EMDB-12534: Structure of the yeast Gcn1 bound to a leading stalled 80S riboso...

Basic information

• Entry: Database: EMDB / ID: EMD-12534
• Title: Structure of the yeast Gcn1 bound to a leading stalled 80S ribosome with Rbg2, Gir2, A- and P-tRNA and eIF5A

Sample

• Complex: Structure of the yeast Gcn1-bound leading stalled 80S ribosome with bound Rbg2, Gir2, A- and P-tRNA and eIF5A
  • RNA: x 7 types
  • Protein or peptide: x 79 types

Function / homology

Function:

positive regulation of cytoplasmic translational elongation through polyproline stretches / Hypusine synthesis from eIF5A-lysine / CAT tailing / translational frameshifting / positive regulation of translational termination / positive regulation of translational elongation / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / positive regulation of cellular response to amino acid starvation / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / Protein methylation / RMTs methylate histone arginines / positive regulation of translational fidelity / mTORC1-mediated signalling / ribosome-associated ubiquitin-dependent protein catabolic process / Protein hydroxylation / GDP-dissociation inhibitor activity / : / pre-mRNA 5'-splice site binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Ribosomal scanning and start codon recognition / preribosome, small subunit precursor / translational elongation / response to cycloheximide / mRNA destabilization / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / 90S preribosome / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / protein-RNA complex assembly / ribosomal small subunit export from nucleus / preribosome, large subunit precursor / positive regulation of translational initiation / L13a-mediated translational silencing of Ceruloplasmin expression / translation regulator activity / ribosomal large subunit export from nucleus / G-protein alpha-subunit binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / positive regulation of protein kinase activity / translation elongation factor activity / rescue of stalled ribosome / translational termination / maturation of SSU-rRNA / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / DNA-(apurinic or apyrimidinic site) endonuclease activity / cellular response to amino acid starvation / ribosome assembly / translation initiation factor activity / small-subunit processome / protein kinase C binding / maintenance of translational fidelity / macroautophagy / modification-dependent protein catabolic process / ribosomal small subunit biogenesis / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / protein tag activity / ribosomal small subunit assembly / rRNA processing / cytoplasmic stress granule / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / ribosome binding / ribosome biogenesis / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / positive regulation of protein phosphorylation / translation / G protein-coupled receptor signaling pathway / negative regulation of gene expression / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / GTP binding / nucleolus / perinuclear region of cytoplasm / mitochondrion / RNA binding / zinc ion binding / nucleoplasm / metal ion binding

Domain/homology:

GTP binding protein, second domain / C-terminal region of MMR_HSR1 domain / GTP1/OBG, conserved site / GTP1/OBG family signature. / : / Translation initiation factor 5A-like, N-terminal / Translation elongation factor, IF5A, hypusine site / Eukaryotic initiation factor 5A hypusine signature. / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / Translation elongation factor IF5A-like / Translation elongation factor, IF5A C-terminal / Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold / TGS domain / TGS domain profile. / TGS / TGS-like / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / 50S ribosome-binding GTPase / GTP binding domain / : / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S12e / : / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S21e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S19e, conserved site / S27a-like superfamily / Ribosomal protein S26e / Ribosomal protein L29e / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S25 / Ribosomal protein S26e signature. / Ribosomal protein S17e, conserved site / : / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal L29e protein family / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S8e subdomain, eukaryotes / S25 ribosomal protein / Ribosomal protein L10e, conserved site / Ribosomal protein L10e / Ribosomal protein L27e, conserved site / Ribosomal protein S19A/S15e / Ribosomal protein S21e signature. / Ribosomal protein S30 / Ribosomal protein S3Ae, conserved site / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S11, N-terminal / : / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein L24e, conserved site / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein L34e, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal S17 / Ribosomal protein S19e signature. / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / Ribosomal protein L23/L25, N-terminal / 40S Ribosomal protein S10 / 60S ribosomal protein L35 / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S28e / Ribosomal protein L35Ae, conserved site / Ribosomal protein L30e, conserved site / 40S ribosomal protein S4 C-terminus

Component:

Small ribosomal subunit protein uS4A / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL24A / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein eL6B / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL22A / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS15 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein eS21A / Small ribosomal subunit protein uS8A / Large ribosomal subunit protein eL27A / Large ribosomal subunit protein eL31A / Ubiquitin-ribosomal protein eL40A fusion protein / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL43A / Large ribosomal subunit protein eL42A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS30A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Large ribosomal subunit protein uL14A / Large ribosomal subunit protein uL2A / Small ribosomal subunit protein uS17A / Large ribosomal subunit protein eL18A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / Large ribosomal subunit protein eL19A / Large ribosomal subunit protein uL29A / Small ribosomal subunit protein eS32A / Large ribosomal subunit protein uL4A / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein eS17B / Large ribosomal subunit protein eL8A / Eukaryotic translation initiation factor 5A-1 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL13A / Small ribosomal subunit protein eS7A / Small ribosomal subunit protein uS2A / Small ribosomal subunit protein eS1A / Small ribosomal subunit protein eS27A / Large ribosomal subunit protein eL14A / Small ribosomal subunit protein RACK1 / Large ribosomal subunit protein eL32 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS11B / Small ribosomal subunit protein eS26B / Small ribosomal subunit protein uS14A / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL37A / Large ribosomal subunit protein eL38 / Ribosome-interacting GTPase 2 / Large ribosomal subunit protein eL34A / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL21A / Small ribosomal subunit protein eS10A / Large ribosomal subunit protein eL13A / Large ribosomal subunit protein uL5B / Small ribosomal subunit protein eS25A / Small ribosomal subunit protein eS28A

• Biological species: Saccharomyces cerevisiae S288C (yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 3.9 Å
• Authors: Pochopien AA / Beckert B / Wilson DN

Funding support

Germany, 2 items

Organization	Grant number	Country
German Research Foundation (DFG)	WI3285/8-1	Germany
German Research Foundation (DFG)	SPP-1879	Germany

• Citation: Journal: Proc Natl Acad Sci U S A / Year: 2021; Title: Structure of Gcn1 bound to stalled and colliding 80S ribosomes.; Authors: Agnieszka A Pochopien / Bertrand Beckert / Sergo Kasvandik / Otto Berninghausen / Roland Beckmann / Tanel Tenson / Daniel N Wilson / ; Abstract: The Gcn pathway is conserved in all eukaryotes, including mammals such as humans, where it is a crucial part of the integrated stress response (ISR). Gcn1 serves as an essential effector protein for the kinase Gcn2, which in turn is activated by stalled ribosomes, leading to phosphorylation of eIF2 and a subsequent global repression of translation. The fine-tuning of this adaptive response is performed by the Rbg2/Gir2 complex, a negative regulator of Gcn2. Despite the wealth of available biochemical data, information on structures of Gcn proteins on the ribosome has remained elusive. Here we present a cryo-electron microscopy structure of the yeast Gcn1 protein in complex with stalled and colliding 80S ribosomes. Gcn1 interacts with both 80S ribosomes within the disome, such that the Gcn1 HEAT repeats span from the P-stalk region on the colliding ribosome to the P-stalk and the A-site region of the lead ribosome. The lead ribosome is stalled in a nonrotated state with peptidyl-tRNA in the A-site, uncharged tRNA in the P-site, eIF5A in the E-site, and Rbg2/Gir2 in the A-site factor binding region. By contrast, the colliding ribosome adopts a rotated state with peptidyl-tRNA in a hybrid A/P-site, uncharged-tRNA in the P/E-site, and Mbf1 bound adjacent to the mRNA entry channel on the 40S subunit. Collectively, our findings reveal the interaction mode of the Gcn2-activating protein Gcn1 with colliding ribosomes and provide insight into the regulation of Gcn2 activation. The binding of Gcn1 to a disome has important implications not only for the Gcn2-activated ISR, but also for the general ribosome-associated quality control pathways.

