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- EMDB-4737: Structure of XBP1u-paused ribosome nascent chain complex (rotated... -

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Basic information

Entry
Database: EMDB / ID: EMD-4737
TitleStructure of XBP1u-paused ribosome nascent chain complex (rotated state)
Map data
SampleStructure of XBP1u-paused ribosome nascent chain complex (rotated state).:
A/P- and P/E- site tRNA / (X-box-binding protein ...) x 2 / mRNAMessenger RNA / (nucleic-acidNucleic acid) x 7 / uL2 / uL3 / uL4 / (60S ribosomal protein ...) x 7 / uL30 / eL8 ...A/P- and P/E- site tRNA / (X-box-binding protein ...) x 2 / mRNAMessenger RNA / (nucleic-acidNucleic acid) x 7 / uL2 / uL3 / uL4 / (60S ribosomal protein ...) x 7 / uL30 / eL8 / uL6 / (Ribosomal protein ...) x 21 / uL5 / uL13 / uL22 / eL18 / eL19 / eL20 / eL21 / uL14 / uL23 / uL15 / eL30 / eL31 / eL32CD59 / eL33 / eL34 / uL29 / eL38 / eL40 / eL42 / eL28 / 60S acidic ribosomal protein P0 / uS2 / (40S ribosomal protein ...) x 8 / uS5 / uS11 / eS17 / eS21 / eS24 / eS26 / eS10 / uS14 / uS13 / eS19 / uS10 / S29 / eS31 / (ligand) x 2
Function / homology
Function and homology information


