EMDB-12535: Structure of the yeast Gcn1 bound to a colliding stalled 80S ribo...

Basic information

• Entry: Database: EMDB / ID: EMD-12535
• Title: Structure of the yeast Gcn1 bound to a colliding stalled 80S ribosome with MBF1, A/P-tRNA and P/E-tRNA
• Map data: map colliding ribosome

Sample

• Complex: Structure of the yeast Gcn1-bound coilliding stalled 80S ribosome with MBF1, Gir2, A/P-tRNA and P/E-tRNA
  • RNA: x 7 types
  • Protein or peptide: x 75 types

• Keywords: Ribosome / Disome / GCN1 / Translation / GAAC / MBF1

Function / homology

Function:

GCN2-mediated signaling / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / Negative regulators of DDX58/IFIH1 signaling / regulation of amino acid metabolic process / negative regulation of glucose mediated signaling pathway / positive regulation of translational fidelity / RMTs methylate histone arginines / Protein methylation / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / regulation of polysaccharide biosynthetic process / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / nonfunctional rRNA decay / transporter complex / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / response to cycloheximide / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA destabilization / lipopolysaccharide transport / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of translational frameshifting / Formation of a pool of free 40S subunits / positive regulation of protein kinase activity / preribosome, large subunit precursor / L13a-mediated translational silencing of Ceruloplasmin expression / Gram-negative-bacterium-type cell outer membrane assembly / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translational elongation / ribosomal large subunit export from nucleus / G-protein alpha-subunit binding / 90S preribosome / Ub-specific processing proteases / ribosomal subunit export from nucleus / regulation of translational fidelity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / translational termination / maturation of LSU-rRNA / ribosomal small subunit export from nucleus / translation regulator activity / DNA-(apurinic or apyrimidinic site) endonuclease activity / rescue of stalled ribosome / cellular response to amino acid starvation / protein kinase C binding / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome assembly / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / macroautophagy / translational initiation / small-subunit processome / modification-dependent protein catabolic process / protein tag activity / maintenance of translational fidelity / cell outer membrane / cytoplasmic stress granule / rRNA processing / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / small ribosomal subunit rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / protein ubiquitination / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / G protein-coupled receptor signaling pathway / translation / negative regulation of gene expression / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / nucleolus / mitochondrion / DNA binding / RNA binding / zinc ion binding / nucleoplasm / nucleus / cytosol / cytoplasm

Domain/homology:

Multiprotein bridging factor 1, N-terminal / Multiprotein bridging factor 1 / Helix-turn-helix / Helix-turn-helix XRE-family like proteins / Cro/C1-type HTH domain profile. / Cro/C1-type helix-turn-helix domain / LptD, C-terminal / LPS-assembly protein LptD / : / LPS transport system D / Organic solvent tolerance-like, N-terminal / LptA/(LptD N-terminal domain) LPS transport protein / Group II dsDNA virus coat/capsid protein / Lambda repressor-like, DNA-binding domain superfamily / : / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein S5, eukaryotic/archaeal / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / S27a-like superfamily / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L10e / 40S Ribosomal protein S10 / : / Ribosomal protein L44e signature. / Ribosomal protein S7e signature. / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19, eukaryotic / Ribosomal protein L19e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / : / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L6e signature. / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / : / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein L44e / Ribosomal protein S3Ae, conserved site / Ribosomal protein L44 / Ribosomal protein S3Ae signature. / Ribosomal protein S30 / Ribosomal protein S30 / Ribosomal protein S27a / Ribosomal protein L34e, conserved site / Ribosomal protein S27a / Ribosomal protein L34e signature. / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein L5 eukaryotic, C-terminal / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal L18 C-terminal region / Ribosomal L40e family / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L23/L25, N-terminal / : / Ribosomal protein L23, N-terminal domain / Ribosomal protein S27e signature. / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S4e, N-terminal, conserved site

Component:

Small ribosomal subunit protein uS4A / Multiprotein-bridging factor 1 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein eL6B / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS15 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein eS21A / Small ribosomal subunit protein uS8A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS30A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL2A / Small ribosomal subunit protein uS17A / Large ribosomal subunit protein eL18A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Small ribosomal subunit protein eS32B / Large ribosomal subunit protein uL4A / Putative major capsid protein p72 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein eS17B / Large ribosomal subunit protein eL8A / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL13A / Small ribosomal subunit protein eS7A / Small ribosomal subunit protein uS2A / Small ribosomal subunit protein eS1A / Small ribosomal subunit protein eS27A / Large ribosomal subunit protein eL14A / Small ribosomal subunit protein RACK1 / Large ribosomal subunit protein eL32 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS11B / Small ribosomal subunit protein eS26B / Small ribosomal subunit protein uS14A / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL34A / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL21A / Small ribosomal subunit protein eS10A / LPS-assembly protein LptD / Large ribosomal subunit protein uL5B / Small ribosomal subunit protein eS25A / Small ribosomal subunit protein eS28A

• Biological species: Saccharomyces cerevisiae S288c (yeast) / Saccharomyces cerevisiae S288C (yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 4.36 Å
• Authors: Pochopien AA / Beckert B

Funding support

Germany, 2 items

Organization	Grant number	Country
German Research Foundation (DFG)	WI3285/8-1	Germany
German Research Foundation (DFG)	SPP-1879	Germany

• Citation: Journal: Proc Natl Acad Sci U S A / Year: 2021; Title: Structure of Gcn1 bound to stalled and colliding 80S ribosomes.; Authors: Agnieszka A Pochopien / Bertrand Beckert / Sergo Kasvandik / Otto Berninghausen / Roland Beckmann / Tanel Tenson / Daniel N Wilson / ; Abstract: The Gcn pathway is conserved in all eukaryotes, including mammals such as humans, where it is a crucial part of the integrated stress response (ISR). Gcn1 serves as an essential effector protein for the kinase Gcn2, which in turn is activated by stalled ribosomes, leading to phosphorylation of eIF2 and a subsequent global repression of translation. The fine-tuning of this adaptive response is performed by the Rbg2/Gir2 complex, a negative regulator of Gcn2. Despite the wealth of available biochemical data, information on structures of Gcn proteins on the ribosome has remained elusive. Here we present a cryo-electron microscopy structure of the yeast Gcn1 protein in complex with stalled and colliding 80S ribosomes. Gcn1 interacts with both 80S ribosomes within the disome, such that the Gcn1 HEAT repeats span from the P-stalk region on the colliding ribosome to the P-stalk and the A-site region of the lead ribosome. The lead ribosome is stalled in a nonrotated state with peptidyl-tRNA in the A-site, uncharged tRNA in the P-site, eIF5A in the E-site, and Rbg2/Gir2 in the A-site factor binding region. By contrast, the colliding ribosome adopts a rotated state with peptidyl-tRNA in a hybrid A/P-site, uncharged-tRNA in the P/E-site, and Mbf1 bound adjacent to the mRNA entry channel on the 40S subunit. Collectively, our findings reveal the interaction mode of the Gcn2-activating protein Gcn1 with colliding ribosomes and provide insight into the regulation of Gcn2 activation. The binding of Gcn1 to a disome has important implications not only for the Gcn2-activated ISR, but also for the general ribosome-associated quality control pathways.

History

Deposition	Mar 3, 2021	-
Header (metadata) release	Apr 14, 2021	-
Map release	Apr 14, 2021	-
Update	Jul 9, 2025	-
Current status	Jul 9, 2025	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_12535.map.gz / Format: CCP4 / Size: 1.3 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: map colliding ribosome
• Voxel size: X=Y=Z: 1.084 Å

Density

Contour Level	By AUTHOR: 0.03 / Movie #1: 0.03
Minimum - Maximum	-0.31267092 - 0.5077412
Average (Standard dev.)	0.0007687183 (±0.0064846477)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 700 700 700 Spacing 700 700 700
Cell	A=B=C: 758.8 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.084	1.084	1.084
M x/y/z	700	700	700
origin x/y/z	0.000	0.000	0.000
length x/y/z	758.800	758.800	758.800
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	700	700	700
D min/max/mean	-0.313	0.508	0.001

Supplemental data

Additional map: map colliding ribosome relion final run

• File: emd_12535_additional_1.map
• Annotation: map colliding ribosome relion final run

Additional map: focus refinement 40S only and postprocess

• File: emd_12535_additional_2.map
• Annotation: focus refinement 40S only and postprocess

Additional map: map colliding ribosome postprocess

• File: emd_12535_additional_3.map
• Annotation: map colliding ribosome postprocess

Half map: Half map colliding ribosome

• File: emd_12535_half_map_1.map
• Annotation: Half map colliding ribosome

Half map: Half map colliding ribosome

• File: emd_12535_half_map_2.map
• Annotation: Half map colliding ribosome

Sample components

Entire : Structure of the yeast Gcn1-bound coilliding stalled 80S ribosome...

