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- EMDB-4667: Cryo-EM structure of T7 bacteriophage portal protein, 13mer, clos... -

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Basic information

Entry
Database: EMDB / ID: EMD-4667
TitleCryo-EM structure of T7 bacteriophage portal protein, 13mer, closed valve
Map data
Sample
  • Complex: Bacteriophage packaging protein
    • Protein or peptide: Portal protein
Function / homologyPortal protein, Caudovirales / Head-to-tail connector protein, podovirus-type / Bacteriophage head to tail connecting protein / viral portal complex / symbiont genome ejection through host cell envelope, short tail mechanism / viral DNA genome packaging / Portal protein
Function and homology information
Biological speciesEnterobacteria phage T7 (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.8 Å
AuthorsCuervo A / Fabrega-Ferrer M / Machon C / Fernandez FJ / Perez-Luque R / Pous J / Vega MC / Carrascosa JL / Coll M
Funding support Spain, 7 items
OrganizationGrant numberCountry
Spanish Ministry of Science, Innovation, and UniversitiesBFU 2014-54181 Spain
Spanish Ministry of Science, Innovation, and UniversitiesBFU2017-83720-P Spain
Spanish Ministry of Science, Innovation, and UniversitiesBFU2014-53550-P Spain
Spanish Ministry of Science, Innovation, and UniversitiesSEV-2013-0347 Spain
Spanish Ministry of Science, Innovation, and UniversitiesMDM-2014-0435 Spain
European Commission653706
Spanish Ministry of Science, Innovation, and UniversitiesRYC-2011-09071 Spain
CitationJournal: Nat Commun / Year: 2019
Title: Structures of T7 bacteriophage portal and tail suggest a viral DNA retention and ejection mechanism.
Authors: Ana Cuervo / Montserrat Fàbrega-Ferrer / Cristina Machón / José Javier Conesa / Francisco J Fernández / Rosa Pérez-Luque / Mar Pérez-Ruiz / Joan Pous / M Cristina Vega / José L Carrascosa / Miquel Coll /
Abstract: Double-stranded DNA bacteriophages package their genome at high pressure inside a procapsid through the portal, an oligomeric ring protein located at a unique capsid vertex. Once the DNA has been ...Double-stranded DNA bacteriophages package their genome at high pressure inside a procapsid through the portal, an oligomeric ring protein located at a unique capsid vertex. Once the DNA has been packaged, the tail components assemble on the portal to render the mature infective virion. The tail tightly seals the ejection conduit until infection, when its interaction with the host membrane triggers the opening of the channel and the viral genome is delivered to the host cell. Using high-resolution cryo-electron microscopy and X-ray crystallography, here we describe various structures of the T7 bacteriophage portal and fiber-less tail complex, which suggest a possible mechanism for DNA retention and ejection: a portal closed conformation temporarily retains the genome before the tail is assembled, whereas an open portal is found in the tail. Moreover, a fold including a seven-bladed β-propeller domain is described for the nozzle tail protein.
History
DepositionMar 7, 2019-
Header (metadata) releaseJul 31, 2019-
Map releaseSep 4, 2019-
UpdateSep 4, 2019-
Current statusSep 4, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0934
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by cylindrical radius
  • Surface level: 0.0934
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4667.png

Downloads & links

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Map

FileDownload / File: emd_4667.map.gz / Format: CCP4 / Size: 15.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.37 Å/pix.
x 160 pix.
= 219.2 Å		1.37 Å/pix.
x 160 pix.
= 219.2 Å		1.37 Å/pix.
x 160 pix.
= 219.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.37 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0934 / Movie #1: 0.0934
Minimum - Maximum-0.103963934 - 0.28356498
Average (Standard dev.)0.0030942515 (±0.027984489)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions160160160
Spacing160160160
CellA=B=C: 219.2 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.371.371.37
M x/y/z160160160
origin x/y/z0.0000.0000.000
length x/y/z219.200219.200219.200
α/β/γ90.00090.00090.000
start NX/NY/NZ-39-149-104
NX/NY/NZ201201211
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS160160160
D min/max/mean-0.1040.2840.003

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Supplemental data

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Sample components

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Entire : Bacteriophage packaging protein

EntireName: Bacteriophage packaging protein
Components
  • Complex: Bacteriophage packaging protein
    • Protein or peptide: Portal protein

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Supramolecule #1: Bacteriophage packaging protein

SupramoleculeName: Bacteriophage packaging protein / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Enterobacteria phage T7 (virus)
Recombinant expressionOrganism: Escherichia coli (E. coli) / Recombinant strain: BL21(DE3)

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Macromolecule #1: Portal protein

MacromoleculeName: Portal protein / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: Enterobacteria phage T7 (virus)
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MAEKRTGLAE DGAKSVYERL KNDRAPYETR AQNCAQYTIP SLFPKDSDNA STDYQTPWQA VGARGLNNL ASKLMLALFP MQTWMRLTIS EYEAKQLLSD PDGLAKVDEG LSMVERIIMN Y IESNSYRV TLFEALKQLV VAGNVLLYLP EPEGSNYNPM KLYRLSSYVV ...String:
MAEKRTGLAE DGAKSVYERL KNDRAPYETR AQNCAQYTIP SLFPKDSDNA STDYQTPWQA VGARGLNNL ASKLMLALFP MQTWMRLTIS EYEAKQLLSD PDGLAKVDEG LSMVERIIMN Y IESNSYRV TLFEALKQLV VAGNVLLYLP EPEGSNYNPM KLYRLSSYVV QRDAFGNVLQ MV TRDQIAF GALPEDIRKA VEGQGGEKKA DETIDVYTHI YLDEDSGEYL RYEEVEGMEV QGS DGTYPK EACPYIPIRM VRLDGESYGR SYIEEYLGDL RSLENLQEAI VKMSMISSKV IGLV NPAGI TQPRRLTKAQ TGDFVTGRPE DISFLQLEKQ ADFTVAKAVS DAIEARLSFA FMLNS AVQR TGERVTAEEI RYVASELEDT LGGVYSILSQ ELQLPLVRVL LKQLQATQQI PELPKE AVE PTISTGLEAI GRGQDLDKLE RCVTAWAALA PMRDDPDINL AMIKLRIANA IGIDTSG IL LTEEQKQQKM AQQSMQMGMD NGAAALAQGM AAQATASPEA MAAAADSVGL QPGIAAALEH HHHHH

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 22.5 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Point group: C13 (13 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 5.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 12642
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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