[English] 日本語
Yorodumi
- EMDB-12535: Structure of the yeast Gcn1 bound to a colliding stalled 80S ribo... -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-12535
TitleStructure of the yeast Gcn1 bound to a colliding stalled 80S ribosome with MBF1, A/P-tRNA and P/E-tRNA
Map data
SampleStructure of the yeast Gcn1-bound coilliding stalled 80S ribosome with MBF1, Gir2, A/P-tRNA and P/E-tRNA
  • (nucleic-acidNucleic acid) x 7
  • (40S ribosomal protein ...) x 31
  • Ubiquitin-40S ribosomal protein S31
  • Guanine nucleotide-binding protein subunit beta-like protein
  • Multiprotein-bridging factor 1
  • (60S ribosomal protein ...) x 40
  • Ubiquitin-60S ribosomal protein L40
Function / homology
Function and homology information


maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / regulation of cellular response to stress / response to cycloheximide / invasive growth in response to glucose limitation / GDP-dissociation inhibitor activity / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / pre-mRNA 5'-splice site binding / rRNA export from nucleus ...maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / regulation of cellular response to stress / response to cycloheximide / invasive growth in response to glucose limitation / GDP-dissociation inhibitor activity / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / pre-mRNA 5'-splice site binding / rRNA export from nucleus / preribosome, large subunit precursor / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 90S preribosome / preribosome, small subunit precursor / ribonucleoprotein complex assembly / mRNA destabilization / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / rRNA methylation / rescue of stalled ribosome / negative regulation of mRNA splicing, via spliceosome / G-protein alpha-subunit binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / DNA-(apurinic or apyrimidinic site) endonuclease activity / ribosomal large subunit export from nucleus / ribosomal small subunit export from nucleus / maturation of SSU-rRNA / regulation of translational fidelity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / small-subunit processome / ribosomal small subunit biogenesis / protein kinase A catalytic subunit binding / maturation of LSU-rRNA / positive regulation of protein kinase activity / cellular response to amino acid starvation / ribosome assembly / assembly of large subunit precursor of preribosome / maintenance of translational fidelity / positive regulation of translational fidelity / translational termination / protein kinase C binding / ribosomal small subunit assembly / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / modification-dependent protein catabolic process / small ribosomal subunit rRNA binding / mRNA 5'-UTR binding / macroautophagy / protein tag / rRNA processing / large ribosomal subunit rRNA binding / cytoplasmic stress granule / cytoplasmic translation / cytosolic large ribosomal subunit / ribosomal large subunit assembly / ribosome binding / 5S rRNA binding / ribosome biogenesis / cytosolic small ribosomal subunit / small ribosomal subunit / protein localization / negative regulation of translation / ribosome / rRNA binding / transcription coactivator activity / structural constituent of ribosome / protein ubiquitination / translation / negative regulation of gene expression / positive regulation of protein phosphorylation / mRNA binding / G protein-coupled receptor signaling pathway / response to antibiotic / ubiquitin protein ligase binding / nucleolus / positive regulation of transcription by RNA polymerase II / mitochondrion / RNA binding / DNA binding / zinc ion binding / nucleoplasm / metal ion binding / nucleus / cytosol / cytoplasm
Ribosomal protein L30, conserved site / Ribosomal protein L19/L19e, domain 1 / Ribosomal S24e conserved site / Ribosomal protein S4, conserved site / Ribosomal protein L34e, conserved site / WD40-repeat-containing domain / Ribosomal protein L10e/L16 / Ribosomal protein L30, ferredoxin-like fold domain / K homology domain-like, alpha/beta / Ribosomal protein S2, conserved site ...Ribosomal protein L30, conserved site / Ribosomal protein L19/L19e, domain 1 / Ribosomal S24e conserved site / Ribosomal protein S4, conserved site / Ribosomal protein L34e, conserved site / WD40-repeat-containing domain / Ribosomal protein L10e/L16 / Ribosomal protein L30, ferredoxin-like fold domain / K homology domain-like, alpha/beta / Ribosomal protein S2, conserved site / WD40/YVTN repeat-like-containing domain superfamily / Ribosomal protein L2, domain 3 / Ribosomal protein L2, domain 2 / Ribosomal protein S5 domain 2-type fold, subgroup / Ribosomal protein S6, eukaryotic / Ribosomal protein S4e, central region / Ribosomal protein S4e, N-terminal / Ribosomal S11, conserved site / Ribosomal protein S5, N-terminal, conserved site / Multiprotein bridging factor 1, N-terminal / Ribosomal protein L37e, conserved site / Ribosomal protein S21e, conserved site / Ribosomal protein S19e, conserved site / Ribosomal protein S17e, conserved site / Ribosomal protein S14, conserved site / Ribosomal protein S13, conserved site / Ribosomal protein S10, conserved site / Ribosomal protein L35Ae, conserved site / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein L32e, conserved site / Ribosomal protein L27e, conserved site / Ribosomal protein L22/L17, conserved site / Ribosomal protein L21e, conserved site / Ribosomal protein L13e, conserved site / Ribosomal protein L10e, conserved site / Ribosomal protein L29, conserved site / Ribosomal protein S5, N-terminal / Ribosomal protein L25/L23 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S5/S7, eukaryotic/archaeal / KOW / Ribosomal protein L13 / Ribosomal protein L26/L24, eukaryotic/archaeal / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein S19A/S15e / Ribosomal protein L7, eukaryotic / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal protein L24/L26, conserved site / Ribosomal protein S12/S23 / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L37ae/L37e / Ribosomal protein L30, N-terminal / Ribosomal protein L23/L15e core domain superfamily / Nucleotide-binding alpha-beta plait domain superfamily / Ribosomal protein S13/S15, N-terminal / Nucleic acid-binding, OB-fold / Zinc-binding ribosomal protein / Lambda repressor-like, DNA-binding domain superfamily / Ribosomal protein S30 / Ribosomal protein S13-like, H2TH / S15/NS1, RNA-binding / K