• Entire: Name: Structure of the yeast Gcn1-bound coilliding stalled 80S ribosome with MBF1, Gir2, A/P-tRNA and P/E-tRNA

Components

• Complex: Structure of the yeast Gcn1-bound coilliding stalled 80S ribosome with MBF1, Gir2, A/P-tRNA and P/E-tRNA
  • RNA: TPA_inf: Saccharomyces cerevisiae S288C chromosome XII, complete sequence
  • Protein or peptide: 40S ribosomal protein S3
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S10-A
  • Protein or peptide: 40S ribosomal protein S12
  • Protein or peptide: 40S ribosomal protein S15
  • Protein or peptide: 40S ribosomal protein S16-A
  • Protein or peptide: 40S ribosomal protein S17-B
  • Protein or peptide: 40S ribosomal protein S18-A
  • Protein or peptide: 40S ribosomal protein S19-A
  • Protein or peptide: 40S ribosomal protein S20
  • Protein or peptide: 40S ribosomal protein S25-A
  • Protein or peptide: 40S ribosomal protein S28-A
  • Protein or peptide: 40S ribosomal protein S29-A
  • Protein or peptide: Ubiquitin-40S ribosomal protein S31
  • Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
  • Protein or peptide: 40S ribosomal protein S0-A
  • Protein or peptide: 40S ribosomal protein S1-A
  • Protein or peptide: 40S ribosomal protein S2
  • Protein or peptide: 40S ribosomal protein S4-A
  • Protein or peptide: 40S ribosomal protein S6-A
  • Protein or peptide: 40S ribosomal protein S7-A
  • Protein or peptide: 40S ribosomal protein S8-A
  • Protein or peptide: 40S ribosomal protein S9-A
  • Protein or peptide: 40S ribosomal protein S11-A
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: 40S ribosomal protein S14-B
  • Protein or peptide: 40S ribosomal protein S21-A
  • Protein or peptide: 40S ribosomal protein S22-A
  • Protein or peptide: 40S ribosomal protein S23-A
  • Protein or peptide: 40S ribosomal protein S24-A
  • Protein or peptide: 40S ribosomal protein S26-B
  • Protein or peptide: 40S ribosomal protein S27-A
  • Protein or peptide: 40S ribosomal protein S30-A
  • Protein or peptide: Multiprotein-bridging factor 1
  • RNA: mRNA (32-MER)
  • RNA: tRNA (75-MER)
  • RNA: tRNA (77-MER)
  • RNA: 25S rRNA (3184-MER)
  • RNA: 5S rRNA (121-MER)
  • RNA: 5.8S rRNA (158-MER)
  • Protein or peptide: 60S ribosomal protein L2-A
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: 60S ribosomal protein L4-A
  • Protein or peptide: 60S ribosomal protein L5
  • Protein or peptide: 60S ribosomal protein L6-B
  • Protein or peptide: 60S ribosomal protein L7-A
  • Protein or peptide: 60S ribosomal protein L8-A
  • Protein or peptide: 60S ribosomal protein L9-A
  • Protein or peptide: 60S ribosomal protein L10
  • Protein or peptide: 60S ribosomal protein L11-B
  • Protein or peptide: 60S ribosomal protein L13-A
  • Protein or peptide: 60S ribosomal protein L14-A
  • Protein or peptide: 60S ribosomal protein L15-A
  • Protein or peptide: 60S ribosomal protein L16-A
  • Protein or peptide: 60S ribosomal protein L17-A
  • Protein or peptide: 60S ribosomal protein L18-A
  • Protein or peptide: 60S ribosomal protein L19-A
  • Protein or peptide: 60S ribosomal protein L20-A
  • Protein or peptide: 60S ribosomal protein L21-A
  • Protein or peptide: 60S ribosomal protein L22-A
  • Protein or peptide: 60S ribosomal protein L23-A
  • Protein or peptide: 60S ribosomal protein L24-A
  • Protein or peptide: 60S ribosomal protein L25
  • Protein or peptide: 60S ribosomal protein L26-A
  • Protein or peptide: 60S ribosomal protein L27-A
  • Protein or peptide: 60S ribosomal protein L28
  • Protein or peptide: 60S ribosomal protein L29
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L31-A
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: 60S ribosomal protein L33-A
  • Protein or peptide: 60S ribosomal protein L34-A
  • Protein or peptide: 60S ribosomal protein L35-A
  • Protein or peptide: 60S ribosomal protein L36-A
  • Protein or peptide: 60S ribosomal protein L37-A
  • Protein or peptide: 60S ribosomal protein L38
  • Protein or peptide: 60S ribosomal protein L39
  • Protein or peptide: Ubiquitin-60S ribosomal protein L40
  • Protein or peptide: 60S ribosomal protein L41-B
  • Protein or peptide: 60S ribosomal protein L42-A
  • Protein or peptide: 60S ribosomal protein L43-A

Supramolecule #1: Structure of the yeast Gcn1-bound coilliding stalled 80S ribosome...

• Supramolecule: Name: Structure of the yeast Gcn1-bound coilliding stalled 80S ribosome with MBF1, Gir2, A/P-tRNA and P/E-tRNA; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#82
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)

Macromolecule #1: TPA_inf: Saccharomyces cerevisiae S288C chromosome XII, complete ...

• Macromolecule: Name: TPA_inf: Saccharomyces cerevisiae S288C chromosome XII, complete sequence; type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 579.761938 KDa

Sequence

String:

UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA AUACAUGCUU AAAAUCUCGA CCCUUUGGAA GAGAUGUAUU UAUUAGAUAA AAAAUCAAUG UCUUCGGACU CU UUGAUGA UUCAUAAUAA CUUUUCGAAU CGCAUGGCCU UGUGCUGGCG AUGGUUCAUU CAAAUUUCUG CCCUAUCAAC UUU CGAUGG UAGGAUAGUG GCCUACCAUG GUUUCAACGG GUAACGGGGA AUAAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAA ACGGC UACCACAUCC AAGGAAGGCA GCAGGCGCGC AAAUUACCCA AUCCUAAUUC AGGGAGGUAG UGACAAUAAA UAACG AUAC AGGGCCCAUU CGGGUCUUGU AAUUGGAAUG AGUACAAUGU AAAUACCUUA ACGAGGAACA AUUGGAGGGC AAGUCU GGU GCCAGCAGCC GCGGUAAUUC CAGCUCCAAU AGCGUAUAUU AAAGUUGUUG CAGUUAAAAA GCUCGUAGUU GAACUUU GG GCCCGGUUGG CCGGUCCGAU UUUUUCGUGU ACUGGAUUUC CAACGGGGCC UUUCCUUCUG GCUAACCUUG AGUCCUUG U GGCUCUUGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUGUUCAAA GCAGGCGUAU UGCUCGAAUA UAUUAGCAU GGAAUAAUAG AAUAGGACGU UUGGUUCUAU UUUGUUGGUU UCUAGGACCA UCGUAAUGAU UAAUAGGGAC GGUCGGGGGC AUCAGUAUU CAAUUGUCAG AGGUGAAAUU CUUGGAUUUA UUGAAGACUA ACUACUGCGA AAGCAUUUGC CAAGGACGUU U UCAUUAAU CAAGAACGAA AGUUAGGGGA UCGAAGAUGA UCAGAUACCG UCGUAGUCUU AACCAUAAAC UAUGCCGACU AG GGAUCGG GUGGUGUUUU UUUAAUGACC CACUCGGCAC CUUACGAGAA AUCAAAGUCU UUGGGUUCUG GGGGGAGUAU GGU CGCAAG GCUGAAACUU AAAGGAAUUG ACGGAAGGGC ACCACCAGGA GUGGAGCCUG CGGCUUAAUU UGACUCAACA CGGG GAAAC UCACCAGGUC CAGACACAAU AAGGAUUGAC AGAUUGAGAG CUCUUUCUUG AUUUUGUGGG UGGUGGUGCA UGGCC GUUC UUAGUUGGUG GAGUGAUUUG UCUGCUUAAU UGCGAUAACG AACGAGACCU UAACCUACUA AAUAGUGGUG CUAGCA UUU GCUGGUUAUC CACUUCUUAG AGGGACUAUC GGUUUCAAGC CGAUGGAAGU UUGAGGCAAU AACAGGUCUG UGAUGCC CU UAGACGUUCU GGGCCGCACG CGCGCUACAC UGACGGAGCC AGCGAGUCUA ACCUUGGCCG AGAGGUCUUG GUAAUCUU G UGAAACUCCG UCGUGCUGGG GAUAGAGCAU UGUAAUUAUU GCUCUUCAAC GAGGAAUUCC UAGUAAGCGC AAGUCAUCA GCUUGCGUUG AUUACGUCCC UGCCCUUUGU ACACACCGCC CGUCGCUAGU ACCGAUUGAA UGGCUUAGUG AGGCCUCAGG AUCUGCUUA GAGAAGGGGG CAACUCCAUC UCAGAGCGGA GAAUUUGGAC AAACUUGGUC AUUUAGAGGA ACUAAAAGUC G UAACAAGG UUUCCGUAGG UGAACCUGCG GAAGGAUCAU UA

