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- EMDB-4735: Structure of XBP1u-paused ribosome nascent chain complex with SRP. -

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Basic information

Entry
Database: EMDB / ID: EMD-4735
TitleStructure of XBP1u-paused ribosome nascent chain complex with SRP.
Map data
SampleStructure of XBP1u-paused ribosome nascent chain complex with SRP.
  • Structure of XBP1u-paused nascent chain complex with SRP.
  • P- and E- site tRNA
  • (X-box-binding protein ...) x 2
  • mRNA encoding XBP1u
  • SRP
  • (Signal sequence ...) x 2
  • eS31
  • (nucleic-acidNucleic acid) x 8
  • (Ribosomal protein ...) x 20
  • uS14
  • uL2
  • (40S ribosomal protein ...) x 12
  • uL3
  • uL4
  • (60S ribosomal protein ...) x 11
  • uL30
  • eL8
  • uL6
  • uS13
  • eS17
  • uL22
  • eS19
  • eL18
  • eL20
  • eS10
  • eL21
  • eL14
  • uS19
  • uL23
  • uL15
  • eL29
  • eL30
  • eL31
  • eL32CD59
  • eL33
  • eL34
  • uL29
  • eL39
  • eL42
  • uS2
  • eL28
  • 60S acidic ribosomal protein P0
  • uL11
  • uS5
  • (Signal recognition particle ...) x 5
  • (ligand) x 2
Function / homology
Function and homology information


