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- EMDB-3581: Structure of 70S ribosome from Lactococcus lactis -

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Basic information

Entry
Database: EMDB / ID: EMD-3581
TitleStructure of 70S ribosome from Lactococcus lactis
Map data
Sample
  • Complex: 70S ribosome
    • RNA: x 3 types
    • Protein or peptide: x 48 types
Keywordsribosome / 70S / lactoccocus lactis / Cryo-EM
Function / homology
Function and homology information


negative regulation of translational elongation / ribosomal small subunit binding / large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit ...negative regulation of translational elongation / ribosomal small subunit binding / large ribosomal subunit / transferase activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / zinc ion binding / cytosol / cytoplasm
Similarity search - Function
Ribosome hibernation promoting factor, long/plastid / Sigma 54 modulation/S30EA ribosomal protein, C-terminal / Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain superfamily / Sigma 54 modulation/S30EA ribosomal protein C terminus / : / Ribosome hibernation promoting factor/RaiA / Ribosome hibernation promotion factor-like / Sigma 54 modulation protein / S30EA ribosomal protein / Ribosomal protein L31 type B / : ...Ribosome hibernation promoting factor, long/plastid / Sigma 54 modulation/S30EA ribosomal protein, C-terminal / Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain superfamily / Sigma 54 modulation/S30EA ribosomal protein C terminus / : / Ribosome hibernation promoting factor/RaiA / Ribosome hibernation promotion factor-like / Sigma 54 modulation protein / S30EA ribosomal protein / Ribosomal protein L31 type B / : / Ribosomal protein S14, type Z / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein S21 / Ribosomal protein L16 signature 1. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / : / Ribosomal protein L17 signature. / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L28/L24 superfamily / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / : / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / : / Ribosomal protein S19, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L36 / Ribosomal protein S4, bacterial-type / Ribosomal protein L36 superfamily / Ribosomal protein L36 / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S14/S29 / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L28/L24 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / : / L28p-like / Ribosomal protein S15, bacterial-type / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein S12, bacterial-type / Ribosomal protein L20 / Ribosomal L32p protein family / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L19 superfamily / Ribosomal protein L21 / Ribosomal protein L32p / Ribosomal protein L17
Similarity search - Domain/homology
Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein bS21 / Small ribosomal subunit protein uS4 / Ribosome hibernation promotion factor / Large ribosomal subunit protein bL31B / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL21 ...Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein bS21 / Small ribosomal subunit protein uS4 / Ribosome hibernation promotion factor / Large ribosomal subunit protein bL31B / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL17 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein bL33C / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS12
Similarity search - Component
Biological speciesLactococcus lactis (lactic acid bacteria) / Lactococcus lactis subsp. cremoris MG1363 (lactic acid bacteria) / Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.6 Å
AuthorsFranken LE / Oostergetel GT
Funding support Netherlands, 2 items
OrganizationGrant numberCountry
NWOTOPGO L.10.060 Netherlands
European Research CouncilERC-2014-ADG Netherlands
CitationJournal: Nat Commun / Year: 2017
Title: A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy.
Authors: Linda E Franken / Gert T Oostergetel / Tjaard Pijning / Pranav Puri / Valentina Arkhipova / Egbert J Boekema / Bert Poolman / Albert Guskov /
Abstract: Bacteria downregulate their ribosomal activity through dimerization of 70S ribosomes, yielding inactive 100S complexes. In Escherichia coli, dimerization is mediated by the hibernation promotion ...Bacteria downregulate their ribosomal activity through dimerization of 70S ribosomes, yielding inactive 100S complexes. In Escherichia coli, dimerization is mediated by the hibernation promotion factor (HPF) and ribosome modulation factor. Here we report the cryo-electron microscopy study on 100S ribosomes from Lactococcus lactis and a dimerization mechanism involving a single protein: HPF. The N-terminal domain of HPF binds at the same site as HPF in Escherichia coli 100S ribosomes. Contrary to ribosome modulation factor, the C-terminal domain of HPF binds exactly at the dimer interface. Furthermore, ribosomes from Lactococcus lactis do not undergo conformational changes in the 30S head domains upon binding of HPF, and the Shine-Dalgarno sequence and mRNA entrance tunnel remain accessible. Ribosome activity is blocked by HPF due to the inhibition of mRNA recognition by the platform binding center. Phylogenetic analysis of HPF proteins suggests that HPF-mediated dimerization is a widespread mechanism of ribosome hibernation in bacteria.When bacteria enter the stationary growth phase, protein translation is suppressed via the dimerization of 70S ribosomes into inactive complexes. Here the authors provide a structural basis for how the dual domain hibernation promotion factor promotes ribosome dimerization and hibernation in bacteria.
History
DepositionJan 26, 2017-
Header (metadata) releaseFeb 15, 2017-
Map releaseOct 11, 2017-
UpdateMay 15, 2024-
Current statusMay 15, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.035
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  • Surface view colored by radius
  • Surface level: 0.035
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  • Surface view with fitted model
  • Atomic models: PDB-5myj
  • Surface level: 0.035
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-5myj
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_3581.png

