EMDB-22090: Vigna radiata mitochondrial complex I*

Basic information

• Entry: Database: EMDB / ID: EMD-22090
• Title: Vigna radiata mitochondrial complex I*
• Map data: Composite map of Vigna radiata mitochondrial complex I* from the combination of locally refined maps of the membrane arm and peripheral arm. Composite map was generated using PHENIX.

Sample

• Complex: Vigna radiata complex I*
  • Protein or peptide: x 34 types
• Ligand: x 6 types

Function / homology

Function:

oxidoreductase activity, acting on NAD(P)H / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / electron transport chain / NADH dehydrogenase (ubiquinone) activity / quinone binding / ATP synthesis coupled electron transport / respirasome / catalytic activity / respiratory electron transport chain / 2 iron, 2 sulfur cluster binding / NAD binding / FMN binding / 4 iron, 4 sulfur cluster binding / mitochondrial inner membrane / membrane => GO:0016020 / oxidoreductase activity / mitochondrion / RNA binding / membrane / metal ion binding

Domain/homology:

NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / Hexapeptide repeat of succinyl-transferase / Soluble ligand binding domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / SLBB domain / Zinc finger, CHCC-type / Zinc-finger domain / Hexapeptide repeat / GRIM-19 / GRIM-19 protein / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / Bacterial transferase hexapeptide (six repeats) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH ubiquinone oxidoreductase, F subunit / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. / Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. / Trimeric LpxA-like superfamily / CHCH / NuoE domain / CHCH domain / NADH-quinone oxidoreductase subunit E-like / NADH-quinone oxidoreductase subunit E, N-terminal / NADH:ubiquinone oxidoreductase, subunit G, iron-sulphur binding / His(Cys)3-ligated-type [4Fe-4S] domain profile. / NADH:ubiquinone oxidoreductase, 51kDa subunit, conserved site / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2. / NADH-ubiquinone oxidoreductase 51kDa subunit, FMN-binding domain / NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain / NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain superfamily / NADH-ubiquinone oxidoreductase 51kDa subunit, FMN-binding domain superfamily / Respiratory-chain NADH dehydrogenase 51 Kd subunit / NADH-ubiquinone oxidoreductase-F iron-sulfur binding region / NADH-ubiquinone oxidoreductase-F iron-sulfur binding region / Molybdopterin oxidoreductase, 4Fe-4S domain / Prokaryotic molybdopterin oxidoreductases 4Fe-4S domain profile. / NAD-dependent epimerase/dehydratase / NAD dependent epimerase/dehydratase family / NADH:ubiquinone oxidoreductase-like, 20kDa subunit / NADH ubiquinone oxidoreductase, 20 Kd subunit / Molybdopterin oxidoreductase / Molybdopterin oxidoreductase / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / 4Fe-4S dicluster domain / 2Fe-2S ferredoxin-type iron-sulfur binding domain profile. / 2Fe-2S ferredoxin-type iron-sulfur binding domain / 2Fe-2S ferredoxin-like superfamily / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain / RNA recognition motif / RNA recognition motif / Eukaryotic RNA Recognition Motif (RRM) profile. / RNA recognition motif domain / RNA-binding domain superfamily / Thioredoxin-like superfamily / Nucleotide-binding alpha-beta plait domain superfamily / NAD(P)-binding domain superfamily

Component:

uncharacterized protein LOC106754061 / uncharacterized protein At2g27730, mitochondrial / serine/arginine-rich-splicing factor SR34 isoform X2 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial / gamma carbonic anhydrase 1, mitochondrial-like / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / gamma carbonic anhydrase-like 2, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / uncharacterized protein LOC106767534 / uncharacterized protein LOC106768957 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial isoform X1 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / uncharacterized protein LOC106775330 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / gamma carbonic anhydrase 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