SRP-dependent cotranslational protein targeting to membrane / XBP1(S) activates chaperone genes / IRE1alpha activates chaperones / ATF6 (ATF6-alpha) activates chaperone genes / positive regulation of plasma cell differentiation / epithelial cell maturation involved in salivary gland development / positive regulation of phospholipid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter / positive regulation of histone methylation / SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition / sterol homeostasis ...SRP-dependent cotranslational protein targeting to membrane / XBP1(S) activates chaperone genes / IRE1alpha activates chaperones / ATF6 (ATF6-alpha) activates chaperone genes / positive regulation of plasma cell differentiation / epithelial cell maturation involved in salivary gland development / positive regulation of phospholipid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter / positive regulation of histone methylation / SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition / sterol homeostasis / positive regulation of protein acetylation / organelle organization / positive regulation of vascular wound healing / negative regulation of pathway-restricted SMAD protein phosphorylation / endoplasmic reticulum signal peptide binding / positive regulation of ER-associated ubiquitin-dependent protein catabolic process / signal recognition particle binding / response to insulin-like growth factor stimulus / positive regulation of immunoglobulin production / signal recognition particle, endoplasmic reticulum targeting / positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response / ATF6-mediated unfolded protein response / negative regulation of translational elongation / positive regulation of lactation / exocrine pancreas development / protein targeting to ER / enhancer sequence-specific DNA binding / positive regulation of MHC class II biosynthetic process / negative regulation of myotube differentiation / cellular response to fructose stimulus / SRP-dependent cotranslational protein targeting to membrane, translocation / cellular triglyceride homeostasis / negative regulation of protein neddylation / positive regulation of hepatocyte proliferation / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / oxidized pyrimidine DNA binding / positive regulation of DNA N-glycosylase activity / positive regulation of base-excision repair / response to TNF agonist / negative regulation of DNA repair / positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis / positive regulation of immunoglobulin secretion / negative regulation of endoplasmic reticulum unfolded protein response / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / mammalian oogenesis stage / NF-kappaB complex / cellular response to nutrient / ubiquitin ligase inhibitor activity / negative regulation of RNA splicing / endothelial cell proliferation / positive regulation of TOR signaling / 7S RNA binding / positive regulation of T cell differentiation / positive regulation of endothelial cell apoptotic process / positive regulation of endodeoxyribonuclease activity / activation-induced cell death of T cells / positive regulation of endoplasmic reticulum unfolded protein response / protein kinase A binding / cellular response to laminar fluid shear stress / cytosolic ribosome / supercoiled DNA binding / positive regulation of B cell differentiation / ubiquitin-like protein conjugating enzyme binding / positive regulation of vascular associated smooth muscle cell migration / integral component of endoplasmic reticulum membrane / oxidized purine DNA binding / positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress / positive regulation of T cell receptor signaling pathway / muscle organ development / erythrocyte development / positive regulation of activated T cell proliferation / positive regulation of interleukin-6 secretion / adipose tissue development / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / ribonucleoprotein complex binding / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / T cell proliferation involved in immune response / cellular response to interleukin-4 / spindle assembly / ribosomal small subunit biogenesis / positive regulation of interleukin-2 production / neuron development / cellular response to peptide hormone stimulus / ribosomal large subunit biogenesis / SRP-dependent cotranslational protein targeting to membrane / protein localization to nucleus / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / positive regulation of JUN kinase activity / protein targeting / gastrulation / IRE1-mediated unfolded protein response / cellular response to glucose starvation / positive regulation of microtubule polymerization / phosphatidylinositol 3-kinase signaling / polysome / positive regulation of fat cell differentiation / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / endoplasmic reticulum unfolded protein response
Ribosomal protein L27e, conserved site / Ubiquitin conserved site / Ribosomal protein L11, N-terminal / Ribosomal protein L11, C-terminal / Ribosomal protein S7, conserved site / Ribosomal protein S9, conserved site / Ribosomal protein S5 domain 2-type fold / G-protein beta WD-40 repeat / Ribosomal protein L39e, conserved site / Ribosomal protein L31e, conserved site ...Ribosomal protein L27e, conserved site / Ubiquitin conserved site / Ribosomal protein L11, N-terminal / Ribosomal protein L11, C-terminal / Ribosomal protein S7, conserved site / Ribosomal protein S9, conserved site / Ribosomal protein S5 domain 2-type fold / G-protein beta WD-40 repeat / Ribosomal protein L39e, conserved site / Ribosomal protein L31e, conserved site / Ribosomal protein L6, alpha-beta domain / Ribosomal protein L23 / Ribosomal protein S17, conserved site / Ribosomal protein L14P, conserved site / Ubiquitin / Ribosomal protein L3, conserved site / Ribosomal protein L15e, conserved site / WD40 repeat, conserved site / Ribosomal protein L7Ae/L8/Nhp2 family / Ribosomal protein S8e, conserved site / Ribosomal protein S6e, conserved site / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3, conserved site / Ribosomal protein S19e, conserved site / Ribosomal protein S17e, conserved site / Ribosomal protein S14, conserved site / Ribosomal protein S13, conserved site / Ribosomal protein L37e, conserved site / Ribosomal protein L35Ae, conserved site / Ribosomal protein L32e, conserved site / Ribosomal protein L22/L17, conserved site / Ribosomal protein L11, conserved site / Ribosomal protein L5, conserved site / Ribosomal protein L13e, conserved site / Ribosomal protein L31e domain superfamily / 50S ribosomal protein L30e-like / Ribosomal L28e/Mak16 / Ribosomal protein S28e conserved site / Ribosomal protein S17, archaeal/eukaryotic / 40S ribosomal protein S1/3, eukaryotes / 30s ribosomal protein S13, C-terminal / P-loop containing nucleoside triphosphate hydrolase / Signal recognition particle subunit SRP68 / 60S ribosomal protein L4, C-terminal domain / Ribosomal protein L5 eukaryotic/L18 archaeal, C-terminal / Ribosomal protein L15e core domain superfamily / Ribosomal protein S7 domain / Ribosomal protein L2, archaeal-type / Ribosomal protein L39e domain superfamily / Ribosomal protein S19, superfamily / Ribosomal protein S19 conserved site / Ribosomal protein L4 domain superfamily / Ribosomal protein L24e, conserved site / Ribosomal protein L30, central domain, archaeal type / Ribosomal protein S15P / Ribosomal protein L30e, conserved site / Signal recognition particle, SRP54 subunit / Ribosomal protein L5 domain superfamily / Ribosomal protein S4/S9 / Ribosomal protein L2, conserved site / Ribosomal protein L2, C-terminal / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein L18e, conserved site / Ribosomal protein L18e/L15P / Ribosomal protein L21e, conserved site / Ribosomal protein L10e, conserved site / 60S acidic ribosomal protein P0 / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L41 / Ribosomal protein S30 / Signal recognition particle, SRP54 subunit, eukaryotic / Ribosomal protein S12/S23 / Ribosomal protein L7, eukaryotic / Ribosomal protein L24/L26, conserved site / KOW / Ribosomal protein L26/L24, eukaryotic/archaeal / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S19A/S15e / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein L6, N-terminal / Signal recognition particle, SRP9 subunit / Ribosomal protein L5 eukaryotic/L18 archaeal / Plectin/S10, N-terminal / Ribosomal protein S25 / Basic-leucine zipper domain / Signal recognition particle, SRP54 subunit, M-domain / K Homology domain, type 2 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / AAA+ ATPase domain / Signal recognition particle, SRP14 subunit / RNA-binding S4 domain / Ribosomal protein S27a / Ribosomal protein L14e domain / Signal recognition particle, SRP19 subunit / Ribosomal protein L37ae / Ribosomal protein L34Ae
60S ribosomal protein L13 / 60S ribosomal protein L36 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S4 / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S6 ...60S ribosomal protein L13 / 60S ribosomal protein L36 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S4 / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S6 / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S8 / Ribosomal protein S28 / Uncharacterized protein / 40S ribosomal protein S27 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Signal recognition particle subunit SRP68 / Signal recognition particle 54 kDa protein / X-box-binding protein 1 / Signal recognition particle 14 kDa protein / Signal recognition particle 19 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 60S acidic ribosomal protein P0 / Uncharacterized protein / Uncharacterized protein / 60S ribosomal protein L27 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S12 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 60S ribosomal protein L6 / Uncharacterized protein / Uncharacterized protein / Signal recognition particle 9 kDa protein / Ribosomal protein S9 (Predicted) / Ribosomal protein L10 (Predicted) / 60s ribosomal protein l41 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S30 / Uncharacterized protein / Ribosomal protein L15 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S7 / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S3a / Uncharacterized protein / Uncharacterized protein / Ribosomal protein L37
Biological speciesOryctolagus cuniculus (rabbit) / Saccharomyces cerevisiae (baker's yeast) / Homo sapiens (human) / Canis lupus familiaris (dog) / Rabbit (rabbit) / Baker's yeast (baker's yeast) / Dog (dog) / Human (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.7 Å
AuthorsShanmuganathan V / Cheng J / Braunger K / Berninghausen O / Beatrix B / Beckmann R
CitationJournal: Elife / Year: 2019
Title: Structural and mutational analysis of the ribosome-arresting human XBP1u.
Authors: Vivekanandan Shanmuganathan / Nina Schiller / Anastasia Magoulopoulou / Jingdong Cheng / Katharina Braunger / Florian Cymer / Otto Berninghausen / Birgitta Beatrix / Kenji Kohno / Gunnar von Heijne / Roland Beckmann /
Abstract: XBP1u, a central component of the unfolded protein response (UPR), is a mammalian protein containing a functionally critical translational arrest peptide (AP). Here, we present a 3 Å cryo-EM ...XBP1u, a central component of the unfolded protein response (UPR), is a mammalian protein containing a functionally critical translational arrest peptide (AP). Here, we present a 3 Å cryo-EM structure of the stalled human XBP1u AP. It forms a unique turn in the ribosomal exit tunnel proximal to the peptidyl transferase center where it causes a subtle distortion, thereby explaining the temporary translational arrest induced by XBP1u. During ribosomal pausing the hydrophobic region 2 (HR2) of XBP1u is recognized by SRP, but fails to efficiently gate the Sec61 translocon. An exhaustive mutagenesis scan of the XBP1u AP revealed that only 8 out of 20 mutagenized positions are optimal; in the remaining 12 positions, we identify 55 different mutations increase the level of translational arrest. Thus, the wildtype XBP1u AP induces only an intermediate level of translational arrest, allowing efficient targeting by SRP without activating the Sec61 channel.
Validation ReportPDB-ID: 6r6g