History

Deposition	Mar 3, 2021	-
Header (metadata) release	May 5, 2021	-
Map release	May 5, 2021	-
Update	May 5, 2021	-
Current status	May 5, 2021	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_12534.map.gz / Format: CCP4 / Size: 1.3 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.084 Å

Density

Contour Level	By AUTHOR: 0.03 / Movie #1: 0.03
Minimum - Maximum	-0.38923305 - 0.5115081
Average (Standard dev.)	0.0015342836 (±0.007732451)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 700 700 700 Spacing 700 700 700
Cell	A=B=C: 758.8 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.084	1.084	1.084
M x/y/z	700	700	700
origin x/y/z	0.000	0.000	0.000
length x/y/z	758.800	758.800	758.800
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	700	700	700
D min/max/mean	-0.389	0.512	0.002

Supplemental data

Additional map: #1

• File: emd_12534_additional_1.map

Additional map: #2

• File: emd_12534_additional_2.map

Additional map: #3

• File: emd_12534_additional_3.map

Additional map: #4

• File: emd_12534_additional_4.map

Additional map: #5

• File: emd_12534_additional_5.map

Additional map: #6

• File: emd_12534_additional_6.map

Additional map: #7

• File: emd_12534_additional_7.map

Additional map: #8

• File: emd_12534_additional_8.map

Half map: #1

• File: emd_12534_half_map_1.map

Half map: #2

• File: emd_12534_half_map_2.map

Sample components

Entire : Structure of the yeast Gcn1-bound leading stalled 80S ribosome wi...

• Entire: Name: Structure of the yeast Gcn1-bound leading stalled 80S ribosome with bound Rbg2, Gir2, A- and P-tRNA and eIF5A

Components

• Complex: Structure of the yeast Gcn1-bound leading stalled 80S ribosome with bound Rbg2, Gir2, A- and P-tRNA and eIF5A
  • RNA: 18S rRNA (1771-MER)
  • RNA: RNA (5'-R(P*AP*UP*GP*AP*AP*A)-3')
  • Protein or peptide: 40S ribosomal protein S0-ARibosome
  • Protein or peptide: 40S ribosomal protein S1-ARibosome
  • Protein or peptide: 40S ribosomal protein S15
  • Protein or peptide: 40S ribosomal protein S2
  • Protein or peptide: 40S ribosomal protein S3
  • Protein or peptide: 40S ribosomal protein S4-ARibosome
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S6-ARibosome
  • Protein or peptide: 40S ribosomal protein S7-ARibosome
  • Protein or peptide: 40S ribosomal protein S8-ARibosome
  • Protein or peptide: 40S ribosomal protein S9-ARibosome
  • Protein or peptide: 40S ribosomal protein S10-ARibosome
  • Protein or peptide: 40S ribosomal protein S11-ARibosome
  • Protein or peptide: 40S ribosomal protein S12
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: 40S ribosomal protein S14-BRibosome
  • Protein or peptide: 40S ribosomal protein S16-ARibosome
  • Protein or peptide: 40S ribosomal protein S17-BRibosome
  • Protein or peptide: 40S ribosomal protein S18-ARibosome
  • Protein or peptide: 40S ribosomal protein S19-ARibosome
  • Protein or peptide: 40S ribosomal protein S20
  • Protein or peptide: 40S ribosomal protein S21-ARibosome
  • Protein or peptide: 40S ribosomal protein S22-ARibosome
  • Protein or peptide: 40S ribosomal protein S23-ARibosome
  • Protein or peptide: 40S ribosomal protein S24-ARibosome
  • Protein or peptide: 40S ribosomal protein S25-ARibosome
  • Protein or peptide: 40S ribosomal protein S26-BRibosome
  • Protein or peptide: 40S ribosomal protein S27-ARibosome
  • Protein or peptide: 40S ribosomal protein S29-ARibosome
  • Protein or peptide: 40S ribosomal protein S30-ARibosome
  • Protein or peptide: 40S ribosomal protein S31
  • Protein or peptide: Guanine nucleotide-binding protein subunit beta-like protein
  • Protein or peptide: 40S ribosomal protein S28-ARibosome
  • RNA: tRNA (76-MER)
  • RNA: tRNA (75-MER)
  • Protein or peptide: Ribosome-interacting GTPase 2
  • Protein or peptide: GIR2
  • RNA: 25S rRNA (3184-MER)
  • RNA: 5S rRNA (121-MER)
  • RNA: 5.8S rRNA (158-MER)
  • Protein or peptide: 60S ribosomal protein L2-ARibosome
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: 60S ribosomal protein L4-ARibosome
  • Protein or peptide: 60S ribosomal protein L5
  • Protein or peptide: 60S ribosomal protein L6-BRibosome
  • Protein or peptide: 60S ribosomal protein L7-ARibosome
  • Protein or peptide: 60S ribosomal protein L8-ARibosome
  • Protein or peptide: 60S ribosomal protein L9-ARibosome
  • Protein or peptide: 60S ribosomal protein L10
  • Protein or peptide: 60S ribosomal protein L11-BRibosome
  • Protein or peptide: 60S ribosomal protein L13-ARibosome
  • Protein or peptide: 60S ribosomal protein L14-ARibosome
  • Protein or peptide: 60S ribosomal protein L15-ARibosome
  • Protein or peptide: 60S ribosomal protein L16-ARibosome
  • Protein or peptide: 60S ribosomal protein L17-ARibosome
  • Protein or peptide: 60S ribosomal protein L18-ARibosome
  • Protein or peptide: 60S ribosomal protein L19-ARibosome
  • Protein or peptide: 60S ribosomal protein L20-ARibosome
  • Protein or peptide: 60S ribosomal protein L21-ARibosome
  • Protein or peptide: 60S ribosomal protein L22-ARibosome
  • Protein or peptide: 60S ribosomal protein L23-ARibosome
  • Protein or peptide: 60S ribosomal protein L24-ARibosome
  • Protein or peptide: 60S ribosomal protein L25
  • Protein or peptide: 60S ribosomal protein L26-ARibosome
  • Protein or peptide: 60S ribosomal protein L27-ARibosome
  • Protein or peptide: 60S ribosomal protein L28
  • Protein or peptide: 60S ribosomal protein L29
  • Protein or peptide: 60S ribosomal protein L30
  • Protein or peptide: 60S ribosomal protein L31-ARibosome
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: 60S ribosomal protein L33-ARibosome
  • Protein or peptide: 60S ribosomal protein L34-ARibosome
  • Protein or peptide: 60S ribosomal protein L35-ARibosome
  • Protein or peptide: 60S ribosomal protein L36-ARibosome
  • Protein or peptide: 60S ribosomal protein L37-ARibosome
  • Protein or peptide: 60S ribosomal protein L38
  • Protein or peptide: 60S ribosomal protein L39
  • Protein or peptide: 60S ribosomal protein L40-ARibosome
  • Protein or peptide: 60S ribosomal protein L41-ARibosome
  • Protein or peptide: 60S ribosomal protein L42-ARibosome
  • Protein or peptide: 60S ribosomal protein L43-ARibosome
  • Protein or peptide: eiF5A
  • Protein or peptide: L1 60S ribosomal protein
  • Protein or peptide: GCN1

Supramolecule #1: Structure of the yeast Gcn1-bound leading stalled 80S ribosome wi...

• Supramolecule: Name: Structure of the yeast Gcn1-bound leading stalled 80S ribosome with bound Rbg2, Gir2, A- and P-tRNA and eIF5A; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#86
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)

Macromolecule #1: 18S rRNA (1771-MER)

• Macromolecule: Name: 18S rRNA (1771-MER) / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 579.761938 KDa

Sequence

String:

UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA AUACAUGCUU AAAAUCUCGA CCCUUUGGAA GAGAUGUAUU UAUUAGAUAA AAAAUCAAUG UCUUCGGACU CU UUGAUGA UUCAUAAUAA CUUUUCGAAU CGCAUGGCCU UGUGCUGGCG AUGGUUCAUU CAAAUUUCUG CCCUAUCAAC UUU CGAUGG UAGGAUAGUG GCCUACCAUG GUUUCAACGG GUAACGGGGA AUAAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAA ACGGC UACCACAUCC AAGGAAGGCA GCAGGCGCGC AAAUUACCCA AUCCUAAUUC AGGGAGGUAG UGACAAUAAA UAACG AUAC AGGGCCCAUU CGGGUCUUGU AAUUGGAAUG AGUACAAUGU AAAUACCUUA ACGAGGAACA AUUGGAGGGC AAGUCU GGU GCCAGCAGCC GCGGUAAUUC CAGCUCCAAU AGCGUAUAUU AAAGUUGUUG CAGUUAAAAA GCUCGUAGUU GAACUUU GG GCCCGGUUGG CCGGUCCGAU UUUUUCGUGU ACUGGAUUUC CAACGGGGCC UUUCCUUCUG GCUAACCUUG AGUCCUUG U GGCUCUUGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUGUUCAAA GCAGGCGUAU UGCUCGAAUA UAUUAGCAU GGAAUAAUAG AAUAGGACGU UUGGUUCUAU UUUGUUGGUU UCUAGGACCA UCGUAAUGAU UAAUAGGGAC GGUCGGGGGC AUCAGUAUU CAAUUGUCAG AGGUGAAAUU CUUGGAUUUA UUGAAGACUA ACUACUGCGA AAGCAUUUGC CAAGGACGUU U UCAUUAAU CAAGAACGAA AGUUAGGGGA UCGAAGAUGA UCAGAUACCG UCGUAGUCUU AACCAUAAAC UAUGCCGACU AG GGAUCGG GUGGUGUUUU UUUAAUGACC CACUCGGCAC CUUACGAGAA AUCAAAGUCU UUGGGUUCUG GGGGGAGUAU GGU CGCAAG GCUGAAACUU AAAGGAAUUG ACGGAAGGGC ACCACCAGGA GUGGAGCCUG CGGCUUAAUU UGACUCAACA CGGG GAAAC UCACCAGGUC CAGACACAAU AAGGAUUGAC AGAUUGAGAG CUCUUUCUUG AUUUUGUGGG UGGUGGUGCA UGGCC GUUC UUAGUUGGUG GAGUGAUUUG UCUGCUUAAU UGCGAUAACG AACGAGACCU UAACCUACUA AAUAGUGGUG CUAGCA UUU GCUGGUUAUC CACUUCUUAG AGGGACUAUC GGUUUCAAGC CGAUGGAAGU UUGAGGCAAU AACAGGUCUG UGAUGCC CU UAGACGUUCU GGGCCGCACG CGCGCUACAC UGACGGAGCC AGCGAGUCUA ACCUUGGCCG AGAGGUCUUG GUAAUCUU G UGAAACUCCG UCGUGCUGGG GAUAGAGCAU UGUAAUUAUU GCUCUUCAAC GAGGAAUUCC UAGUAAGCGC AAGUCAUCA GCUUGCGUUG AUUACGUCCC UGCCCUUUGU ACACACCGCC CGUCGCUAGU ACCGAUUGAA UGGCUUAGUG AGGCCUCAGG AUCUGCUUA GAGAAGGGGG CAACUCCAUC UCAGAGCGGA GAAUUUGGAC AAACUUGGUC AUUUAGAGGA ACUAAAAGUC G UAACAAGG UUUCCGUAGG UGAACCUGCG GAAGGAUCAU UA

Macromolecule #2: RNA (5'-R(P*AP*UP*GP*AP*AP*A)-3')

• Macromolecule: Name: RNA (5'-R(P*AP*UP*GP*AP*AP*A)-3') / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 1.923237 KDa

Sequence

String:

AUGAAA

Macromolecule #36: tRNA (76-MER)

• Macromolecule: Name: tRNA (76-MER) / type: rna / ID: 36 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 24.378408 KDa

Sequence

String:

GGGUCGUUAG CUCAGUUGGU AGAGCAGUUG ACUUUUAAUC AAUUGGUCGC AGGUUCGAAU CCUGCACGAC CCACCA

Macromolecule #37: tRNA (75-MER)

• Macromolecule: Name: tRNA (75-MER) / type: rna / ID: 37 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 24.2225 KDa

Sequence

String:

AGCGCCGUGA CGCAGUGGAA GCGCGCAGGG CUCAUAACCC UGAUGUCCUC GGAUCGAAAC CGAGCGGCGC UACCA

Macromolecule #40: 25S rRNA (3184-MER)

• Macromolecule: Name: 25S rRNA (3184-MER) / type: rna / ID: 40 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 1.096842375 MDa

Sequence

String:

UUGACCUCAA AUCAGGUAGG AGUACCCGCU GAACUUAAGC AUAUCAAUAA GCGGAGGAAA AGAAACCAAC CGGGAUUGCC UUAGUAACG GCGAGUGAAG CGGCAAAAGC UCAAAUUUGA AAUCUGGUAC CUUCGGUGCC CGAGUUGUAA UUUGGAGAGG G CAACUUUG GGGCCGUUCC UUGUCUAUGU UCCUUGGAAC AGGACGUCAU AGAGGGUGAG AAUCCCGUGU GGCGAGGAGU GC GGUUCUU UGUAAAGUGC CUUCGAAGAG UCGAGUUGUU UGGGAAUGCA GCUCUAAGUG GGUGGUAAAU UCCAUCUAAA GCU AAAUAU UGGCGAGAGA CCGAUAGCGA ACAAGUACAG UGAUGGAAAG AUGAAAAGAA CUUUGAAAAG AGAGUGAAAA AGUA CGUGA AAUUGUUGAA AGGGAAGGGC AUUUGAUCAG ACAUGGUGUU UUGUGCCCUC UGCUCCUUGU GGGUAGGGGA AUCUC GCAU UUCACUGGGC CAGCAUCAGU UUUGGUGGCA GGAUAAAUCC AUAGGAAUGU AGCUUGCCUC GGUAAGUAUU AUAGCC UGU GGGAAUACUG CCAGCUGGGA CUGAGGACUG CGACGUAAGU CAAGGAUGCU GGCAUAAUGG UUAUAUGCCG CCCGUCU UG AAACACGGAC CAAGGAGUCU AACGUCUAUG CGAGUGUUUG GGUGUAAAAC CCAUACGCGU AAUGAAAGUG AACGUAGG U UGGGGCCUCG CAAGAGGUGC ACAAUCGACC GAUCCUGAUG UCUUCGGAUG GAUUUGAGUA AGAGCAUAGC UGUUGGGAC CCGAAAGAUG GUGAACUAUG CCUGAAUAGG GUGAAGCCAG AGGAAACUCU GGUGGAGGCU CGUAGCGGUU CUGACGUGCA AAUCGAUCG UCGAAUUUGG GUAUAGGGGC GAAAGACUAA UCGAACCAUC UAGUAGCUGG UUCCUGCCGA AGUUUCCCUC A GGAUAGCA GAAGCUCGUA UCAGUUUUAU GAGGUAAAGC GAAUGAUUAG AGGUUCCGGG GUCGAAAUGA CCUUGACCUA UU CUCAAAC UUUAAAUAUG UAAGAAGUCC UUGUUACUUA AUUGAACGUG GACAUUUGAA UGAAGAGCUU UUAGUGGGCC AUU UUUGGU AAGCAGAACU GGCGAUGCGG GAUGAACCGA ACGUAGAGUU AAGGUGCCGG AAUACACGCU CAUCAGACAC CACA AAAGG UGUUAGUUCA UCUAGACAGC CGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAGGAGUGU GUAACAACUC ACCGG CCGA AUGAACUAGC CCUGAAAAUG GAUGGCGCUC AAGCGUGUUA CCUAUACUCU ACCGUCAGGG UUGAUAUGAU GCCCUG ACG AGUAGGCAGG CGUGGAGGUC AGUGACGAAG CCUAGACCGU AAGGUCGGGU CGAACGGCCU CUAGUGCAGA UCUUGGU GG UAGUAGCAAA UAUUCAAAUG AGAACUUUGA AGACUGAAGU GGGGAAAGGU UCCACGUCAA CAGCAGUUGG ACGUGGGU U AGUCGAUCCU AAGAGAUGGG GAAGCUCCGU UUCAAAGGCC UGAUUUUAUG CAGGCCACCA UCGAAAGGGA AUCCGGUUA AGAUUCCGGA ACCUGGAUAU GGAUUCUUCA CGGUAACGUA ACUGAAUGUG GAGACGUCGG CGCGAGCCCU GGGAGGAGUU AUCUUUUCU UCUUAACAGC UUAUCACCCC GGAAUUGGUU UAUCCGGAGA UGGGGUCUUA UGGCUGGAAG AGGCCAGCAC C UUUGCUGG CUCCGGUGCG CUUGUGACGG CCCGUGAAAA UCCACAGGAA GGAAUAGUUU UCAUGCCAGG UCGUACUGAU AA CCGCAGC AGGUCUCCAA GGUGAACAGC CUCUAGUUGA UAGAAUAAUG UAGAUAAGGG AAGUCGGCAA AAUAGAUCCG UAA CUUCGG GAUAAGGAUU GGCUCUAAGG GUCGGGUAGU GAGGGCCUUG GUCAGACGCA GCGGGCGUGC UUGUGGACUG CUUG GUGGG GCUUGCUCUG CUAGGCGGAC UACUUGCGUG CCUUGUUGUA GACGGCCUUG GUAGGUCUCU UGUAGACCGU CGCUU GCUA CAAUUAACGA UCAACUUAGA ACUGGUACGG ACAAGGGGAA UCUGACUGUC UAAUUAAAAC AUAGCAUUGC GAUGGU CAG AAAGUGAUGU UGACGCAAUG UGAUUUCUGC CCAGUGCUCU GAAUGUCAAA GUGAAGAAAU UCAACCAAGC GCGGGUA AA CGGCGGGAGU AACUAUGACU CUCUUAAGGU AGCCAAAUGC CUCGUCAUCU AAUUAGUGAC GCGCAUGAAU GGAUUAAC G AGAUUCCCAC UGUCCCUAUC UACUAUCUAG CGAAACCACA GCCAAGGGAA CGGGCUUGGC AGAAUCAGCG GGGAAAGAA GACCCUGUUG AGCUUGACUC UAGUUUGACA UUGUGAAGAG ACAUAGAGGG UGUAGAAUAA GUGGGAGCUU CGGCGCCAGU GAAAUACCA CUACCUUUAU AGUUUCUUUA CUUAUUCAAU GAAGCGGAGC UGGAAUUCAU UUUCCACGUU CUAGCAUUCA A GGUCCCAU UCGGGGCUGA UCCGGGUUGA AGACAUUGUC AGGUGGGGAG UUUGGCUGGG GCGGCACAUC UGUUAAACGA UA ACGCAGA UGUCCUAAGG GGGGCUCAUG GAGAACAGAA AUCUCCAGUA GAACAAAAGG GUAAAAGCCC CCUUGAUUUU GAU UUUCAG UGUGAAUACA AACCAUGAAA GUGUGGCCUA UCGAUCCUUU AGUCCCUCGG AAUUUGAGGC UAGAGGUGCC AGAA AAGUU ACCACAGGGA UAACUGGCUU GUGGCAGUCA AGCGUUCAUA GCGACAUUGC UUUUUGAUUC UUCGAUGUCG GCUCU UCCU AUCAUACCGA AGCAGAAUUC GGUAAGCGUU GGAUUGUUCA CCCACUAAUA GGGAACGUGA GCUGGGUUUA GACCGU CGU GAGACAGGUU AGUUUUACCC UACUGAUGAA UGUUACCGCA AUAGUAAUUG AACUUAGUAC GAGAGGAACA GUUCAUU CG GAUAAUUGGU UUUUGCGGCU GUCUGAUCAG GCAUUGCCGC GAAGCUACCA UCCGCUGGAU UAUGGCUGAA CGCCUCUA A GUCAGAAUCC AUGCUAGAAC GCGGUGAUUU CUUUGCUCCA CACAAUAUAG AUGGAUACGA AUAAGGCGUC CUUGUGGCG UCGCUGAACC AUAGCAGGCU AGCAACGGUG CACUUGGCGG AAAGGCCUUG GGUGCUUGCU GGCGAAUUGC AAUGUCAUUU UGCGUGGGG AUAAAUCAUU UGUAUACGAC UUAGAUGUAC AACGGGGUAU UGUAAGCAGU AGAGUAGCCU UGUUGUUACG A UCUGCUGA GAUUAAGCCU UUGUUGUCUG AUUUGU