ATF6 (ATF6-alpha) activates chaperone genes / IRE1alpha activates chaperones / XBP1(S) activates chaperone genes / epithelial cell maturation involved in salivary gland development / positive regulation of plasma cell differentiation / positive regulation of phospholipid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter / positive regulation of histone methylation / sterol homeostasis / positive regulation of protein acetylation / positive regulation of vascular wound healing ...ATF6 (ATF6-alpha) activates chaperone genes / IRE1alpha activates chaperones / XBP1(S) activates chaperone genes / epithelial cell maturation involved in salivary gland development / positive regulation of plasma cell differentiation / positive regulation of phospholipid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter / positive regulation of histone methylation / sterol homeostasis / positive regulation of protein acetylation / positive regulation of vascular wound healing / organelle organization / negative regulation of pathway-restricted SMAD protein phosphorylation / positive regulation of ER-associated ubiquitin-dependent protein catabolic process / response to insulin-like growth factor stimulus / positive regulation of immunoglobulin production / positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response / ATF6-mediated unfolded protein response / positive regulation of lactation / exocrine pancreas development / enhancer sequence-specific DNA binding / positive regulation of MHC class II biosynthetic process / negative regulation of myotube differentiation / cellular response to fructose stimulus / cellular triglyceride homeostasis / positive regulation of hepatocyte proliferation / negative regulation of protein neddylation / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / regulation of translation involved in cellular response to UV / oxidized pyrimidine DNA binding / response to TNF agonist / positive regulation of base-excision repair / positive regulation of DNA N-glycosylase activity / negative regulation of DNA repair / positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis / positive regulation of immunoglobulin secretion / negative regulation of endoplasmic reticulum unfolded protein response / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / mammalian oogenesis stage / NF-kappaB complex / cellular response to nutrient / ubiquitin ligase inhibitor activity / negative regulation of RNA splicing / endothelial cell proliferation / positive regulation of TOR signaling / positive regulation of T cell differentiation / positive regulation of endothelial cell apoptotic process / positive regulation of endodeoxyribonuclease activity / activation-induced cell death of T cells / positive regulation of endoplasmic reticulum unfolded protein response / protein kinase A binding / cellular response to laminar fluid shear stress / cytosolic ribosome / supercoiled DNA binding / positive regulation of B cell differentiation / ubiquitin-like protein conjugating enzyme binding / positive regulation of vascular associated smooth muscle cell migration / integral component of endoplasmic reticulum membrane / oxidized purine DNA binding / positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress / positive regulation of T cell receptor signaling pathway / muscle organ development / erythrocyte development / positive regulation of activated T cell proliferation / positive regulation of interleukin-6 secretion / adipose tissue development / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / T cell proliferation involved in immune response / cellular response to interleukin-4 / spindle assembly / ribosomal small subunit biogenesis / neuron development / positive regulation of interleukin-2 production / cellular response to peptide hormone stimulus / ribosomal large subunit biogenesis / protein localization to nucleus / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / positive regulation of JUN kinase activity / gastrulation / protein targeting / IRE1-mediated unfolded protein response / cellular response to glucose starvation / positive regulation of microtubule polymerization / phosphatidylinositol 3-kinase signaling / polysome / positive regulation of fat cell differentiation / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / endoplasmic reticulum unfolded protein response / positive regulation of autophagy / polysomal ribosome / cytoplasmic translation / fatty acid homeostasis / Hsp70 protein binding / mitotic cell cycle checkpoint / cellular response to leukemia inhibitory factor / negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway / regulation of autophagy / response to endoplasmic reticulum stress / positive regulation of vascular smooth muscle cell proliferation
Ribosomal protein L22/L17, conserved site / WD40 repeat, conserved site / Ribosomal protein S9, conserved site / Ribosomal protein S5 domain 2-type fold / G-protein beta WD-40 repeat / Ribosomal protein L39e, conserved site / Ribosomal protein L31e, conserved site / Ribosomal protein L6, alpha-beta domain / Ribosomal protein L23 / Ribosomal protein S17, conserved site ...Ribosomal protein L22/L17, conserved site / WD40 repeat, conserved site / Ribosomal protein S9, conserved site / Ribosomal protein S5 domain 2-type fold / G-protein beta WD-40 repeat / Ribosomal protein L39e, conserved site / Ribosomal protein L31e, conserved site / Ribosomal protein L6, alpha-beta domain / Ribosomal protein L23 / Ribosomal protein S17, conserved site / Ribosomal protein L14P, conserved site / Ubiquitin / Ubiquitin conserved site / Ribosomal protein L3, conserved site / Ribosomal protein L7Ae/L8/Nhp2 family / Ribosomal protein L11, C-terminal / Ribosomal protein S8e, conserved site / Ribosomal protein S6e, conserved site / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3, conserved