![]() | ![]() Title: Structural basis of alpha-amylase activation by chloride Authors: Aghajari, N. / Feller, G. / Gerday, C. / Haser, R. #1: ![]() Title: Structures of the psychrophilic Alteromonas haloplanctis alpha-amylase give insights into cold adaptation at a molecular level Authors: Aghajari, N. / Feller, G. / Gerday, C. / Haser, R. #2: ![]() Title: Crystal structures of the psychrophilic alpha-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor Authors: Aghajari, N. / Feller, G. / Gerday, C. / Haser, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 103.3 KB | Display | ![]() |
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PDB format | ![]() | 77.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1jd7C ![]() 1l0pC ![]() 1aqhS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | ![]() Mass: 49379.789 Da / Num. of mol.: 1 / Mutation: K300Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: A23 / Gene: AMY / Plasmid: PAH12WT / Gene (production host): HB101 / Production host: ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-CA / |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.89 Å3/Da / Density % sol: 57.44 % |
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Crystal grow![]() | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7 Details: MPD, Hepes, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
-Data collection
Diffraction | Mean temperature: 288 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Dec 29, 1997 / Details: collimator |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.5→43.03 Å / Num. all: 16759 / Num. obs: 16759 / % possible obs: 81.8 % / Observed criterion σ(F): 4 / Observed criterion σ(I): 4 / Redundancy: 3.4 % / Biso Wilson estimate: 32.92 Å2 / Rmerge(I) obs: 0.216 / Net I/σ(I): 3 |
Reflection shell | Resolution: 2.5→2.64 Å / Redundancy: 1.4 % / Rmerge(I) obs: 0.564 / Num. unique all: 1146 / % possible all: 39.2 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1AQH Resolution: 2.5→42.7 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.5→42.7 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.53 Å /
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