negative regulation of glycine import across plasma membrane / negative regulation of dopamine receptor signaling pathway / negative regulation of potassium ion transmembrane transport / dorsal root ganglion development / negative regulation of cell growth involved in cardiac muscle cell development / negative regulation of G protein-coupled receptor signaling pathway / regulation of actin filament organization / regulation of potassium ion transmembrane transport / G alpha (q) signalling events / G alpha (i) signalling events ...negative regulation of glycine import across plasma membrane / negative regulation of dopamine receptor signaling pathway / negative regulation of potassium ion transmembrane transport / dorsal root ganglion development / negative regulation of cell growth involved in cardiac muscle cell development / negative regulation of G protein-coupled receptor signaling pathway / regulation of actin filament organization / regulation of potassium ion transmembrane transport / G alpha (q) signalling events / G alpha (i) signalling events / positive regulation of heart rate / regulation of calcium ion transport / positive regulation of excitatory postsynaptic potential / G-protein alpha-subunit binding / response to amphetamine / GTPase activator activity / response to cocaine / response to ethanol / protein kinase binding / protein-containing complex / nucleus / cytoplasm 類似検索 - 分子機能
Regulator of G-protein signalling 4, RGS domain / Regulator of G-protein Signalling 4; domain 1 - #10 / Regulator of G-protein Signalling 4; domain 1 / RGS, subdomain 1/3 / Regulator of G-protein Signalling 4, domain 2 / Regulator of G-protein Signalling 4; domain 2 / Regulator of G protein signaling domain / RGS, subdomain 2 / RGS domain / RGS domain profile. ...Regulator of G-protein signalling 4, RGS domain / Regulator of G-protein Signalling 4; domain 1 - #10 / Regulator of G-protein Signalling 4; domain 1 / RGS, subdomain 1/3 / Regulator of G-protein Signalling 4, domain 2 / Regulator of G-protein Signalling 4; domain 2 / Regulator of G protein signaling domain / RGS, subdomain 2 / RGS domain / RGS domain profile. / Regulator of G protein signalling domain / RGS domain superfamily / Orthogonal Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
structures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the least restraint violations,structures with the lowest energy,target function
代表モデル
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要素
#1: タンパク質
REGULATOROFG-PROTEINSIGNALING4 / RGS4
分子量: 19145.711 Da / 分子数: 1 / 断片: CORE DOMAIN OF RGS / 由来タイプ: 組換発現 / 詳細: SIX RESIDUE HISTIDINE TAG AT C-TERMINUS / 由来: (組換発現) Rattus norvegicus (ドブネズミ) / 器官: BRAIN / プラスミド: PRGS4 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P49799
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
2
1
2
3D 13C-separated NOESY
3
2
3
3D 13C-separated NOESY
3
3
3
HNHA
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試料調製
詳細
Solution-ID
内容
溶媒系
1
1mM RGS4 U-15N,13C; 50mM phosphate buffer, 2 mM NaN3, 50 mM deuterated DTT, pH 6.0
90% H2O/10% D2O
2
1mM RGS4 U-15N,13C; 50mM phosphate buffer, 2 mM NaN3, 50 mM deuterated DTT, pH 6.0
100% D2O
3
1mM RGS4 U-15N; 50mM phosphate buffer, 2 mM NaN3, 50 mM deuterated DTT, pH 6.0
90% H2O/10% D2O
結晶化
*PLUS
手法: other / 詳細: NMR
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NMR測定
NMRスペクトロメーター
タイプ: Bruker AMX / 製造業者: Bruker / モデル: AMX / 磁場強度: 600 MHz
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解析
NMR software
名称
バージョン
開発者
分類
X-PLOR
3.84
Brunger
構造決定
NMRPipe
1.7
Delaglio
解析
XwinNMR
2
Bruker
collection
PIPP
4.8.2
Garrett
データ解析
X-PLOR
3.84
Brunger
精密化
精密化
手法: distance geometry simulated annealing / ソフトェア番号: 1 詳細: the structures are based on a total of 2871 restraints, 3167 are NOE-derived distance constraints, 431 dihedral angle restraints,78 distance restraints from 39 backbone hydrogen bond, 132 ...詳細: the structures are based on a total of 2871 restraints, 3167 are NOE-derived distance constraints, 431 dihedral angle restraints,78 distance restraints from 39 backbone hydrogen bond, 132 3JHNa restraints, 136 Ca and 134 Cb chemical shift restraints and the use of a conformational database target function.
NMRアンサンブル
コンフォーマー選択の基準: structures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the least restraint violations,structures with the lowest ...コンフォーマー選択の基準: structures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the least restraint violations,structures with the lowest energy,target function 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 30