site / Ribosomal protein S19e, conserved site / Ribosomal protein S17e, conserved site / Ribosomal protein S14, conserved site / Ribosomal protein S13, conserved site / Ribosomal protein L37e, conserved site / Ribosomal protein L35Ae, conserved site / Ribosomal protein L32e, conserved site / Ribosomal protein L27e, conserved site / Ribosomal protein L21e, conserved site / Ribosomal protein S7, conserved site / Ribosomal protein L11, N-terminal / Ribosomal protein L10e, conserved site / Ribosomal protein L4 domain superfamily / Ribosomal protein S28e conserved site / Ribosomal protein S17, archaeal/eukaryotic / 40S ribosomal protein S1/3, eukaryotes / 30s ribosomal protein S13, C-terminal / 60S ribosomal protein L4, C-terminal domain / Ribosomal protein L5 eukaryotic/L18 archaeal, C-terminal / Ribosomal protein L15e core domain superfamily / Ribosomal protein S7 domain / Ribosomal protein L2, archaeal-type / Ribosomal protein L39e domain superfamily / Ribosomal protein L31e domain superfamily / Ribosomal protein S19, superfamily / Ribosomal protein L24e, conserved site / Ribosomal protein L11, conserved site / Ribosomal protein L30, central domain, archaeal type / Ribosomal protein S15P / Ribosomal protein L5 domain superfamily / Ribosomal protein S4/S9 / Ribosomal protein L2, conserved site / Ribosomal protein L2, C-terminal / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein L18e, conserved site / Ribosomal protein L18e/L15P / Ribosomal protein S19 conserved site / Ribosomal protein L5, conserved site / Ribosomal protein L15e, conserved site / Ribosomal protein L13e, conserved site / Ribosomal protein L29, conserved site / 50S ribosomal protein L30e-like / Ribosomal protein L6, N-terminal / Ribosomal protein S12/S23 / Ribosomal protein L7, eukaryotic / Ribosomal protein L24/L26, conserved site / KOW / Ribosomal protein L26/L24, eukaryotic/archaeal / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S19A/S15e / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal protein L41 / Plectin/S10, N-terminal / Ribosomal protein S25 / Basic-leucine zipper domain / K Homology domain, type 2 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / RNA-binding S4 domain / Ribosomal protein S27a / Ribosomal protein L14e domain / Ribosomal protein L37ae / Ribosomal protein L29e / Ribosomal protein L28e / Ribosomal protein L22e / Ribosomal protein L6, conserved site-2 / Ribosomal protein S30 / Ribosomal protein L34Ae / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e, N-terminal / Ribosomal protein S4, conserved site / Ribosomal protein L34e, conserved site / Ribosomal protein L30, conserved site / WD40-repeat-containing domain / Ribosomal protein L10e/L16
60S ribosomal protein L29 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 60S ribosomal protein L13 / Uncharacterized protein / 40S ribosomal protein S8 / Uncharacterized protein / 40S ribosomal protein S6 / Uncharacterized protein ...60S ribosomal protein L29 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 60S ribosomal protein L13 / Uncharacterized protein / 40S ribosomal protein S8 / Uncharacterized protein / 40S ribosomal protein S6 / Uncharacterized protein / 40S ribosomal protein S4 / 60S ribosomal protein L36 / Ribosomal protein S28 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 60S ribosomal protein L6 / Uncharacterized protein / 60S ribosomal protein L27 / 40S ribosomal protein S27 / Uncharacterized protein / 60S acidic ribosomal protein P0 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / X-box-binding protein 1 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 60s ribosomal protein l41 / Ribosomal protein L10 (Predicted) / Ribosomal protein S9 (Predicted) / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S12 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S3a / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S7 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Ribosomal protein L15 / Uncharacterized protein / 40S ribosomal protein S30 / Ribosomal protein L37
Biological speciesOryctolagus cuniculus (rabbit) / Saccharomyces cerevisiae (baker's yeast) / Homo sapiens (human) / Rabbit (rabbit)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsShanmuganathan V / Cheng J / Berninghausen O / Beckmann R
CitationJournal: Elife / Year: 2019
Title: Structural and mutational analysis of the ribosome-arresting human XBP1u.
Authors: Vivekanandan Shanmuganathan / Nina Schiller / Anastasia Magoulopoulou / Jingdong Cheng / Katharina Braunger / Florian Cymer / Otto Berninghausen / Birgitta Beatrix / Kenji Kohno / Gunnar von Heijne / Roland Beckmann /
Abstract: XBP1u, a central component of the unfolded protein response (UPR), is a mammalian protein containing a functionally critical translational arrest peptide (AP). Here, we present a 3 Å cryo-EM ...XBP1u, a central component of the unfolded protein response (UPR), is a mammalian protein containing a functionally critical translational arrest peptide (AP). Here, we present a 3 Å cryo-EM structure of the stalled human XBP1u AP. It forms a unique turn in the ribosomal exit tunnel proximal to the peptidyl transferase center where it causes a subtle distortion, thereby explaining the temporary translational arrest induced by XBP1u. During ribosomal pausing the hydrophobic region 2 (HR2) of XBP1u is recognized by SRP, but fails to efficiently gate the Sec61 translocon. An exhaustive mutagenesis scan of the XBP1u AP revealed that only 8 out of 20 mutagenized positions are optimal; in the remaining 12 positions, we identify 55 different mutations increase the level of translational arrest. Thus, the wildtype XBP1u AP induces only an intermediate level of translational arrest, allowing efficient targeting by SRP without activating the Sec61 channel.
Validation ReportPDB-ID: 6r6p