SummaryFull reportAbout validation report
DateDeposition: Mar 27, 2019 / Header (metadata) release: Jul 10, 2019 / Map release: Jul 10, 2019 / Update: Jul 10, 2019

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: : PDB-6r6g
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_4735.map.gz / Format: CCP4 / Size: 236.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 396 pix.
= 429.264 Å
1.08 Å/pix.
x 396 pix.
= 429.264 Å
1.08 Å/pix.
x 396 pix.
= 429.264 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.084 Å
Density
Contour LevelBy AUTHOR: 0.08 / Movie #1: 0.08
Minimum - Maximum-0.45232785 - 0.6131792
Average (Standard dev.)0.001940717 (±0.025703479)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions396396396
Spacing396396396
CellA=B=C: 429.264 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0841.0841.084
M x/y/z396396396
origin x/y/z0.0000.0000.000
length x/y/z429.264429.264429.264
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS396396396
D min/max/mean-0.4520.6130.002

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Supplemental data

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Sample components

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Entire Structure of XBP1u-paused ribosome nascent chain complex with SRP.

EntireName: Structure of XBP1u-paused ribosome nascent chain complex with SRP.
Number of components: 101

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Component #1: protein, Structure of XBP1u-paused ribosome nascent chain complex...

ProteinName: Structure of XBP1u-paused ribosome nascent chain complex with SRP.
Recombinant expression: No

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Component #2: protein, Structure of XBP1u-paused nascent chain complex with SRP.