homology domain superfamily, prokaryotic type / Translation protein, beta-barrel domain superfamily / Translation protein SH3-like domain superfamily / Ribosomal protein L34Ae / Ribosomal protein L41 / Ribosomal protein S3, conserved site / Ribosomal protein S6e, conserved site / Ribosomal protein S5, C-terminal / Ribosomal protein S28e conserved site / Ribosomal protein L5, N-terminal / Ribosomal protein L5, C-terminal / Ribosomal protein L15 / Ubiquitin-like domain superfamily / 50S ribosomal protein L30e-like / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein S17, archaeal/eukaryotic / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S2, eukaryotic / Ribosomal protein S10 domain / 30s ribosomal protein S13, C-terminal / 60S ribosomal protein L4, C-terminal domain / Ribosomal protein L5 eukaryotic/L18 archaeal, C-terminal / Ribosomal protein L15e core domain superfamily / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein L19, eukaryotic / Ribosomal protein L19/L19e conserved site / Ribosomal protein L22/L17 superfamily / Ribosomal protein L14 superfamily / Ribosomal protein S10 domain superfamily / Ribosomal protein S7 domain superfamily
40S ribosomal protein S27-A / 40S ribosomal protein S0-A / 40S ribosomal protein S7-A / 60S ribosomal protein L16-A / 40S ribosomal protein S5 / 60S ribosomal protein L5 / 40S ribosomal protein S2 / 60S ribosomal protein L8-A / 40S ribosomal protein S17-B / 60S ribosomal protein L3 ...40S ribosomal protein S27-A / 40S ribosomal protein S0-A / 40S ribosomal protein S7-A / 60S ribosomal protein L16-A / 40S ribosomal protein S5 / 60S ribosomal protein L5 / 40S ribosomal protein S2 / 60S ribosomal protein L8-A / 40S ribosomal protein S17-B / 60S ribosomal protein L3 / 60S ribosomal protein L30 / 60S ribosomal protein L4-A / 60S ribosomal protein L41-B / 60S ribosomal protein L35-A / 60S ribosomal protein L19-A / 40S ribosomal protein S1-A / 40S ribosomal protein S10-A / 60S ribosomal protein L14-A / 60S ribosomal protein L38 / 40S ribosomal protein S25-A / 60S ribosomal protein L11-B / 60S ribosomal protein L13-A / 40S ribosomal protein S16-A / 60S ribosomal protein L21-A / 40S ribosomal protein S15 / 60S ribosomal protein L34-A / 60S ribosomal protein L37-A / Guanine nucleotide-binding protein subunit beta-like protein / 40S ribosomal protein S12 / 60S ribosomal protein L10 / 40S ribosomal protein S29-A / 40S ribosomal protein S26-B / 40S ribosomal protein S14-B / 40S ribosomal protein S20 / 60S ribosomal protein L32 / 40S ribosomal protein S18-A / 60S ribosomal protein L27-A / 60S ribosomal protein L18-A / 60S ribosomal protein L6-B / 60S ribosomal protein L15-A / 60S ribosomal protein L29 / 60S ribosomal protein L36-A / 60S ribosomal protein L33-A / 60S ribosomal protein L26-A / 60S ribosomal protein L17-A / 60S ribosomal protein L9-A / 40S ribosomal protein S3 / 60S ribosomal protein L7-A / 60S ribosomal protein L39 / 60S ribosomal protein L25 / 60S ribosomal protein L24-A / 60S ribosomal protein L28 / Multiprotein-bridging factor 1 / 40S ribosomal protein S9-A / 60S ribosomal protein L22-A / 40S ribosomal protein S13 / 40S ribosomal protein S11-A / 40S ribosomal protein S23-A / 60S ribosomal protein L2-A / 60S ribosomal protein L23-A / 40S ribosomal protein S8-A / 40S ribosomal protein S6-A / 40S ribosomal protein S4-A / 40S ribosomal protein S30-A / 40S ribosomal protein S24-A / 60S ribosomal protein L42-A / Ubiquitin-40S ribosomal protein S31 / 60S ribosomal protein L43-A / 60S ribosomal protein L20-A / Ubiquitin-60S ribosomal protein L40 / 60S ribosomal protein L31-A / 40S ribosomal protein S22-A / 40S ribosomal protein S21-A / 40S ribosomal protein S19-A / 40S ribosomal protein S28-A
Biological speciesSaccharomyces cerevisiae S288c (yeast) / Baker's yeast (baker's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.36 Å
AuthorsPochopien AA / Beckert B / Wilson DN
Funding support Germany, 2 items
OrganizationGrant numberCountry
German Research Foundation (DFG)WI3285/8-1 Germany
German Research Foundation (DFG)SPP-1879 Germany
CitationJournal: Proc Natl Acad Sci U S A / Year: 2021
Title: Structure of Gcn1 bound to stalled and colliding 80S ribosomes.
Authors: Agnieszka A Pochopien / Bertrand Beckert / Sergo Kasvandik / Otto Berninghausen / Roland Beckmann / Tanel Tenson / Daniel N Wilson /
Abstract: The Gcn pathway is conserved in all eukaryotes, including mammals such as humans, where it is a crucial part of the integrated stress response (ISR). Gcn1 serves as an essential effector protein for ...The Gcn pathway is conserved in all eukaryotes, including mammals such as humans, where it is a crucial part of the integrated stress response (ISR). Gcn1 serves as an essential effector protein for the kinase Gcn2, which in turn is activated by stalled ribosomes, leading to phosphorylation of eIF2 and a subsequent global repression of translation. The fine-tuning of this adaptive response is performed by the Rbg2/Gir2 complex, a negative regulator of Gcn2. Despite the wealth of available biochemical data, information on structures of Gcn proteins on the ribosome has remained elusive. Here we present a cryo-electron microscopy structure of the yeast Gcn1 protein in complex with stalled and colliding 80S ribosomes. Gcn1 interacts with both 80S ribosomes within the disome, such that the Gcn1 HEAT repeats span from the P-stalk region on the colliding ribosome to the P-stalk and the A-site region of the lead ribosome. The lead ribosome is stalled in a nonrotated state with peptidyl-tRNA in the A-site, uncharged tRNA in the P-site, eIF5A in the E-site, and Rbg2/Gir2 in the A-site factor binding region. By contrast, the colliding ribosome adopts a rotated state with peptidyl-tRNA in a hybrid A/P-site, uncharged-tRNA in the P/E-site, and Mbf1 bound adjacent to the mRNA entry channel on the 40S subunit. Collectively, our findings reveal the interaction mode of the Gcn2-activating protein Gcn1 with colliding ribosomes and provide insight into the regulation of Gcn2 activation. The binding of Gcn1 to a disome has important implications not only for the Gcn2-activated ISR, but also for the general ribosome-associated quality control pathways.
Validation ReportSummary, Full report, XML, About validation report
History
DepositionMar 3, 2021-
Header (metadata) releaseApr 14, 2021-
Map releaseApr 14, 2021-
UpdateApr 14, 2021-
Current statusApr 14, 2021Processing site: PDBe / Status: Released