SummaryFull reportAbout validation report
DateDeposition: Mar 27, 2019 / Header (metadata) release: Jul 10, 2019 / Map release: Jul 10, 2019 / Update: Jul 10, 2019

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.08
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  • Surface view colored by height
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: : PDB-6r6p
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_4737.map.gz / Format: CCP4 / Size: 236.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 396 pix.
= 429.264 Å
1.08 Å/pix.
x 396 pix.
= 429.264 Å
1.08 Å/pix.
x 396 pix.
= 429.264 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.084 Å
Density
Contour LevelBy AUTHOR: 0.08 / Movie #1: 0.08
Minimum - Maximum-0.43451777 - 0.74134743
Average (Standard dev.)0.0016621348 (±0.023827193)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions396396396
Spacing396396396
CellA=B=C: 429.264 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0841.0841.084
M x/y/z396396396
origin x/y/z0.0000.0000.000
length x/y/z429.264429.264429.264
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS396396396
D min/max/mean-0.4350.7410.002

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Supplemental data

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Sample components

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Entire Structure of XBP1u-paused ribosome nascent chain complex (rotated...

EntireName: Structure of XBP1u-paused ribosome nascent chain complex (rotated state).
Number of components: 92

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Component #1: protein, Structure of XBP1u-paused ribosome nascent chain complex...

ProteinName: Structure of XBP1u-paused ribosome nascent chain complex (rotated state).
Recombinant expression: No

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Component #2: protein, Structure of XBP1u-paused ribosome nascent chain complex...