Macromolecule #41: 5S rRNA (121-MER)

• Macromolecule: Name: 5S rRNA (121-MER) / type: rna / ID: 41 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 38.951105 KDa

Sequence

String:

GGUUGCGGCC AUAUCUACCA GAAAGCACCG UUUCCCGUCC GAUCAACUGU AGUUAAGCUG GUAAGAGCCU GACCGAGUAG UGUAGUGGG UGACCAUACG CGAAACUCAG GUGCUGCAAU CU

Macromolecule #42: 5.8S rRNA (158-MER)

• Macromolecule: Name: 5.8S rRNA (158-MER) / type: rna / ID: 42 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 50.682922 KDa

Sequence

String:

AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

Macromolecule #3: 40S ribosomal protein S0-A

• Macromolecule: Name: 40S ribosomal protein S0-A / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 22.811074 KDa

Sequence

String:

SLPATFDLTP EDAQLLLAAN THLGARNVQV HQEPYVFNAR PDGVHVINVG KTWEKLVLAA RIIAAIPNPE DVVAISSRTF GQRAVLKFA AHTGATPIAG RFTPGSFTNY ITRSFKEPRL VIVTDPRSDA QAIKEASYVN IPVIALTDLD SPSEFVDVAI P CNNRGKHS IGLIWYLLAR EVLRLRGALV DRTQPWSIMP DLYFYRDP

Macromolecule #4: 40S ribosomal protein S1-A

• Macromolecule: Name: 40S ribosomal protein S1-A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 26.314684 KDa

Sequence

String:

AVGKNKRLSK GKKGQKKRVV DPFTRKEWFD IKAPSTFENR NVGKTLVNKS TGLKSASDAL KGRVVEVCLA DLQGSEDHSF RKIKLRVDE VQGKNLLTNF HGMDFTTDKL RSMVRKWQTL IEANVTVKTS DDYVLRIFAI AFTRKQANQV KRHSYAQSSH I RAIRKVIS EILTKEVQGS TLAQLTSKLI PEVINKEIEN ATKDIFPLQN IHVRKVKLLK QPKFDVGALM ALHG

Macromolecule #5: 40S ribosomal protein S15

• Macromolecule: Name: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 13.265591 KDa

Sequence

String:

KTHSYRGVDL EKLLEMSTED FVKLAPARVR RRFARGMTSK PAGFMKKLRA AKLAAPENEK PAPVRTHMRN MIIVPEMIGS VVGIYNGKA FNQVEIRPEM LGHYLGEFSI TYTPVRHG

Macromolecule #6: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 23.08485 KDa

Sequence

String:

GWVPVTKLGR LVKAGKITTI EEIFLHSLPV KEFQIIDTLL PGLQDEVMNI KPVQKQTRAG QRTRFKAVVV VGDSNGHVGL GIKTAKEVA GAIRAGIIIA KLSVIPIRRG YWGTNLGQPH SLATKTTGKC GSVTVRLIPA PRGSGIVASP AVKKLLQLAG V EDVYTQSN GKTRTLENTL KAAFVAIGNT YGFLTPNLWA EQPLPVSPLD IYSDEASA

Macromolecule #7: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 24.631713 KDa

Sequence

String:

LISKKRKLVA DGVFYAELNE FFTRELAEEG YSGVEVRVTP TKTEVIIRAT RTQDVLGENG RRINELTLLV QKRFKYAPGT IVLYAERVQ DRGLSAVAQA ESMKFKLLNG LAIRRAAYGV VRYVMESGAK GCEVVVSGKL RAARAKAMKF ADGFLIHSGQ P VNDFIDTA TRHVLMRQGV LGIKVKIMRD PAKSRTGPKA LPDAVTIIEP KEEEPILAPS VKDY

Macromolecule #8: 40S ribosomal protein S4-A

• Macromolecule: Name: 40S ribosomal protein S4-A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 29.167926 KDa

Sequence

String:

ARGPKKHLKR LAAPHHWLLD KLSGCYAPRP SAGPHKLRES LPLIVFLRNR LKYALNGREV KAILMQRHVK VDGKVRTDTT YPAGFMDVI TLDATNENFR LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP DPNIKVNDTV K IDLASGKI TDFIKFDAGK LVYVTGGRNL GRIGTIVHKE RHDGGFDLVH IKDSLDNTFV TRLNNVFVIG EQGKPYISLP KG KGIKLSI AEERDRRRAQ Q

Macromolecule #9: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 22.908338 KDa

Sequence

String:

FTPVVLATPI PEEVQQAQTE IKLFNKWSFE EVEVKDASLV DYVQVRQPIF VAHTAGRYAN KRFRKAQCPI IERLTNSLMM NGRNNGKKL KAVRIIKHTL DIINVLTDQN PIQVVVDAIT NTGPREDTTR VGGGGAARRQ AVDVSPLRRV NQAIALLTIG A REAAFRNI KTIAETLAEE LINAAKGSST SYAIKKKDEL ERVAKSNR

Macromolecule #10: 40S ribosomal protein S6-A

• Macromolecule: Name: 40S ribosomal protein S6-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 26.181445 KDa

Sequence

String:

MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE RKAEKAEIRK

Macromolecule #11: 40S ribosomal protein S7-A

• Macromolecule: Name: 40S ribosomal protein S7-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 21.000492 KDa

Sequence

String:

PQAKILSQAP TELELQVAQA FVELENSSPE LKAELRPLQF KSIREIDVAG GKKALAIFVP VPSLAGFHKV QTKLTRELEK KFQDRHVIF LAERRILPKP SRTSRQVQKR PRSRTLTAVH DKILEDLVFP TEIVGKRVRY LVGGNKIQKV LLDSKDVQQI D YKLESFQA VYNKLTGKQI VFEIPS