-
Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.03
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.03
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-7nrd
  • Surface level: 0.03
  • Imaged by UCSF Chimera
  • Download
  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-7nrd
  • Imaged by Jmol
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

-
Map

FileDownload / File: emd_12535.map.gz / Format: CCP4 / Size: 1.3 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 700 pix.
= 758.8 Å
1.08 Å/pix.
x 700 pix.
= 758.8 Å
1.08 Å/pix.
x 700 pix.
= 758.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.084 Å
Density
Contour LevelBy AUTHOR: 0.03 / Movie #1: 0.03
Minimum - Maximum-0.31267092 - 0.5077412
Average (Standard dev.)0.0007687183 (±0.0064846477)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions700700700
Spacing700700700
CellA=B=C: 758.8 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0841.0841.084
M x/y/z700700700
origin x/y/z0.0000.0000.000
length x/y/z758.800758.800758.800
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS700700700
D min/max/mean-0.3130.5080.001

-
Supplemental data

+
Additional map: map colliding ribosome relion final run

Fileemd_12535_additional_1.map
Annotationmap colliding ribosome relion final run
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

+
Additional map: focus refinement 40S only and postprocess

Fileemd_12535_additional_2.map
Annotationfocus refinement 40S only and postprocess
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

+
Additional map: map colliding ribosome postprocess

Fileemd_12535_additional_3.map
Annotationmap colliding ribosome postprocess
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

+
Half map: Half map colliding ribosome

Fileemd_12535_half_map_1.map
AnnotationHalf map colliding ribosome
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

+
Half map: Half map colliding ribosome

Fileemd_12535_half_map_2.map
AnnotationHalf map colliding ribosome
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

-
Sample components

+
Entire Structure of the yeast Gcn1-bound coilliding stalled 80S ribosome...

EntireName: Structure of the yeast Gcn1-bound coilliding stalled 80S ribosome with MBF1, Gir2, A/P-tRNA and P/E-tRNA
Number of components: 83

+
Component #1: protein, Structure of the yeast Gcn1-bound coilliding stalled 80S...

