+Open data
-Basic information
Entry | Database: PDB / ID: 1fnu | ||||||
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Title | STRUCTURE OF STREPTOCOCCAL PYROGENIC EXOTOXIN A | ||||||
Components | EXOTOXIN TYPE A PRECURSOR (ALLELE 1) | ||||||
Keywords | TOXIN / superantigen / exotoxin A | ||||||
Function / homology | Function and homology information modulation of host virulence by virus / : / toxin activity / extracellular region Similarity search - Function | ||||||
Biological species | Streptococcus pyogenes phage T12 (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.94 Å | ||||||
Authors | Earhart, C.A. / Vath, G.M. / Roggiani, M. / Schlievert, P.M. / Ohlendorf, D.H. | ||||||
Citation | Journal: Protein Sci. / Year: 2000 Title: Structure of streptococcal pyrogenic exotoxin A reveals a novel metal cluster. Authors: Earhart, C.A. / Vath, G.M. / Roggiani, M. / Schlievert, P.M. / Ohlendorf, D.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fnu.cif.gz | 201.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fnu.ent.gz | 166.3 KB | Display | PDB format |
PDBx/mmJSON format | 1fnu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1fnu_validation.pdf.gz | 394.6 KB | Display | wwPDB validaton report |
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Full document | 1fnu_full_validation.pdf.gz | 435.2 KB | Display | |
Data in XML | 1fnu_validation.xml.gz | 24.5 KB | Display | |
Data in CIF | 1fnu_validation.cif.gz | 38.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fn/1fnu ftp://data.pdbj.org/pub/pdb/validation_reports/fn/1fnu | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 25862.098 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Streptococcus pyogenes phage T12 (virus) / Species: Streptococcus pyogenes phage / Strain: PHAGE T12 / References: UniProt: P62560, UniProt: P0DJY8*PLUS #2: Chemical | ChemComp-CD / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 51.07 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop Details: 0.25 M LiCl, 5-20 mM CdCL2, 4-20% PEG 8000, 50 mM Na acetate, VAPOR DIFFUSION, HANGING DROP PH range: 3-7 | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 18 ℃ / pH: 7.5 | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 1.034 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.034 Å / Relative weight: 1 |
Reflection | Highest resolution: 1.94 Å / Num. obs: 74721 / % possible obs: 92 % / Rmerge(I) obs: 0.062 / Net I/σ(I): 10 |
Reflection | *PLUS % possible obs: 92 % |
-Processing
Software |
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Refinement | Resolution: 1.94→20 Å / σ(F): 1
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Refinement step | Cycle: LAST / Resolution: 1.94→20 Å
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Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / σ(F): 1 / Rfactor obs: 0.223 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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