ProteinName: Structure of XBP1u-paused nascent chain complex with SRP.
Details: ribosome / Recombinant expression: No
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #3: protein, P- and E- site tRNA

ProteinName: P- and E- site tRNA / Recombinant expression: No
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)

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Component #4: protein, X-box-binding protein 1

ProteinName: X-box-binding protein 1 / Details: Pausing sequence of XBP1u / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Oryctolagus cuniculus (rabbit)

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Component #5: protein, mRNA encoding XBP1u

ProteinName: mRNA encoding XBP1u / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: synthetic construct (others)

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Component #6: protein, SRP

ProteinName: SRP / Recombinant expression: No
SourceSpecies: Canis lupus familiaris (dog)

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Component #7: protein, Signal sequence (HR2)

ProteinName: Signal sequence (HR2) / Recombinant expression: No
SourceSpecies: Homo sapiens (human)

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Component #8: protein, eS31

ProteinName: eS31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.98644 kDa
SourceSpecies: Rabbit (rabbit)

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Component #9: protein, X-box-binding protein 1

ProteinName: X-box-binding protein 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 2.89538 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Oryctolagus cuniculus (rabbit)

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Component #10: nucleic-acid, P-tRNA

nucleic acidName: P-tRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GCUUCCGUAG UGCAGCGGCA GCACGUUAGG CUCAUAAUCU AAAGGUCCUU GGUUCGAAAC CAUCCGGGGG CACCA
MassTheoretical: 24.10833 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #11: nucleic-acid, E-tRNA

nucleic acidName: E-tRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GCCCGGAUAG CUCAGCUGUA GAGCAUCAGA CUUAAAAUCU GAGGGUCCAG GGUUCAAGUC CCUGUUCGGG CGCCA
MassTheoretical: 24.148355 kDa
SourceSpecies: Baker's yeast (baker's yeast)

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Component #12: nucleic-acid, mRNA

nucleic acidName: mRNAMessenger RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
UUAAUG
MassTheoretical: 1.877157 kDa
SourceSpecies: Homo sapiens (human)

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Component #13: nucleic-acid, 28S ribosomal RNA