Downloads & links

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Map

FileDownload / File: emd_3581.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.11 Å/pix.
x 600 pix.
= 663. Å		1.11 Å/pix.
x 600 pix.
= 663. Å		1.11 Å/pix.
x 600 pix.
= 663. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.105 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.035 / Movie #1: 0.035
Minimum - Maximum-0.0636561 - 0.16130547
Average (Standard dev.)0.00072141824 (±0.006043833)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions600600600
Spacing600600600
CellA=B=C: 663.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.1051.1051.105
M x/y/z600600600
origin x/y/z0.0000.0000.000
length x/y/z663.000663.000663.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS600600600
D min/max/mean-0.0640.1610.001

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Supplemental data

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Supplemental map: emd 3581 additional.map

Fileemd_3581_additional.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 70S ribosome

EntireName: 70S ribosome
Components
  • Complex: 70S ribosome
    • RNA: 16S ribosomal RNA
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14 type Z
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • Protein or peptide: 30S ribosomal protein S21
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L31 type B
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33 3
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • RNA: 23S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: Ribosome hibernation promotion factor

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Supramolecule #1: 70S ribosome

SupramoleculeName: 70S ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#51
Source (natural)Organism: Lactococcus lactis (lactic acid bacteria)

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Macromolecule #1: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Lactococcus lactis subsp. cremoris MG1363 (lactic acid bacteria)
Molecular weightTheoretical: 497.142344 KDa
SequenceString: UUGAGAGUUU GAUCCUGGCU CAGGACGAAC GCUGGCGGCG UGCCUAAUAC AUGCAAGUUG AGCGAUGAAG AUUGGUGCUU GCACCAAUU UGAAGAGCAG CGAACGGGUG AGUAACGCGU GGGGAAUCUG CCUUUGAGCG GGGGACAACA UUUGGAAACG A AUGCUAAU ...String:
UUGAGAGUUU GAUCCUGGCU CAGGACGAAC GCUGGCGGCG UGCCUAAUAC AUGCAAGUUG AGCGAUGAAG AUUGGUGCUU GCACCAAUU UGAAGAGCAG CGAACGGGUG AGUAACGCGU GGGGAAUCUG CCUUUGAGCG GGGGACAACA UUUGGAAACG A AUGCUAAU ACCGCAUAAU AACUUUAAAC AUAAGUUUUA AGUUUGAAAG AUGCAAUUGC AUCACUCAAA GAUGAUCCCG CG UUGUAUU AGCUAGUUGG UGAGGUAAAG GCUCACCAAG GCGAUGAUAC AUAGCCGACC UGAGAGGGUG AUCGGCCACA UUG GGACUG AGACACGGCC CAAACUCCUA CGGGAGGCAG CAGUAGGGAA UCUUCGGCAA UGGACGAAAG UCUGACCGAG CAAC GCCGC GUGAGUGAAG AAGGUUUUCG GAUCGUAAAA CUCUGUUGGU AGAGAAGAAC GUUGGUGAGA GUGGAAAGCU CAUCA AGUG ACGGUAACUA CCCAGAAAGG GACGGCUAAC UACGUGCCAG CAGCCGCGGU AAUACGUAGG UCCCGAGCGU UGUCCG GAU UUAUUGGGCG UAAAGCGAGC GCAGGUGGUU UAUUAAGUCU GGUGUAAAAG GCAGUGGCUC AACCAUUGUA UGCAUUG GA AACUGGUAGA CUUGAGUGCA GGAGAGGAGA GUGGAAUUCC AUGUGUAGCG GUGAAAUGCG UAGAUAUAUG GAGGAACA C CGGUGGCGAA AGCGGCUCUC UGGCCUGUAA CUGACACUGA GGCUCGAAAG CGUGGGGAGC AAACAGGAUU AGAUACCCU GGUAGUCCAC GCCGUAAACG AUGAGUGCUA GAUGUAGGGA GCUAUAAGUU CUCUGUAUCG CAGCUAACGC AAUAAGCACU CCGCCUGGG GAGUACGACC GCAAGGUUGA AACUCAAAGG AAUUGACGGG GGCCCGCACA AGCGGUGGAG CAUGUGGUUU A AUUCGAAG CAACGCGAAG AACCUUACCA GGUCUUGACA UACUCGUGCU AUUCCUAGAG AUAGGAAGUU CCUUCGGGAC AC GGGAUAC AGGUGGUGCA UGGUUGUCGU CAGCUCGUGU CGUGAGAUGU UGGGUUAAGU CCCGCAACGA GCGCAACCCC UAU UGUUAG UUGCCAUCAU UAAGUUGGGC ACUCUAACGA GACUGCCGGU GAUAAACCGG AGGAAGGUGG GGAUGACGUC AAAU CAUCA UGCCCCUUAU GACCUGGGCU ACACACGUGC UACAAUGGAU GGUACAACGA GUCGCGAGAC AGUGAUGUUU AGCUA AUCU CUUAAAACCA UUCUCAGUUC GGAUUGUAGG CUGCAACUCG CCUACAUGAA GUCGGAAUCG CUAGUAAUCG CGGAUC AGC ACGCCGCGGU GAAUACGUUC CCGGGCCUUG UACACACCGC CCGUCACACC ACGGGAGUUG GGAGUACCCG AAGUAGG UU GCCUAACCGC AAGGAGGGCG CUUCCUAAGG UAAGACCGAU GACUGGGGUG AAGUCGUAAC AAGGUAGCCG UAUCGGAA G GUGCGGCUGG AUCA

