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- PDB-5myj: Structure of 70S ribosome from Lactococcus lactis -

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Entry
Database: PDB / ID: 5myj
TitleStructure of 70S ribosome from Lactococcus lactis
Components
  • (30S ribosomal protein ...) x 20
  • (50S ribosomal protein ...) x 27
  • 16S ribosomal RNA
  • 23S ribosomal RNA
  • 5S ribosomal RNA
  • Ribosome hibernation promotion factor
KeywordsRIBOSOME / ribosome / 70S / lactoccocus lactis / Cryo-EM
Function / homologyRibosomal protein L17 superfamily / Ribosomal protein S7p/S5e / Ribosomal protein S18 superfamily / Ribosomal protein L13 superfamily / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Ribosomal protein L10e/L16 superfamily / Ribosomal protein S11 superfamily / RNA-binding S4 domain superfamily / Ribosomal protein L28/L24 superfamily / Ribosomal protein L35 superfamily ...Ribosomal protein L17 superfamily / Ribosomal protein S7p/S5e / Ribosomal protein S18 superfamily / Ribosomal protein L13 superfamily / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Ribosomal protein L10e/L16 superfamily / Ribosomal protein S11 superfamily / RNA-binding S4 domain superfamily / Ribosomal protein L28/L24 superfamily / Ribosomal protein L35 superfamily / Ribosomal protein S21 superfamily / Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain superfamily / Ribosomal protein L33 superfamily / Ribosomal protein L19 superfamily / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S12/S23 / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein S10 domain superfamily / Ribosomal protein S3, C-terminal domain / Ribosomal protein S19 / Ribosomal protein L22p/L17e / Ribosomal protein L14p/L23e / Ribosomal protein L16p/L10e / Ribosomal protein S14p/S29e / Ribosomal protein L23 / Ribosomal protein L5 / Ribosomal protein L3 / Ribosomal protein S15 / Ribosomal protein S2 / Ribosomal protein L30p/L7e / Ribosomal protein S5, N-terminal domain / Ribosomal protein S10p/S20e / Ribosomal protein L14 superfamily / Ribosomal protein S7 domain superfamily / Ribosomal protein S17 / Ribosomal protein L5, C-terminal / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S14, type Z / Ribosomal protein L13, conserved site / Ribosomal protein L4 domain superfamily / Ribosomal protein S19, superfamily / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S7 domain / Ribosomal protein S16 domain superfamily / Ribosomal protein L28/L24 / 30s ribosomal protein S13, C-terminal / Ribosomal protein S10 domain / Ribosomal protein L31 type B / Ribosomal protein L21-like / Ribosomal protein L15 / Ribosomal protein L5, N-terminal / Ribosomal protein L6, alpha-beta domain superfamily / L21-like superfamily / Ribosome hibernation promotion factor-like / Ribosomal protein S20 superfamily / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein L22/L17 superfamily / Ribosomal protein L24 signature. / Ribosomal L18e/L15P superfamily / Ribosomal protein L29/L35 superfamily / Sigma 54 modulation/S30EA ribosomal protein, C-terminal / Ribosomal protein S8 superfamily / Ribosomal protein S6 superfamily / Ribosomal protein L36 superfamily / Ribosomal protein L20, C-terminal / L28p-like / Ribosome hibernation promoting factor, long/plastid / Ribosomal protein L6 / Ribosomal protein S9/S16 / Ribosomal protein S4/S9 / Ribosomal protein L15 signature. / Ribosomal protein S7 signature. / Ribosomal protein S8 signature. / Ribosomal protein S11 signature. / Ribosomal protein S12 signature. / Ribosomal protein S17 signature. / Ribosomal protein S18 signature. / Ribosomal protein S19 signature. / Ribosomal protein L5 signature. / Ribosomal protein S9 signature. / Ribosomal protein S10 signature. / Ribosomal protein S15 signature. / Ribosomal protein L22 signature. / Ribosomal protein L2 signature. / Ribosomal protein L3 signature. / Ribosomal protein L6 signature 1. / Ribosomal protein L14 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L20 signature. / Ribosomal protein L35 signature. / Ribosomal protein L27 signature. / Ribosomal protein L36 signature. / Ribosomal protein L34 signature. / Ribosomal protein L13 signature. / Ribosomal protein S13 signature. / Ribosomal protein S14 signature. / Ribosomal protein L30 signature. / Ribosomal protein S4 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein S5 signature.
Function and homology information
Specimen sourceLactococcus lactis subsp. cremoris MG1363 (lactic acid bacteria)
Lactococcus lactis subsp. cremoris (lactic acid bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / 5.6 Å resolution
AuthorsFranken, L.E. / Oostergetel, G.T. / Pijning, T. / Puri, P. / Boekema, E.J. / Poolman, B. / Guskov, A.
CitationJournal: Nat Commun / Year: 2017
Title: A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy.
Authors: Linda E Franken / Gert T Oostergetel / Tjaard Pijning / Pranav Puri / Valentina Arkhipova / Egbert J Boekema / Bert Poolman / Albert Guskov
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Jan 26, 2017 / Release: Oct 11, 2017