ProteinName: Structure of the yeast Gcn1-bound coilliding stalled 80S ribosome with MBF1, Gir2, A/P-tRNA and P/E-tRNA
Recombinant expression: No
SourceSpecies: Saccharomyces cerevisiae S288c (yeast)

+
Component #2: nucleic-acid, TPA_inf: Saccharomyces cerevisiae S288C chromosome ...

nucleic acidName: TPA_inf: Saccharomyces cerevisiae S288C chromosome XII, complete sequence
Class: RNA / Structure: OTHER / Synthetic: No
Sequence: UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUCGUUUAU UUGAUAGUUC CUUUACUACA UGGUAUAACU GUGGUAAUUC UAGAGCUAAU ...Sequence:
UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUCGUUUAU UUGAUAGUUC CUUUACUACA UGGUAUAACU GUGGUAAUUC UAGAGCUAAU ACAUGCUUAA AAUCUCGACC CUUUGGAAGA GAUGUAUUUA UUAGAUAAAA AAUCAAUGUC UUCGGACUCU UUGAUGAUUC AUAAUAACUU UUCGAAUCGC AUGGCCUUGU GCUGGCGAUG GUUCAUUCAA AUUUCUGCCC UAUCAACUUU CGAUGGUAGG AUAGUGGCCU ACCAUGGUUU CAACGGGUAA CGGGGAAUAA GGGUUCGAUU CCGGAGAGGG AGCCUGAGAA ACGGCUACCA CAUCCAAGGA AGGCAGCAGG CGCGCAAAUU ACCCAAUCCU AAUUCAGGGA GGUAGUGACA AUAAAUAACG AUACAGGGCC CAUUCGGGUC UUGUAAUUGG AAUGAGUACA AUGUAAAUAC CUUAACGAGG AACAAUUGGA GGGCAAGUCU GGUGCCAGCA GCCGCGGUAA UUCCAGCUCC AAUAGCGUAU AUUAAAGUUG UUGCAGUUAA AAAGCUCGUA GUUGAACUUU GGGCCCGGUU GGCCGGUCCG AUUUUUUCGU GUACUGGAUU UCCAACGGGG CCUUUCCUUC UGGCUAACCU UGAGUCCUUG UGGCUCUUGG CGAACCAGGA CUUUUACUUU GAAAAAAUUA GAGUGUUCAA AGCAGGCGUA UUGCUCGAAU AUAUUAGCAU GGAAUAAUAG AAUAGGACGU UUGGUUCUAU UUUGUUGGUU UCUAGGACCA UCGUAAUGAU UAAUAGGGAC GGUCGGGGGC AUCAGUAUUC AAUUGUCAGA GGUGAAAUUC UUGGAUUUAU UGAAGACUAA CUACUGCGAA AGCAUUUGCC AAGGACGUUU UCAUUAAUCA AGAACGAAAG UUAGGGGAUC GAAGAUGAUC AGAUACCGUC GUAGUCUUAA CCAUAAACUA UGCCGACUAG GGAUCGGGUG GUGUUUUUUU AAUGACCCAC UCGGCACCUU ACGAGAAAUC AAAGUCUUUG GGUUCUGGGG GGAGUAUGGU CGCAAGGCUG AAACUUAAAG GAAUUGACGG AAGGGCACCA CCAGGAGUGG AGCCUGCGGC UUAAUUUGAC UCAACACGGG GAAACUCACC AGGUCCAGAC ACAAUAAGGA UUGACAGAUU GAGAGCUCUU UCUUGAUUUU GUGGGUGGUG GUGCAUGGCC GUUCUUAGUU GGUGGAGUGA UUUGUCUGCU UAAUUGCGAU AACGAACGAG ACCUUAACCU ACUAAAUAGU GGUGCUAGCA UUUGCUGGUU AUCCACUUCU UAGAGGGACU AUCGGUUUCA AGCCGAUGGA AGUUUGAGGC AAUAACAGGU CUGUGAUGCC CUUAGACGUU CUGGGCCGCA CGCGCGCUAC ACUGACGGAG CCAGCGAGUC UAACCUUGGC CGAGAGGUCU UGGUAAUCUU GUGAAACUCC GUCGUGCUGG GGAUAGAGCA UUGUAAUUAU UGCUCUUCAA CGAGGAAUUC CUAGUAAGCG CAAGUCAUCA GCUUGCGUUG AUUACGUCCC UGCCCUUUGU ACACACCGCC CGUCGCUAGU ACCGAUUGAA UGGCUUAGUG AGGCCUCAGG AUCUGCUUAG AGAAGGGGGC AACUCCAUCU CAGAGCGGAG AAUUUGGACA AACUUGGUCA UUUAGAGGAA CUAAAAGUCG UAACAAGGUU UCCGUAGGUG AACCUGCGGA AGGAUCAUUA
MassTheoretical: 579.761938 kDa
SourceSpecies: Saccharomyces cerevisiae S288C (yeast)

+
Component #3: protein, 40S ribosomal protein S3

ProteinName: 40S ribosomal protein S3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.702791 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #4: protein, 40S ribosomal protein S5