Macromolecule #12: 40S ribosomal protein S8-A

• Macromolecule: Name: 40S ribosomal protein S8-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 22.27743 KDa

Sequence

String:

GISRDSRHKR SATGAKRAQF RKKRKFELGR QPANTKIGAK RIHSVRTRGG NKKYRALRIE TGNFSWASEG ISKKTRIAGV VYHPSNNEL VRTNTLTKAA IVQIDATPFR QWFEAHYGQT LGKKKNVKEE ETVAKSKNAE RKWAARAASA KIESSVESQF S AGRLYACI SSRPGQSGRC DGYILEGEEL AFYLRRLTAK

Macromolecule #13: 40S ribosomal protein S9-A

• Macromolecule: Name: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 21.081549 KDa

Sequence

String:

PRAPRTYSKT YSTPKRPYES SRLDAELKLA GEFGLKNKKE IYRISFQLSK IRRAARDLLT RDEKDPKRLF EGNALIRRLV RVGVLSEDK KKLDYVLALK VEDFLERRLQ TQVYKLGLAK SVHHARVLIT QRHIAVGKQI VNIPSFMVRL DSEKHIDFAP T SPFGGARP GRVARRNAAR KAEASG

Macromolecule #14: 40S ribosomal protein S10-A

• Macromolecule: Name: 40S ribosomal protein S10-A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 11.042562 KDa

Sequence

String:

MLMPKEDRNK IHQYLFQEGV VVAKKDFNQA KHEEIDTKNL YVIKALQSLT SKGYVKTQFS WQYYYYTLTE EGVEYLREYL NLPEHIVPG TYI

Macromolecule #15: 40S ribosomal protein S11-A

• Macromolecule: Name: 40S ribosomal protein S11-A / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 16.272149 KDa

Sequence

String:

ELTVQSERAF QKQPHIFNNP KVKTSKRTKR WYKNAGLGFK TPKTAIEGSY IDKKCPFTGL VSIRGKILTG TVVSTKMHRT IVIRRAYLH YIPKYNRYEK RHKNVPVHVS PAFRVQVGDI VTVGQCRPIS KTVRFNVVKV SAA

Macromolecule #16: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 13.028008 KDa

Sequence

String:

TIEDALKVVL RTALVHDGLA RGLRESTKAL TRGEALLVVL VSSVTEANII KLVEGLANDP ENKVPLIKVA DAKQLGEWAG LGKIDREGN ARKVVGASVV VVKNWGAETD ELSMIMEHFS QQ

Macromolecule #17: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 16.928748 KDa

Sequence

String:

GRMHSAGKGI SSSAIPYSRN APAWFKLSSE SVIEQIVKYA RKGLTPSQIG VLLRDAHGVT QARVITGNKI MRILKSNGLA PEIPEDLYY LIKKAVSVRK HLERNRKDKD AKFRLILIES RIHRLARYYR TVAVLPPNWK YESATASALV N

Macromolecule #18: 40S ribosomal protein S14-B

• Macromolecule: Name: 40S ribosomal protein S14-B / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 13.446426 KDa

Sequence

String:

SQVFGVARIY ASFNDTFVHV TDLSGKETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH VKIRATGGTR TKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

Macromolecule #19: 40S ribosomal protein S16-A

• Macromolecule: Name: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 15.659216 KDa

Sequence

String:

AVPSVQTFGK KKSATAVAHV KAGKGLIKVN GSPITLVEPE ILRFKVYEPL LLVGLDKFSN IDIRVRVTGG GHVSQVYAIR QAIAKGLVA YHQKYVDEQS KNELKKAFTS YDRTLLIADS RRPEPKKFGG KGARSRFQKS YR

Macromolecule #20: 40S ribosomal protein S17-B

• Macromolecule: Name: 40S ribosomal protein S17-B / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 14.268545 KDa

Sequence

String:

GRVRTKTVKR ASKALIERYY PKLTLDFQTN KRLCDEIATI QSKRLRNKIA GYTTHLMKRI QKGPVRGISF KLQEEERERK DQYVPEVSA LDLSRSNGVL NVDNQTSDLV KSLGLKLPLS VINVSA

Macromolecule #21: 40S ribosomal protein S18-A

• Macromolecule: Name: 40S ribosomal protein S18-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 16.940443 KDa

Sequence

String:

SLVVQEQGSF QHILRLLNTN VDGNIKIVYA LTTIKGVGRR YSNLVCKKAD VDLHKRAGEL TQEELERIVQ IMQNPTHYKI PAWFLNRQN DITDGKDYHT LANNVESKLR DDLERLKKIR AHRGIRHFWG LRVRGQHTKT TGRRRA

Macromolecule #22: 40S ribosomal protein S19-A

• Macromolecule: Name: 40S ribosomal protein S19-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 15.81093 KDa

Sequence

String:

PGVSVRDVAA QDFINAYASF LQRQGKLEVP GYVDIVKTSS GNEMPPQDAE GWFYKRAASV ARHIYMRKQV GVGKLNKLYG GAKSRGVRP YKHIDASGSI NRKVLQALEK IGIVEISPKG GRRISENGQR DLDRIAAQTL EEDE

Macromolecule #23: 40S ribosomal protein S20

• Macromolecule: Name: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 11.363321 KDa

Sequence

String:

IKIRITLTST KVKQLENVSS NIVKNAEQHN LVKKGPVRLP TKVLKISTRK TPNGEGSKTW ETYEMRIHKR YIDLEAPVQI VKRITQITI EPGVDVEVVV A

Macromolecule #24: 40S ribosomal protein S21-A

• Macromolecule: Name: 40S ribosomal protein S21-A / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 9.758829 KDa

Sequence

String:

MENDKGQLVE LYVPRKCSAT NRIIKADDHA SVQINVAKVD EEGRAIPGEY VTYALSGYVR SRGESDDSLN RLAQNDGLLK NVWSYSR

Macromolecule #25: 40S ribosomal protein S22-A

• Macromolecule: Name: 40S ribosomal protein S22-A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 14.518867 KDa

Sequence

String:

TRSSVLADAL NAINNAEKTG KRQVLIRPSS KVIIKFLQVM QKHGYIGEFE YIDDHRSGKI VVQLNGRLNK CGVISPRFNV KIGDIEKWT ANLLPARQFG YVILTTSAGI MDHEEARRKH VSGKILGFVY

Macromolecule #26: 40S ribosomal protein S23-A

• Macromolecule: Name: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 15.942699 KDa

Sequence

String:

GKGKPRGLNS ARKLRVHRRN NRWAENNYKK RLLGTAFKSS PFGGSSHAKG IVLEKLGIES KQPNSAIRKC VRVQLIKNGK KVTAFVPND GCLNFVDEND EVLLAGFGRK GKAKGDIPGV RFKVVKVSGV SLLALWKEKK EKPRS

Macromolecule #27: 40S ribosomal protein S24-A

• Macromolecule: Name: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 15.23165 KDa

Sequence

String:

SDAVTIRTRK VISNPLLARK QFVVDVLHPN RANVSKDELR EKLAEVYKAE KDAVSVFGFR TQFGGGKSVG FGLVYNSVAE AKKFEPTYR LVRYGLAEKV EKASRQQRKQ KKNRDKKIFG TGKRLAKKVA RRNAD

Macromolecule #28: 40S ribosomal protein S25-A

• Macromolecule: Name: 40S ribosomal protein S25-A / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 9.540301 KDa

Sequence

String:

KKKWSKKSMK DRAQHAVILD QEKYDRILKE VPTYRYVSVS VLVDRLKIGG SLARIALRHL EKEGIIKPIS KHSKQAIYTR AT

Macromolecule #29: 40S ribosomal protein S26-B

• Macromolecule: Name: 40S ribosomal protein S26-B / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 11.022989 KDa

Sequence

String:

PKKRASNGRN KKGRGHVKPV RCVNCSKSIP KDKAIKRMAI RNIVEAAAVR DLSEASVYPE YALPKTYNKL HYCVSCAIHA RIVRVRSRE DRKNRAPP

Macromolecule #30: 40S ribosomal protein S27-A

• Macromolecule: Name: 40S ribosomal protein S27-A / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 8.762195 KDa

Sequence

String:

VLVQDLLHPT AASEARKHKL KTLVQGPRSY FLDVKCPGCL NITTVFSHAQ TAVTCESCST ILCTPTGGKA KLSEGTSFRR K

Macromolecule #31: 40S ribosomal protein S29-A

• Macromolecule: Name: 40S ribosomal protein S29-A / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 6.335303 KDa

Sequence

String:

ENVWFSHPRR YGKGSRQCRV CSSHTGLIRK YGLNICRQCF REKANDIGFN KFR

Macromolecule #32: 40S ribosomal protein S30-A

• Macromolecule: Name: 40S ribosomal protein S30-A / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 6.479698 KDa

Sequence

String:

HGSLARAGKV KSQTPKVEKT EKPKKPKGRA YKRLLYTRRF VNVTLVNGKR RMNPGPS

Macromolecule #33: 40S ribosomal protein S31

• Macromolecule: Name: 40S ribosomal protein S31 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 8.329946 KDa

Sequence

String:

RKKKVYTTPK KIKHKHKAVK LAVLSYYKVD AEGKVTKLRR ECSNPTCGAG VFLANHKDRL YCGKCHSVYK VNA

Macromolecule #34: Guanine nucleotide-binding protein subunit beta-like protein

• Macromolecule: Name: Guanine nucleotide-binding protein subunit beta-like protein; type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 34.151484 KDa

Sequence

String:

EVLVLRGTLE GHNGWVTSLA TSAGQPNLLL SASRDKTLIS WKLTGDDQKF GVPVRSFKGH SHIVQDCTLT ADGAYALSAS WDKTLRLWD VATGETYQRF VGHKSDVMSV DIDKKASMII SGSRDKTIKV WTIKGQCLAT LLGHNDWVSQ VRVVPNEKAD D DSVTIISA GNDKMVKAWN LNQFQIEADF IGHNSNINTL TASPDGTLIA SAGKDGEIML WNLAAKKAMY TLSAQDEVFS LA FSPNRYW LAAATATGIK VFSLDPQYLV DDLRPEFAGY SKAAEPHAVS LAWSADGQTL FAGYTDNVIR VWQVM

Macromolecule #35: 40S ribosomal protein S28-A

• Macromolecule: Name: 40S ribosomal protein S28-A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 7.116281 KDa

Sequence

String:

TPVTLAKVIK VLGRTGSRGG VTQVRVEFLE DTSRTIVRNV KGPVRENDIL VLMESEREAR RLR

Macromolecule #38: Ribosome-interacting GTPase 2

• Macromolecule: Name: Ribosome-interacting GTPase 2 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 40.536207 KDa

Sequence

String:

GIIDKIKAIE EEMARTQKNK ATEHHLGLLK GKLARYRQQL LADEAGSGGG GGSGFEVAAS GDARVVLIGY PSVGKSSLLG KITTTKSEA AEYEFTTLTS VPGVLKYQGA EIQIVDLPGI IYGASQGKGR GKQVVATART ADLVLMVLDA TKSEHQRASL E KELENVGI RLNKEKPNIY YKKKETGGVK VTFTSPPKTN LTEQAIKMIL RDYRIHNAEV LVRDDQCTID DFIDVINEQH RN YVKCLYV YNKIDAVSLE EVDKLAREPN AVVMSCEMDL GLQDVVEEIW YQLNLSRVAT KKAGVRPVFD DPLVVRNDST IGD LCHGIH RDFKDKFKYA LVWGSSAKHS PQKCGLNHRI EDEDVVSLFA

Macromolecule #39: GIR2

• Macromolecule: Name: GIR2 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 4.103049 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #43: 60S ribosomal protein L2-A

• Macromolecule: Name: 60S ribosomal protein L2-A / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 27.089158 KDa

Sequence

String:

GRVIRNQRKG AGSIFTSHTR LRQGAAKLRT LDYAERHGYI RGIVKQIVHD SGRGAPLAKV VFRDPYKYRL REEIFIANEG VHTGQFIYA GKKASLNVGN VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL PSGAKKVISS D ARGVIGVI AGGGRVDKPL LKAGRAFHKY RLKRNSWPKT RGVAMNPVDH PHGGGNHQHI GKASTISRGA VSGQKAGLIA AR RTGLLRG SQKT

Macromolecule #44: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 43.719602 KDa

Sequence

String:

SHRKYEAPRH GHLGFLPRKR AASIRARVKA FPKDDRSKPV ALTSFLGYKA GMTTIVRDLD RPGSKFHKRE VVEAVTVVDT PPVVVVGVV GYVETPRGLR SLTTVWAEHL SDEVKRRFYK NWYKSKKKAF TKYSAKYAQD GAGIERELAR IKKYASVVRV L VHTQIRKT PLAQKKAHLA EIQLNGGSIS EKVDWAREHF EKTVAVDSVF EQNEMIDAIA VTKGHGFEGV THRWGTKKLP RK THRGLRK VACIGAWHPA HVMWSVARAG QRGYHSRTSI NHKIYRVGKG DDEANGATSF DRTKKTITPM GGFVHYGEIK NDF IMVKGC IPGNRKRIVT LRKSLYTNTS RKALEEVSLK WIDTASKFGK GRFQTPAEKH AFMGTLKKDL

Macromolecule #45: 60S ribosomal protein L4-A

• Macromolecule: Name: 60S ribosomal protein L4-A / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 39.027926 KDa

Sequence

String:

SRPQVTVHSL TGEATANALP LPAVFSAPIR PDIVHTVFTS VNKNKRQAYA VSEKAGHQTS AESWGTGRAV ARIPRVGGGG TGRSGQGAF GNMCRGGRMF APTKTWRKWN VKVNHNEKRY ATASAIAATA VASLVLARGH RVEKIPEIPL VVSTDLESIQ K TKEAVAAL KAVGAHSDLL KVLKSKKLRA GKGKYRNRRW TQRRGPLVVY AEDNGIVKAL RNVPGVETAN VASLNLLQLA PG AHLGRFV IWTEAAFTKL DQVWGSETVA SSKVGYTLPS HIISTSDVTR IINSSEIQSA IRPAGQATQK RTHVLKKNPL KNK QVLLRL NPYAKVFAAE KLGSKKAEKT GTKPAAVFTE TLKHD

Macromolecule #46: 60S ribosomal protein L5

• Macromolecule: Name: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 33.415387 KDa

Sequence

String:

QKDAKSSAYS SRFQTPFRRR REGKTDYYQR KRLVTQHKAK YNTPKYRLVV RFTNKDIICQ IISSTITGDV VLAAAYSHEL PRYGITHGL TNWAAAYATG LLIARRTLQK LGLDETYKGV EEVEGEYELT EAVEDGPRPF KVFLDIGLQR TTTGARVFGA L KGASDGGL YVPHSENRFP GWDFETEEID PELLRSYIFG GHVSQYMEEL ADDDEERFSE LFKGYLADDI DADSLEDIYT SA HEAIRAD PAFKPTEKKF TKEQYAAESK KYRQTKLSKE ERAARVAAKI AALAGQQ

Macromolecule #47: 60S ribosomal protein L6-B

• Macromolecule: Name: 60S ribosomal protein L6-B / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 19.908422 KDa

Sequence

String:

TAQQAPKWYP SEDVAAPKKT RKAVRPQKLR ASLVPGTVLI LLAGRFRGKR VVYLKHLEDN TLLVTGPFKV NGVPLRRVNA RYVIATSTK VSVEGVNVEK FNVEYFAKEK LTKKEKKEAK LFPEQQTKEI KTERVEDQKV VDKALLAEIK KTPLLKQYLS A SFSLKNGD KPHLLKF

Macromolecule #48: 60S ribosomal protein L7-A

• Macromolecule: Name: 60S ribosomal protein L7-A / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 25.240377 KDa

Sequence

String:

AEQVAAERAA RKAANKEKRA IILERNAAYQ KEYETAERNI IQAKRDAKAA GSYYVEAQHK LVFVVRIKGI NKIPPKPRKV LQLLRLTRI NSGTFVKVTK ATLELLKLIE PYVAYGYPSY STIRQLVYKR GFGKINKQRV PLSDNAIIEA NLGKYGILSI D DLIHEIIT VGPHFKQANN FLWPFKLSNP SGGWGVPRKF KHFIQGGSFG NREEFINKLV KSMN

Macromolecule #49: 60S ribosomal protein L8-A

• Macromolecule: Name: 60S ribosomal protein L8-A / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 25.814998 KDa

Sequence

String:

NPLTHSTPKN FGIGQAVQPK RNLSRYVKWP EYVRVQRQKK ILSIRLKVPP TIAQFQYTLD RNTAAETFKL FNKYRPETAA EKKERLTKE AAAVAEGKSK QDASPKPYAV KYGLNHVVAL IENKKAKLVL IANDVDPIEL VVFLPALCKK MGVPYAIVKG K ARLGTLVN QKTSAVAALT EVRAEDEAAL AKLVSTIDAN FADKYDEVKK HWGGGILGNK AQAKMDKRAK NSDSA