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Assembly

Deposited unit
AA: 16S ribosomal RNA
AB: 30S ribosomal protein S2
AC: 30S ribosomal protein S3
AD: 30S ribosomal protein S4
AE: 30S ribosomal protein S5
AF: 30S ribosomal protein S6
AG: 30S ribosomal protein S7
AH: 30S ribosomal protein S8
AI: 30S ribosomal protein S9
AJ: 30S ribosomal protein S10
AK: 30S ribosomal protein S11
AL: 30S ribosomal protein S12
AM: 30S ribosomal protein S13
AN: 30S ribosomal protein S14 type Z
AO: 30S ribosomal protein S15
AP: 30S ribosomal protein S16
AQ: 30S ribosomal protein S17
AR: 30S ribosomal protein S18
AS: 30S ribosomal protein S19
AT: 30S ribosomal protein S20
AU: 30S ribosomal protein S21
B0: 50S ribosomal protein L28
B1: 50S ribosomal protein L29
B2: 50S ribosomal protein L30
B3: 50S ribosomal protein L31 type B
B4: 50S ribosomal protein L32
B5: 50S ribosomal protein L33 3
B6: 50S ribosomal protein L34
B7: 50S ribosomal protein L35
B8: 50S ribosomal protein L36
BA: 23S ribosomal RNA
BB: 5S ribosomal RNA
BD: 50S ribosomal protein L2
BE: 50S ribosomal protein L3
BF: 50S ribosomal protein L4
BG: 50S ribosomal protein L5
BH: 50S ribosomal protein L6
BM: 50S ribosomal protein L13
BN: 50S ribosomal protein L14
BO: 50S ribosomal protein L15
BP: 50S ribosomal protein L16
BQ: 50S ribosomal protein L17
BR: 50S ribosomal protein L18
BS: 50S ribosomal protein L19
BT: 50S ribosomal protein L20
BU: 50S ribosomal protein L21
BV: 50S ribosomal protein L22
BW: 50S ribosomal protein L23
BX: 50S ribosomal protein L24
BZ: 50S ribosomal protein L27
A: Ribosome hibernation promotion factor


Theoretical massNumber of molelcules
Total (without water)2,115,46251
Polyers2,115,46251
Non-polymers00
Water0
1


  • idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area (Å2)240680
ΔGint (kcal/M)-1878
Surface area (Å2)742030
MethodPISA

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Components

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RNA chain , 3 types, 3 molecules AABABB

#1: RNA chain 16S ribosomal RNA /


Mass: 497142.344 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris MG1363 (lactic acid bacteria)
References: GenBank: 124491690
#31: RNA chain 23S ribosomal RNA /


Mass: 938591.125 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris MG1363 (lactic acid bacteria)
References: GenBank: 124491690
#32: RNA chain 5S ribosomal RNA /


Mass: 37065.969 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris MG1363 (lactic acid bacteria)
References: GenBank: 124491690

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30S ribosomal protein ... , 20 types, 20 molecules ABACADAEAFAGAHAIAJAKALAMANAOAPAQARASATAU

#2: Protein/peptide 30S ribosomal protein S2 /


Mass: 28589.760 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNV0
#3: Protein/peptide 30S ribosomal protein S3 /


Mass: 24058.795 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNP9
#4: Protein/peptide 30S ribosomal protein S4 /


Mass: 23232.605 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RI10
#5: Protein/peptide 30S ribosomal protein S5 /


Mass: 17609.285 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNN6
#6: Protein/peptide 30S ribosomal protein S6 /


Mass: 11321.831 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNZ4
#7: Protein/peptide 30S ribosomal protein S7 /


Mass: 17713.443 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RP73
#8: Protein/peptide 30S ribosomal protein S8 /


Mass: 14692.106 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNN9
#9: Protein/peptide 30S ribosomal protein S9 /


Mass: 14124.289 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RP61
#10: Protein/peptide 30S ribosomal protein S10 /


Mass: 11764.765 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNQ6
#11: Protein/peptide 30S ribosomal protein S11 /


Mass: 13310.352 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNM8
#12: Protein/peptide 30S ribosomal protein S12 /


Mass: 15157.884 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RP74
#13: Protein/peptide 30S ribosomal protein S13 /


Mass: 13519.672 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNM9
#14: Protein/peptide 30S ribosomal protein S14 type Z / Ribosome


Mass: 7151.611 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNP2
#15: Protein/peptide 30S ribosomal protein S15 /


Mass: 10367.041 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RMV9
#16: Protein/peptide 30S ribosomal protein S16 /


Mass: 10270.938 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RJS1
#17: Protein/peptide 30S ribosomal protein S17 /