ProteinName: 40S ribosomal protein S5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.908338 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #5: protein, 40S ribosomal protein S10-A

ProteinName: 40S ribosomal protein S10-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.042562 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #6: protein, 40S ribosomal protein S12

ProteinName: 40S ribosomal protein S12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.327331 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #7: protein, 40S ribosomal protein S15

ProteinName: 40S ribosomal protein S15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.421732 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #8: protein, 40S ribosomal protein S16-A

ProteinName: 40S ribosomal protein S16-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.659216 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #9: protein, 40S ribosomal protein S17-B

ProteinName: 40S ribosomal protein S17-BRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.268545 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #10: protein, 40S ribosomal protein S18-A

ProteinName: 40S ribosomal protein S18-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.940443 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #11: protein, 40S ribosomal protein S19-A

ProteinName: 40S ribosomal protein S19-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.81093 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #12: protein, 40S ribosomal protein S20

ProteinName: 40S ribosomal protein S20 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.476479 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #13: protein, 40S ribosomal protein S25-A

ProteinName: 40S ribosomal protein S25-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.930299 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #14: protein, 40S ribosomal protein S28-A

ProteinName: 40S ribosomal protein S28-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.116281 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #15: protein, 40S ribosomal protein S29-A

ProteinName: 40S ribosomal protein S29-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.335303 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #16: protein, Ubiquitin-40S ribosomal protein S31

ProteinName: Ubiquitin-40S ribosomal protein S31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.329946 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #17: protein, Guanine nucleotide-binding protein subunit beta-like protein

ProteinName: Guanine nucleotide-binding protein subunit beta-like protein
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 34.151426 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #18: protein, 40S ribosomal protein S0-A

ProteinName: 40S ribosomal protein S0-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.811074 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #19: protein, 40S ribosomal protein S1-A

ProteinName: 40S ribosomal protein S1-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.498365 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #20: protein, 40S ribosomal protein S2

ProteinName: 40S ribosomal protein S2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.212979 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #21: protein, 40S ribosomal protein S4-A

ProteinName: 40S ribosomal protein S4-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.338133 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #22: protein, 40S ribosomal protein S6-A

ProteinName: 40S ribosomal protein S6-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.965975 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #23: protein, 40S ribosomal protein S7-A

ProteinName: 40S ribosomal protein S7-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.07157 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #24: protein, 40S ribosomal protein S8-A

ProteinName: 40S ribosomal protein S8-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.14718 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #25: protein, 40S ribosomal protein S9-A

ProteinName: 40S ribosomal protein S9-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.210662 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #26: protein, 40S ribosomal protein S11-A

ProteinName: 40S ribosomal protein S11-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.588461 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #27: protein, 40S ribosomal protein S13

ProteinName: 40S ribosomal protein S13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.928748 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #28: protein, 40S ribosomal protein S14-B

ProteinName: 40S ribosomal protein S14-BRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.560528 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #29: protein, 40S ribosomal protein S21-A

ProteinName: 40S ribosomal protein S21-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.758829 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #30: protein, 40S ribosomal protein S22-A

ProteinName: 40S ribosomal protein S22-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.518867 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #31: protein, 40S ribosomal protein S23-A

ProteinName: 40S ribosomal protein S23-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.942699 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #32: protein, 40S ribosomal protein S24-A

ProteinName: 40S ribosomal protein S24-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.23165 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #33: protein, 40S ribosomal protein S26-B

ProteinName: 40S ribosomal protein S26-BRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.022989 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #34: protein, 40S ribosomal protein S27-A

ProteinName: 40S ribosomal protein S27-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.762195 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #35: protein, 40S ribosomal protein S30-A

ProteinName: 40S ribosomal protein S30-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 5.054081 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #36: protein, Multiprotein-bridging factor 1

ProteinName: Multiprotein-bridging factor 1 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.247083 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #37: nucleic-acid, mRNA (32-MER)

nucleic acidName: mRNA (32-MER) / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
ACUAUAAAAU UUUCUUUUCU CUCUUUUUUU UU
MassTheoretical: 9.885666 kDa
SourceSpecies: Saccharomyces cerevisiae S288c (yeast)

+
Component #38: nucleic-acid, tRNA (75-MER)

nucleic acidName: tRNA (75-MER) / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AGCGCCGUGA CGCAGUGGAA GCGCGCAGGG CUCAUAACCC UGAUGUCCUC GGAUCGAAAC CGAGCGGCGC UACCA
MassTheoretical: 24.2225 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #39: nucleic-acid, tRNA (77-MER)

nucleic acidName: tRNA (77-MER) / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
CGCGGGGUGG AGCAGCCUGG UAGCUCGUCG GGCUCAUAAC CCGAAGGUCG UCGGUUCAAA UCCGGCCCCC GCAACCA
MassTheoretical: 24.802785 kDa
SourceSpecies: Saccharomyces cerevisiae S288c (yeast)