ProteinName: Structure of XBP1u-paused ribosome nascent chain complex (rotated state).
Recombinant expression: No
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #3: cellular-component, A/P- and P/E- site tRNA

Cellular-componentName: A/P- and P/E- site tRNA / Recombinant expression: No
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)

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Component #4: cellular-component, X-box-binding protein 1

Cellular-componentName: X-box-binding protein 1 / Details: Pausing sequence of XBP1u / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Oryctolagus cuniculus (rabbit)

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Component #5: cellular-component, mRNA

Cellular-componentName: mRNAMessenger RNA / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: synthetic construct (others)

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Component #6: nucleic-acid, 28S ribosomal RNA

nucleic acidName: 28S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAACG GCGAGUGAAC AGGGAAGAGC CCAGCGCCGA AUCCCCGCCC CGCGGCGGGG CGCGGGACAU GUGGCGUACG GAAGACCCGC UCCCCGGCGC CGCUCGUGGG GGGCCCAAGU CCUUCUGAUC GAGGCCCAGC CCGUGGACGG UGUGAGGCCG GUAGCGGCCC CCGGCGCGCC GGGCCCGGGU CUUCCCGGAG UCGGGUUGCU UGGGAAUGCA GCCCAAAGCG GGUGGUAAAC UCCAUCUAAG GCUAAAUACC GGCACGAGAC CGAUAGUCAA CAAGUACCGU AAGGGAAAGU UGAAAAGAAC UUUGAAGAGA GAGUUCAAGA GGGCGUGAAA CCGUUAAGAG GUAAACGGGU GGGGUCCGCG CAGUCCGCCC GGAGGAUUCA ACCCGGCGGC GGGUCCGGCC GUGUCGGCGG CCCGGCGGAU CUUUCCCGCG CGGGGGACCG UCCCCCGACC GGCGACCGGC CGCCGCCGGG CGCAUUUCCA CCGCGGCGGU GCGCCGCGAC CGGCUCCGGG ACGGCUGGGA AGGCCCGGCG GGGAAGGUGG CUCGGCCGAG UGUUACAGCC CCCCCGGCAG CAGCACUCGC CGAAUCCCGG GGCCGAGGGA GCGAGACCCG UCGCCGCGCU CUCCCCCCUC CCGGCGCCGC CGGGGGGGGC CGGGCCACCC CUCCCACGGC GCGACCGCUC GGGGCGGACU GUCCCCAGUG CGCCCCGGGC GGGUCGCGCC GUCGGGCCCG GGGGAGGGGC CACGCGCGCG UCCCCCGAAG AGGGGGACGG CGGAGCGAGC GCACGGGGUC GGCGGCGACG UCGGCUACCC ACCCGACCCG UCUUGAAACA CGGACCAAGG AGUCUAACAC GUGCGCGAGU CGGGGGCUCG CACGAAAGCC GCCGUGGCGC AAUGAAGGUG AAGGCCGGCG CGCUCGCCGG CCGAGGUGGG AUCCCGAGGC CUCUCCAGUC CGCCGAGGGC GCACCACCGG CCCGUCUCGC CCGCCGCGCC GGGGAGGUGG AGCACGAGCG CACGUGUUAG GACCCGAAAG AUGGUGAACU AUGCCUGGGC AGGGCGAAGC CAGAGGAAAC UCUGGUGGAG GUCCGUAGCG GUCCUGACGU GCAAAUCGGU CGUCCGACCU GGGUAUAGGG GCGAAAGACU AAUCGAACCA UCUAGUAGCU GGUUCCCUCC GAAGUUUCCC UCAGGAUAGC UGGCGCUCUC GCACACGCAG UUUUAUCCGG UAAAGCGAAU GAUUAGAGGU CUUGGGGCCG AAACGAUCUC AACCUAUUCU CAAACUUUAA AUGGGUAAGA AGCCCGGCUC GCUGGCGUGA GCCGGGCGUG GAAUGGAGUG CCUAGUGGGC CACUUUUGGU AAGCAGAACU GGCGCUGCGG GAUGAACCGA ACGCCGGGUU AAGGCGCCCG AUGCCGACGC UCAUCAGACC CCAGAAAAGG UGUUGGUUGA UAUAGACAGC AGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAGGAGUGU GUAACAACUC ACCUGCCGAA UCAACUAGCC CUGAAAAUGG AUGGCGCUGG AGCGUCGGGC CCAUACCCGG CCGUCGCCGG CAGUCGAGAG UGGACGGGAG CGGCGGGGGC GGCGCGCGCG CGCGCCCGCC CCCGGAGCCC CGCGGACGCU ACGCCGCGAC GAGUAGGAGG GCCGCUGCGG UGAGCCUUGA AGCCUAGGGC GCGGGCCCGG GUGGAGCCGC CGCAGGUGCA GAUCUUGGUG GUAGUAGCAA AUAUUCAAAC GAGAACUUUG AAGGCCGAAG UGGAGAAGGG UUCCAUGUGA ACAGCAGUUG AACAUGGGUC AGUCGGUCCU GAGAGAUGGG CGAGCGCCGU UCCGAAGGGA CGGGCGAUGG CCUCCGUUGC CCUCGGCCGA UCGAAAGGGA GUCGGGUUCA GAUCCCCGAA UCCGGAGUGG CGGAGAUGGG CGCCGCGAGG CGUCCAGUGC GGUAACGCGA CCGAUCCCGG AGAAGCCGGC GGGAGCCCCG GGGAGAGUUC UCUUUUCUUU GUGAAGGGCA GGGCGCCCUG GAAUGGGUUC GCCCCGAGAG AGGGGCCCGU GCCUUGGAAA GCGUCGCGGU UCCGGCGGCG UCCGGUGAGC UCUCGCUGGC CCUUGAAAAU CCGGGGGAGA GGGUGUAAAU CUCGCGCCGG GCCGUACCCA UAUCCGCAGC AGGUCUCCAA GGUGAACAGC CUCUGGCAUG UUGGAACAAU GUAGGUAAGG GAAGUCGGCA AGCCGGAUCC GUAACUUCGG GAUAAGGAUU GGCUCUAAGG GCUGGGUCGG UCGUCGGCCG GCGCCUAGCA GCCGACUUAG AACUGGUGCG GACCAGGGGA AUCCGACUGU UUAAUUAAAA CAAAGCAUCG CGAAGGCCCG CGGCGGGUGU UGACGCGAUG UGAUUUCUGC CCAGUGCUCU GAAUGUCAAA GUGAAGAAAU UCAAUGAAGC GCGGGUAAAC GGCGGGAGUA ACUAUGACUC UCUUAAGGUA GCCAAAUGCC UCGUCAUCUA AUUAGUGACG CGCAUGAAUG GAUGAACGAG AUUCCCACUG UCCCUACCUA CUAUCCAGCG AAACCACAGC CAAGGGAACG GGCUUGGCGG AAUCAGCGGG GAAAGAAGAC CCUGUUGAGC UUGACUCUAG UCUGGCACGG UGAAGAGACA UGUUUUUUCA CUGACCCGGU GAGGCGGGGG GGCGAGCCCC GAGGGGCUCU CGCUUCUGGC GCCAAGCGCC CGGCCGCGCG CCGGCCGGGC GCGACCCGCU CCGGGGACAG UGCCAGGUGG GGAGUUUGAC UGGGGCGGUA CACCUGUCAA ACGGUAACGC AGGUGUCCUA AGGCGAGCUC AGGGAGGACA GAAACCUCCC GUGGAGCAGA AGGGCAAAAG CUCGCUUGAU CUUGAUUUUC AGUACGAAUA CAGACCGUGA AAGCGGGGCC UCACGAUCCU UCUGACCUUU UGGGUUUUAA GCAGGAGGUG UCAGAAAAGU UACCACAGGG AUAACUGGCU UGUGGCGGCC AAGCGUUCAU AGCGACGUCG CUUUUUGAUC CUUCGAUGUC GGCUCUUCCU AUCAUUGUGA AGCAGAAUUC ACCAAGCGUU GGAUUGUUCA CCCACUAAUA GGGAACGUGA GCUGGGUUUA GACCGUCGUG AGACAGGUUA GUUUUACCCU ACUGAUGAUG UGUUGUUGCC AUGGUAAUCC UGCUCAGUAC GAGAGGAACC GCAGGUUCAG ACAUUUGGUG UAUGUGCUUG GCUGAGGAGC CAAUGGGGCG AAGCUACCAU CUGUGGGAUU AUGACUGAAC GCCUCUAAGU CAGAAUCCCG CCCAGGCGGA ACGAUACGGC AGCGCCGCGG AGCCUCGGUU GGCCUCGGAU AGCCGGUCCC CCGCGGGGUC CGGUGCGGAG UGCCCUUCGU CCUGGGAAAC GGGGCGCGGC CGGAGAGGCG GCCGCCCCCU CGCCCGUCAC GCACGCCGUU CGUGGGGAAC CUGGCGCUAA ACCAUUCGUA GACGACCUGC UUCUGGGUCG GGGUUUCGUA CGUAGCAGAG CAGCUCCCUC GCUGCGAUCU AUUGAAAGUC AGCCCUCGAC ACAAGGGUUU GU
MassTheoretical: 1.1865795 MDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #7: nucleic-acid, 5S ribosomal RNA

nucleic acidName: 5S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAGA CCGCCUGGGA AUACCGGGUG CUGUAGGCUU
MassTheoretical: 38.691914 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #8: nucleic-acid, 5.8S ribosomal RNA

nucleic acidName: 5.8S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAUC AUCGACACUU CGAACGCACU UGCGGCCCCG GGUUCCUCCC GGGGCUACGC CUGUCUGAGC GUCGCU
MassTheoretical: 50.143648 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #9: protein, uL2

