+Open data
-Basic information
Entry | Database: PDB / ID: 1a6u | ||||||
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Title | B1-8 FV FRAGMENT | ||||||
Components |
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Keywords | IMMUNOGLOBULIN | ||||||
Function / homology | Function and homology information immunoglobulin complex / adaptive immune response / immune response / extracellular space Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.1 Å | ||||||
Authors | Simon, T. / Henrick, K. / Hirshberg, M. / Winter, G. | ||||||
Citation | Journal: To be Published Title: X-Ray Structures of Fv Fragment and its (4-Hydroxy-3-Nitrophenyl)Acetate Complex of Murine B1-8 Antibody Authors: Simon, T. / Henrick, K. / Hirshberg, M. / Winter, G. #1: Journal: Protein Eng. / Year: 1992 Title: A Functional Antibody Mutant with an Insertion in the Framework Region 3 Loop of the Vh Domain: Implications for Antibody Engineering Authors: Simon, T. / Rajewsky, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1a6u.cif.gz | 56.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1a6u.ent.gz | 42.9 KB | Display | PDB format |
PDBx/mmJSON format | 1a6u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a6/1a6u ftp://data.pdbj.org/pub/pdb/validation_reports/a6/1a6u | HTTPS FTP |
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-Related structure data
Related structure data | 1a6vC 1a6wC 1mfaS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 11374.628 Da / Num. of mol.: 1 / Fragment: FV FRAGMENT / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Strain: C57BL/6 / References: UniProt: P01724 |
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#2: Antibody | Mass: 13440.945 Da / Num. of mol.: 1 / Fragment: FV FRAGMENT / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / Strain: C57BL/6 / References: UniProt: P01751 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 24.4 % |
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Crystal grow | Details: CRYSTALS WERE GROWN USING AMMONIUM SULFATE AS PRECIPITANT. |
-Data collection
Diffraction | Mean temperature: 269 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 1, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→13.7 Å / Num. obs: 105196 / % possible obs: 92.3 % / Observed criterion σ(I): 0 / Redundancy: 4.3 % / Biso Wilson estimate: 23.8 Å2 / Rmerge(I) obs: 0.054 |
Reflection shell | Resolution: 2→2.05 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.177 / % possible all: 61.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1MFA Resolution: 2.1→6 Å / σ(F): 0 /
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Displacement parameters | Biso mean: 30.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→6 Å
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Refine LS restraints |
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