• Biological species: Vigna radiata (mung bean) / mung bean (mung bean) / Mung bean (mung bean)
• Method: single particle reconstruction / cryo EM / Resolution: 3.9 Å
• Authors: Letts JA / Maldonado M / Padavannil A / Zhou L / Guo F
• Citation: Journal: Elife / Year: 2020; Title: Atomic structure of a mitochondrial complex I intermediate from vascular plants.; Authors: Maria Maldonado / Abhilash Padavannil / Long Zhou / Fei Guo / James A Letts / ; Abstract: Respiration, an essential metabolic process, provides cells with chemical energy. In eukaryotes, respiration occurs via the mitochondrial electron transport chain (mETC) composed of several large membrane-protein complexes. Complex I (CI) is the main entry point for electrons into the mETC. For plants, limited availability of mitochondrial material has curbed detailed biochemical and structural studies of their mETC. Here, we present the cryoEM structure of the known CI assembly intermediate CI* from at 3.9 Å resolution. CI* contains CI's NADH-binding and CoQ-binding modules, the proximal-pumping module and the plant-specific γ-carbonic-anhydrase domain (γCA). Our structure reveals significant differences in core and accessory subunits of the plant complex compared to yeast, mammals and bacteria, as well as the details of the γCA domain subunit composition and membrane anchoring. The structure sheds light on differences in CI assembly across lineages and suggests potential physiological roles for CI* beyond assembly.

History

Deposition	Jun 1, 2020	-
Header (metadata) release	Sep 2, 2020	-
Map release	Sep 2, 2020	-
Update	Sep 2, 2020	-
Current status	Sep 2, 2020	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_22090.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Composite map of Vigna radiata mitochondrial complex I* from the combination of locally refined maps of the membrane arm and peripheral arm. Composite map was generated using PHENIX.
• Voxel size: X=Y=Z: 0.8332 Å

Density

Contour Level	By AUTHOR: 6.5 / Movie #1: 6.5
Minimum - Maximum	-24.12702 - 59.245678
Average (Standard dev.)	-0.0004901062 (±1.0816678)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order Z Y X Origin 0 0 0 Dimensions 512 512 512 Spacing 512 512 512
Cell	A=B=C: 426.5984 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	0.83319921875	0.83319921875	0.83319921875
M x/y/z	512	512	512
origin x/y/z	0.000	0.000	0.000
length x/y/z	426.598	426.598	426.598
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	512	512	512
MAP C/R/S	3	2	1
start NC/NR/NS	0	0	0
NC/NR/NS	512	512	512
D min/max/mean	-24.127	59.246	-0.000

Supplemental data

Sample components

Entire : Vigna radiata complex I*

• Entire: Name: Vigna radiata complex I*

Components

• Complex: Vigna radiata complex I*
  • Protein or peptide: Unknown Peptide
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
  • Protein or peptide: NDUA7
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial isoform X1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial
  • Protein or peptide: NDUS2
  • Protein or peptide: NDUS3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
  • Protein or peptide: NU1M
  • Protein or peptide: NU2M
  • Protein or peptide: NU3M
  • Protein or peptide: NU4L
  • Protein or peptide: NU6M
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
  • Protein or peptide: uncharacterized protein LOC106754061
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A
  • Protein or peptide: Unknown Peptide
  • Protein or peptide: Unknown Peptide
  • Protein or peptide: NDUC2
  • Protein or peptide: Protein At2g27730, mitochondrial
  • Protein or peptide: serine/arginine-rich-splicing factor SR34 isoform X2
  • Protein or peptide: NDUX1
  • Protein or peptide: gamma carbonic anhydrase 1, mitochondrial
  • Protein or peptide: gamma carbonic anhydrase 1, mitochondrial-like
  • Protein or peptide: gamma carbonic anhydrase-like 2, mitochondrial
• Ligand: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
• Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
• Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
• Ligand: FE2/S2 (INORGANIC) CLUSTER
• Ligand: ZINC ION
• Ligand: FLAVIN MONONUCLEOTIDE

Supramolecule #1: Vigna radiata complex I*

• Supramolecule: Name: Vigna radiata complex I* / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#34; Details: Complex I 800 kDa assembly intermediate (complex I*)
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 800 KDa