ProteinName: uL2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.570105 kDa
SourceSpecies: Rabbit (rabbit)

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Component #10: protein, uL3

ProteinName: uL3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 45.119812 kDa
SourceSpecies: Rabbit (rabbit)

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Component #11: protein, uL4

ProteinName: uL4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 41.101414 kDa
SourceSpecies: Rabbit (rabbit)

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Component #12: protein, 60S ribosomal protein L5

ProteinName: 60S ribosomal protein L5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.006312 kDa
SourceSpecies: Rabbit (rabbit)

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Component #13: protein, 60S ribosomal protein L6

ProteinName: 60S ribosomal protein L6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 28.529848 kDa
SourceSpecies: Rabbit (rabbit)

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Component #14: protein, uL30

ProteinName: uL30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 26.662787 kDa
SourceSpecies: Rabbit (rabbit)

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Component #15: protein, eL8

ProteinName: eL8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.480555 kDa
SourceSpecies: Rabbit (rabbit)

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Component #16: protein, uL6

ProteinName: uL6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.627215 kDa
SourceSpecies: Rabbit (rabbit)

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Component #17: protein, Ribosomal protein L10 (Predicted)

ProteinName: Ribosomal protein L10 (Predicted)Ribosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.511861 kDa
SourceSpecies: Rabbit (rabbit)

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Component #18: protein, uL5

ProteinName: uL5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.270318 kDa
SourceSpecies: Rabbit (rabbit)

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Component #19: protein, 60S ribosomal protein L13

ProteinName: 60S ribosomal protein L13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.216525 kDa
SourceSpecies: Rabbit (rabbit)

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Component #20: protein, Ribosomal protein L14

ProteinName: Ribosomal protein L14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.217355 kDa
SourceSpecies: Rabbit (rabbit)

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Component #21: protein, Ribosomal protein L15

ProteinName: Ribosomal protein L15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.076088 kDa
SourceSpecies: Rabbit (rabbit)

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Component #22: protein, uL13

ProteinName: uL13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.248924 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #23: protein, uL22

ProteinName: uL22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.758678 kDa
SourceSpecies: Rabbit (rabbit)

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Component #24: protein, eL18

ProteinName: eL18 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.457391 kDa
SourceSpecies: Rabbit (rabbit)

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Component #25: protein, eL19

ProteinName: eL19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.613033 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #26: protein, eL20

ProteinName: eL20 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.696363 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

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Component #27: protein, eL21

ProteinName: eL21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.478791 kDa
SourceSpecies: Rabbit (rabbit)

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Component #28: protein, Ribosomal protein L22

ProteinName: Ribosomal protein L22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.481249 kDa
SourceSpecies: Rabbit (rabbit)

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Component #29: protein, uL14

ProteinName: uL14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.972425 kDa
SourceSpecies: Rabbit (rabbit)

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Component #30: protein, Ribosomal protein L24

ProteinName: Ribosomal protein L24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.512774 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #31: protein, uL23

ProteinName: uL23 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.85636 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #32: protein, Ribosomal protein L26

ProteinName: Ribosomal protein L26 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.891787 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #33: protein, 60S ribosomal protein L27

ProteinName: 60S ribosomal protein L27 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.704635 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #34: protein, uL15

ProteinName: uL15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.489363 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #35: protein, 60S ribosomal protein L29

ProteinName: 60S ribosomal protein L29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.706283 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #36: protein, eL30

ProteinName: eL30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.496355 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #37: protein, eL31

ProteinName: eL31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.635694 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #38: protein, eL32

ProteinName: eL32CD59 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.022021 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #39: protein, eL33

ProteinName: eL33 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.449612 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #40: protein, eL34

ProteinName: eL34 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.994621 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #41: protein, uL29

ProteinName: uL29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.435403 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #42: protein, 60S ribosomal protein L36

ProteinName: 60S ribosomal protein L36 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.888371 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #43: protein, Ribosomal protein L37

ProteinName: Ribosomal protein L37 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.090891 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #44: protein, eL38

ProteinName: eL38 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.107752 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #45: protein, ribosomal protein eL39

ProteinName: ribosomal protein eL39 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.295562 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #46: protein, eL40

ProteinName: eL40 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.199574 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #47: protein, 60s ribosomal protein l41