nucleic acidName: 28S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAACG GCGAGUGAAC AGGGAAGAGC CCAGCGCCGA AUCCCCGCCC CGCGGCGGGG CGCGGGAAAU GUGGCGUACG GAAGACCCAC UCCCCGGCGC CGGGGGGCCC AAGUCCUUCU GAUCGAGGCC CAGCCCGUGG ACGGUGUGAG GCCGGUAGCG GCCCCCGGCG CGCCGGGCCC GGGUCUUCCC GGAGUCGGGU UGCUUGGGAA UGCAGCCCAA AGCGGGUGGU AAACUCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA GAGAGAGUUC AAGAGGGCGU GAAACCGUUA AGAGGUAAAC GGGUGGGGUC CGCGCAGUCC GCCCGGAGGA UUCAACCCGG CGGCGCGGUC CGGCCGUGCC GGCGGGGCGG AUCUCCCGCU CCCGUCGGCG GGGGACCGCC CCCCGGCCGG CGACCGGCCG CCGCCGGGCG CAUUUCCACC GCGGCGGUGC GCCGCGACCG GCUCCGGGAC GGCUGGGAAG GCCCCGGCGG GGAAGGUGGC CCGGGGGCCC CGGGUGUUAC AGCCCCGCCC CGGCAGCAGC AGCUCGCCGA AUCCCGGGGC CGAGGGAGCG AGACCCGUGC GCCGCGCUCU CCCCCCUCGG GGGGGCCGGG CCACCCCUCC CACGGCGCGA CCGCUGGGGC GGACUGUCCA GUGCGCCCCG GGCGGGUCGC GCCGUCGGGC CCGGGGGGCC ACGCGUCCCC CGCGAGCGCA CGGGGUCGGC GGCGAUGUCG GCUACCCACC CGACCCGUCU UGAAACACGG ACCAAGGAGU CUAACACGUG CGCGAGUCGG GGGCUACGAA AGCCGCCGUG GCGCAAUGAA GGUGAAGGCC GGCGCGCGCG CCGCCGGCCG AGGUGGGAUC CCGAGGCCUU CCAGUCCGCC GAGGGCGCAC CACCGGCCCG UCUCGCCCGC CGCGCCGGGG AGGUGGAGCA CGAGCGCACG UGUUAGGACC CGAAAGAUGG UGAACUAUGC CUGGGCAGGG CGAAGCCAGA GGAAACUCUG GUGGAGGUCC GUAGCGGUCC UGACGUGCGA AUCGGUCGUC CGACCUGGGU AUAGGGGCGA AAGACUAAUC GAACCAUCUA GUAGCUGGUU CCCUCCAAAG UUUCCCUCAG GAUAGCUGGC GCUCUCCGCA GUUUUAUCCG GUAAAGCGAA UGAUUAGAGG UCUUGGGGCC GAAACGAUCU CAACCUAUUC UCAAACUUUA AAUGGGUAAG AAGCCCGGCU CGCUGGUGUG GAGCCGGGCG UGGAAUGCGA GUGCCUAGUG GGCCACUUUU GGUAAGCAGA ACUGGCGCUG CGGGAUGAAC CGAACGCCGG GUUAAGGCGC CCGAUGCCGA CGCUCAUCAG ACCCCAGAAA AGGUGUUGGU UGAUAUAGAC AGCAGGAUGG UGGCCAUGGA AGUCGGAAUC CGCUAAGGAG UGUGUAACAA CUCACCUGCC GAAUCAACUA GCCCUGAAAA UGGAUGGCGC UGGAGCGUCG GGCCCAUACC CGGCCGUCGC UGGCAGUCGG CAGAGCGAGA AAGAGAGUGG CGCGGACGCU ACGCCGCGAC GAGUAGGAGG GCCGCUGCGG UGAGCCUUGA AGCCUAGGGC GCGGGCCCGG GUGGAGCCGC CGCAGGUGCA GAUCUUGGUG GUAGUAGCAA AUAUUCAAAC GAGAACUUUG AAGGCCGAAG UGGAGAAGGG UUCCAUGUGA ACAGCAGUUG AACAUGGGUC AGUCGGUCCU GAGAGAUGGG CGAGCGCCGU UCCGAAGGGA CGGGCGAUGG CCUCCGUUGC CCUCAGCCGA UCGAAAGGGA GUCGGGUUCA GAUCCCCGAA UCCGGAGUGG CGGAGAUGGG CGCCGCGAGG CGUCCAGUGC GGUAACGCGA CCGAUCCCGG AGAAGCCGGC GGGAGCCCCG GGGAGAGUUC UCUUUUCUUU GUGAAGGGCA GGGCGCCCUG GAAUGGGUUC GCCCCGAGAG AGGGGCCCGU GCCUUGGAAA GCGUCGCGGU UCCGGCGGCG UCCGGUGAGC UCUCGCUGGC CCUUGAAAAU CCGGGGGAGA GGGUGUAAAU CUCGCGCCGG GCCGUACCCA UAUCCGCAGC AGGUCUCCAA GGUGAACAGC CUCUGGCAUG UUGGAACAAU GUAGGUAAGG GAAGUCGGCA AGCCGGAUCC GUAACUUCGG GAUAAGGAUU GGCUCUAAGG GCUGGCAGCC GACUUAGAAC UGGUGCGGAC CAGGGGAAUC CGACUGUUUA AUUAAAACAA AGCAUCGCGA AGGCCCGCGG CGGGUGUUGA CGCGAUGUGA UUUCUGCCCA GUGCUCUGAA UGUCAAAGUG AAGAAAUUCA AUGAAGCGCG GGUAAACGGC GGGAGUAACU AUGACUCUCU UAAGGUAGCC AAAUGCCUCG UCAUCUAAUU AGUGACGCGC AUGAAUGGAU GAACGAGAUU CCCACUGUCC CUACCUACUA UCCAGCGAAA CCACAGCCAA GGGAACGGGC UUGGCGGAAU CAGCGGGGAA AGAAGACCCU GUUGAGCUUG ACUCUAGUCU GGCACGGUGA AGAGACGUUU UUUCACUGAC CCGGUGAGGC GGGGGGGCGA GCCGGGCUCU CGCUUCUGGC GCCAAGCGCC CGGCCGGCCG GGCGCGACCC GCUCCGGGGA CAGUGCCAGG UGGGGAGUUU GACUGGGGCG GUACACCUGU CAAACGGUAA CGCAGGUGUC CUAAGGCGAG CUCAGGGAGG ACAGAAACCU CCCGUGGAGC AGAAGGGCAA AAGCUCGCUU GAUCUUGAUU UUCAGUACGA AUACAGACCG UGAAAGCGGG GCCUCACGAU CCUUCUGACC UUUUGGGUUU UAAGCAGGAG GUGUCAGAAA AGUUACCACA GGGAUAACUG GCUUGUGGCG GCCAAGCGUU CAUAGCGACG UCGCUUUUUG AUCCUUCGAU GUCGGCUCUU CCUAUCAUUG UGAAGCAGAA UUCACCAAGC GUUGGAUUGU UCACCCACUA AUAGGGAACG UGAGCUGGGU UUAGACCGUC GUGAGACAGG UUAGUUUUAC CCUACUGAUG AUGUGUUGUU GCCAUGGUAA UCCUGCUCAG UACGAGAGGA ACCGCAGGUU CAGACAUUUG GUGUAUGUGC UUGGCUGAGG AGCCAAUGGG GCGAAGCUAC CAUCUGUGGG AUUAUGACUG AACGCCUCUA AGUCAGAAUC CCGCCCAGGC GGAACGAUAG CGCCGGAGCC UCGGUUGGCC UCGGAUAGCC GGUCCCCCGC CGGGGUCCGG UGCGGAGAGC CCUUCGUCCC GGGAAACGGG GCGGCCGGAA AGGGGGCCGC CCUCUCGCCC GUCACGCACC GCACGUUCGU GGGGAACCUG GCGCUAAACC AUUCGUAGAC GACCUGCUUC UGGGUCGGGG UUUCGUACGU AGCAGAGCAG CUGCUGCGAU CUAUUGAAAG UCAGCCCUCG ACACAAGGGU UUGU
MassTheoretical: 1.148420625 MDa
SourceSpecies: Rabbit (rabbit)

