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Yorodumi- PDB-1e3t: Solution Structure of the NADP(H) binding Component (dIII) of Pro... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1e3t | ||||||
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Title | Solution Structure of the NADP(H) binding Component (dIII) of Proton-Translocating Transhydrogenase from Rhodospirillum rubrum | ||||||
Components | NICOTINAMIDE NUCLEOTIDE TRANSHYDROGENASE (SUBUNIT BETA) | ||||||
Keywords | TRANSHYDROGENASE / MEMBRANE PROTEIN / PROTON TRANSLOCATION / NUCLEOTIDE BINDING | ||||||
Function / homology | Function and homology information NAD(P)+ transhydrogenase (AB-specific) activity / proton-translocating NAD(P)+ transhydrogenase / NADP binding / membrane => GO:0016020 / plasma membrane Similarity search - Function | ||||||
Biological species | RHODOSPIRILLUM RUBRUM (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model type details | MINIMIZED AVERAGE | ||||||
Authors | Jeeves, M. / Smith, K.J. / Quirk, P.G. / Cotton, N.P.J. / Jackson, J.B. | ||||||
Citation | Journal: Biochim.Biophys.Acta / Year: 2000 Title: Solution Structure of the Nadp(H)-Binding Component (Diii) of Proton-Translocating Transhydrogenase from Rhodospirillum Rubrum Authors: Jeeves, M. / Smith, K.J. / Quirk, P.G. / Cotton, N.P.J. / Jackson, J.B. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1e3t.cif.gz | 62.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1e3t.ent.gz | 50.3 KB | Display | PDB format |
PDBx/mmJSON format | 1e3t.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e3/1e3t ftp://data.pdbj.org/pub/pdb/validation_reports/e3/1e3t | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 21485.510 Da / Num. of mol.: 1 / Fragment: NADP(H) BINDING DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) RHODOSPIRILLUM RUBRUM (bacteria) / Gene: PNTB / Plasmid: PET11C / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) References: UniProt: Q59765, UniProt: Q2RSB4*PLUS, EC: 1.6.1.1 |
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#2: Chemical | ChemComp-NAP / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: AVERAGE STRUCTURE. THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON UNLABELLED, 15N LABELLED AND 13C, 15N-LABELLED DIII. |
-Sample preparation
Sample conditions | Ionic strength: 20 MM HEPES / pH: 7.2 / Pressure: 1 atm / Temperature: 303 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE BBA REFERNCE CITED. | ||||||||||||
NMR ensemble | Conformer selection criteria: AVERAGE OF 10 LOWEST ENERGY STRUCTURES Conformers calculated total number: 100 / Conformers submitted total number: 1 |