Mass: 10166.831 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNP6
#18: Protein/peptide 30S ribosomal protein S18 /


Mass: 9392.087 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNZ2
#19: Protein/peptide 30S ribosomal protein S19 /


Mass: 10595.238 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNQ1
#20: Protein/peptide 30S ribosomal protein S20 /


Mass: 8384.740 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RMG0
#21: Protein/peptide 30S ribosomal protein S21 /


Mass: 7041.308 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RHW9

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50S ribosomal protein ... , 27 types, 27 molecules B0B1B2B3B4B5B6B7B8BDBEBFBGBHBMBNBOBPBQBRBSBTBUBVBWBXBZ

#22: Protein/peptide 50S ribosomal protein L28 /


Mass: 7187.417 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RHS3
#23: Protein/peptide 50S ribosomal protein L29 /


Mass: 7874.178 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNP7
#24: Protein/peptide 50S ribosomal protein L30 /


Mass: 6202.401 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNN5
#25: Protein/peptide 50S ribosomal protein L31 type B / Ribosome


Mass: 9366.479 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RJP7
#26: Protein/peptide 50S ribosomal protein L32 /


Mass: 6681.631 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RHH0
#27: Protein/peptide 50S ribosomal protein L33 3 / Ribosome


Mass: 5567.652 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNR2
#28: Protein/peptide 50S ribosomal protein L34 /


Mass: 5200.206 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RHL6
#29: Protein/peptide 50S ribosomal protein L35 /


Mass: 7844.419 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RMR2
#30: Protein/peptide 50S ribosomal protein L36 /


Mass: 4432.446 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNN0
#33: Protein/peptide 50S ribosomal protein L2 /


Mass: 29641.348 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNQ2
#34: Protein/peptide 50S ribosomal protein L3 /


Mass: 21956.568 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNQ5
#35: Protein/peptide 50S ribosomal protein L4 /


Mass: 22393.461 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNQ4
#36: Protein/peptide 50S ribosomal protein L5 /


Mass: 20034.270 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNP3
#37: Protein/peptide 50S ribosomal protein L6 /


Mass: 19313.266 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNN8
#38: Protein/peptide 50S ribosomal protein L13 /


Mass: 16192.855 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RP62
#39: Protein/peptide 50S ribosomal protein L14 /


Mass: 12944.098 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNP5
#40: Protein/peptide 50S ribosomal protein L15 /


Mass: 15376.553 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNN4
#41: Protein/peptide 50S ribosomal protein L16 /


Mass: 15375.058 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNP8
#42: Protein/peptide 50S ribosomal protein L17 /


Mass: 14280.492 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNM6
#43: Protein/peptide 50S ribosomal protein L18 /


Mass: 12426.203 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNN7
#44: Protein/peptide 50S ribosomal protein L19 /


Mass: 13129.329 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RLS5
#45: Protein/peptide 50S ribosomal protein L20 /


Mass: 13726.362 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RMR1
#46: Protein/peptide 50S ribosomal protein L21 /


Mass: 11444.199 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RLA4
#47: Protein/peptide 50S ribosomal protein L22 /


Mass: 12490.540 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNQ0
#48: Protein/peptide 50S ribosomal protein L23 /


Mass: 10819.567 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNQ3
#49: Protein/peptide 50S ribosomal protein L24 /


Mass: 10897.782 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RNP4
#50: Protein/peptide 50S ribosomal protein L27 /


Mass: 10053.518 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RLA2

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Protein/peptide , 1 types, 1 molecules A

#51: Protein/peptide Ribosome hibernation promotion factor / HPF / Lactococcus lactis hibernation promotion factor / LIHPF


Mass: 21345.988 Da / Num. of mol.: 1
Source: (natural) Lactococcus lactis subsp. cremoris (strain MG1363) (lactic acid bacteria)
Strain: MG1363 / References: UniProt: A2RIX0

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / Reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: 70S ribosomeRibosome / Type: RIBOSOME
Entity ID: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51
Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Lactococcus lactis (lactic acid bacteria)
Buffer solutionpH: 7.6
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 400 / Grid type: Quantifoil R2/2
VitrificationInstrument: FEI VITROBOT MARK III / Cryogen name: ETHANE / Humidity: 80 % / Chamber temperature: 291.15 kelvins

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 25 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k)
EM imaging opticsSph aberration corrector: Cs-corrector (CEOS, Heidelberg, Germany)

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Processing

EM software
IDNameVersionCategory
2EPUimage acquisition
4CTFFIND4CTF correction
7Cootmodel fitting
9PHENIX1.11.1model refinement
10Xmippinitial Euler assignment
11RELIONfinal Euler assignment
12RELIONclassification
13RELION1.43D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C1
3D reconstructionResolution: 5.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 43530 / Number of class averages: 2 / Symmetry type: POINT
Atomic model buildingRef protocol: FLEXIBLE FIT / Ref space: REAL
Least-squares processHighest resolution: 5.6 Å

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