GENBANK: GENBANK: AM406671.1

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Macromolecule #31: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 31 / Number of copies: 1
Source (natural)Organism: Lactococcus lactis subsp. cremoris MG1363 (lactic acid bacteria)
Molecular weightTheoretical: 938.591125 KDa
SequenceString: CAAAGUUAAU AAGGGCGCAC GGUGGAUGCC UUGGCACUAA GAGCCGAUGA AGGACGUGAC UAACGACGAU AUUCUAGGGG GAGCAGUAA GUACGCAUUG AUCCCUAGGU CUCCGAAUGG GGAAACCCAG CUGUUACUAG CAGUUAUUCA UGAGUGAAUA C AUAGCUCA ...String:
CAAAGUUAAU AAGGGCGCAC GGUGGAUGCC UUGGCACUAA GAGCCGAUGA AGGACGUGAC UAACGACGAU AUUCUAGGGG GAGCAGUAA GUACGCAUUG AUCCCUAGGU CUCCGAAUGG GGAAACCCAG CUGUUACUAG CAGUUAUUCA UGAGUGAAUA C AUAGCUCA UGUAAAGGUA ACGCAGAGAA CUGAAACAUC UAAGUACCUG CAGGAAGAGA AAGUAAAAAC GAUUUCGUAA GU AGCGGCG AGCGAACGCG AAGAAGGGCA AACCAAGAAG CUUGCUUCUU GGGGUUGUAG GACUGCAACG UGGACUUAAG CAU UAUAGU CGAAUAACCU GGGAAGGUUA AUCAAAGAGG GUAAUAAUCC CGUAGACAAA AUAGCGCUUA UACCUAGCAG UAUC CUGAG UAGGGCUGGA CACGCGAAAU CCAGUUUGAA UCCGGGAGGA CCAUCUCCCA ACCCUAAAUA CUCCUUAGUG ACCGA UAGU GAACCAGUAC CGUGAGGGAA AGGUGAAAAG AACCCCGAGA GGGGAGUGAA AUAGCACCUG AAACCGUGUG CCUACA AGA AGUUCGAGCC CGUUAAUGGG UGAGAGCGUG CCUUUUGUAG AAUGAACCGG CGAGUUACGU UAUGAUGCGA GGUUAAG UU GAAGAGACGG AGCCGUAGGG AAACCGAGUC UGAAUAGGGC GACUUAGUAU CAUGAUGUAG ACCCGAAACC UAGUGACC U AUCCAUGAGC AGGGUGAAGG UGUGGUAAGA CGCACUGGAG GCCCGAACCA GGACACGUUG AAAAGUGUUU GGAUGACUU GUGGAUAGCG GAGAAAUUCC AAACGAACUG GGAGAUAGCU GGUUCUCUCC GAAAUAGCUU UAGGGCUAGC GUCGAAAUGU AAGUGUAUU GGAGGUAGAG CACUGUUUGG GUGAGGGGUC CGUCUAGGAU UACCAAUCUC AGAUAAACUC CGAAUGCUAA U ACACAUGU UCGGCAGUCA GACUGCGAGU GCUAAGAUCC GUAGUCGAAA GGGAAACAGC CCAGACCAAC AGCUAAGGUC CC AAAAUAU AUGUUAAGUG GAAAAGGAUG UGGGGUUGCA CAGACAACUA GGAUGUUAGC UCAGAAGCAG CUAUCAUUCA AAG AGUGCG UAAUAGCUCA CUAGUCGAGU GACCCUGCGC CGAAAAUGUA CCGGGGCUAA ACAUAUUACC GAAGCUUUGG AUUG AUAUU UUAUCAAUGG UAGGAGAGCG UUCUUAACCG CAAUGAAGGU AUACCGUGAG GAGUGCUGGA GCGUUAAGAA GUGAG AAUG CCGGUAUGAG UAGCGCAAGA UAAGUGAGAA UCUUAUCCAC CGUAAGACUA AGGUUUCCAG GGGAAGGCUC GUCCGC CCU GGGUUAGUCG GGACCUAAGG CGAGGCCGAA AGGCGUAGUC GAUGGACAAC UGGUUGAUAU UCCAGUACUA GAUAUGA UC GUGAUGGAGG GACGCAGUAG GCUAAGAGAU GCCAGUUAAU GGAUCCUGGU CUAAGCAGUG AGGUGUGAGA UGUGUCAA A UGCAUUUCUC UCUAACAUUG AGCUGUGAUG GGGAAGCAAC UACGGUUGCG AACUCUCUGA UGUCACACUG CCAAGAAAA GCUUCUAGCG UAAAGUCAUA UCUACCCGUA CCGCAAACCG ACACAGGUAG UCGAGGCGAG UAGCCUCAGG UGAUCGAGAG AACUCUCGU UAAGGAACUC GGCAAAAUAG CCCCGUAACU UCGGGAGAAG GGGUGCUGGU GUAAAAGCCA GCCGCAGUGA A UAGGCCCA AGCAACUGUU UAUCAAAAAC ACAGCUCUCU GCUAAACCGC AAGGUGAUGU AUAGGGGGUG ACGCCUGCCC GG UGCUGGA AGGUUAAGAG GAGUGCUUAG ACGUAAGUCG AAGGUAUGAA UUGAAGCCCC AGUAAACGGC GGCCGUAACU AUA ACGGUC CUAAGGUAGC GAAAUUCCUU GUCGGGUAAG UUCCGACCCG CACGAAAGGC GUAAUGAUUU GGGCACUGUC UCAA CGAGA GACUCGGUGA AAUUUUAGUA CCUGUGAAGA UGCAGGUUAC CCGCGACAGG ACGGAAAGAC CCCAUGGAGC UUUAC UGUA GUUUGAUAUU GAGUACCUGU AAGUCAUGUA CAGGAUAGGU AGGAGCCAUU GAAAUAGGGA CGCUAGUUUC UAUUGA GGC GUUGUUGGGA UACUACCCUU GACUUAUGGU UACUCUAACC CGCUGGCAUA AUCGGCCAGG GAGACAGUGU CUGACGG AC AGUUUGACUG GGGCGGUCGC CUCCUAAAGA GUAACGGAGG CGCUCAAAGG UUGGCUCAGA UUGGUUGGAA AUCAAUCG U AGAGUGUAAA GGUAAAAGCC AGCUUGACUG CGAGAGCUAC AACUCGAGCA GGUAGGAAAC UAGGACUUAG UGAUCCGGU GGUACCGCAU GGAAGGGCCA UCGCUCAACG GAUAAAAGCU ACCCUGGGGA UAACAGGCUU AUCUCCCCCA AGAGUUCACA UCGACGGGG AGGUUUGGCA CCUCGAUGUC GGCUCGUCGC AUCCUGGGGC UGUAGUCGGU CCCAAGGGUU GGGCUGUUCG C CCAUUAAA GCGGCACGCG AGCUGGGUUC AGAACGUCGU GAGACAGUUC GGUCCCUAUC CGUCGCGGGC GUAGGUAAUU UG AGAGGAU CUGUCCUUAG UACGAGAGGA CCGGGAUGGA CUUACCGCUG GUGUACCAGU UGUUCCGCCA GGAGCACGGC UGG AUAGCU AUGUAGGGAA GGGAUAAGCG CUGAAAGCAU CUAAGUGCGA AGCCCACCUC AAGAUGAGAU UACCCAUUCG UAAG AAUUA AGAGCCCAGA GAGAUGAUCU GGUAGAUAGG CUGGAAGUGG AAGAGUUGCG AGACUUGGAG CGGACCAGUA CUAAU CGCU CGAGGACUUU ACC

