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- PDB-1a6v: B1-8 FV fragment complexed with a (4-hydroxy-3-nitrophenyl) aceta... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1a6v | |||||||||
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Title | B1-8 FV fragment complexed with a (4-hydroxy-3-nitrophenyl) acetate compound | |||||||||
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![]() | IMMUNE SYSTEM / IMMUNOGLOBULIN / HAPTEN | |||||||||
Function / homology | ![]() immunoglobulin complex / adaptive immune response / immune response / extracellular space Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Simon, T. / Henrick, K. / Hirshberg, M. / Winter, G. | |||||||||
![]() | ![]() Title: X-Ray Structures of Fv Fragment and its (4-Hydroxy-3-Nitrophenyl)Acetate Complex of Murine B1-8 Antibody Authors: Simon, T. / Henrick, K. / Hirshberg, M. / Winter, G. #1: ![]() Title: A Functional Antibody Mutant with an Insertion in the Framework Region 3 Loop of the Vh Domain: Implications for Antibody Engineering Authors: Simon, T. / Rajewsky, K. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 151.1 KB | Display | ![]() |
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PDB format | ![]() | 121.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 581 KB | Display | ![]() |
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Full document | ![]() | 630.7 KB | Display | |
Data in XML | ![]() | 22 KB | Display | |
Data in CIF | ![]() | 33.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1a6uC ![]() 1a6wC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Antibody | Mass: 11631.869 Da / Num. of mol.: 3 / Fragment: FV FRAGMENT Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Antibody | Mass: 13440.945 Da / Num. of mol.: 3 / Fragment: FV FRAGMENT Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 33.2 % |
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Crystal grow | Details: CRYSTALS WERE GROWN USING AMMONIUM SULPHATE AS PRECIPITANT |
-Data collection
Diffraction | Mean temperature: 269 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 1, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 |
Reflection | Resolution: 1.74→9.2 Å / Num. obs: 367775 / % possible obs: 94.8 % / Observed criterion σ(I): 0 / Redundancy: 5.9 % / Biso Wilson estimate: 14.9 Å2 / Rmerge(I) obs: 0.059 |
Reflection shell | Resolution: 1.8→1.88 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.361 / % possible all: 95.8 |
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Processing
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Refinement | Method to determine structure: ![]() Details: THE STRUCTURE HAS THREE MOLECULES PER EQUIVALENT POSITION ARRANGED AS A TRANSLATION ALONG THE Z-AXIS SUCH THAT OVERALL THE DIFFRACTION DATA SHOWS WEAK INTENSITIES FOR HKL, L=2N AND THIS ...Details: THE STRUCTURE HAS THREE MOLECULES PER EQUIVALENT POSITION ARRANGED AS A TRANSLATION ALONG THE Z-AXIS SUCH THAT OVERALL THE DIFFRACTION DATA SHOWS WEAK INTENSITIES FOR HKL, L=2N AND THIS TRANSLATIONAL NON-CRYSTALLOGRAPHIC SYMMETRY IS ON CLEARLY BROKEN BY THE DIFFERENT CONFORMATION OF THE TAIL O HAPTEN LIGAND ADOPTED IN EACH OF THE THREE INDEPENDENTLY DETERMINED MOLECULES. THERE ARE ALSO TWO CONFORMATIONS FOUND FOR THE HAPTEN BOUND TO CHAINS L AND H.
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Displacement parameters | Biso mean: 14.8 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→6 Å
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Refine LS restraints |
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