+
Component #40: nucleic-acid, 25S rRNA (3184-MER)

nucleic acidName: 25S rRNA (3184-MER) / Class: RNA / Structure: OTHER / Synthetic: No
Sequence: UUGACCUCAA AUCAGGUAGG AGUACCCGCU GAACUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACCAAC CGGGAUUGCC UUAGUAACGG CGAGUGAAGC GGCAAAAGCU CAAAUUUGAA AUCUGGUACC UUCGGUGCCC GAGUUGUAAU UUGGAGAGGG CAACUUUGGG ...Sequence:
UUGACCUCAA AUCAGGUAGG AGUACCCGCU GAACUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACCAAC CGGGAUUGCC UUAGUAACGG CGAGUGAAGC GGCAAAAGCU CAAAUUUGAA AUCUGGUACC UUCGGUGCCC GAGUUGUAAU UUGGAGAGGG CAACUUUGGG GCCGUUCCUU GUCUAUGUUC CUUGGAACAG GACGUCAUAG AGGGUGAGAA UCCCGUGUGG CGAGGAGUGC GGUUCUUUGU AAAGUGCCUU CGAAGAGUCG AGUUGUUUGG GAAUGCAGCU CUAAGUGGGU GGUAAAUUCC AUCUAAAGCU AAAUAUUGGC GAGAGACCGA UAGCGAACAA GUACAGUGAU GGAAAGAUGA AAAGAACUUU GAAAAGAGAG UGAAAAAGUA CGUGAAAUUG UUGAAAGGGA AGGGCAUUUG AUCAGACAUG GUGUUUUGUG CCCUCUGCUC CUUGUGGGUA GGGGAAUCUC GCAUUUCACU GGGCCAGCAU CAGUUUUGGU GGCAGGAUAA AUCCAUAGGA AUGUAGCUUG CCUCGGUAAG UAUUAUAGCC UGUGGGAAUA CUGCCAGCUG GGACUGAGGA CUGCGACGUA AGUCAAGGAU GCUGGCAUAA UGGUUAUAUG CCGCCCGUCU UGAAACACGG ACCAAGGAGU CUAACGUCUA UGCGAGUGUU UGGGUGUAAA ACCCAUACGC GUAAUGAAAG UGAACGUAGG UUGGGGCCUC GCAAGAGGUG CACAAUCGAC CGAUCCUGAU GUCUUCGGAU GGAUUUGAGU AAGAGCAUAG CUGUUGGGAC CCGAAAGAUG GUGAACUAUG CCUGAAUAGG GUGAAGCCAG AGGAAACUCU GGUGGAGGCU CGUAGCGGUU CUGACGUGCA AAUCGAUCGU CGAAUUUGGG UAUAGGGGCG AAAGACUAAU CGAACCAUCU AGUAGCUGGU UCCUGCCGAA GUUUCCCUCA GGAUAGCAGA AGCUCGUAUC AGUUUUAUGA GGUAAAGCGA AUGAUUAGAG GUUCCGGGGU CGAAAUGACC UUGACCUAUU CUCAAACUUU AAAUAUGUAA GAAGUCCUUG UUACUUAAUU GAACGUGGAC AUUUGAAUGA AGAGCUUUUA GUGGGCCAUU UUUGGUAAGC AGAACUGGCG AUGCGGGAUG AACCGAACGU AGAGUUAAGG UGCCGGAAUA CACGCUCAUC AGACACCACA AAAGGUGUUA GUUCAUCUAG ACAGCCGGAC GGUGGCCAUG GAAGUCGGAA UCCGCUAAGG AGUGUGUAAC AACUCACCGG CCGAAUGAAC UAGCCCUGAA AAUGGAUGGC GCUCAAGCGU GUUACCUAUA CUCUACCGUC AGGGUUGAUA UGAUGCCCUG ACGAGUAGGC AGGCGUGGAG GUCAGUGACG AAGCCUAGAC CGUAAGGUCG GGUCGAACGG CCUCUAGUGC AGAUCUUGGU GGUAGUAGCA AAUAUUCAAA UGAGAACUUU GAAGACUGAA GUGGGGAAAG GUUCCACGUC AACAGCAGUU GGACGUGGGU UAGUCGAUCC UAAGAGAUGG GGAAGCUCCG UUUCAAAGGC CUGAUUUUAU GCAGGCCACC AUCGAAAGGG AAUCCGGUUA AGAUUCCGGA ACCUGGAUAU GGAUUCUUCA CGGUAACGUA ACUGAAUGUG GAGACGUCGG CGCGAGCCCU GGGAGGAGUU AUCUUUUCUU CUUAACAGCU UAUCACCCCG GAAUUGGUUU AUCCGGAGAU GGGGUCUUAU GGCUGGAAGA GGCCAGCACC UUUGCUGGCU CCGGUGCGCU UGUGACGGCC CGUGAAAAUC CACAGGAAGG AAUAGUUUUC AUGCCAGGUC GUACUGAUAA CCGCAGCAGG UCUCCAAGGU GAACAGCCUC UAGUUGAUAG AAUAAUGUAG AUAAGGGAAG UCGGCAAAAU AGAUCCGUAA CUUCGGGAUA AGGAUUGGCU CUAAGGGUCG GGUAGUGAGG GCCUUGGUCA GACGCAGCGG GCGUGCUUGU GGACUGCUUG GUGGGGCUUG CUCUGCUAGG CGGACUACUU GCGUGCCUUG UUGUAGACGG CCUUGGUAGG UCUCUUGUAG ACCGUCGCUU GCUACAAUUA ACGAUCAACU UAGAACUGGU ACGGACAAGG GGAAUCUGAC UGUCUAAUUA AAACAUAGCA UUGCGAUGGU CAGAAAGUGA UGUUGACGCA AUGUGAUUUC UGCCCAGUGC UCUGAAUGUC AAAGUGAAGA AAUUCAACCA AGCGCGGGUA AACGGCGGGA GUAACUAUGA CUCUCUUAAG GUAGCCAAAU GCCUCGUCAU CUAAUUAGUG ACGCGCAUGA AUGGAUUAAC GAGAUUCCCA CUGUCCCUAU CUACUAUCUA GCGAAACCAC AGCCAAGGGA ACGGGCUUGG CAGAAUCAGC GGGGAAAGAA GACCCUGUUG AGCUUGACUC UAGUUUGACA UUGUGAAGAG ACAUAGAGGG UGUAGAAUAA GUGGGAGCUU CGGCGCCAGU GAAAUACCAC UACCUUUAUA GUUUCUUUAC UUAUUCAAUG AAGCGGAGCU GGAAUUCAUU UUCCACGUUC UAGCAUUCAA GGUCCCAUUC GGGGCUGAUC CGGGUUGAAG ACAUUGUCAG GUGGGGAGUU UGGCUGGGGC GGCACAUCUG UUAAACGAUA ACGCAGAUGU CCUAAGGGGG GCUCAUGGAG AACAGAAAUC UCCAGUAGAA CAAAAGGGUA AAAGCCCCCU UGAUUUUGAU UUUCAGUGUG AAUACAAACC AUGAAAGUGU GGCCUAUCGA UCCUUUAGUC CCUCGGAAUU UGAGGCUAGA GGUGCCAGAA AAGUUACCAC AGGGAUAACU GGCUUGUGGC AGUCAAGCGU UCAUAGCGAC AUUGCUUUUU GAUUCUUCGA UGUCGGCUCU UCCUAUCAUA CCGAAGCAGA AUUCGGUAAG CGUUGGAUUG UUCACCCACU AAUAGGGAAC GUGAGCUGGG UUUAGACCGU CGUGAGACAG GUUAGUUUUA CCCUACUGAU GAAUGUUACC GCAAUAGUAA UUGAACUUAG UACGAGAGGA ACAGUUCAUU CGGAUAAUUG GUUUUUGCGG CUGUCUGAUC AGGCAUUGCC GCGAAGCUAC CAUCCGCUGG AUUAUGGCUG AACGCCUCUA AGUCAGAAUC CAUGCUAGAA CGCGGUGAUU UCUUUGCUCC ACACAAUAUA GAUGGAUACG AAUAAGGCGU CCUUGUGGCG UCGCUGAACC AUAGCAGGCU AGCAACGGUG CACUUGGCGG AAAGGCCUUG GGUGCUUGCU GGCGAAUUGC AAUGUCAUUU UGCGUGGGGA UAAAUCAUUU GUAUACGACU UAGAUGUACA ACGGGGUAUU GUAAGCAGUA GAGUAGCCUU GUUGUUACGA UCUGCUGAGA UUAAGCCUUU GUUGUCUGAU UUGU
MassTheoretical: 1.096842375 MDa
SourceSpecies: Saccharomyces cerevisiae S288C (yeast)