GENBANK: GENBANK: BK006945.2

Macromolecule #36: mRNA (32-MER)

• Macromolecule: Name: mRNA (32-MER) / type: rna / ID: 36 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 9.885666 KDa

Sequence

String:

ACUAUAAAAU UUUCUUUUCU CUCUUUUUUU UU

Macromolecule #37: tRNA (75-MER)

• Macromolecule: Name: tRNA (75-MER) / type: rna / ID: 37 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 24.2225 KDa

Sequence

String:

AGCGCCGUGA CGCAGUGGAA GCGCGCAGGG CUCAUAACCC UGAUGUCCUC GGAUCGAAAC CGAGCGGCGC UACCA

GENBANK: GENBANK: K00321.1

Macromolecule #38: tRNA (77-MER)

• Macromolecule: Name: tRNA (77-MER) / type: rna / ID: 38 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 24.802785 KDa

Sequence

String:

CGCGGGGUGG AGCAGCCUGG UAGCUCGUCG GGCUCAUAAC CCGAAGGUCG UCGGUUCAAA UCCGGCCCCC GCAACCA

Macromolecule #39: 25S rRNA (3184-MER)

• Macromolecule: Name: 25S rRNA (3184-MER) / type: rna / ID: 39 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 1.096842375 MDa

Sequence

String:

UUGACCUCAA AUCAGGUAGG AGUACCCGCU GAACUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACCAAC CGGGAUUGCC UUAGUAACG GCGAGUGAAG CGGCAAAAGC UCAAAUUUGA AAUCUGGUAC CUUCGGUGCC CGAGUUGUAA UUUGGAGAGG G CAACUUUG GGGCCGUUCC UUGUCUAUGU UCCUUGGAAC AGGACGUCAU AGAGGGUGAG AAUCCCGUGU GGCGAGGAGU GC GGUUCUU UGUAAAGUGC CUUCGAAGAG UCGAGUUGUU UGGGAAUGCA GCUCUAAGUG GGUGGUAAAU UCCAUCUAAA GCU AAAUAU UGGCGAGAGA CCGAUAGCGA ACAAGUACAG UGAUGGAAAG AUGAAAAGAA CUUUGAAAAG AGAGUGAAAA AGUA CGUGA AAUUGUUGAA AGGGAAGGGC AUUUGAUCAG ACAUGGUGUU UUGUGCCCUC UGCUCCUUGU GGGUAGGGGA AUCUC GCAU UUCACUGGGC CAGCAUCAGU UUUGGUGGCA GGAUAAAUCC AUAGGAAUGU AGCUUGCCUC GGUAAGUAUU AUAGCC UGU GGGAAUACUG CCAGCUGGGA CUGAGGACUG CGACGUAAGU CAAGGAUGCU GGCAUAAUGG UUAUAUGCCG CCCGUCU UG AAACACGGAC CAAGGAGUCU AACGUCUAUG CGAGUGUUUG GGUGUAAAAC CCAUACGCGU AAUGAAAGUG AACGUAGG U UGGGGCCUCG CAAGAGGUGC ACAAUCGACC GAUCCUGAUG UCUUCGGAUG GAUUUGAGUA AGAGCAUAGC UGUUGGGAC CCGAAAGAUG GUGAACUAUG CCUGAAUAGG GUGAAGCCAG AGGAAACUCU GGUGGAGGCU CGUAGCGGUU CUGACGUGCA AAUCGAUCG UCGAAUUUGG GUAUAGGGGC GAAAGACUAA UCGAACCAUC UAGUAGCUGG UUCCUGCCGA AGUUUCCCUC A GGAUAGCA GAAGCUCGUA UCAGUUUUAU GAGGUAAAGC GAAUGAUUAG AGGUUCCGGG GUCGAAAUGA CCUUGACCUA UU CUCAAAC UUUAAAUAUG UAAGAAGUCC UUGUUACUUA AUUGAACGUG GACAUUUGAA UGAAGAGCUU UUAGUGGGCC AUU UUUGGU AAGCAGAACU GGCGAUGCGG GAUGAACCGA ACGUAGAGUU AAGGUGCCGG AAUACACGCU CAUCAGACAC CACA AAAGG UGUUAGUUCA UCUAGACAGC CGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAGGAGUGU GUAACAACUC ACCGG CCGA AUGAACUAGC CCUGAAAAUG GAUGGCGCUC AAGCGUGUUA CCUAUACUCU ACCGUCAGGG UUGAUAUGAU GCCCUG ACG AGUAGGCAGG CGUGGAGGUC AGUGACGAAG CCUAGACCGU AAGGUCGGGU CGAACGGCCU CUAGUGCAGA UCUUGGU GG UAGUAGCAAA UAUUCAAAUG AGAACUUUGA AGACUGAAGU GGGGAAAGGU UCCACGUCAA CAGCAGUUGG ACGUGGGU U AGUCGAUCCU AAGAGAUGGG GAAGCUCCGU UUCAAAGGCC UGAUUUUAUG CAGGCCACCA UCGAAAGGGA AUCCGGUUA AGAUUCCGGA ACCUGGAUAU GGAUUCUUCA CGGUAACGUA ACUGAAUGUG GAGACGUCGG CGCGAGCCCU GGGAGGAGUU AUCUUUUCU UCUUAACAGC UUAUCACCCC GGAAUUGGUU UAUCCGGAGA UGGGGUCUUA UGGCUGGAAG AGGCCAGCAC C UUUGCUGG CUCCGGUGCG CUUGUGACGG CCCGUGAAAA UCCACAGGAA GGAAUAGUUU UCAUGCCAGG UCGUACUGAU AA CCGCAGC AGGUCUCCAA GGUGAACAGC CUCUAGUUGA UAGAAUAAUG UAGAUAAGGG AAGUCGGCAA AAUAGAUCCG UAA CUUCGG GAUAAGGAUU GGCUCUAAGG GUCGGGUAGU GAGGGCCUUG GUCAGACGCA GCGGGCGUGC UUGUGGACUG CUUG GUGGG GCUUGCUCUG CUAGGCGGAC UACUUGCGUG CCUUGUUGUA GACGGCCUUG GUAGGUCUCU UGUAGACCGU CGCUU GCUA CAAUUAACGA UCAACUUAGA ACUGGUACGG ACAAGGGGAA UCUGACUGUC UAAUUAAAAC AUAGCAUUGC GAUGGU CAG AAAGUGAUGU UGACGCAAUG UGAUUUCUGC CCAGUGCUCU GAAUGUCAAA GUGAAGAAAU UCAACCAAGC GCGGGUA AA CGGCGGGAGU AACUAUGACU CUCUUAAGGU AGCCAAAUGC CUCGUCAUCU AAUUAGUGAC GCGCAUGAAU GGAUUAAC G AGAUUCCCAC UGUCCCUAUC UACUAUCUAG CGAAACCACA GCCAAGGGAA CGGGCUUGGC AGAAUCAGCG GGGAAAGAA GACCCUGUUG AGCUUGACUC UAGUUUGACA UUGUGAAGAG ACAUAGAGGG UGUAGAAUAA GUGGGAGCUU CGGCGCCAGU GAAAUACCA CUACCUUUAU AGUUUCUUUA CUUAUUCAAU GAAGCGGAGC UGGAAUUCAU UUUCCACGUU CUAGCAUUCA A GGUCCCAU UCGGGGCUGA UCCGGGUUGA AGACAUUGUC AGGUGGGGAG UUUGGCUGGG GCGGCACAUC UGUUAAACGA UA ACGCAGA UGUCCUAAGG GGGGCUCAUG GAGAACAGAA AUCUCCAGUA GAACAAAAGG GUAAAAGCCC CCUUGAUUUU GAU UUUCAG UGUGAAUACA AACCAUGAAA GUGUGGCCUA UCGAUCCUUU AGUCCCUCGG AAUUUGAGGC UAGAGGUGCC AGAA AAGUU ACCACAGGGA UAACUGGCUU GUGGCAGUCA AGCGUUCAUA GCGACAUUGC UUUUUGAUUC UUCGAUGUCG GCUCU UCCU AUCAUACCGA AGCAGAAUUC GGUAAGCGUU GGAUUGUUCA CCCACUAAUA GGGAACGUGA GCUGGGUUUA GACCGU CGU GAGACAGGUU AGUUUUACCC UACUGAUGAA UGUUACCGCA AUAGUAAUUG AACUUAGUAC GAGAGGAACA GUUCAUU CG GAUAAUUGGU UUUUGCGGCU GUCUGAUCAG GCAUUGCCGC GAAGCUACCA UCCGCUGGAU UAUGGCUGAA CGCCUCUA A GUCAGAAUCC AUGCUAGAAC GCGGUGAUUU CUUUGCUCCA CACAAUAUAG AUGGAUACGA AUAAGGCGUC CUUGUGGCG UCGCUGAACC AUAGCAGGCU AGCAACGGUG CACUUGGCGG AAAGGCCUUG GGUGCUUGCU GGCGAAUUGC AAUGUCAUUU UGCGUGGGG AUAAAUCAUU UGUAUACGAC UUAGAUGUAC AACGGGGUAU UGUAAGCAGU AGAGUAGCCU UGUUGUUACG A UCUGCUGA GAUUAAGCCU UUGUUGUCUG AUUUGU