GENBANK: GENBANK: AM406671.1

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Macromolecule #32: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 32 / Number of copies: 1
Source (natural)Organism: Lactococcus lactis subsp. cremoris MG1363 (lactic acid bacteria)
Molecular weightTheoretical: 37.065969 KDa
SequenceString:
UUGGUAUUAA UUGCAUAGGA GAUAUACCUG UUCCCAUGUC GAACACAGAA GUCAAGUCCU AUUGCGCUGG AAGUACUUGG GGGUUGCCC CCUGGGAGAU AAAGACGAUG CCAAGU

GENBANK: GENBANK: AM406671.1

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Macromolecule #2: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 28.58976 KDa
SequenceString: MSVISMKQLL EAGVHFGHQT RRWNPKMKPY IFTERNGIHV IDLQKTVKLV DDAYNYVKNA SQEGAVVLFV GTKKQAAEAV KEEALRAGQ YYVNHRWLGG MLTNWNTIQT RVTRLKEINK MEEEGTFEVL PKKEVVLLNK ERERLEKFIG GIADMPRIPD V MYIVDPHA ...String:
MSVISMKQLL EAGVHFGHQT RRWNPKMKPY IFTERNGIHV IDLQKTVKLV DDAYNYVKNA SQEGAVVLFV GTKKQAAEAV KEEALRAGQ YYVNHRWLGG MLTNWNTIQT RVTRLKEINK MEEEGTFEVL PKKEVVLLNK ERERLEKFIG GIADMPRIPD V MYIVDPHA EQIAVKEAKT LGIPVVAMVD TNADPEPIDV VIPANDDAIR AVKLITAKMA DAIIEGRQGE DAAEDFVAEE AA SEESLEE LAEIVEGK

