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- PDB-1fse: CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS REGULATORY PROTEIN GERE -
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Open data
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Basic information
Entry | Database: PDB / ID: 1fse | ||||||
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Title | CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS REGULATORY PROTEIN GERE | ||||||
![]() | GERE | ||||||
![]() | TRANSCRIPTION / Helix-turn-helix DNA-binding protein transcriptional regulator | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ducros, V.M.-A. / Lewis, R.J. / Verma, C.S. / Dodson, E.J. / Leonard, G. / Turkenburg, J.P. / Murshudov, G.N. / Wilkinson, A.J. / Brannigan, J.A. | ||||||
![]() | ![]() Title: Crystal structure of GerE, the ultimate transcriptional regulator of spore formation in Bacillus subtilis. Authors: Ducros, V.M. / Lewis, R.J. / Verma, C.S. / Dodson, E.J. / Leonard, G. / Turkenburg, J.P. / Murshudov, G.N. / Wilkinson, A.J. / Brannigan, J.A. #1: ![]() Title: Bacillus subtilis regulatory protein GerE Authors: Ducros, V. / Brannigan, J.A. / Lewis, R.J. / Wilkinson, A.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 93.5 KB | Display | ![]() |
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PDB format | ![]() | 73.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Details | There six monomers in the asymmetric unit arranged as three pairs of dimers (A and B, C and F, D and E) |
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Components
#1: Protein | Mass: 8603.060 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Chemical | ChemComp-SO4 / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.06 % | ||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 5 Details: Peg4000, sodium acetate, lithium or ammonium sulfate, pH 5, VAPOR DIFFUSION, HANGING DROP | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 291 K | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC / Detector: IMAGE PLATE / Date: Feb 15, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 |
Reflection | Resolution: 2.05→15 Å / Num. all: 25472 / Num. obs: 25472 / % possible obs: 99 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.11 % / Biso Wilson estimate: 32 Å2 / Rmerge(I) obs: 0.068 / Net I/σ(I): 11.53 |
Reflection shell | Resolution: 2.05→2.1 Å / Redundancy: 2.83 % / Rmerge(I) obs: 0.39 / Mean I/σ(I) obs: 4.07 / Num. unique all: 2421 / % possible all: 95.5 |
Reflection | *PLUS Num. obs: 29311 / Num. measured all: 91541 |
Reflection shell | *PLUS % possible obs: 95.5 % |
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Processing
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Refinement | Method to determine structure: ![]() Details: Used Translation, Libration and Screw motion (TLS) approach to refinement
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Refinement step | Cycle: LAST / Resolution: 2.05→20 Å
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Refine LS restraints |
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