ProteinName: 60s ribosomal protein l41 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 3.213075 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #48: protein, eL42

ProteinName: eL42 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.198603 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #49: protein, ribosomal protein eL43

ProteinName: ribosomal protein eL43 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.168153 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #50: protein, eL28

ProteinName: eL28 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.319816 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #51: protein, 60S acidic ribosomal protein P0

ProteinName: 60S acidic ribosomal protein P0 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.747293 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #52: protein, Ribosomal protein L12

ProteinName: Ribosomal protein L12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.689461 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #53: protein, X-box-binding protein 1

ProteinName: X-box-binding protein 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 2.89538 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Oryctolagus cuniculus (rabbit)

+
Component #54: nucleic-acid, A/P-tRNA

nucleic acidName: A/P-tRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GCUUCCGUAG UGCAGCGGCU AGCACGUUAG GCUCAUAAUC UAAAGGUCCU UGGUUCGAAA CCAUCCGGGG GCACCA
MassTheoretical: 24.414496 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)

+
Component #55: nucleic-acid, P/E-tRNA

nucleic acidName: P/E-tRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GCCCGGAUAG CUCAGCUGUA GAGCAUCAGA CUUAAAAUCU GAGGGUCCAG GGUUCAAGUC CCUGUUCGGG CGCCA
MassTheoretical: 24.148355 kDa
SourceSpecies: Saccharomyces cerevisiae (baker's yeast)

+
Component #56: nucleic-acid, 18S rRNA

nucleic acidName: 18S rRNA18S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGCG AAUGGCUCAU UAAAUCAGUU AUGGUUCCUU UGGUCGCUCG UACUUGGAUA ACUGUGGUAA UUCUAGAGCU AAUACAUGCC GACGGGCGCU GACCCCCUUC GCGGGGGGGA UGCGUGCAUU UAUCAGUGGU GACUCUAGAU AACCUCGGGC CGAUCGCACG CCCGGCGGCG ACGACCCAUU CGAACGUCUG CCCUAUCAAC UUUCGAUGGU AGUCGCCGUG CCUACCAUGG UGACCACGGG UGACGGGGAA UCAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAACGGCUA CCACAUCCAA GGAAGGCAGC AGGCGCGCAA AUUACCCACU CCCGACCCGG GGAGGUAGUG ACGAAAAAUA ACAAUACAGG ACUCUUUCGA GGCCCUGUAA UUGGAAUGAG UCCACUUUAA AUCCUUUAAC GAGGAUCCAU UGGAGGGCAA GUCUGGUGCC AGCAGCCGCG AUAAUUCCAG CUCCAAUAGC GUAUAUUAAA GUUGCUGCAG UUAAAAAGCU CGUAGUUGGA UCUUGGGAGC GGCCGUCCCC UGCUCGGCGC CGGCCCGAAG CGUUUACUUU GAAAAAAUUA GAGUGUUCAA AGCAGGCCGC CUGGAUACCG CAGCUAGGAA UAAUGGAAUA GGACCGCGGU UCUAUUUUGU UGGUUUUCGG AACUGAGGCC AUGAUUAAGA GGGACGGCCG GGGGCAUUCG UAUUGCGCCG CUAGAGGUGA AAUUCUUGGA CCGGCGCAAG ACGGACCAGA GCGAAAGCAU UUGCCAAGAA UGUUUUCAUU AAUCAAGAAC GAAAGUCGGA GGUUCGAAGA CGAUCAGAUA CCGUCGUAGU UCCGACCAUA AACGAUGCCG ACUGGCGAUG CGGCGGCGUU AUUCCCAUGA CCCGCCGGGC AGCUUCCGGG AAACCAAAGU CUUUGGGUUC CGGGGGGAGU AUGGUUGCAA AGCUGAAACU UAAAGGAAUU GACGGAAGGG CACCACCAGG AGUGGAGCCU GCGGCUUAAU UUGACUCAAC ACGGGAAACC UCACCCGGCC CGGACACGGA CAGGAUUGAC AGAUUGAUAG CUCUUUCUCG AUUCCGUGGG UGGUGGUGCA UGGCCGUUCU UAGUUGGUGG AGCGAUUUGU CUGGUUAAUU CCGAUAACGA ACGAGACUCU GGCAUGCUAA CUAGUUACGC GACCCCGGUC GGCGUAACUU CUUAGAGGGA CAAGUGGCGU UCAGCCACCC GAGAUUGAGC AAUAACAGGU CUGUGAUGCC CUUAGAUGUC CGGGGCUGCA CGCGCGCUAC ACUGACUGGC UCAGCGUGUG CCUACCCUAC GCCGGCAGGC GCGGGUAACC CGUUGAACCC CAUUCGUGAU GGGGAUCGGG GAUUGCAAUU AUUCCCCAUG AACGAGGAAU UCCCAGUAAG UGCGGGUCAU AAGCUUGCGU UGAUUAAGUC CCUGCCCUUU GUACACACCG CCCGUCGCUA CUACCGAUUG GAUGGUUUAG UGAGGCCCUC GGAUCGGCCC CGCCGGGGUG CCCUGGCGGA GCGCUGAGAA GACGGUCGAA CUUGACUAUC UAGAGGAAGU AAAAGUCGUA ACAAGGUUUC CGUAGGUGAA CCUGCGGAAG GAUCAUUA
MassTheoretical: 548.024188 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #57: protein, uS2