GENBANK: GENBANK: NR_132207.1

Macromolecule #40: 5S rRNA (121-MER)

• Macromolecule: Name: 5S rRNA (121-MER) / type: rna / ID: 40 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 38.951105 KDa

Sequence

String:

GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

GENBANK: GENBANK: CP011821.1

Macromolecule #41: 5.8S rRNA (158-MER)

• Macromolecule: Name: 5.8S rRNA (158-MER) / type: rna / ID: 41 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 50.682922 KDa

Sequence

String:

AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

GENBANK: GENBANK: KT175188.1

Macromolecule #2: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 24.702791 KDa

Sequence

String:

ALISKKRKLV ADGVFYAELN EFFTRELAEE GYSGVEVRVT PTKTEVIIRA TRTQDVLGEN GRRINELTLL VQKRFKYAPG TIVLYAERV QDRGLSAVAQ AESMKFKLLN GLAIRRAAYG VVRYVMESGA KGCEVVVSGK LRAARAKAMK FADGFLIHSG Q PVNDFIDT ATRHVLMRQG VLGIKVKIMR DPAKSRTGPK ALPDAVTIIE PKEEEPILAP SVKDY

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #3: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 22.908338 KDa

Sequence

String:

FTPVVLATPI PEEVQQAQTE IKLFNKWSFE EVEVKDASLV DYVQVRQPIF VAHTAGRYAN KRFRKAQCPI IERLTNSLMM NGRNNGKKL KAVRIIKHTL DIINVLTDQN PIQVVVDAIT NTGPREDTTR VGGGGAARRQ AVDVSPLRRV NQAIALLTIG A REAAFRNI KTIAETLAEE LINAAKGSST SYAIKKKDEL ERVAKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #4: 40S ribosomal protein S10-A

• Macromolecule: Name: 40S ribosomal protein S10-A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 11.042562 KDa

Sequence

String:

MLMPKEDRNK IHQYLFQEGV VVAKKDFNQA KHEEIDTKNL YVIKALQSLT SKGYVKTQFS WQYYYYTLTE EGVEYLREYL NLPEHIVPG TYI

UniProtKB: Small ribosomal subunit protein eS10A

Macromolecule #5: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 13.327331 KDa

Sequence

String:

AEVTIEDALK VVLRTALVHD GLARGLREST KALTRGEALL VVLVSSVTEA NIIKLVEGLA NDPENKVPLI KVADAKQLGE WAGLGKIDR EGNARKVVGA SVVVVKNWGA ETDELSMIME HFSQQ

UniProtKB: Small ribosomal subunit protein eS12

Macromolecule #6: 40S ribosomal protein S15

• Macromolecule: Name: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 13.421732 KDa

Sequence

String:

THSYRGVDLE KLLEMSTEDF VKLAPARVRR RFARGMTSKP AGFMKKLRAA KLAAPENEKP APVRTHMRNM IIVPEMIGSV VGIYNGKAF NQVEIRPEML GHYLGEFSIT YTPVRHGRAG

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #7: 40S ribosomal protein S16-A

• Macromolecule: Name: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 15.659216 KDa

Sequence

String:

AVPSVQTFGK KKSATAVAHV KAGKGLIKVN GSPITLVEPE ILRFKVYEPL LLVGLDKFSN IDIRVRVTGG GHVSQVYAIR QAIAKGLVA YHQKYVDEQS KNELKKAFTS YDRTLLIADS RRPEPKKFGG KGARSRFQKS YR

UniProtKB: Small ribosomal subunit protein uS9A

Macromolecule #8: 40S ribosomal protein S17-B

• Macromolecule: Name: 40S ribosomal protein S17-B / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 14.268545 KDa

Sequence

String:

GRVRTKTVKR ASKALIERYY PKLTLDFQTN KRLCDEIATI QSKRLRNKIA GYTTHLMKRI QKGPVRGISF KLQEEERERK DQYVPEVSA LDLSRSNGVL NVDNQTSDLV KSLGLKLPLS VINVSA

UniProtKB: Small ribosomal subunit protein eS17B

Macromolecule #9: 40S ribosomal protein S18-A

• Macromolecule: Name: 40S ribosomal protein S18-A / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 16.940443 KDa

Sequence

String:

SLVVQEQGSF QHILRLLNTN VDGNIKIVYA LTTIKGVGRR YSNLVCKKAD VDLHKRAGEL TQEELERIVQ IMQNPTHYKI PAWFLNRQN DITDGKDYHT LANNVESKLR DDLERLKKIR AHRGIRHFWG LRVRGQHTKT TGRRRA

UniProtKB: Small ribosomal subunit protein uS13A

Macromolecule #10: 40S ribosomal protein S19-A

• Macromolecule: Name: 40S ribosomal protein S19-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 15.81093 KDa

Sequence

String:

PGVSVRDVAA QDFINAYASF LQRQGKLEVP GYVDIVKTSS GNEMPPQDAE GWFYKRAASV ARHIYMRKQV GVGKLNKLYG GAKSRGVRP YKHIDASGSI NRKVLQALEK IGIVEISPKG GRRISENGQR DLDRIAAQTL EEDE

UniProtKB: Small ribosomal subunit protein eS19A

Macromolecule #11: 40S ribosomal protein S20

• Macromolecule: Name: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 11.476479 KDa

Sequence

String:

IIKIRITLTS TKVKQLENVS SNIVKNAEQH NLVKKGPVRL PTKVLKISTR KTPNGEGSKT WETYEMRIHK RYIDLEAPVQ IVKRITQIT IEPGVDVEVV VA

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #12: 40S ribosomal protein S25-A

• Macromolecule: Name: 40S ribosomal protein S25-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 7.930299 KDa

Sequence

String:

QHAVILDQEK YDRILKEVPT YRYVSVSVLV DRLKIGGSLA RIALRHLEKE GIIKPISKHS KQAIYTRAT

UniProtKB: Small ribosomal subunit protein eS25A

Macromolecule #13: 40S ribosomal protein S28-A

• Macromolecule: Name: 40S ribosomal protein S28-A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 7.116281 KDa

Sequence

String:

TPVTLAKVIK VLGRTGSRGG VTQVRVEFLE DTSRTIVRNV KGPVRENDIL VLMESEREAR RLR

UniProtKB: Small ribosomal subunit protein eS28A

Macromolecule #14: 40S ribosomal protein S29-A

• Macromolecule: Name: 40S ribosomal protein S29-A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 6.335303 KDa

Sequence

String:

