protein-RNA complex assembly / maturation of LSU-rRNA / cytosolic ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / modification-dependent protein catabolic process / protein tag activity / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly ...protein-RNA complex assembly / maturation of LSU-rRNA / cytosolic ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / modification-dependent protein catabolic process / protein tag activity / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / protein ubiquitination / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / ubiquitin protein ligase binding / RNA binding / zinc ion binding / nucleus / cytoplasm 類似検索 - 分子機能
Ribosomal protein L13e / Ribosomal protein L2, archaeal-type / : / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site / Ribosomal protein L13e / Ribosomal protein L30/YlxQ / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L22e ...Ribosomal protein L13e / Ribosomal protein L2, archaeal-type / : / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site / Ribosomal protein L13e / Ribosomal protein L30/YlxQ / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L10e / Ribosomal protein L19, eukaryotic / 60S ribosomal protein L18a/ L20, eukaryotes / : / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L5 eukaryotic, C-terminal / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal L18 C-terminal region / Ribosomal L40e family / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / : / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / 60S ribosomal protein L19 / : / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L34Ae / Ribosomal protein L34e / Ribosomal protein L7A/L8 / 60S ribosomal protein L6E / 60S ribosomal protein L35 / Ribosomal protein L18e / Ribosomal protein L19e, C-terminal domain / Ribosomal_L19e / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e, N-terminal domain / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / Ribosomal protein L37ae / Ribosomal L37ae protein family / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L14e domain / Ribosomal protein L14 / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L39e / Ribosomal protein L39e domain superfamily / Ribosomal protein L35A superfamily / Ribosomal L39 protein / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L14 / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L1e signature. / Ribosomal protein L31e / Ribosomal protein L31e domain superfamily / Ribosomal protein L31e / Ribosomal_L31e / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein L21e / Ribosomal protein L21e signature. / Ribosomal protein L24e-related / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L24e / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L37e, conserved site / Ribosomal protein L37e signature. / Ribosomal protein L37e / Ribosomal protein L37e / Ribosomal_L15e / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal L15 / Ribosomal protein L3, domain 3, archaeal type superfamily / Ribosomal protein L3, archaeal/eukaryotic type / Ribosomal protein L32e / Ribosomal protein L37ae/L37e / Ribosomal protein L32e superfamily 類似検索 - ドメイン・相同性
60S ribosomal protein L18, putative / 60S ribosomal protein L19-3, putative / 60S ribosomal protein L39, putative / Ribosomal protein L31e, putative / 60S ribosomal protein L44, putative / 60S ribosomal protein L3 / Ribosomal protein L6, putative / 60S ribosomal protein L37a / Ubiquitin, putative / Large ribosomal subunit protein uL15/eL18 domain-containing protein ...60S ribosomal protein L18, putative / 60S ribosomal protein L19-3, putative / 60S ribosomal protein L39, putative / Ribosomal protein L31e, putative / 60S ribosomal protein L44, putative / 60S ribosomal protein L3 / Ribosomal protein L6, putative / 60S ribosomal protein L37a / Ubiquitin, putative / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Ribosomal protein L24e, putative / Ribosomal protein L24, putative / 60S ribosomal protein L29 / Ribosomal protein L22, putative / Ribosomal protein L27e, putative / Ribosomal protein L5 / Large ribosomal subunit protein eL22 / Large ribosomal subunit protein uL14 / Ribosomal protein L35Ae, putative / Ribosomal protein L23, putative / Ribosomal protein L13, putative / Ribosomal protein L14, putative / Large ribosomal subunit protein uL4 / Ribosomal protein L29, putative / Ribosomal protein L8, putative / 60S ribosomal protein L30, putative / 60S ribosomal protein L11, putative / Ribosomal protein L15 / 60S ribosomal protein L18a / 60S ribosomal protein L7a / Ribosomal protein L36e, putative / Ribosomal protein L32, putative / Ribosomal protein L21e, putative / Ribosomal protein L30p/L7e, putative / Ribosomal protein L37 / Ribosomal protein L38e, putative / Ribosomal protein, putative / Ribosomal protein L34e, putative / 60S ribosomal protein L6, putative / Ribosomal protein L13e, putative 類似検索 - 構成要素
ジャーナル: Cell Res / 年: 2017 タイトル: Cryo-EM structures of the 80S ribosomes from human parasites Trichomonas vaginalis and Toxoplasma gondii. 著者: Zhifei Li / Qiang Guo / Lvqin Zheng / Yongsheng Ji / Yi-Ting Xie / De-Hua Lai / Zhao-Rong Lun / Xun Suo / Ning Gao / 要旨: As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High- ...As an indispensable molecular machine universal in all living organisms, the ribosome has been selected by evolution to be the natural target of many antibiotics and small-molecule inhibitors. High-resolution structures of pathogen ribosomes are crucial for understanding the general and unique aspects of translation control in disease-causing microbes. With cryo-electron microscopy technique, we have determined structures of the cytosolic ribosomes from two human parasites, Trichomonas vaginalis and Toxoplasma gondii, at resolution of 3.2-3.4 Å. Although the ribosomal proteins from both pathogens are typical members of eukaryotic families, with a co-evolution pattern between certain species-specific insertions/extensions and neighboring ribosomal RNA (rRNA) expansion segments, the sizes of their rRNAs are sharply different. Very interestingly, rRNAs of T. vaginalis are in size comparable to prokaryotic counterparts, with nearly all the eukaryote-specific rRNA expansion segments missing. These structures facilitate the dissection of evolution path for ribosomal proteins and RNAs, and may aid in design of novel translation inhibitors.