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #3: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 24.058795 KDa
SequenceString: MGQKVHPIGM RVGVIRDWDA KWYAEKEYAD YLHEDLAIRQ LIQTKLADAS VSLIETERAI NKVIVTLHTA KPGMVIGKSG ANVDALRAE LNKLTGKQVH INIVEIKKPD LDAHLVGEGI AKQLEARIAF RRAQKQAIQR AMRAGAKGIK TQVSGRLNGA D IARAEGYS ...String:
MGQKVHPIGM RVGVIRDWDA KWYAEKEYAD YLHEDLAIRQ LIQTKLADAS VSLIETERAI NKVIVTLHTA KPGMVIGKSG ANVDALRAE LNKLTGKQVH INIVEIKKPD LDAHLVGEGI AKQLEARIAF RRAQKQAIQR AMRAGAKGIK TQVSGRLNGA D IARAEGYS EGTVPLHTLR ADIDYAWEEA DTTYGKLGVK VWIYRGEVLP TKKSVKGEK

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #4: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 23.232605 KDa
SequenceString: MSRYTGPSWK QSRRYGISLT GSGKEIARRN YVPGQHGPNN RSKLSEYGLQ LAEKQKLRFT YGLSERQFRN LYVAATKVKE GTVGYNFMT LLEQRLDNVV YRLGLATTRR QARQFVNHGH ILVDGKRVDI PSFRVQPGQV ISVREKSMKV PAILEAVEAT K GRANFVSF ...String:
MSRYTGPSWK QSRRYGISLT GSGKEIARRN YVPGQHGPNN RSKLSEYGLQ LAEKQKLRFT YGLSERQFRN LYVAATKVKE GTVGYNFMT LLEQRLDNVV YRLGLATTRR QARQFVNHGH ILVDGKRVDI PSFRVQPGQV ISVREKSMKV PAILEAVEAT K GRANFVSF DADKLEGTLV RLPERDEINP EINDALIVEF YNKMM

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #5: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 17.609285 KDa
SequenceString:
MAENRRNDRE QSEFEERVVS INRVTKVVKG GRRLRFAALV VVGDRNGRVG FGTGKAQEVP EAIRKAIEAA KKNLITVPMV GTTLPHEAL GVFGGGKILL KPAVEGAGVA AGGAVRAVLE LAGVADVTSK SLGSNTPINV VRATVDGLTQ LKRAEEVAAL R GKSVSDFA

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #6: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 11.321831 KDa
SequenceString:
MTKYEILYII RPNIDEEAKT ALVERFDAIL TENGAANLES KDWEKRKLAY EINDFREGIY HIATFEAETT SEALSEFDRL AKINLDILR HMIVKVEA

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #7: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 17.713443 KDa
SequenceString:
MRKNRAPKRE VLADPMYNSI VVTRLINRVM LDGKRGVAAQ IVYGAFKQIE EATGNNPLEV FETAMENIMP VLEVRARRVG GSNYQVPVE VRPERRTTLG LRWLVTIARN RGEHTMQDRL AKEILDAANN TGAAVKKRED THKMAEANRA FAHFRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #8: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 14.692106 KDa
SequenceString:
MVMTDPIADF LTRIRNGNMR KFDVVEAPAS KIKRQIAEIL KAEGYVKDVE YVEDNKQGVI RVFLKYGKNG EKVITNLKRI SKPGLRVYV KSDDVPKVLN GLGTAIISTS TGVVTDKVAR QTNVGGEVIA YIW

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #9: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 14.124289 KDa
SequenceString:
MAQVQYAGTG RRKNAVARVR LVPGTGKITV NGREVESYIP HADMRLVINQ PFAATQTEGS YDTLVNVNGG GVSGQAGAIR HGIARALLQ VDPDFRSALK RAGLLTRDAR MVERKKPGLK KARKASQFSK R

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #10: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 11.764765 KDa
SequenceString:
MATKKIRIRL KAYEHRILDA AAEKIVETAK RTNAEVSGPI PLPTDRSVYT VIRATHKYKD SREQFEMRTH KRLIDIIEPT QKTVDSLMK LDLPSGVNIE IKL

