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- EMDB-28465: Liver carboxylesterase 1 -

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Basic information

Entry
Database: EMDB / ID: EMD-28465
TitleLiver carboxylesterase 1
Map data
Sample
  • Complex: Liver carboxylesterase 1
    • Protein or peptide: Liver carboxylesterase 1
  • Ligand: ETHYL ACETATE
Keywordscarboxylesterase / human liver / HYDROLASE
Function / homology
Function and homology information


cholesterol ester hydrolysis involved in cholesterol transport / methylumbelliferyl-acetate deacetylase / methylumbelliferyl-acetate deacetylase activity / regulation of bile acid secretion / sterol esterase / sterol ester esterase activity / medium-chain fatty acid metabolic process / carboxylesterase / Physiological factors / carboxylesterase activity ...cholesterol ester hydrolysis involved in cholesterol transport / methylumbelliferyl-acetate deacetylase / methylumbelliferyl-acetate deacetylase activity / regulation of bile acid secretion / sterol esterase / sterol ester esterase activity / medium-chain fatty acid metabolic process / carboxylesterase / Physiological factors / carboxylesterase activity / regulation of bile acid biosynthetic process / cellular response to cholesterol / positive regulation of cholesterol metabolic process / reverse cholesterol transport / Phase I - Functionalization of compounds / carboxylic ester hydrolase activity / cholesterol biosynthetic process / Aspirin ADME / negative regulation of cholesterol storage / cellular response to low-density lipoprotein particle stimulus / positive regulation of cholesterol efflux / cholesterol metabolic process / Metabolism of Angiotensinogen to Angiotensins / lipid catabolic process / lipid droplet / epithelial cell differentiation / cholesterol homeostasis / response to toxic substance / endoplasmic reticulum lumen / endoplasmic reticulum / cytosol / cytoplasm
Similarity search - Function
: / Carboxylesterase type B, active site / Carboxylesterases type-B serine active site. / Carboxylesterase type B, conserved site / Carboxylesterases type-B signature 2. / Carboxylesterase, type B / Carboxylesterase family / Alpha/Beta hydrolase fold
Similarity search - Domain/homology
Liver carboxylesterase 1
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.67 Å
AuthorsZhang Z / Yu E
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) United States
CitationJournal: Cell Rep / Year: 2023
Title: High-resolution structural-omics of human liver enzymes.
Authors: Chih-Chia Su / Meinan Lyu / Zhemin Zhang / Masaru Miyagi / Wei Huang / Derek J Taylor / Edward W Yu /
Abstract: We applied raw human liver microsome lysate to a holey carbon grid and used cryo-electron microscopy (cryo-EM) to define its composition. From this sample we identified and simultaneously determined ...We applied raw human liver microsome lysate to a holey carbon grid and used cryo-electron microscopy (cryo-EM) to define its composition. From this sample we identified and simultaneously determined high-resolution structural information for ten unique human liver enzymes involved in diverse cellular processes. Notably, we determined the structure of the endoplasmic bifunctional protein H6PD, where the N- and C-terminal domains independently possess glucose-6-phosphate dehydrogenase and 6-phosphogluconolactonase enzymatic activity, respectively. We also obtained the structure of heterodimeric human GANAB, an ER glycoprotein quality-control machinery that contains a catalytic α subunit and a noncatalytic β subunit. In addition, we observed a decameric peroxidase, PRDX4, which directly contacts a disulfide isomerase-related protein, ERp46. Structural data suggest that several glycosylations, bound endogenous compounds, and ions associate with these human liver enzymes. These results highlight the importance of cryo-EM in facilitating the elucidation of human organ proteomics at the atomic level.
History
DepositionOct 4, 2022-
Header (metadata) releaseMay 3, 2023-
Map releaseMay 3, 2023-
UpdateNov 13, 2024-
Current statusNov 13, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_28465.png

Downloads & links

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Map

FileDownload / File: emd_28465.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 300 pix.
= 324. Å		1.08 Å/pix.
x 300 pix.
= 324. Å		1.08 Å/pix.
x 300 pix.
= 324. Å

Surface

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Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.08 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.2
Minimum - Maximum-2.1599586 - 3.0431516
Average (Standard dev.)-0.00045653922 (±0.06072187)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 324.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_28465_additional_1.map
Projections & Slices
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Half map: #2

Fileemd_28465_half_map_1.map
Projections & Slices
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Half map: #1

Fileemd_28465_half_map_2.map
Projections & Slices
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Sample components

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Entire : Liver carboxylesterase 1

EntireName: Liver carboxylesterase 1
Components
  • Complex: Liver carboxylesterase 1
    • Protein or peptide: Liver carboxylesterase 1
  • Ligand: ETHYL ACETATE

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Supramolecule #1: Liver carboxylesterase 1

SupramoleculeName: Liver carboxylesterase 1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Liver carboxylesterase 1

MacromoleculeName: Liver carboxylesterase 1 / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO / EC number: carboxylesterase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 62.587773 KDa
SequenceString: MWLRAFILAT LSASAAWGHP SSPPVVDTVH GKVLGKFVSL EGFAQPVAIF LGIPFAKPPL GPLRFTPPQP AEPWSFVKNA TSYPPMCTQ DPKAGQLLSE LFTNRKENIP LKLSEDCLYL NIYTPADLTK KNRLPVMVWI HGGGLMVGAA STYDGLALAA H ENVVVVTI ...String:
MWLRAFILAT LSASAAWGHP SSPPVVDTVH GKVLGKFVSL EGFAQPVAIF LGIPFAKPPL GPLRFTPPQP AEPWSFVKNA TSYPPMCTQ DPKAGQLLSE LFTNRKENIP LKLSEDCLYL NIYTPADLTK KNRLPVMVWI HGGGLMVGAA STYDGLALAA H ENVVVVTI QYRLGIWGFF STGDEHSRGN WGHLDQVAAL RWVQDNIASF GGNPGSVTIF GESAGGESVS VLVLSPLAKN LF HRAISES GVALTSVLVK KGDVKPLAEQ IAITAGCKTT TSAVMVHCLR QKTEEELLET TLKMKFLSLD LQGDPRESQP LLG TVIDGM LLLKTPEELQ AERNFHTVPY MVGINKQEFG WLIPMQLMSY PLSEGQLDQK TAMSLLWKSY PLVCIAKELI PEAT EKYLG GTDDTVKKKD LFLDLIADVM FGVPSVIVAR NHRDAGAPTY MYEFQYRPSF SSDMKPKTVI GDHGDELFSV FGAPF LKEG ASEEEIRLSK MVMKFWANFA RNGNPNGEGL PHWPEYNQKE GYLQIGANTQ AAQKLKDKEV AFWTNLFAKK AVEKPP QTE HIEL

UniProtKB: Liver carboxylesterase 1

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Macromolecule #3: ETHYL ACETATE

MacromoleculeName: ETHYL ACETATE / type: ligand / ID: 3 / Number of copies: 3 / Formula: EEE
Molecular weightTheoretical: 88.105 Da
Chemical component information

ChemComp-EEE:
ETHYL ACETATE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 41.25 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.291 µm / Nominal defocus min: 0.17 µm / Nominal magnification: 81000
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.67 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 210633
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION
FSC plot (resolution estimation)

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