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Component #14: protein, ribosomal protein RACK1

ProteinName: ribosomal protein RACK1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.669113 kDa
SourceSpecies: Rabbit (rabbit)

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Component #15: nucleic-acid, 5S ribosomal RNA

nucleic acidName: 5S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAGA CCGCCUGGGA AUACCGGGUG CUGUAGGCUU
MassTheoretical: 38.691914 kDa
SourceSpecies: Rabbit (rabbit)

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Component #16: nucleic-acid, 5.8S ribosomal RNA

nucleic acidName: 5.8S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGU UGAUCAUCGA CACUUCGAAC GCACUUGCGG CCCCGGGUUC CUCCCGGGGC UACGCCUGUC UGAGCGUCGC U
MassTheoretical: 48.545672 kDa
SourceSpecies: Rabbit (rabbit)

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Component #17: protein, uS14

ProteinName: uS14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.559625 kDa
SourceSpecies: Rabbit (rabbit)

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Component #18: protein, uL2

ProteinName: uL2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.998613 kDa
SourceSpecies: Rabbit (rabbit)

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Component #19: protein, 40S ribosomal protein S30

ProteinName: 40S ribosomal protein S30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.317539 kDa
SourceSpecies: Rabbit (rabbit)

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Component #20: protein, uL3

ProteinName: uL3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 45.119812 kDa
SourceSpecies: Rabbit (rabbit)

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Component #21: protein, 40S ribosomal protein S7

ProteinName: 40S ribosomal protein S7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.629309 kDa
SourceSpecies: Rabbit (rabbit)

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Component #22: protein, uL4

ProteinName: uL4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 41.101414 kDa
SourceSpecies: Rabbit (rabbit)

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Component #23: protein, 40S ribosomal protein S8

ProteinName: 40S ribosomal protein S8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.003012 kDa
SourceSpecies: Rabbit (rabbit)

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Component #24: protein, Ribosomal protein S9 (Predicted)

ProteinName: Ribosomal protein S9 (Predicted)Ribosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.649633 kDa
SourceSpecies: Rabbit (rabbit)

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Component #25: protein, 60S ribosomal protein L5

ProteinName: 60S ribosomal protein L5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.077391 kDa
SourceSpecies: Rabbit (rabbit)

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Component #26: protein, Ribosomal protein S11

ProteinName: Ribosomal protein S11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.586766 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #27: protein, 60S ribosomal protein L6

ProteinName: 60S ribosomal protein L6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.818293 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #28: protein, Ribosomal protein S28

ProteinName: Ribosomal protein S28 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.007069 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #29: protein, uL30

ProteinName: uL30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.658754 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #30: protein, Ribosomal protein S20

ProteinName: Ribosomal protein S20 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.395489 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #31: protein, eL8

ProteinName: eL8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.351377 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #32: protein, uL6

ProteinName: uL6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.627215 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #33: protein, 40S ribosomal protein S21

ProteinName: 40S ribosomal protein S21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.124389 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #34: protein, Ribosomal protein L10 (Predicted)

ProteinName: Ribosomal protein L10 (Predicted)Ribosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.511861 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #35: protein, uS13

ProteinName: uS13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.898648 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #36: protein, 40S ribosomal protein S27

ProteinName: 40S ribosomal protein S27 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.34899 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #37: protein, Ribosomal protein L11

ProteinName: Ribosomal protein L11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.399498 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #38: nucleic-acid, 18S ribosomal RNA