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #11: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 13.310352 KDa
SequenceString:
MAKITRKRRV KKNIESGIVH IQSTFNNTIV MITDVHGNAL AWSSAGALGF KGSKKSTPFA AQMASEAAAK AAQEQGLKTV SVTVKGPGS GRESAIRALA AAGLNVTSIS DVTPVPHNGA RPPKRRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #12: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 15.157884 KDa
SequenceString:
MPTINQLVRK PRRAQVTKSK SPAMNVGYNS RKKVQTKLAS PQKRGVATRV GTMTPKKPNS ALRKFARVRL SNLMEVTAYI PGIGHNLQE HSVVLLRGGR VKDLPGVRYH IVRGALDTAG VADRKQSRSK YGAKKPKA

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #13: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 13.519672 KDa
SequenceString:
MARFAGVDIP NEKRIVISLT YVFGVGLQTS KKVLAAAGVS EDIRTKDLTS DQEDAIRREL DGLKLEGDLR REVSLNIKRL MEIGSYRGM RHRRGLPTRG QNTKNNARTR KGPAKSIAGK KK

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #14: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 7.151611 KDa
SequenceString:
MAKKSMVVKN QRPAKFSTQA YTRCERCGRP HSVYRKFKLC RICLRELAYK GQLPGVKKAS W

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #15: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 10.367041 KDa
SequenceString:
MAISKEKKQE IIAQYARKEG DTGSPEVQIA VLTWEINHLN DHIKSHKKDH ATQRGLMKKI GHRRNLLGYL RGKDVQRYRE LIASLGLRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #16: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 10.270938 KDa
SequenceString:
MSVKIRLTRM GSKKKPFYRI NVADSRAPRD GKFIETVGTY NPLVTENQVT LKEERVLEWL SNGAQPSDTV RNLLSKAGVM KKFHESKLS K

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #17: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 10.166831 KDa
SequenceString:
MERKQRKVYQ GRVVSDKMDK TITVVVETKR NHPVYGKRIN YSKKYKAHDE NNSAKTGDIV RIMETRPLSK DKHFRLVEIV EEAVII

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #18: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 9.392087 KDa
SequenceString:
MAQQRRGGFK RRKKVDFIAA NKIEVVDYKD TELLKRFISE RGKILPRRVT GTSAKNQRKV VNAIKRARVM ALLPFVAEDQ N

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #19: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 10.595238 KDa
SequenceString:
MGRSLKKGPF VDEHLMKKVE AQTNAERKSV IKTWSRRSTI FPNFVGLTIA VYDGRKHVPV YVQEDMVGHK LGEFAPTRTY RGHAADDKK TRR

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #20: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 8.38474 KDa
SequenceString:
MANIKSAIKR AELNKIANER NAQQKSAMRT LIKKFEAAPS EELYRAASST IDKAASKGLI HANKASRDKA RLAAKLG

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #21: 30S ribosomal protein S21

MacromoleculeName: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 7.041308 KDa
SequenceString:
MSKTLVRKNE SLDDALRRFK RSVTKAGTLQ ELRKREHYEK PSVKRKRKSE AARKRKKY

UniProtKB: Small ribosomal subunit protein bS21

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Macromolecule #22: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 7.187417 KDa
SequenceString:
MSKECYFTGR KTVSSNNRSH AMNQTKRVVK PNLQKVTILE NGELKTVWAS AKALKKLPAG VERV

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #23: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 7.874178 KDa
SequenceString:
MKLSETKSLL KDLRALSVEE LTTREAELKK ELFDLRFQAA AGRLENTAKL DEVKKTIARV KTVQAELNK

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #24: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 6.202401 KDa
SequenceString:
MAQIKITLVN SPIGRIPAQR KTVKALGLGK LNSSVVKEGS PAILGMVNSI SHLVKVEEA

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #25: 50S ribosomal protein L31 type B

MacromoleculeName: 50S ribosomal protein L31 type B / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 9.366479 KDa
SequenceString:
MKQNIHPNYQ PVVFMDTTTG YKFLTGSTKG SKETVEWEDG NTYPLIRVEI SSDSHPFYTG RQKFQAADGR IARFEKKYGK Q

UniProtKB: Large ribosomal subunit protein bL31B

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Macromolecule #26: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 6.681631 KDa
SequenceString:
MAVPARHTSS AKKNRRRTHY KLTAPTVTFD ETTGDYRHSH RVSLKGYYKG RKVRDTK

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #27: 50S ribosomal protein L33 3

MacromoleculeName: 50S ribosomal protein L33 3 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 5.567652 KDa
SequenceString:
MLRKAGLACT VCGSRNYTLN LSSVAKEKRV EVKKFCRTCG KHTLHKETR

UniProtKB: Large ribosomal subunit protein bL33C

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Macromolecule #28: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 5.200206 KDa
SequenceString:
MKRTYQPHKK SRKTTHGFRS RMATKNGRRV LAARRRKGRA SLTV

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #29: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 7.844419 KDa
SequenceString:
MPKQKTHRAS AKRFKRTGNG GLKRFRAYTS HRFHGKSVKQ RRQLRKASMV SKGDFKRIRR MVATMK