ENVWFSHPRR YGKGSRQCRV CSSHTGLIRK YGLNICRQCF REKANDIGFN KFR

UniProtKB: Small ribosomal subunit protein uS14A

Macromolecule #15: Ubiquitin-40S ribosomal protein S31

• Macromolecule: Name: Ubiquitin-40S ribosomal protein S31 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 8.329946 KDa

Sequence

String:

RKKKVYTTPK KIKHKHKAVK LAVLSYYKVD AEGKVTKLRR ECSNPTCGAG VFLANHKDRL YCGKCHSVYK VNA

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

Macromolecule #16: Guanine nucleotide-binding protein subunit beta-like protein

• Macromolecule: Name: Guanine nucleotide-binding protein subunit beta-like protein; type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 34.151426 KDa

Sequence

String:

LVLRGTLEGH NGWVTSLATS AGQPNLLLSA SRDKTLISWK LTGDDQKFGV PVRSFKGHSH IVQDCTLTAD GAYALSASWD KTLRLWDVA TGETYQRFVG HKSDVMSVDI DKKASMIISG SRDKTIKVWT IKGQCLATLL GHNDWVSQVR VVPNEAADDD S VTIISAGN DKMVKAWNLN QFQIEADFIG HNSNINTLTA SPDGTLIASA GKDGEIMLWN LAAKKAMYTL SAQDEVFSLA FS PNRYWLA AATATGIKVF SLDPQYLVDD LRPEFAGYSK AAEPHAVSLA WSADGQTLFA GYTDNVIRVW QVMTAN

UniProtKB: Small ribosomal subunit protein RACK1

Macromolecule #17: 40S ribosomal protein S0-A

• Macromolecule: Name: 40S ribosomal protein S0-A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 22.811074 KDa

Sequence

String:

SLPATFDLTP EDAQLLLAAN THLGARNVQV HQEPYVFNAR PDGVHVINVG KTWEKLVLAA RIIAAIPNPE DVVAISSRTF GQRAVLKFA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKEASYVN IPVIALTDLD SPSEFVDVAI P CNNRGKHS IGLIWYLLAR EVLRLRGALV DRTQPWSIMP DLYFYRDP

UniProtKB: Small ribosomal subunit protein uS2A

Macromolecule #18: 40S ribosomal protein S1-A

• Macromolecule: Name: 40S ribosomal protein S1-A / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 24.498365 KDa

Sequence

String:

VVDPFTRKEW FDIKAPSTFE NRNVGKTLVN KSTGLKSASD ALKGRVVEVC LADLQGSEDH SFRKIKLRVD EVQGKNLLTN FHGMDFTTD KLRSMVRKWQ TLIEANVTVK TSDDYVLRIF AIAFTRKQAN QVKRHSYAQS SHIRAIRKVI SEILTKEVQG S TLAQLTSK LIPEVINKEI ENATKDIFPL QNIHVRKVKL LKQPKFDVGA LMALHGEG

UniProtKB: Small ribosomal subunit protein eS1A

Macromolecule #19: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 23.212979 KDa

Sequence

String:

GWVPVTKLGR LVKAGKITTI EEIFLHSLPV KEFQIIDTLL PGLQDEVMNI KPVQKQTRAG QRTRFKAVVV VGDSNGHVGL GIKTAKEVA GAIRAGIIIA KLSVIPIRRG YWGTNLGQPH SLATKTTGKC GSVTVRLIPA PRGSGIVASP AVKKLLQLAG V EDVYTQSN GKTRTLENTL KAAFVAIGNT YGFLTPNLWA EQPLPVSPLD IYSDEASAQ

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #20: 40S ribosomal protein S4-A

• Macromolecule: Name: 40S ribosomal protein S4-A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 29.338133 KDa

Sequence

String:

ARGPKKHLKR LAAPHHWLLD KLSGCYAPRP SAGPHKLRES LPLIVFLRNR LKYALNGREV KAILMQRHVK VDGKVRTDTT YPAGFMDVI TLDATNENFR LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP DPNIKVNDTV K IDLASGKI TDFIKFDAGK LVYVTGGRNL GRIGTIVHKE RHDGGFDLVH IKDSLDNTFV TRLNNVFVIG EQGKPYISLP KG KGIKLSI AEERDRRRAQ QGL

UniProtKB: Small ribosomal subunit protein eS4A

Macromolecule #21: 40S ribosomal protein S6-A

• Macromolecule: Name: 40S ribosomal protein S6-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 24.965975 KDa

Sequence

String:

MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE

UniProtKB: Small ribosomal subunit protein eS6A

Macromolecule #22: 40S ribosomal protein S7-A

• Macromolecule: Name: 40S ribosomal protein S7-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 21.07157 KDa

Sequence

String:

APQAKILSQA PTELELQVAQ AFVELENSSP ELKAELRPLQ FKSIREIDVA GGKKALAIFV PVPSLAGFHK VQTKLTRELE KKFQDRHVI FLAERRILPK PSRTSRQVQK RPRSRTLTAV HDKILEDLVF PTEIVGKRVR YLVGGNKIQK VLLDSKDVQQ I DYKLESFQ AVYNKLTGKQ IVFEIPS

UniProtKB: Small ribosomal subunit protein eS7A

Macromolecule #23: 40S ribosomal protein S8-A

• Macromolecule: Name: 40S ribosomal protein S8-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 21.14718 KDa

Sequence

String:

GISRDSRHKR SATGAKRAQF RKKRKFELGR QPANTKIGAK RIHSVRTRGG NKKYRALRIE TGNFSWASEG ISKKTRIAGV VYHPSNNEL VRTNTLTKAA IVQIDATPFR QWFEAHYGQT LGKKSKNAER KWAARAASAK IESSVESQFS AGRLYACISS R PGQSGRCD GYILEGEELA FYLRRLTAKK

UniProtKB: Small ribosomal subunit protein eS8A

Macromolecule #24: 40S ribosomal protein S9-A

• Macromolecule: Name: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 21.210662 KDa

Sequence

String:

PRAPRTYSKT YSTPKRPYES SRLDAELKLA GEFGLKNKKE IYRISFQLSK IRRAARDLLT RDEKDPKRLF EGNALIRRLV RVGVLSEDK KKLDYVLALK VEDFLERRLQ TQVYKLGLAK SVHHARVLIT QRHIAVGKQI VNIPSFMVRL DSEKHIDFAP T SPFGGARP GRVARRNAAR KAEASGE

UniProtKB: Small ribosomal subunit protein uS4A

Macromolecule #25: 40S ribosomal protein S11-A

• Macromolecule: Name: 40S ribosomal protein S11-A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 16.588461 KDa

Sequence

String:

STELTVQSER AFQKQPHIFN NPKVKTSKRT KRWYKNAGLG FKTPKTAIEG SYIDKKCPFT GLVSIRGKIL TGTVVSTKMH RTIVIRRAY LHYIPKYNRY EKRHKNVPVH VSPAFRVQVG DIVTVGQCRP ISKTVRFNVV KVSAAAG

UniProtKB: Small ribosomal subunit protein uS17A

Macromolecule #26: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 16.928748 KDa

Sequence

String:

GRMHSAGKGI SSSAIPYSRN APAWFKLSSE SVIEQIVKYA RKGLTPSQIG VLLRDAHGVT QARVITGNKI MRILKSNGLA PEIPEDLYY LIKKAVSVRK HLERNRKDKD AKFRLILIES RIHRLARYYR TVAVLPPNWK YESATASALV N

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #27: 40S ribosomal protein S14-B

• Macromolecule: Name: 40S ribosomal protein S14-B / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 13.560528 KDa

Sequence

String:

NSQVFGVARI YASFNDTFVH VTDLSGKETI ARVTGGMKVK ADRDESSPYA AMLAAQDVAA KCKEVGITAV HVKIRATGGT RTKTPGPGG QAALRALARS GLRIGRIEDV TPVPSDSTRK KGGRRGRRL

UniProtKB: Small ribosomal subunit protein uS11B

Macromolecule #28: 40S ribosomal protein S21-A

• Macromolecule: Name: 40S ribosomal protein S21-A / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 9.758829 KDa

Sequence

String:

MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR

UniProtKB: Small ribosomal subunit protein eS21A

Macromolecule #29: 40S ribosomal protein S22-A

• Macromolecule: Name: 40S ribosomal protein S22-A / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 14.518867 KDa

Sequence

String:

TRSSVLADAL NAINNAEKTG KRQVLIRPSS KVIIKFLQVM QKHGYIGEFE YIDDHRSGKI VVQLNGRLNK CGVISPRFNV KIGDIEKWT ANLLPARQFG YVILTTSAGI MDHEEARRKH VSGKILGFVY

