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Yorodumi- PDB-1a6w: B1-8 FV FRAGMENT COMPLEXED WITH A (4-HYDROXY-5-IODO-3-NITROPHENYL... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1a6w | ||||||
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Title | B1-8 FV FRAGMENT COMPLEXED WITH A (4-HYDROXY-5-IODO-3-NITROPHENYL) ACETATE COMPOUND | ||||||
Components |
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Keywords | IMMUNOGLOBULIN / HAPTEN | ||||||
Function / homology | Function and homology information immunoglobulin complex / adaptive immune response / immune response / extracellular space Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Simon, T. / Henrick, K. / Hirshberg, M. / Winter, G. | ||||||
Citation | Journal: To be Published Title: X-Ray Structures of Fv Fragment and its (4-Hydroxy-3-Nitrophenyl)Acetate Complex of Murine B1-8 Antibody Authors: Simon, T. / Henrick, K. / Hirshberg, M. / Winter, G. #1: Journal: Protein Eng. / Year: 1992 Title: A Functional Antibody Mutant with an Insertion in the Framework Region 3 Loop of the Vh Domain: Implications for Antibody Engineering Authors: Simon, T. / Rajewsky, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a6w.cif.gz | 57.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1a6w.ent.gz | 45.1 KB | Display | PDB format |
PDBx/mmJSON format | 1a6w.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1a6w_validation.pdf.gz | 443.9 KB | Display | wwPDB validaton report |
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Full document | 1a6w_full_validation.pdf.gz | 447.5 KB | Display | |
Data in XML | 1a6w_validation.xml.gz | 7 KB | Display | |
Data in CIF | 1a6w_validation.cif.gz | 11 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a6/1a6w ftp://data.pdbj.org/pub/pdb/validation_reports/a6/1a6w | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 11503.741 Da / Num. of mol.: 1 / Fragment: FV FRAGMENT / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Strain: C57BL-6 / References: UniProt: P01724 |
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#2: Antibody | Mass: 13440.945 Da / Num. of mol.: 1 / Fragment: FV FRAGMENT / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Strain: C57BL/6 / References: UniProt: P01751 |
#3: Chemical | ChemComp-NIP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.43 Å3/Da / Density % sol: 40.5 % |
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Crystal grow | Method: dialysis Details: THE PURIFIED PROTEIN HAS BEEN DIALYSED INTO A 10 MM TRIS-HCL PH 7.5 BUFFER ALSO CONTAINING 20 MM NACL. PEG 1550 WAS USED AS PRECIPITANT., dialysis |
-Data collection
Diffraction | Mean temperature: 269 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: XENTRONICS / Detector: AREA DETECTOR / Date: Aug 1, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→36 Å / Num. obs: 65498 / % possible obs: 95.1 % / Observed criterion σ(I): 0 / Redundancy: 2.9 % / Biso Wilson estimate: 18.5 Å2 / Rmerge(I) obs: 0.054 |
Reflection shell | Resolution: 1.8→1.85 Å / Redundancy: 1.6 % / Rmerge(I) obs: 0.301 / % possible all: 79.2 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→6 Å / σ(F): 0 /
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Displacement parameters | Biso mean: 19.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→6 Å
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Refine LS restraints |
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