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #30: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 4.432446 KDa
SequenceString:
MKVRPSVKPI CEYCKVIRRN GRVMVICPAN PKHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #33: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 29.641348 KDa
SequenceString: MGIKVYKPTT NGRRNMTGSD FAEITTSTPE KSLLVSMSKT AGRNNTGRIT VRHHGGGHKR KYRMIDFKRT TDNVVAKVAT IEYDPNRTA NIALIVYANG VKSYILAAKG LEVGMTVVSG PDADIKVGNA LPLANIPVGT LIHNIELKPG KGGQLVRSAG A SAQVLGSE ...String:
MGIKVYKPTT NGRRNMTGSD FAEITTSTPE KSLLVSMSKT AGRNNTGRIT VRHHGGGHKR KYRMIDFKRT TDNVVAKVAT IEYDPNRTA NIALIVYANG VKSYILAAKG LEVGMTVVSG PDADIKVGNA LPLANIPVGT LIHNIELKPG KGGQLVRSAG A SAQVLGSE GKYTLVRLQS GEVRMILSTC RATIGVVGNE QQSLINLGKA GRTRHMGIRP TVRGSVMNPN DHPHGGGEGR QP VGRKSPM TPWGKPALGL KTRNKKAKSS KLIVRRIND

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #34: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 21.956568 KDa
SequenceString: MSKGILGKKV GMTQIFTDNG ELIPVTVIEA TPNTVLQVKS VETDGYEATQ VGFDTLREVL TNKPAKGHAA KANTTPKRFV REFKGLEGA EVGAEITVDT FAAGDVVDVT GTSKGKGFQG PIKRHGQSRG PMAHGSRYHR RPGSMGPVAA NKVPKGKKLA G RMGNKRVT ...String:
MSKGILGKKV GMTQIFTDNG ELIPVTVIEA TPNTVLQVKS VETDGYEATQ VGFDTLREVL TNKPAKGHAA KANTTPKRFV REFKGLEGA EVGAEITVDT FAAGDVVDVT GTSKGKGFQG PIKRHGQSRG PMAHGSRYHR RPGSMGPVAA NKVPKGKKLA G RMGNKRVT VQNLVIAQVL PEKNVILVKG NVPGAKKSLI VVKSAIKAK

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #35: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 22.393461 KDa
SequenceString: MAKVSLFKQD GSQAGEVTLN DSVFGIEPNE SVVFDVVISQ RASLRQGTHA HKNRSAVSGG GKKPWRQKGT GRARQGSTRS PQWRGGGTV FGPNPRSYAY KLPQKVRQLA LKSVYSTKVT DGKLIAVDTL DFAAPKTAEF AKVISALSIE RKVLVVLPNE G NEFAELSA ...String:
MAKVSLFKQD GSQAGEVTLN DSVFGIEPNE SVVFDVVISQ RASLRQGTHA HKNRSAVSGG GKKPWRQKGT GRARQGSTRS PQWRGGGTV FGPNPRSYAY KLPQKVRQLA LKSVYSTKVT DGKLIAVDTL DFAAPKTAEF AKVISALSIE RKVLVVLPNE G NEFAELSA RNLENVKVTT ANSASVLDIV SADKLLVVQS ALTQIEEVLA

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #36: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 20.03427 KDa
SequenceString:
MTNRLKEKYT NEVVPALTEQ FNYTSIMAVP KVDKIVINMG VGDAVNNSKN LDKAVAELAL ISGQKPLITK AKKSVAAFRL REGMPIGAK VTLRGERMFE FLDKLVTVSL PRVRDFHGVS NKAFDGRGNY TLGVKEQLIF PEINYDDVDK VRGMDIVIVT T ANTDEESR ELLAKLGMPF AK

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #37: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 19.313266 KDa
SequenceString:
MSRIGNKVIV IPAGVSVEVN GATVTVKGPK GELVRSFNEN ITLEIAENEI TVKRPNDTKE MKMLHGTTRA LLANMVEGVS NGFSKALEM IGVGYRAQLQ GTKLVLSVGK SHQDEVEAPE NIKFVVATPT SIVVEGISKE AVGQTAAYIR SRRSPEPYKG K GIRYVGEY VRRKEGKTGK

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #38: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 16.192855 KDa
SequenceString:
MMKTTFMANA QNVDRKWYVV DATDVPLGRL SAVVASVLRG KNKPTYTPHA DTGDFVIVVN AEKIKLTGKK ATDKVYYSHS LHPGGLKSV TAGELREKNA VRLIEKSVKG MLPHNTLGRA QGMKLKVFVG AEHTHTAQKP EVLDITGLI