UniProtKB: Small ribosomal subunit protein uS8A

Macromolecule #30: 40S ribosomal protein S23-A

• Macromolecule: Name: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 15.942699 KDa

Sequence

String:

GKGKPRGLNS ARKLRVHRRN NRWAENNYKK RLLGTAFKSS PFGGSSHAKG IVLEKLGIES KQPNSAIRKC VRVQLIKNGK KVTAFVPND GCLNFVDEND EVLLAGFGRK GKAKGDIPGV RFKVVKVSGV SLLALWKEKK EKPRS

UniProtKB: Small ribosomal subunit protein uS12A

Macromolecule #31: 40S ribosomal protein S24-A

• Macromolecule: Name: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 15.23165 KDa

Sequence

String:

SDAVTIRTRK VISNPLLARK QFVVDVLHPN RANVSKDELR EKLAEVYKAE KDAVSVFGFR TQFGGGKSVG FGLVYNSVAE AKKFEPTYR LVRYGLAEKV EKASRQQRKQ KKNRDKKIFG TGKRLAKKVA RRNAD

UniProtKB: Small ribosomal subunit protein eS24A

Macromolecule #32: 40S ribosomal protein S26-B

• Macromolecule: Name: 40S ribosomal protein S26-B / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 11.022989 KDa

Sequence

String:

PKKRASNGRN KKGRGHVKPV RCVNCSKSIP KDKAIKRMAI RNIVEAAAVR DLSEASVYPE YALPKTYNKL HYCVSCAIHA RIVRVRSRE DRKNRAPP

UniProtKB: Small ribosomal subunit protein eS26B

Macromolecule #33: 40S ribosomal protein S27-A

• Macromolecule: Name: 40S ribosomal protein S27-A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 8.762195 KDa

Sequence

String:

VLVQDLLHPT AASEARKHKL KTLVQGPRSY FLDVKCPGCL NITTVFSHAQ TAVTCESCST ILCTPTGGKA KLSEGTSFRR K

UniProtKB: Small ribosomal subunit protein eS27A

Macromolecule #34: 40S ribosomal protein S30-A

• Macromolecule: Name: 40S ribosomal protein S30-A / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 5.054081 KDa

Sequence

String:

AKVHGSLARA GKVKSQTPKV EKTEKPKKPK GRAYKRLLYT RRFV

UniProtKB: Small ribosomal subunit protein eS30A

Macromolecule #35: Multiprotein-bridging factor 1

• Macromolecule: Name: Multiprotein-bridging factor 1 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 13.247083 KDa

Sequence

String:

ARSQGQINAA RRQGLVVSVD KKYGSTNTRG DNEGQRLTKV DRETDIVKPK KLDPNVGRAI SRARTDKKMS QKDLATKINE KPTVVNDYE AARAIPNQQV LSKLERALGV KLRGNNIGSP L

UniProtKB: Multiprotein-bridging factor 1

Macromolecule #42: 60S ribosomal protein L2-A

• Macromolecule: Name: 60S ribosomal protein L2-A / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 27.089158 KDa

Sequence

String:

GRVIRNQRKG AGSIFTSHTR LRQGAAKLRT LDYAERHGYI RGIVKQIVHD SGRGAPLAKV VFRDPYKYRL REEIFIANEG VHTGQFIYA GKKASLNVGN VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL PSGAKKVISS D ARGVIGVI AGGGRVDKPL LKAGRAFHKY RLKRNSWPKT RGVAMNPVDH PHGGGNHQHI GKASTISRGA VSGQKAGLIA AR RTGLLRG SQKT

UniProtKB: Large ribosomal subunit protein uL2A

Macromolecule #43: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 43.719602 KDa

Sequence

String:

SHRKYEAPRH GHLGFLPRKR AASIRARVKA FPKDDRSKPV ALTSFLGYKA GMTTIVRDLD RPGSKFHKRE VVEAVTVVDT PPVVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK NWYKSKKKAF TKYSAKYAQD GAGIERELAR IKKYASVVRV L VHTQIRKT PLAQKKAHLA EIQLNGGSIS EKVDWAREHF EKTVAVDSVF EQNEMIDAIA VTKGHGFEGV THRWGTKKLP RK THRGLRK VACIGAWHPA HVMWSVARAG QRGYHSRTSI NHKIYRVGKG DDEANGATSF DRTKKTITPM GGFVHYGEIK NDF IMVKGC IPGNRKRIVT LRKSLYTNTS RKALEEVSLK WIDTASKFGK GRFQTPAEKH AFMGTLKKDL

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #44: 60S ribosomal protein L4-A

• Macromolecule: Name: 60S ribosomal protein L4-A / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 39.027926 KDa

Sequence

String:

SRPQVTVHSL TGEATANALP LPAVFSAPIR PDIVHTVFTS VNKNKRQAYA VSEKAGHQTS AESWGTGRAV ARIPRVGGGG TGRSGQGAF GNMCRGGRMF APTKTWRKWN VKVNHNEKRY ATASAIAATA VASLVLARGH RVEKIPEIPL VVSTDLESIQ K TKEAVAAL KAVGAHSDLL KVLKSKKLRA GKGKYRNRRW TQRRGPLVVY AEDNGIVKAL RNVPGVETAN VASLNLLQLA PG AHLGRFV IWTEAAFTKL DQVWGSETVA SSKVGYTLPS HIISTSDVTR IINSSEIQSA IRPAGQATQK RTHVLKKNPL KNK QVLLRL NPYAKVFAAE KLGSKKAEKT GTKPAAVFTE TLKHD

UniProtKB: Large ribosomal subunit protein uL4A

Macromolecule #45: 60S ribosomal protein L5

• Macromolecule: Name: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 33.415387 KDa

Sequence

String:

QKDAKSSAYS SRFQTPFRRR REGKTDYYQR KRLVTQHKAK YNTPKYRLVV RFTNKDIICQ IISSTITGDV VLAAAYSHEL PRYGITHGL TNWAAAYATG LLIARRTLQK LGLDETYKGV EEVEGEYELT EAVEDGPRPF KVFLDIGLQR TTTGARVFGA L KGASDGGL YVPHSENRFP GWDFETEEID PELLRSYIFG GHVSQYMEEL ADDDEERFSE LFKGYLADDI DADSLEDIYT SA HEAIRAD PAFKPTEKKF TKEQYAAESK KYRQTKLSKE ERAARVAAKI AALAGQQ

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #46: 60S ribosomal protein L6-B

• Macromolecule: Name: 60S ribosomal protein L6-B / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 18.93532 KDa

Sequence

String:

TAQQAPKWYP SEDVAAPKKT RKAVRPQKLR ASLVPGTVLI LLAGRFRGKR VVYLKHLEDN TLLVTGPFKV NGVPLRRVNA RYVIATSTK VSVEGVNVEK FNVEYFAKEK LTKKEKKEAK EIKTERVEDQ KVVDKALLAE IKKTPLLKQY LSASFSLKNG D KPHLLKF

UniProtKB: Large ribosomal subunit protein eL6B

Macromolecule #47: 60S ribosomal protein L7-A

• Macromolecule: Name: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 25.240377 KDa

Sequence

String:

AEQVAAERAA RKAANKEKRA IILERNAAYQ KEYETAERNI IQAKRDAKAA GSYYVEAQHK LVFVVRIKGI NKIPPKPRKV LQLLRLTRI NSGTFVKVTK ATLELLKLIE PYVAYGYPSY STIRQLVYKR GFGKINKQRV PLSDNAIIEA NLGKYGILSI D DLIHEIIT VGPHFKQANN FLWPFKLSNP SGGWGVPRKF KHFIQGGSFG NREEFINKLV KSMN

UniProtKB: Large ribosomal subunit protein uL30A

Macromolecule #48: 60S ribosomal protein L8-A

• Macromolecule: Name: 60S ribosomal protein L8-A / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 25.814998 KDa

Sequence

String:

NPLTHSTPKN FGIGQAVQPK RNLSRYVKWP EYVRVQRQKK ILSIRLKVPP TIAQFQYTLD RNTAAETFKL FNKYRPETAA EKKERLTKE AAAVAEGKSK QDASPKPYAV KYGLNHVVAL IENKKAKLVL IANDVDPIEL VVFLPALCKK MGVPYAIVKG K ARLGTLVN QKTSAVAALT EVRAEDEAAL AKLVSTIDAN FADKYDEVKK HWGGGILGNK AQAKMDKRAK NSDSA