nucleic acidName: 18S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGCG AAUGGCUCAU UAAAUCAGUU AUGGUUCCUU UGGUCGCUCG UACUUGGAUA ACUGUGGUAA UUCUAGAGCU AAUACAUGCC GACGGGCGCU GACCCCCUUC GCGGGGGGGA UGCGUGCAUU UAUCAGUGGU GACUCUAGAU AACCUCGGGC CGAUCGCACG CCCGGCGGCG ACGACCCAUU CGAACGUCUG CCCUAUCAAC UUUCGAUGGU AGUCGCCGUG CCUACCAUGG UGACCACGGG UGACGGGGAA UCAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAACGGCUA CCACAUCCAA GGAAGGCAGC AGGCGCGCAA AUUACCCACU CCCGACCCGG GGAGGUAGUG ACGAAAAAUA ACAAUACAGG ACUCUUUCGA GGCCCUGUAA UUGGAAUGAG UCCACUUUAA AUCCUUUAAC GAGGAUCCAU UGGAGGGCAA GUCUGGUGCC AGCAGCCGCG GUAAUUCCAG CUCCAAUAGC GUAUAUUAAA GUUGCUGCAG UUAAAAAGCU CGUAGUUGGA UCUUGGGAGC GGCCGUCCCC UGCUCGGCGC CGGCCCGAAG CGUUUACUUU GAAAAAAUUA GAGUGUUCAA AGCAGGCCGC CUGGAUACCG CAGCUAGGAA UAAUGGAAUA GGACCGCGGU UCUAUUUUGU UGGUUUUCGG AACUGAGGCC AUGAUUAAGA GGGACGGCCG GGGGCAUUCG UAUUGCGCCG CUAGAGGUGA AAUUCUUGGA CCGGCGCAAG ACGGACCAGA GCGAAAGCAU UUGCCAAGAA UGUUUUCAUU AAUCAAGAAC GAAAGUCGGA GGUUCGAAGA CGAUCAGAUA CCGUCGUAGU UCCGACCAUA AACGAUGCCG ACUGGCGAUG CGGCGGCGUU AUUCCCAUGA CCCGCCGGGC AGCUUCCGGG AAACCAAAGU CUUUGGGUUC CGGGGGGAGU AUGGUUGCAA AGCUGAAACU UAAAGGAAUU GACGGAAGGG CACCACCAGG AGUGGAGCCU GCGGCUUAAU UUGACUCAAC ACGGGAAACC UCACCCGGCC CGGACACGGA CAGGAUUGAC AGAUUGAUAG CUCUUUCUCG AUUCCGUGGG UGGUGGUGCA UGGCCGUUCU UAGUUGGUGG AGCGAUUUGU CUGGUUAAUU CCGAUAACGA ACGAGACUCU GGCAUGCUAA CUAGUUACGC GACCCCGGUC GGCGUAACUU CUUAGAGGGA CAAGUGGCGU UCAGCCACCC GAGAUUGAGC AAUAACAGGU CUGUGAUGCC CUUAGAUGUC CGGGGCUGCA CGCGCGCUAC ACUGACUGGC UCAGCGUGUG CCUACCCUAC GCCGGCAGGC GCGGGUAACC CGUUGAACCC CAUUCGUGAU GGGGAUCGGG GAUUGCAAUU AUUCCCCAUG AACGAGGAAU UCCCAGUAAG UGCGGGUCAU AAGCUUGCGU UGAUUAAGUC CCUGCCCUUU GUACACACCG CCCGUCGCUA CUACCGAUUG GAUGGUUUAG UGAGGCCCUC GGAUCGGCCC CGCCGGGGUG CCCUGGCGGA GCGCUGAGAA GACGGUCGAA CUUGACUAUC UAGAGGAAGU AAAAGUCGUA ACAAGGUUUC CGUAGGUGAA CCUGCGGAAG GAUCAUUA
MassTheoretical: 548.040188 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #39: protein, eS17

ProteinName: eS17 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.250714 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #40: protein, 60S ribosomal protein L13

ProteinName: 60S ribosomal protein L13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.200525 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #41: protein, 40S ribosomal protein S26

ProteinName: 40S ribosomal protein S26 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.645794 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #42: protein, Ribosomal protein L14

ProteinName: Ribosomal protein L14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.217355 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #43: protein, 40S ribosomal protein S14

ProteinName: 40S ribosomal protein S14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.544659 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #44: protein, Ribosomal protein L15

ProteinName: Ribosomal protein L15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.076088 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #45: protein, 40S ribosomal protein S24

ProteinName: 40S ribosomal protein S24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.432125 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #46: protein, 60S ribosomal protein L13a

ProteinName: 60S ribosomal protein L13a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.144859 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #47: protein, ribosomal protein eS25

ProteinName: ribosomal protein eS25 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.526119 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #48: protein, uL22

ProteinName: uL22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.758678 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #49: protein, eS19

ProteinName: eS19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.611003 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #50: protein, eL18

ProteinName: eL18 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.568492 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #51: protein, ribosomal protein uS15

ProteinName: ribosomal protein uS15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.057113 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #52: protein, 60S ribosomal protein L19

ProteinName: 60S ribosomal protein L19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.613033 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #53: protein, 40S ribosomal protein S12

ProteinName: 40S ribosomal protein S12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.048244 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #54: protein, eL20

ProteinName: eL20 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.827561 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #55: protein, eS10

ProteinName: eS10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.529682 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #56: protein, eL21

ProteinName: eL21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.478791 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #57: protein, Ribosomal protein S15a

ProteinName: Ribosomal protein S15aRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.734357 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #58: protein, Ribosomal protein S16

ProteinName: Ribosomal protein S16 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.032804 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #59: protein, 60S ribosomal protein L22

ProteinName: 60S ribosomal protein L22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.495275 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #60: protein, Ribosomal protein S23

ProteinName: Ribosomal protein S23 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.626392 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #61: protein, eL14

ProteinName: eL14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.972425 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #62: protein, Ribosomal protein L24

