+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1e3y | ||||||
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タイトル | Death domain from human FADD/MORT1 | ||||||
要素 | FADD PROTEIN | ||||||
キーワード | APOPTOSIS / DEATH DOMAIN / ADAPTER MOLECULE / FAS RECEPTOR DEATH INDUCING SIGNALLING COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 positive regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation / negative regulation of activation-induced cell death of T cells / death effector domain binding / FasL/ CD95L signaling / tumor necrosis factor receptor superfamily binding / TRAIL signaling / CD95 death-inducing signaling complex / ripoptosome / TRAIL-activated apoptotic signaling pathway / death-inducing signaling complex assembly ...positive regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation / negative regulation of activation-induced cell death of T cells / death effector domain binding / FasL/ CD95L signaling / tumor necrosis factor receptor superfamily binding / TRAIL signaling / CD95 death-inducing signaling complex / ripoptosome / TRAIL-activated apoptotic signaling pathway / death-inducing signaling complex assembly / Defective RIPK1-mediated regulated necrosis / TRIF-mediated programmed cell death / TLR3-mediated TICAM1-dependent programmed cell death / Regulation by c-FLIP / CASP8 activity is inhibited / Dimerization of procaspase-8 / positive regulation of adaptive immune response / Caspase activation via Death Receptors in the presence of ligand / positive regulation of macrophage differentiation / necroptotic signaling pathway / caspase binding / NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 / death-inducing signaling complex / receptor serine/threonine kinase binding / negative regulation of necroptotic process / activation of cysteine-type endopeptidase activity / positive regulation of innate immune response / positive regulation of type I interferon-mediated signaling pathway / tumor necrosis factor receptor binding / death receptor binding / positive regulation of extrinsic apoptotic signaling pathway / motor neuron apoptotic process / TNFR1-induced proapoptotic signaling / RIPK1-mediated regulated necrosis / positive regulation of activated T cell proliferation / positive regulation of execution phase of apoptosis / T cell homeostasis / positive regulation of proteolysis / behavioral response to cocaine / extrinsic apoptotic signaling pathway via death domain receptors / lymph node development / signaling adaptor activity / extrinsic apoptotic signaling pathway / extrinsic apoptotic signaling pathway in absence of ligand / spleen development / thymus development / kidney development / positive regulation of interleukin-8 production / apoptotic signaling pathway / Regulation of TNFR1 signaling / Regulation of necroptotic cell death / cytoplasmic side of plasma membrane / cellular response to mechanical stimulus / positive regulation of T cell mediated cytotoxicity / positive regulation of tumor necrosis factor production / positive regulation of type II interferon production / cell body / T cell differentiation in thymus / positive regulation of canonical NF-kappaB signal transduction / defense response to virus / protease binding / molecular adaptor activity / positive regulation of apoptotic process / innate immune response / apoptotic process / protein-containing complex binding / positive regulation of transcription by RNA polymerase II / identical protein binding / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | HOMO SAPIENS (ヒト) | ||||||
手法 | 溶液NMR / SIMULATED ANNEALING FROM RANDOM CHAIN STARTING CONFORMERS | ||||||
Model type details | MINIMIZED AVERAGE | ||||||
データ登録者 | Driscoll, P.C. / Berglund, H. / Olerenshaw, D. / McDonald, N.Q. | ||||||
引用 | ジャーナル: J.Mol.Biol. / 年: 2000 タイトル: The Three-Dimensional Solution Structure and Dynamic Properties of the Human Fadd Death Domain 著者: Berglund, H. / Olerenshaw, D. / Sankar, A. / Federwisch, M. / Mcdonald, N.Q. / Driscoll, P.C. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1e3y.cif.gz | 46.9 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1e3y.ent.gz | 34.3 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1e3y.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1e3y_validation.pdf.gz | 336.7 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1e3y_full_validation.pdf.gz | 349.3 KB | 表示 | |
XML形式データ | 1e3y_validation.xml.gz | 5.4 KB | 表示 | |
CIF形式データ | 1e3y_validation.cif.gz | 6.8 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/e3/1e3y ftp://data.pdbj.org/pub/pdb/validation_reports/e3/1e3y | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 11870.372 Da / 分子数: 1 / 断片: DEATH DOMAIN RESIDUES 93-192 / 変異: YES / 由来タイプ: 組換発現 / 由来: (組換発現) HOMO SAPIENS (ヒト) 解説: PCR CLONING INTO N-TERMINAL 6*HIS TAG THROMBIN-CLEAVABLE FUSION PROTEIN プラスミド: PET14B / 発現宿主: ESCHERICHIA COLI (大腸菌) / 株 (発現宿主): BL21(DE3) / 参照: UniProt: Q13158 |
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構成要素の詳細 | CHIAN A EXTRA GLY-SER-HIS-MET- AT N-TERMINUS |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED FADD-DD. FULL DETAILS ARE GIVEN IN BERGLUND ET AL., J. MOL. BIOL. (2000), IN PRESS |
-試料調製
試料状態 | イオン強度: 50 MM PHOSPHATE BUFFER, 150MM NACL / pH: 6.2 / 圧: 1 atm / 温度: 298 K |
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結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
NMRスペクトロメーター | タイプ: Varian UNITYPLUS / 製造業者: Varian / モデル: UNITYPLUS / 磁場強度: 600 MHz |
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-解析
NMR software |
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精密化 | 手法: SIMULATED ANNEALING FROM RANDOM CHAIN STARTING CONFORMERS ソフトェア番号: 1 詳細: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: LEAST RESTRAINT VIOLATION AND GOOD NON-BONDED CONTACTS 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 1 |