UniProtKB: Large ribosomal subunit protein eL8A

Macromolecule #49: 60S ribosomal protein L9-A

• Macromolecule: Name: 60S ribosomal protein L9-A / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 21.605061 KDa

Sequence

String:

MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

UniProtKB: Large ribosomal subunit protein uL6A

Macromolecule #50: 60S ribosomal protein L10

• Macromolecule: Name: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 25.080062 KDa

Sequence

String:

ARRPARCYRY QKNKPYPKSR YNRAVPDSKI RIYDLGKKKA TVDEFPLCVH LVSNELEQLS SEALEAARIC ANKYMTTVSG RDAFHLRVR VHPFHVLRIN KMLSCAGADR LQQGMRGAWG KPHGLAARVD IGQIIFSVRT KDSNKDVVVE GLRRARYKFP G QQKIILSK KWGFTNLDRP EYLKKREAGE VKDDGAFVKF LSKKGSLENN IREFPEYFAA

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #51: 60S ribosomal protein L11-B

• Macromolecule: Name: 60S ribosomal protein L11-B / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 19.266082 KDa

Sequence

String:

QNPMRDLKIE KLVLNISVGE SGDRLTRASK VLEQLSGQTP VQSKARYTVR TFGIRRNEKI AVHVTVRGPK AEEILERGLK VKEYQLRDR NFSATGNFGF GIDEHIDLGI KYDPSIGIFG MDFYVVMNRP GARVTRRKRC KGTVGNSHKT TKEDTVSWFK Q KYDADVLD K

UniProtKB: Large ribosomal subunit protein uL5B

Macromolecule #52: 60S ribosomal protein L13-A

• Macromolecule: Name: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 21.885234 KDa

Sequence

String:

AISKNLPILK NHFRKHWQER VKVHFDQAGK KVSRRNARAT RAAKIAPRPL DLLRPVVRAP TVKYNRKVRA GRGFTLAEVK AAGLTAAYA RTIGIAVDHR RQNRNQEIFD ANVQRLKEYQ SKIIVFPRNG KAPEAEQVLS AAATFPIAQP ATDVEARAVQ D NGESAFRT LRLARSEKKF RGIREKRARE KAEAE

UniProtKB: LPS-assembly protein LptD

Macromolecule #53: 60S ribosomal protein L14-A

• Macromolecule: Name: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 14.976794 KDa

Sequence

String:

TDSIVKASNW RLVEVGRVVL IKKGQSAGKL AAIVEIIDQK KVLIDGPKAG VPRQAINLGQ VVLTPLTFAL PRGARTATVS KKWAAAAVC EKWAASSWAK KIAQRERRAA LTDFERFQVM VLRKQKRYTV KKALAKA

UniProtKB: Large ribosomal subunit protein eL14A

Macromolecule #54: 60S ribosomal protein L15-A

• Macromolecule: Name: 60S ribosomal protein L15-A / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 24.351162 KDa

Sequence

String:

GAYKYLEELQ RKKQSDVLRF LQRVRVWEYR QKNVIHRAAR PTRPDKARRL GYKAKQGFVI YRVRVRRGNR KRPVPKGATY GKPTNQGVN ELKYQRSLRA TAEERVGRRA ANLRVLNSYW VNQDSTYKYF EVILVDPQHK AIRRDARYNW ICDPVHKHRE A RGLTATGK KSRGINKGHK FNNTKAGRRK TWKRQNTLSL WRYRK

UniProtKB: Large ribosomal subunit protein eL15A

Macromolecule #55: 60S ribosomal protein L16-A

• Macromolecule: Name: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 22.028951 KDa

Sequence

String:

VEPVVVIDGK GHLVGRLASV VAKQLLNGQK IVVVRAEELN ISGEFFRNKL KYHDFLRKAT AFNKTRGPFH FRAPSRIFYK ALRGMVSHK TARGKAALER LKVFEGIPPP YDKKKRVVVP QALRVLRLKP GRKYTTLGKL STSVGWKYED VVAKLEAKRK V SSAEYYAK KRAFTKKVAS ANATAAESDV AKQLAALGY

UniProtKB: Large ribosomal subunit protein uL13A

Macromolecule #56: 60S ribosomal protein L17-A

• Macromolecule: Name: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 20.458322 KDa

Sequence

String:

ARYGATSTNP AKSASARGSY LRVSFKNTRE TAQAINGWEL TKAQKYLEQV LDHQRAIPFR RFNSSIGRTA QGKEFGVTKA RWPAKSVKF VQGLLQNAAA NAEAKGLDAT KLYVSHIQVN QAPKQRRRTY RAHGRINKYE SSPSHIELVV TEKEEAVAKA A EKKVVRLT SRQRGRIAAQ KRIAA

UniProtKB: Large ribosomal subunit protein uL22A

Macromolecule #57: 60S ribosomal protein L18-A

• Macromolecule: Name: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 20.478059 KDa

Sequence

String:

GIDHTSKQHK RSGHRTAPKS DNVYLKLLVK LYTFLARRTD APFNKVVLKA LFLSKINRPP VSVSRIARAL KQEGAANKTV VVVGTVTDD ARIFEFPKTT VAALRFTAGA RAKIVKAGGE CITLDQLAVR APKGQNTLIL RGPRNSREAV RHFGMGPHKG K APRILSTG RKFERARGRR RSKGFKV

UniProtKB: Large ribosomal subunit protein eL18A

Macromolecule #58: 60S ribosomal protein L19-A

• Macromolecule: Name: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 21.631121 KDa

Sequence

String:

ANLRTQKRLA ASVVGVGKRK VWLDPNETSE IAQANSRNAI RKLVKNGTIV KKAVTVHSKS RTRAHAQSKR EGRHSGYGKR KGTREARLP SQVVWIRRLR VLRRLLAKYR DAGKIDKHLY HVLYKESKGN AFKHKRALVE HIIQAKADAQ REKALNEEAE A RRLKNRAA RDRRAQRVAE KRDALLKEDA

UniProtKB: Large ribosomal subunit protein eL19A

Macromolecule #59: 60S ribosomal protein L20-A

• Macromolecule: Name: 60S ribosomal protein L20-A / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 20.347654 KDa

Sequence

String:

AHFKEYQVIG RRLPTESVPE PKLFRMRIFA SNEVIAKSRY WYFLQKLHKV KKASGEIVSI NQINEAHPTK VKNFGVWVRY DSRSGTHNM YKEIRDVSRV AAVETLYQDM AARHRARFRS IHILKVAEIE KTADVKRQYV KQFLTKDLKF PLPHRVQKST K TFSYKRPS TFY

UniProtKB: Large ribosomal subunit protein eL20A

Macromolecule #60: 60S ribosomal protein L21-A

• Macromolecule: Name: 60S ribosomal protein L21-A / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 18.148066 KDa

Sequence

String:

GKSHGYRSRT RYMFQRDFRK HGAVHLSTYL KVYKVGDIVD IKANGSIQKG MPHKFYQGKT GVVYNVTKSS VGVIINKMVG NRYLEKRLN LRVEHIKHSK CRQEFLERVK ANAAKRAEAK AQGVAVQLKR QPAQPRESRI VSTEGNVPQT LAPVPYETFI

UniProtKB: Large ribosomal subunit protein eL21A

Macromolecule #61: 60S ribosomal protein L22-A

• Macromolecule: Name: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 11.263863 KDa

Sequence

String:

QKIAKTFTVD VSSPTENGVF DPASYAKYLI DHIKVEGAVG NLGNAVTVTE DGTVVTVVST AKFSGKYLKY LTKKYLKKNQ LRDWIRFVS TKTNEYRLAF Y

UniProtKB: Large ribosomal subunit protein eL22A

Macromolecule #62: 60S ribosomal protein L23-A

• Macromolecule: Name: 60S ribosomal protein L23-A / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 14.362755 KDa

Sequence

String:

SGNGAQGTKF RISLGLPVGA IMNCADNSGA RNLYIIAVKG SGSRLNRLPA ASLGDMVMAT VKKGKPELRK KVMPAIVVRQ AKSWRRRDG VFLYFEDNAG VIANPKGEMK GSAITGPVGK ECADLWPRVA SNSGVVV

UniProtKB: Large ribosomal subunit protein uL14A

Macromolecule #63: 60S ribosomal protein L24-A

• Macromolecule: Name: 60S ribosomal protein L24-A / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 7.214498 KDa