Macromolecule #50: 60S ribosomal protein L9-A

• Macromolecule: Name: 60S ribosomal protein L9-A / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 21.605061 KDa

Sequence

String:

MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

Macromolecule #51: 60S ribosomal protein L10

• Macromolecule: Name: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 25.080062 KDa

Sequence

String:

ARRPARCYRY QKNKPYPKSR YNRAVPDSKI RIYDLGKKKA TVDEFPLCVH LVSNELEQLS SEALEAARIC ANKYMTTVSG RDAFHLRVR VHPFHVLRIN KMLSCAGADR LQQGMRGAWG KPHGLAARVD IGQIIFSVRT KDSNKDVVVE GLRRARYKFP G QQKIILSK KWGFTNLDRP EYLKKREAGE VKDDGAFVKF LSKKGSLENN IREFPEYFAA

Macromolecule #52: 60S ribosomal protein L11-B

• Macromolecule: Name: 60S ribosomal protein L11-B / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 19.266082 KDa

Sequence

String:

QNPMRDLKIE KLVLNISVGE SGDRLTRASK VLEQLSGQTP VQSKARYTVR TFGIRRNEKI AVHVTVRGPK AEEILERGLK VKEYQLRDR NFSATGNFGF GIDEHIDLGI KYDPSIGIFG MDFYVVMNRP GARVTRRKRC KGTVGNSHKT TKEDTVSWFK Q KYDADVLD K

Macromolecule #53: 60S ribosomal protein L13-A

• Macromolecule: Name: 60S ribosomal protein L13-A / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 21.885234 KDa

Sequence

String:

AISKNLPILK NHFRKHWQER VKVHFDQAGK KVSRRNARAT RAAKIAPRPL DLLRPVVRAP TVKYNRKVRA GRGFTLAEVK AAGLTAAYA RTIGIAVDHR RQNRNQEIFD ANVQRLKEYQ SKIIVFPRNG KAPEAEQVLS AAATFPIAQP ATDVEARAVQ D NGESAFRT LRLARSEKKF RGIREKRARE KAEAE

Macromolecule #54: 60S ribosomal protein L14-A

• Macromolecule: Name: 60S ribosomal protein L14-A / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 14.976794 KDa

Sequence

String:

TDSIVKASNW RLVEVGRVVL IKKGQSAGKL AAIVEIIDQK KVLIDGPKAG VPRQAINLGQ VVLTPLTFAL PRGARTATVS KKWAAAAVC EKWAASSWAK KIAQRERRAA LTDFERFQVM VLRKQKRYTV KKALAKA

Macromolecule #55: 60S ribosomal protein L15-A

• Macromolecule: Name: 60S ribosomal protein L15-A / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 24.351162 KDa

Sequence

String:

GAYKYLEELQ RKKQSDVLRF LQRVRVWEYR QKNVIHRAAR PTRPDKARRL GYKAKQGFVI YRVRVRRGNR KRPVPKGATY GKPTNQGVN ELKYQRSLRA TAEERVGRRA ANLRVLNSYW VNQDSTYKYF EVILVDPQHK AIRRDARYNW ICDPVHKHRE A RGLTATGK KSRGINKGHK FNNTKAGRRK TWKRQNTLSL WRYRK

Macromolecule #56: 60S ribosomal protein L16-A

• Macromolecule: Name: 60S ribosomal protein L16-A / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 22.028951 KDa

Sequence

String:

VEPVVVIDGK GHLVGRLASV VAKQLLNGQK IVVVRAEELN ISGEFFRNKL KYHDFLRKAT AFNKTRGPFH FRAPSRIFYK ALRGMVSHK TARGKAALER LKVFEGIPPP YDKKKRVVVP QALRVLRLKP GRKYTTLGKL STSVGWKYED VVAKLEAKRK V SSAEYYAK KRAFTKKVAS ANATAAESDV AKQLAALGY

Macromolecule #57: 60S ribosomal protein L17-A

• Macromolecule: Name: 60S ribosomal protein L17-A / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 20.458322 KDa

Sequence

String:

ARYGATSTNP AKSASARGSY LRVSFKNTRE TAQAINGWEL TKAQKYLEQV LDHQRAIPFR RFNSSIGRTA QGKEFGVTKA RWPAKSVKF VQGLLQNAAA NAEAKGLDAT KLYVSHIQVN QAPKQRRRTY RAHGRINKYE SSPSHIELVV TEKEEAVAKA A EKKVVRLT SRQRGRIAAQ KRIAA

Macromolecule #58: 60S ribosomal protein L18-A

• Macromolecule: Name: 60S ribosomal protein L18-A / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 20.478059 KDa

Sequence

String:

GIDHTSKQHK RSGHRTAPKS DNVYLKLLVK LYTFLARRTD APFNKVVLKA LFLSKINRPP VSVSRIARAL KQEGAANKTV VVVGTVTDD ARIFEFPKTT VAALRFTAGA RAKIVKAGGE CITLDQLAVR APKGQNTLIL RGPRNSREAV RHFGMGPHKG K APRILSTG RKFERARGRR RSKGFKV

Macromolecule #59: 60S ribosomal protein L19-A

• Macromolecule: Name: 60S ribosomal protein L19-A / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 21.631121 KDa

Sequence

String:

ANLRTQKRLA ASVVGVGKRK VWLDPNETSE IAQANSRNAI RKLVKNGTIV KKAVTVHSKS RTRAHAQSKR EGRHSGYGKR KGTREARLP SQVVWIRRLR VLRRLLAKYR DAGKIDKHLY HVLYKESKGN AFKHKRALVE HIIQAKADAQ REKALNEEAE A RRLKNRAA RDRRAQRVAE KRDALLKEDA

Macromolecule #60: 60S ribosomal protein L20-A

• Macromolecule: Name: 60S ribosomal protein L20-A / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 20.347654 KDa

Sequence

String:

AHFKEYQVIG RRLPTESVPE PKLFRMRIFA SNEVIAKSRY WYFLQKLHKV KKASGEIVSI NQINEAHPTK VKNFGVWVRY DSRSGTHNM YKEIRDVSRV AAVETLYQDM AARHRARFRS IHILKVAEIE KTADVKRQYV KQFLTKDLKF PLPHRVQKST K TFSYKRPS TFY

Macromolecule #61: 60S ribosomal protein L21-A

• Macromolecule: Name: 60S ribosomal protein L21-A / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 18.148066 KDa

Sequence

String:

GKSHGYRSRT RYMFQRDFRK HGAVHLSTYL KVYKVGDIVD IKANGSIQKG MPHKFYQGKT GVVYNVTKSS VGVIINKMVG NRYLEKRLN LRVEHIKHSK CRQEFLERVK ANAAKRAEAK AQGVAVQLKR QPAQPRESRI VSTEGNVPQT LAPVPYETFI

Macromolecule #62: 60S ribosomal protein L22-A

• Macromolecule: Name: 60S ribosomal protein L22-A / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 11.263863 KDa

Sequence

String:

QKIAKTFTVD VSSPTENGVF DPASYAKYLI DHIKVEGAVG NLGNAVTVTE DGTVVTVVST AKFSGKYLKY LTKKYLKKNQ LRDWIRFVS TKTNEYRLAF Y

Macromolecule #63: 60S ribosomal protein L23-A

• Macromolecule: Name: 60S ribosomal protein L23-A / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 14.362755 KDa

Sequence

String:

SGNGAQGTKF RISLGLPVGA IMNCADNSGA RNLYIIAVKG SGSRLNRLPA ASLGDMVMAT VKKGKPELRK KVMPAIVVRQ AKSWRRRDG VFLYFEDNAG VIANPKGEMK GSAITGPVGK ECADLWPRVA SNSGVVV

Macromolecule #64: 60S ribosomal protein L24-A

• Macromolecule: Name: 60S ribosomal protein L24-A / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 7.614926 KDa

Sequence

String:

MKVEIDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK KGITE

Macromolecule #65: 60S ribosomal protein L25

• Macromolecule: Name: 60S ribosomal protein L25 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 13.771176 KDa

Sequence

String:

KALKVRTSAT FRLPKTLKLA RAPKYASKAV PHYNRLDSYK VIEQPITSET AMKKVEDGNI LVFQVSMKAN KYQIKKAVKE LYEVDVLKV NTLVRPNGTK KAYVRLTADY DALDIANRIG YI

Macromolecule #66: 60S ribosomal protein L26-A

• Macromolecule: Name: 60S ribosomal protein L26-A / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 14.005475 KDa