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #39: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 12.944098 KDa
SequenceString:
MIQTESRLKV ADNSGAKELL TIRVLGGSSR KFAGIGDIVV ATVKSAAPGG AVKKGEVVKA VIVRTKSGAK RPDGSYIKFD ENAAVLIRD DKTPRGTRIF GPVARELREG GYMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #40: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 15.376553 KDa
SequenceString:
MELHSLKAAE GSRKVRNRVG RGTSSGNGKT SGRGQKGQKS RSGGGVRPGF EGGQTELFRR MPKRGFLNVN RKEYAIVNLE TLNRLEDGA TVSAETLVAA KIVKDVKSGV KVLANGELTA KNLTVKVAKV SAAAKAAIEA AGGSVEEA

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #41: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 15.375058 KDa
SequenceString:
MLVPKRVKHR REFRGKMRGY AKGGDTVSFG EYGLQATTSH WITNRQIEAA RIAMTRYMKR NGQVWIKIFP HKSYTAKAIG VRMGSGKGA PEGWVAPVKR GVVMFELGGV DEATAREALR LASHKLPVKT KFVKRGEA

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #42: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 14.280492 KDa
SequenceString:
MSNRKLGRTS SQRKAMLRDL TTDLIINETI VTTEARAKEV RRTVEKMITL GKKGDLSARR AAAAYVRNEI AIKDFNEETE TFPTALQKL FNDLAKRYEG RNGGYTRILK VEPRRGDAAP MAIIELV

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #43: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 12.426203 KDa
SequenceString:
MISKPDKNKL RQKRHTRVRG KISGTTETPR LNVFRSNTNI YAQVIDDVTG TTLASASSLK LTGTKTEQAA EVGKLVAEAA KAKGVEEVV FDRGGYLYHG RVAALATAAR EAGLKF

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #44: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 13.129329 KDa
SequenceString:
MNLIESINAA QLRTDIPDFR PGDTVRVHAK VVEGTRERIQ IFEGVVIARK NSGINETYTV RKISNGVGVE RIFPVHTPRV EKIEVIRHG KVRRAKLYYL RALTGKKARI AERRR

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #45: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 13.726362 KDa
SequenceString:
MARVKGSVAT RKRRKRILKL AKGYYGAKHR LFKTAKEQVM NSYYYAFRDR RQKKRDFRKL WIARINAAAR MNGLSYSKLM HGLKLADIE VNRKMLADIA IADAAAFTAL AEEAKKALAK

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #46: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 11.444199 KDa
SequenceString:
MSNYAIIKTG GKQVKVEEGS VIYVEKLNVE AGQTVTFDEV IFVGGETTKV GAPLVEGATV VGEVEKHGKQ KKVVTFQYKP KKHSHRKQG HRQPYTKVVI KSVNA

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #47: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 12.49054 KDa
SequenceString:
MAEITSAKAT AKTVRVSPRK TRLVIDLIRG KRVADAIAIL KFTPTKAAVE VENVLNSAIA NAENNFGLEK ANLVVSETFI NEGPTMKRF RPRAKGSASP INKRTAHITV VVAEKE

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #48: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 10.819567 KDa
SequenceString:
MSLYDIIRKP IITEASMAAM DQKKYTFEVD SRAHKLLIKQ AVEAVFDVKV ASVNTISVKP KSKRVGKYTG TKSGYKKAIV TLTEDSKTI EIFGEDAE

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #49: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 10.897782 KDa
SequenceString:
MFVKTGDTVK VIAGKDRGTT GKVIKALPKV NKVVVEGVAI MKKHQKPNSE NPSGAILEIE APIHVSNVQV LDKNGVAGRV GYKVVDDKK VRFNKKSGEI LD

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #50: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 10.053518 KDa
SequenceString:
MLELNLQLFA HKKGGGSTSN GRDSQAKRLG AKASDGELVS GGSILFRQRG THIHPGTNVG RGGDDTLFAK IEGTVKFEMK RGKKHVSVY PVVAK

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #51: Ribosome hibernation promotion factor

MacromoleculeName: Ribosome hibernation promotion factor / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363
Molecular weightTheoretical: 21.345988 KDa
SequenceString:
MIKFNIRGEN VEVTDAIRAY VEDKIGKLDK YFNDGHEVTA YVNLKVYTEK RAKVEVTLPA KNVTLRAEDT SQDMYSSIDF VEEKLERQI RKYKTRMNRK PRNAVPTGQV FGDEFAPLDT TDEVAEDHVD IVRTKHVALK PMDAEEAVLQ MDMLGHDFYV F TDADSNGT HVVYRRTDGR YGLIETE

UniProtKB: Ribosome hibernation promotion factor

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 80 % / Chamber temperature: 291.15 K / Instrument: FEI VITROBOT MARK III

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Specialist opticsSpherical aberration corrector: Cs-corrector (CEOS, Heidelberg, Germany)
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 25.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: OTHER / Details: RANSAC in Xmipp
Final reconstructionNumber classes used: 2 / Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 5.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 43530
Initial angle assignmentType: RANDOM ASSIGNMENT / Software - Name: Xmipp
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION
Final 3D classificationSoftware - Name: RELION
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-5myj:
Structure of 70S ribosome from Lactococcus lactis

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