+
Component #41: nucleic-acid, 5S rRNA (121-MER)

nucleic acidName: 5S rRNA (121-MER) / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGGU GACCAUACGC GAAACUCAGG UGCUGCAAUC U
MassTheoretical: 38.951105 kDa
SourceSpecies: Saccharomyces cerevisiae S288c (yeast)

+
Component #42: nucleic-acid, 5.8S rRNA (158-MER)

nucleic acidName: 5.8S rRNA (158-MER) / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAAU CAUCGAAUCU UUGAACGCAC AUUGCGCCCC UUGGUAUUCC AGGGGGCAUG CCUGUUUGAG CGUCAUUU
MassTheoretical: 50.682922 kDa
SourceSpecies: Saccharomyces cerevisiae S288c (yeast)

+
Component #43: protein, 60S ribosomal protein L2-A

ProteinName: 60S ribosomal protein L2-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.089158 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #44: protein, 60S ribosomal protein L3

ProteinName: 60S ribosomal protein L3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 43.719602 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #45: protein, 60S ribosomal protein L4-A

ProteinName: 60S ribosomal protein L4-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 39.027926 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #46: protein, 60S ribosomal protein L5

ProteinName: 60S ribosomal protein L5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 33.415387 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #47: protein, 60S ribosomal protein L6-B

ProteinName: 60S ribosomal protein L6-BRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.93532 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #48: protein, 60S ribosomal protein L7-A

ProteinName: 60S ribosomal protein L7-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.240377 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #49: protein, 60S ribosomal protein L8-A