Sequence

String:

AKQSLDVSSD RRKARKAYFT APSSQRRVLL SAPLSKELRA QYGIKALPIR RDDEVLVVRG SKKGQEGKIS SVYRLKFAVQ VDKVTKEKV NGASVPINLH PSKLVITKLH LDKDRKALIQ RKGGKL

Macromolecule #67: 60S ribosomal protein L27-A

• Macromolecule: Name: 60S ribosomal protein L27-A / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 15.437166 KDa

Sequence

String:

AKFLKAGKVA VVVRGRYAGK KVVIVKPHDE GSKSHPFGHA LVAGIERYPL KVTKKHGAKK VAKRTKIKPF IKVVNYNHLL PTRYTLDVE AFKSVVSTET FEQPSQREEA KKVVKKAFEE RHQAGKNQWF FSKLRF

Macromolecule #68: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 16.630471 KDa

Sequence

String:

PSRFTKTRKH RGHVSAGKGR IGKHRKHPGG RGMAGGQHHH RINMDKYHPG YFGKVGMRYF HKQQAHFWKP VLNLDKLWTL IPEDKRDQY LKSASKETAP VIDTLAAGYG KILGKGRIPN VPVIVKARFV SKLAEEKIRA AGGVVELIA

Macromolecule #69: 60S ribosomal protein L29

• Macromolecule: Name: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 6.560688 KDa

Sequence

String:

AKSKNHTAHN QTRKAHRNGI KKPKTYKYPS LKGVDPKFRR NHKHALHGTA KALAAAKK

Macromolecule #70: 60S ribosomal protein L30

• Macromolecule: Name: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 10.487266 KDa

Sequence

String:

SINQKLALVI KSGKYTLGYK STVKSLRQGK SKLIIIAANT PVLRKSELEY YAMLSKTKVY YFQGGNNELG TAVGKLFRVG VVSILEAGD SDILTTL

Macromolecule #71: 60S ribosomal protein L31-A

• Macromolecule: Name: 60S ribosomal protein L31-A / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 12.649754 KDa

Sequence

String:

LKDVVTREYT INLHKRLHGV SFKKRAPRAV KEIKKFAKLH MGTDDVRLAP ELNQAIWKRG VKGVEYRLRL RISRKRNEEE DAKNPLFSY VEPVLVASAK GLQTVVVEED

Macromolecule #72: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 14.478054 KDa

Sequence

String:

ASLPHPKIVK KHTKKFKRHH SDRYHRVAEN WRKQKGIDSV VRRRFRGNIS QPKIGYGSNK KTKFLSPSGH KTFLVANVKD LETLTMHTK TYAAEIAHNI SAKNRVVILA RAKALGIKVT NPKGRLAL

Macromolecule #73: 60S ribosomal protein L33-A

• Macromolecule: Name: 60S ribosomal protein L33-A / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 12.045935 KDa

Sequence

String:

AESHRLYVKG KHLSYQRSKR VNNPNVSLIK IEGVATPQDA QFYLGKRIAY VYRASKEVRG SKIRVMWGKV TRTHGNSGVV RATFRNNLP AKTFGASVRI FLYPSNI

Macromolecule #74: 60S ribosomal protein L34-A

• Macromolecule: Name: 60S ribosomal protein L34-A / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 12.608831 KDa

Sequence

String:

AQRVTFRRRN PYNTRSNKIK VVKTPGGILR AQHVKKLATR PKCGDCGSAL QGISTLRPRQ YATVSKTHKT VSRAYGGSRC ANCVKERII RAFLIEEQKI VKKVVKEQTE AAK

Macromolecule #75: 60S ribosomal protein L35-A

• Macromolecule: Name: 60S ribosomal protein L35-A / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 13.811444 KDa

Sequence

String:

AGVKAYELRT KSKEQLASQL VDLKKELAEL KVQKLSRPSL PKIKTVRKSI ACVLTVINEQ QREAVRQLYK GKKYQPKDLR AKKTRALRR ALTKFEASQV TEKQRKKQIA FPQRKYAIKA

Macromolecule #76: 60S ribosomal protein L36-A

• Macromolecule: Name: 60S ribosomal protein L36-A / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 11.020063 KDa

Sequence

String:

TVKTGIAIGL NKGKKVTSMT PAPKISYKKG AASNRTKFVR SLVREIAGLS PYERRLIDLI RNSGEKRARK VAKKRLGSFT RAKAKVEEM NNIIAASRRH

Macromolecule #77: 60S ribosomal protein L37-A

• Macromolecule: Name: 60S ribosomal protein L37-A / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 9.229632 KDa

Sequence

String:

GKGTPSFGKR HNKSHTLCNR CGRRSFHVQK KTCSSCGYPA AKTRSYNWGA KAKRRHTTGT GRMRYLKHVS RRFKNGFQTG S

Macromolecule #78: 60S ribosomal protein L38

• Macromolecule: Name: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 8.714363 KDa

Sequence

String:

AREITDIKQF LELTRRADVK TATVKINKKL NKAGKPFRQT KFKVRGSSSL YTLVINDAGK AKKLIQSLPP TLKVNRL

Macromolecule #79: 60S ribosomal protein L39

• Macromolecule: Name: 60S ribosomal protein L39 / type: protein_or_peptide / ID: 79 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 6.227444 KDa

Sequence

String:

AAQKSFRIKQ KMAKAKKQNR PLPQWIRLRT NNTIRYNAKR RNWRRTKMNI

Macromolecule #80: 60S ribosomal protein L40-A

• Macromolecule: Name: 60S ribosomal protein L40-A / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 6.032321 KDa

Sequence

String:

IIEPSLKALA SKYNCDKSVC RKCYARLPPR ATNCRKRKCG HTNQLRPKKK LK

Macromolecule #81: 60S ribosomal protein L41-A

• Macromolecule: Name: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 3.354243 KDa

Sequence

String:

MRAKWRKKRT RRLKRKRRKV RARSK

Macromolecule #82: 60S ribosomal protein L42-A

• Macromolecule: Name: 60S ribosomal protein L42-A / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 11.840159 KDa

Sequence

String:

VNVPKTRKTY CKGKTCRKHT QHKVTQYKAG KASLFAQGKR RYDRKQSGFG GQTKPVFHKK AKTTKKVVLR LECVKCKTRA QLTLKRCKH FELGGEKKQK GQAL

Macromolecule #83: 60S ribosomal protein L43-A

• Macromolecule: Name: 60S ribosomal protein L43-A / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 9.981756 KDa

Sequence

String:

AKRTKKVGIT GKYGVRYGSS LRRQVKKLEI QQHARYDCSF CGKKTVKRGA AGIWTCSCCK KTVAGGAYTV STAAAATVRS TIRRLREMV EA

Macromolecule #84: eiF5A

• Macromolecule: Name: eiF5A / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 16.889045 KDa

Sequence

String:

EEHTFETADA GSSATYPMQC SALRKNGFVV IKSRPCKIVD MSTSKTG(5CT)HG HAKVHLVAID IFTGKKLEDL SPSTHN MEV PVVKRNEYQL LDIDDGFLSL MNMDGDTKDD VKAPEGELGD SLQTAFDEGK DLMVTIISAM GEEAAISFKE AARTD

Macromolecule #85: L1 60S ribosomal protein

• Macromolecule: Name: L1 60S ribosomal protein / type: protein_or_peptide / ID: 85 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 17.889996 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #86: GCN1

• Macromolecule: Name: GCN1 / type: protein_or_peptide / ID: 86 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast)
• Molecular weight: Theoretical: 98.12518 KDa

Sequence

String:

AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA GDYLGILMEK L LNPTVASS MRKGAAWGIA GLVKGYGISA LSEFDIIRNL IEAAEDKKEP KRRESVGFCF QYLSESLGKF FEPYVIEILP NI LKNLGDA VPEVRDATAR ATKAIMAHTT GYGVKKLIPV AVSNLDEIAW RTKRGSVQLL GNMAYLDPTQ LSASVSTIVP EIV GVLNDS HKEVRKAADE SLKRFGEVIR NAAIQKLVPV LLQAIGDPTK YTEEALDSLI QTQFVHYIDG PSLALIIHII HRGM HDRSA NIKRKACKIV GNMAILVDTK DLIPYLQQLL DEVEIAMVDP VPNTRATAAR ALGALVERLA AAAAAAAAAA AAAAA AAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAA AAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAA AA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAA A AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA A AAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AA AAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAA AAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAA AAAAA AAAA

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE-PROPANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELDBright-field microscopy
• Image recording: Film or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 2.5 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 30016