ProteinName: uS2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.361861 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #58: protein, 40S ribosomal protein S3a

ProteinName: 40S ribosomal protein S3a / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.759145 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #59: protein, uS5

ProteinName: uS5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.441846 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #60: protein, 40S ribosomal protein S4

ProteinName: 40S ribosomal protein S4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.523674 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #61: protein, 40S ribosomal protein S6

ProteinName: 40S ribosomal protein S6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.471535 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #62: protein, 40S ribosomal protein S7

ProteinName: 40S ribosomal protein S7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.629309 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #63: protein, 40S ribosomal protein S8

ProteinName: 40S ribosomal protein S8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.003012 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #64: protein, Ribosomal protein S9 (Predicted)

ProteinName: Ribosomal protein S9 (Predicted)Ribosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.649633 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #65: protein, Ribosomal protein S11

ProteinName: Ribosomal protein S11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.586766 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #66: protein, ribosomal protein uS15

ProteinName: ribosomal protein uS15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 17.057113 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #67: protein, uS11

ProteinName: uS11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.544659 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #68: protein, Ribosomal protein S16

ProteinName: Ribosomal protein S16 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.032804 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #69: protein, eS17

ProteinName: eS17 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.250714 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #70: protein, eS21

ProteinName: eS21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.124389 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #71: protein, Ribosomal protein S15a

ProteinName: Ribosomal protein S15aRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.734357 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #72: protein, Ribosomal protein S23

ProteinName: Ribosomal protein S23 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.626392 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #73: protein, eS24

ProteinName: eS24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.432125 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #74: protein, eS26

ProteinName: eS26 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.645794 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #75: protein, 40S ribosomal protein S27

ProteinName: 40S ribosomal protein S27 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.34899 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #76: protein, 40S ribosomal protein S30

ProteinName: 40S ribosomal protein S30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.317539 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #77: protein, Ribosomal protein S3

ProteinName: Ribosomal protein S3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.215586 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #78: protein, Ribosomal protein S5

ProteinName: Ribosomal protein S5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.525941 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #79: protein, eS10

ProteinName: eS10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.529682 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #80: protein, 40S ribosomal protein S12

ProteinName: 40S ribosomal protein S12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.048244 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #81: protein, uS14

ProteinName: uS14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.246854 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #82: protein, uS13

ProteinName: uS13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.898648 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #83: protein, eS19

ProteinName: eS19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.611003 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #84: protein, uS10

ProteinName: uS10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.395489 kDa
SourceSpecies: Oryctolagus cuniculus (rabbit)

+
Component #85: protein, ribosomal protein eS25

ProteinName: ribosomal protein eS25 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.526119 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #86: protein, Ribosomal protein S28

ProteinName: Ribosomal protein S28 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.007069 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #87: protein, S29

ProteinName: S29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.559625 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #88: protein, eS31

ProteinName: eS31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.98644 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #89: protein, ribosomal protein RACK1

ProteinName: ribosomal protein RACK1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.669113 kDa
SourceSpecies: Rabbit (rabbit)

+
Component #90: nucleic-acid, mRNA

nucleic acidName: mRNAMessenger RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
CCAUUAAUGA
MassTheoretical: 3.145932 kDa
SourceSpecies: Homo sapiens (human)

+
Component #91: ligand, MAGNESIUM ION

LigandName: MAGNESIUM ION / Number of Copies: 159 / Recombinant expression: No
MassTheoretical: 2.430505 MDa

+
Component #92: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 5 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

-
Experimental details

-
Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 28 e/Å2 / Illumination mode: SPOT SCAN
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: FEI FALCON II (4k x 4k)

-
Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 94923
3D reconstructionSoftware: RELION / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Euler angles: RELION
FSC plot (resolution estimation)

-
Atomic model buiding

Modeling #1Refinement protocol: rigid body
Output model

+
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