ProteinName: 60S ribosomal protein L8-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.814998 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #50: protein, 60S ribosomal protein L9-A

ProteinName: 60S ribosomal protein L9-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.605061 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #51: protein, 60S ribosomal protein L10

ProteinName: 60S ribosomal protein L10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 25.080062 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #52: protein, 60S ribosomal protein L11-B

ProteinName: 60S ribosomal protein L11-BRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 19.266082 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #53: protein, 60S ribosomal protein L13-A

ProteinName: 60S ribosomal protein L13-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.885234 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #54: protein, 60S ribosomal protein L14-A

ProteinName: 60S ribosomal protein L14-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.976794 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #55: protein, 60S ribosomal protein L15-A

ProteinName: 60S ribosomal protein L15-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.351162 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #56: protein, 60S ribosomal protein L16-A

ProteinName: 60S ribosomal protein L16-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.028951 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #57: protein, 60S ribosomal protein L17-A

ProteinName: 60S ribosomal protein L17-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.458322 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #58: protein, 60S ribosomal protein L18-A

ProteinName: 60S ribosomal protein L18-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.478059 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #59: protein, 60S ribosomal protein L19-A

ProteinName: 60S ribosomal protein L19-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 21.631121 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #60: protein, 60S ribosomal protein L20-A

ProteinName: 60S ribosomal protein L20-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.347654 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #61: protein, 60S ribosomal protein L21-A

ProteinName: 60S ribosomal protein L21-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.148066 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #62: protein, 60S ribosomal protein L22-A

ProteinName: 60S ribosomal protein L22-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.263863 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #63: protein, 60S ribosomal protein L23-A

ProteinName: 60S ribosomal protein L23-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.362755 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #64: protein, 60S ribosomal protein L24-A

ProteinName: 60S ribosomal protein L24-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.214498 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #65: protein, 60S ribosomal protein L25

ProteinName: 60S ribosomal protein L25 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.771176 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #66: protein, 60S ribosomal protein L26-A

ProteinName: 60S ribosomal protein L26-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.005475 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #67: protein, 60S ribosomal protein L27-A

ProteinName: 60S ribosomal protein L27-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.437166 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #68: protein, 60S ribosomal protein L28

ProteinName: 60S ribosomal protein L28 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.630471 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #69: protein, 60S ribosomal protein L29

ProteinName: 60S ribosomal protein L29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.560688 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #70: protein, 60S ribosomal protein L30

ProteinName: 60S ribosomal protein L30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.487266 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #71: protein, 60S ribosomal protein L31-A

ProteinName: 60S ribosomal protein L31-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.649754 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #72: protein, 60S ribosomal protein L32

ProteinName: 60S ribosomal protein L32 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.478054 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #73: protein, 60S ribosomal protein L33-A

ProteinName: 60S ribosomal protein L33-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.045935 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #74: protein, 60S ribosomal protein L34-A

ProteinName: 60S ribosomal protein L34-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.608831 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #75: protein, 60S ribosomal protein L35-A

ProteinName: 60S ribosomal protein L35-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.811444 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #76: protein, 60S ribosomal protein L36-A

ProteinName: 60S ribosomal protein L36-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.020063 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #77: protein, 60S ribosomal protein L37-A

ProteinName: 60S ribosomal protein L37-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.229632 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #78: protein, 60S ribosomal protein L38

ProteinName: 60S ribosomal protein L38 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 8.714363 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #79: protein, 60S ribosomal protein L39

ProteinName: 60S ribosomal protein L39 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.227444 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #80: protein, Ubiquitin-60S ribosomal protein L40

ProteinName: Ubiquitin-60S ribosomal protein L40 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.032321 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #81: protein, 60S ribosomal protein L41-B

ProteinName: 60S ribosomal protein L41-BRibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 3.354243 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #82: protein, 60S ribosomal protein L42-A

ProteinName: 60S ribosomal protein L42-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.840159 kDa
SourceSpecies: Baker's yeast (baker's yeast)

+
Component #83: protein, 60S ribosomal protein L43-A

ProteinName: 60S ribosomal protein L43-ARibosome / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.981756 kDa
SourceSpecies: Baker's yeast (baker's yeast)

-
Experimental details

-
Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 7.5
VitrificationCryogen name: OTHER

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 2.5 e/Å2 / Illumination mode: SPOT SCAN
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: FEI FALCON II (4k x 4k)

-
Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 30016
3D reconstructionSoftware: RELION / Resolution: 4.36 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot (resolution estimation)

-
Atomic model buiding

Modeling #1Refinement protocol: rigid body / Refinement space: REAL
Output model

+
About Yorodumi

-
News

-
Aug 12, 2020. New: Covid-19 info

New: Covid-19 info

  • New page: Covid-19 featured information page in EM Navigator

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

-
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. New: Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242):Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force. (see PDBe EMDB page)
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is "EMD"? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB at PDBe / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary. This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated. See below links for details.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software). Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

+
Jun 16, 2017. Omokage search with filter

Omokage search with filter

  • Result of Omokage search can be filtered by keywords and the database types

Related info.:Omokage search

Read more

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more