ProteinName: Ribosomal protein L24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.131536 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #63: protein, uS19

ProteinName: uS19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.246854 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #64: protein, uL23

ProteinName: uL23 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.727181 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #65: protein, Ribosomal protein L26

ProteinName: Ribosomal protein L26 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.891787 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #66: protein, 60S ribosomal protein L27

ProteinName: 60S ribosomal protein L27 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.704635 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #67: protein, uL15

ProteinName: uL15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.489363 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #68: protein, eL29

ProteinName: eL29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.401047 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #69: protein, eL30

ProteinName: eL30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.914756 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #70: protein, eL31

ProteinName: eL31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.635694 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #71: protein, eL32

ProteinName: eL32CD59 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.022021 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #72: protein, eL33

ProteinName: eL33 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.449612 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #73: protein, eL34

ProteinName: eL34 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.994621 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #74: protein, uL29

ProteinName: uL29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.435403 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #75: protein, 60S ribosomal protein L36

ProteinName: 60S ribosomal protein L36 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.888371 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #76: protein, Ribosomal protein L37

ProteinName: Ribosomal protein L37 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.090891 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #77: protein, 60S ribosomal protein L38

ProteinName: 60S ribosomal protein L38 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.107752 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #78: protein, eL39

ProteinName: eL39 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.324579 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #79: protein, 60S ribosomal protein L40

ProteinName: 60S ribosomal protein L40 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.199574 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #80: protein, 60s ribosomal protein l41

ProteinName: 60s ribosomal protein l41 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 3.473451 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #81: protein, eL42

ProteinName: eL42 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.198603 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #82: protein, ribosomal protein eL43

ProteinName: ribosomal protein eL43 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.168153 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #83: protein, uS2

ProteinName: uS2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.361861 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #84: protein, eL28

ProteinName: eL28 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.220686 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #85: protein, 60S acidic ribosomal protein P0

ProteinName: 60S acidic ribosomal protein P0 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.521062 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #86: protein, uL11

ProteinName: uL11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.561117 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #87: protein, 40S ribosomal protein S3a

ProteinName: 40S ribosomal protein S3a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.759145 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #88: protein, uS5

ProteinName: uS5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.441846 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #89: protein, Ribosomal protein S3

ProteinName: Ribosomal protein S3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.215586 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #90: protein, 40S ribosomal protein S4

ProteinName: 40S ribosomal protein S4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.523674 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #91: protein, Ribosomal protein S5

ProteinName: Ribosomal protein S5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.525941 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #92: protein, 40S ribosomal protein S6

ProteinName: 40S ribosomal protein S6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.471535 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #93: protein, Signal recognition particle 54 kDa protein

ProteinName: Signal recognition particle 54 kDa protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 47.844172 kDa
SourceSpecies: Dog (dog)

+
Component #94: nucleic-acid, 7S RNA

nucleic acidName: 7S RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GCCGGGCGCG GUGGCGCGCG CCUGUAGUCC CAGCUACUCG GGAGGCUGAG GCUGGAGGAU CGCUUGACUA AGUUCGGCAU CAAUAUGGUG ACCUCCCGGG AGCGGGGGAC CACCAGGUUG CCUAAGGAGG GGUGAACCGG CCCAGGUCGG AAACGGAGCA GGUCAAAACU CCCGUGCUGA UCAGUAGUUU AGCGAGACCC CGUCUC
MassTheoretical: 66.779688 kDa
SourceSpecies: Dog (dog)

+
Component #95: protein, Signal recognition particle subunit SRP19

ProteinName: Signal recognition particle subunit SRP19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.159153 kDa
SourceSpecies: Dog (dog)

+
Component #96: protein, Signal recognition particle subunit SRP68

ProteinName: Signal recognition particle subunit SRP68 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.220695 kDa
SourceSpecies: Dog (dog)

+
Component #97: protein, Signal recognition particle 9 kDa protein

ProteinName: Signal recognition particle 9 kDa protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.705137 kDa
SourceSpecies: Dog (dog)

+
Component #98: protein, Signal recognition particle 14 kDa protein

ProteinName: Signal recognition particle 14 kDa protein / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.651371 kDa
SourceSpecies: Dog (dog)

+
Component #99: protein, Signal sequence (HR2)

ProteinName: Signal sequence (HR2) / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 1.375909 kDa
SourceSpecies: Human (human)

+
Component #100: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 299 / Recombinant expression: No
MassTheoretical: 2.430505 MDa

+
Component #101: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 5 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

-
Experimental details

-
Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 28 e/Å2 / Illumination mode: SPOT SCAN
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: FEI FALCON II (4k x 4k)

-
Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 24875
3D reconstructionSoftware: RELION / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Euler angles: RELION
FSC plot (resolution estimation)

-
Atomic model buiding

Modeling #1Refinement protocol: rigid body
Output model

+
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