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- PDB-1huw: THE CRYSTAL STRUCTURE OF AFFINITY-MATURED HUMAN GROWTH HORMONE AT... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1huw | ||||||
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Title | THE CRYSTAL STRUCTURE OF AFFINITY-MATURED HUMAN GROWTH HORMONE AT 2 ANGSTROMS RESOLUTION | ||||||
![]() | HUMAN GROWTH HORMONE | ||||||
![]() | HORMONE | ||||||
Function / homology | ![]() growth hormone receptor complex / bone maturation / prolactin receptor binding / positive regulation of growth / animal organ development / : / positive regulation of multicellular organism growth / positive regulation of glucose transmembrane transport / positive regulation of insulin-like growth factor receptor signaling pathway / growth hormone receptor binding ...growth hormone receptor complex / bone maturation / prolactin receptor binding / positive regulation of growth / animal organ development / : / positive regulation of multicellular organism growth / positive regulation of glucose transmembrane transport / positive regulation of insulin-like growth factor receptor signaling pathway / growth hormone receptor binding / growth hormone receptor signaling pathway / Prolactin receptor signaling / cell surface receptor signaling pathway via JAK-STAT / Synthesis, secretion, and deacylation of Ghrelin / Growth hormone receptor signaling / positive regulation of tyrosine phosphorylation of STAT protein / response to nutrient levels / positive regulation of MAP kinase activity / cytokine activity / endosome lumen / positive regulation of receptor signaling pathway via JAK-STAT / growth factor activity / hormone activity / cytokine-mediated signaling pathway / response to estradiol / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / extracellular space / extracellular region / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Ultsch, M.H. / Somers, W.S. / Kossiakoff, A.A. / De Vos, A.M. | ||||||
![]() | ![]() Title: The crystal structure of affinity-matured human growth hormone at 2 A resolution. Authors: Ultsch, M.H. / Somers, W. / Kossiakoff, A.A. / de Vos, A.M. #1: ![]() Title: Affinity Maturation of Human Growth Hormone by Monovalent Phage Display Authors: Lowman, H.B. / Wells, J.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 43.1 KB | Display | ![]() |
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PDB format | ![]() | 33.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 363.8 KB | Display | ![]() |
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Full document | ![]() | 366.5 KB | Display | |
Data in XML | ![]() | 5 KB | Display | |
Data in CIF | ![]() | 7.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21986.627 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43.29 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 6.5 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 8 Å / Num. obs: 6630 / % possible obs: 97.3 % / Num. measured all: 16335 / Rmerge(I) obs: 0.069 |
Reflection shell | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 2.48 Å / % possible obs: 94.1 % / Num. unique obs: 626 / Num. measured obs: 1167 / Rmerge(I) obs: 0.144 |
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Processing
Software |
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Refinement | Resolution: 2→8 Å / Rfactor Rwork: 0.185 / Rfactor obs: 0.185 / σ(F): 2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 8 Å / Num. reflection all: 12423 / Num. reflection obs: 11341 / σ(F): 2 / Rfactor all: 0.199 / Rfactor obs: 0.185 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 2.63 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | *PLUS Highest resolution: 2 Å / Lowest resolution: 2.07 Å / Num. reflection Rfree: 4151 / Rfactor obs: 0.298 |