Sequence

String:

MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK K

UniProtKB: Large ribosomal subunit protein eL24A

Macromolecule #64: 60S ribosomal protein L25

• Macromolecule: Name: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 13.771176 KDa

Sequence

String:

KALKVRTSAT FRLPKTLKLA RAPKYASKAV PHYNRLDSYK VIEQPITSET AMKKVEDGNI LVFQVSMKAN KYQIKKAVKE LYEVDVLKV NTLVRPNGTK KAYVRLTADY DALDIANRIG YI

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #65: 60S ribosomal protein L26-A

• Macromolecule: Name: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 14.005475 KDa

Sequence

String:

AKQSLDVSSD RRKARKAYFT APSSQRRVLL SAPLSKELRA QYGIKALPIR RDDEVLVVRG SKKGQEGKIS SVYRLKFAVQ VDKVTKEKV NGASVPINLH PSKLVITKLH LDKDRKALIQ RKGGKL

UniProtKB: Large ribosomal subunit protein uL24A

Macromolecule #66: 60S ribosomal protein L27-A

• Macromolecule: Name: 60S ribosomal protein L27-A / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 15.437166 KDa

Sequence

String:

AKFLKAGKVA VVVRGRYAGK KVVIVKPHDE GSKSHPFGHA LVAGIERYPL KVTKKHGAKK VAKRTKIKPF IKVVNYNHLL PTRYTLDVE AFKSVVSTET FEQPSQREEA KKVVKKAFEE RHQAGKNQWF FSKLRF

UniProtKB: Large ribosomal subunit protein eL27A

Macromolecule #67: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 16.630471 KDa

Sequence

String:

PSRFTKTRKH RGHVSAGKGR IGKHRKHPGG RGMAGGQHHH RINMDKYHPG YFGKVGMRYF HKQQAHFWKP VLNLDKLWTL IPEDKRDQY LKSASKETAP VIDTLAAGYG KILGKGRIPN VPVIVKARFV SKLAEEKIRA AGGVVELIA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #68: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 6.560688 KDa

Sequence

String:

AKSKNHTAHN QTRKAHRNGI KKPKTYKYPS LKGVDPKFRR NHKHALHGTA KALAAAKK

UniProtKB: Large ribosomal subunit protein eL29

Macromolecule #69: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 10.487266 KDa

Sequence

String:

SINQKLALVI KSGKYTLGYK STVKSLRQGK SKLIIIAANT PVLRKSELEY YAMLSKTKVY YFQGGNNELG TAVGKLFRVG VVSILEAGD SDILTTL

UniProtKB: Putative major capsid protein p72

Macromolecule #70: 60S ribosomal protein L31-A

• Macromolecule: Name: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 12.649754 KDa

Sequence

String:

LKDVVTREYT INLHKRLHGV SFKKRAPRAV KEIKKFAKLH MGTDDVRLAP ELNQAIWKRG VKGVEYRLRL RISRKRNEEE DAKNPLFSY VEPVLVASAK GLQTVVVEED

UniProtKB: Large ribosomal subunit protein eL31A

Macromolecule #71: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 14.478054 KDa

Sequence

String:

ASLPHPKIVK KHTKKFKRHH SDRYHRVAEN WRKQKGIDSV VRRRFRGNIS QPKIGYGSNK KTKFLSPSGH KTFLVANVKD LETLTMHTK TYAAEIAHNI SAKNRVVILA RAKALGIKVT NPKGRLAL

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #72: 60S ribosomal protein L33-A

• Macromolecule: Name: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 12.045935 KDa

Sequence

String:

AESHRLYVKG KHLSYQRSKR VNNPNVSLIK IEGVATPQDA QFYLGKRIAY VYRASKEVRG SKIRVMWGKV TRTHGNSGVV RATFRNNLP AKTFGASVRI FLYPSNI

UniProtKB: Large ribosomal subunit protein eL33A

Macromolecule #73: 60S ribosomal protein L34-A

• Macromolecule: Name: 60S ribosomal protein L34-A / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 12.608831 KDa

Sequence

String:

AQRVTFRRRN PYNTRSNKIK VVKTPGGILR AQHVKKLATR PKCGDCGSAL QGISTLRPRQ YATVSKTHKT VSRAYGGSRC ANCVKERII RAFLIEEQKI VKKVVKEQTE AAK

UniProtKB: Large ribosomal subunit protein eL34A

Macromolecule #74: 60S ribosomal protein L35-A

• Macromolecule: Name: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 13.811444 KDa

Sequence

String:

AGVKAYELRT KSKEQLASQL VDLKKELAEL KVQKLSRPSL PKIKTVRKSI ACVLTVINEQ QREAVRQLYK GKKYQPKDLR AKKTRALRR ALTKFEASQV TEKQRKKQIA FPQRKYAIKA

UniProtKB: Large ribosomal subunit protein uL29A

Macromolecule #75: 60S ribosomal protein L36-A

• Macromolecule: Name: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 11.020063 KDa

Sequence

String:

TVKTGIAIGL NKGKKVTSMT PAPKISYKKG AASNRTKFVR SLVREIAGLS PYERRLIDLI RNSGEKRARK VAKKRLGSFT RAKAKVEEM NNIIAASRRH

UniProtKB: Large ribosomal subunit protein eL36A

Macromolecule #76: 60S ribosomal protein L37-A

• Macromolecule: Name: 60S ribosomal protein L37-A / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 9.229632 KDa

Sequence

String:

GKGTPSFGKR HNKSHTLCNR CGRRSFHVQK KTCSSCGYPA AKTRSYNWGA KAKRRHTTGT GRMRYLKHVS RRFKNGFQTG S

UniProtKB: Large ribosomal subunit protein eL37A

Macromolecule #77: 60S ribosomal protein L38

• Macromolecule: Name: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 8.714363 KDa

Sequence

String:

AREITDIKQF LELTRRADVK TATVKINKKL NKAGKPFRQT KFKVRGSSSL YTLVINDAGK AKKLIQSLPP TLKVNRL

UniProtKB: Large ribosomal subunit protein eL38

Macromolecule #78: 60S ribosomal protein L39

• Macromolecule: Name: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 6.227444 KDa

Sequence

String:

AAQKSFRIKQ KMAKAKKQNR PLPQWIRLRT NNTIRYNAKR RNWRRTKMNI

UniProtKB: Large ribosomal subunit protein eL39

Macromolecule #79: Ubiquitin-60S ribosomal protein L40

• Macromolecule: Name: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 6.032321 KDa

Sequence

String:

IIEPSLKALA SKYNCDKSVC RKCYARLPPR ATNCRKRKCG HTNQLRPKKK LK

UniProtKB: Ubiquitin-ribosomal protein eL40A fusion protein

Macromolecule #80: 60S ribosomal protein L41-B

• Macromolecule: Name: 60S ribosomal protein L41-B / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 3.354243 KDa

Sequence

String:

MRAKWRKKRT RRLKRKRRKV RARSK

UniProtKB: Small ribosomal subunit protein eS32B

Macromolecule #81: 60S ribosomal protein L42-A

• Macromolecule: Name: 60S ribosomal protein L42-A / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 11.840159 KDa

Sequence

String:

VNVPKTRKTY CKGKTCRKHT QHKVTQYKAG KASLFAQGKR RYDRKQSGFG GQTKPVFHKK AKTTKKVVLR LECVKCKTRA QLTLKRCKH FELGGEKKQK GQAL

UniProtKB: Large ribosomal subunit protein eL42A

Macromolecule #82: 60S ribosomal protein L43-A

• Macromolecule: Name: 60S ribosomal protein L43-A / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288c (yeast)
• Molecular weight: Theoretical: 9.981756 KDa

Sequence

String:

AKRTKKVGIT GKYGVRYGSS LRRQVKKLEI QQHARYDCSF CGKKTVKRGA AGIWTCSCCK KTVAGGAYTV STAAAATVRS TIRRLREMV EA

UniProtKB: Large ribosomal subunit protein eL43A

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Grid: Model: Quantifoil R3/3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 300
• Vitrification: Cryogen name: ETHANE-PROPANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 2.5 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: PDB ENTRY
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.36 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 30016
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD

Atomic model buiding 1

• Refinement: Space: REAL / Protocol: RIGID BODY FIT

Output model

PDB-7nrd:
Structure of the yeast Gcn1 bound to a colliding stalled 80S ribosome with MBF1, A/P-tRNA and P/E-tRNA