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- PDB-1f9m: CRYSTAL STRUCTURE OF THIOREDOXIN F FROM SPINACH CHLOROPLAST (SHOR... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1f9m | ||||||
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Title | CRYSTAL STRUCTURE OF THIOREDOXIN F FROM SPINACH CHLOROPLAST (SHORT FORM) | ||||||
![]() | THIOREDOXIN F | ||||||
![]() | ELECTRON TRANSPORT | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Capitani, G. / Markovic-Housley, Z. / DelVal, G. / Morris, M. / Jansonius, J.N. / Schurmann, P. | ||||||
![]() | ![]() Title: Crystal structures of two functionally different thioredoxins in spinach chloroplasts. Authors: Capitani, G. / Markovic-Housley, Z. / DelVal, G. / Morris, M. / Jansonius, J.N. / Schurmann, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 53.1 KB | Display | ![]() |
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PDB format | ![]() | 42.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 420.7 KB | Display | ![]() |
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Full document | ![]() | 422.7 KB | Display | |
Data in XML | ![]() | 11.6 KB | Display | |
Data in CIF | ![]() | 15.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The biological assembly is a monomer |
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Components
#1: Protein | Mass: 12507.648 Da / Num. of mol.: 2 / Fragment: SHORT FORM Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.43 Å3/Da / Density % sol: 49.44 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.8 Details: ammonium sulphate, sodium acetate, PEG 4000, pH 5.80, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 7.3 | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Oct 2, 1995 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.86→25.5 Å / Num. obs: 20015 / % possible obs: 98.9 % / Redundancy: 2.9 % / Biso Wilson estimate: 19.8 Å2 / Rmerge(I) obs: 0.082 / Net I/σ(I): 5.7 |
Reflection shell | Resolution: 1.86→1.96 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.237 / % possible all: 95.3 |
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Processing
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Refinement | Resolution: 1.86→25.5 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber / Details: Used bulk solvent correction (X-PLOR 3.851)
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Refinement step | Cycle: LAST / Resolution: 1.86→25.5 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | |||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 25.5 Å / σ(F): 0 / % reflection Rfree: 4.8 % / Rfactor obs: 0.197 | |||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 22.3 Å2 | |||||||||||||||||||||
Refine LS restraints | *PLUS
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