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- PDB-2qn4: Structure and function study of rice bifunctional alpha-amylase/s... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2qn4 | ||||||
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Title | Structure and function study of rice bifunctional alpha-amylase/subtilisin inhibitor from Oryza sativa | ||||||
![]() | Alpha-amylase/subtilisin inhibitor | ||||||
![]() | HYDROLASE INHIBITOR / RASI / amylase inhibitor / subtilisin inhibitor / Alpha-amylase inhibitor / Protease inhibitor / Serine protease inhibitor | ||||||
Function / homology | ![]() alpha-amylase inhibitor activity / serine-type endopeptidase inhibitor activity Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Peng, W.Y. / Lin, Y.H. / Huang, Y.C. / Guan, H.H. / Hsieh, Y.C. / Chen, C.J. | ||||||
![]() | ![]() Title: Structure and Function Study of Rice Bifunctional Alpha-Amylase/Subtilisin Inhibitor from Oryza Sativa Authors: Peng, W.Y. / Lin, Y.H. / Huang, Y.C. / Guan, H.H. / Hsieh, Y.C. / Liu, M.Y. / Chang, T. / Chen, C.J. #1: Journal: ACTA CRYSTALLOGR.,SECT.F / Year: 2006 Title: Purification, crystallization and preliminary X-ray crystallographic analysis of rice bifunctional alpha-amylase/subtilisin inhibitor from Oryza sativa Authors: Lin, Y.H. / Peng, W.Y. / Huang, Y.C. / Guan, H.H. / Hsieh, Y.C. / Liu, M.Y. / Chang, T. / Chen, C.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 77.9 KB | Display | ![]() |
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PDB format | ![]() | 57.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 443.8 KB | Display | ![]() |
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Full document | ![]() | 450.2 KB | Display | |
Data in XML | ![]() | 16.5 KB | Display | |
Data in CIF | ![]() | 23.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1avaS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21443.461 Da / Num. of mol.: 2 / Source method: isolated from a natural source Source: (natural) ![]() ![]() Strain: japonica cultivar-group / References: UniProt: P29421 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.96 Å3/Da / Density % sol: 37.17 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.7 Details: 20% PEG3350, 0.2M Potassium dihydrogen phosphate, pH4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Apr 6, 2006 Details: Vertically Collimating Premirror, LN2-Cooled Fixed-Exit Double Crystal Si(111) Monochromator, Toroidal Focusing Mirror |
Radiation | Monochromator: LN2-Cooled, Fixed-Exit Double Crystal Monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→30 Å / Num. obs: 31823 / % possible obs: 99.5 % / Redundancy: 4.7 % / Rsym value: 0.062 / Net I/σ(I): 22.5654 |
Reflection shell | Resolution: 1.8→1.86 Å / Redundancy: 4.7 % / Rmerge(I) obs: 0.269 / Mean I/σ(I) obs: 5.25 / Num. unique all: 3129 / Rsym value: 0.363 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1AVA Resolution: 1.8→30 Å / Isotropic thermal model: Anisotropic / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 0.16 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.8→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.86 Å
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