Macromolecule #1: Unknown Peptide

• Macromolecule: Name: Unknown Peptide / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: mung bean (mung bean)
• Molecular weight: Theoretical: 1.549902 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #2: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 10.884632 KDa

Sequence

String:

MAWRGQLSKN IKELRFLMCQ SSSASASARA FVEKNYKELK TLNPKLPILI RECSGVEPQL WARYDLGVEK AIKLEGLSEA QISKALEDL AKAGQSSKA

Macromolecule #3: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial; type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 18.977604 KDa

Sequence

String:

MFLRAVARPL MAKVKASTGI VGLEVVPNAR EVLIGLYSKT LKEIQKVPED EGYRKAVESF TKHRLSVCKE EEDWEAIEKR LGCGQVEEL IEEAQDELKL ISYMIEWDPW GVPDDYECEV IENDAPVPKH VPLHRPPPLP TEFHKTLEAL SSQSGKDIPG A SSTESPSK T

Macromolecule #4: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6; type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 14.920034 KDa

Sequence

String:

MASALRNVKV LPNSASVEEA RQRVFDFFRA TCRSLPSVME IYNLYDVTSV AQLRSTVASE IRKNIGVSDP KVIDMLLFKG MEELMNVVN HSKQRHHIIG QYVVGQQGQV QDSASKDPAI SPFLKNFYSS NYF

Macromolecule #5: NDUA7

• Macromolecule: Name: NDUA7 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 14.356376 KDa

Sequence

String:

MAKSASNSLV QTLKRYIKKP WEITGPCADP EYRSAVPLAT EYRLQCPATT KEKPCIPNSL PETVYDIKYF SRDQRRNRPP IRRTVLKKA DVEKLAKEQT FAVSDFPPVY LNSAVEEDIN AIGGGYQG

Macromolecule #6: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial isoform X1; type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 43.781504 KDa

Sequence

String:

MQTIARRLGH QSLKSSTSIR AIYPISDHHY VADHQRYVST IATKGVGHLV RKGTGGRSSV SGIIATVFGA TGFLGRYVVQ QLAKMGSQV LVPFRGSEDC PRHLKLMGDL GQIVPMKYNP RDESSVKAVM AKANVVINLI GRDYETRNYS FEEVHYHMAE N LAKISREH GGILRFIQVS CLGASPSSPS RMLRAKAAAE EVVLRELPEA TILKPAVMIG TEDRILNPWA HFAKKYGFIP LF GNGSTKI QPVYVVDVAA ALTTVLKDDG TSMGKTYELG GPEIFTVHDL AELMYETIRE WPRYVKVPFP IAKALATPRE ILL NKVPFP LPTPEILNLD KIQALTTDTI VSENALTFND LGIIPHKLKG YPVEFLISYR KGGPQFGSTI SERVTPDAWP

Macromolecule #7: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12; type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 18.192482 KDa

Sequence

String:

MASVVKNVLK SIREKGFGTF LRELKDEGYL RCLPDGNLLQ TKIHNIGATL VGVDKFGNKY YEKLGDTQYG RHRWVEYAEK SRYNASQVP PEWHGWLHFI TDHTGDELLL LKPKRYGVEH KENLSGEGEQ YIYHSKGHAL NPGQRDWTRY QPWESKA

Macromolecule #8: NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial; type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 81.434219 KDa

Sequence

String:

MGFGLLASKA LRPTSRLLLS SQNPTIFLRT IVSKPQLCNP EASAAQPAQP PPVDLPPRSP LAGARVHFAN PDDAIEVFVD GYPVKIPKG MTVLQACEVA GVDIPRFCYH SRLSIAGNCR MCLVEVEKSP KPVASCAMPA LPGMKIKTDT PVAKKAREGV M EFLLMNHP LDCPICDQGG ECDLQDQSMA FGSDRGRFTE VKRSVVDKNL GPLVKTVMTR CIQCTRCVRF ATEVAGVQDL GM LGRGSGE EIGTYVEKLL TSELSGNVID ICPVGALTSK PFAFKARNWE LKGTETIDVT DAVGSNIRID SRGPEVMRIV PRL NEDINE EWISDKTRFC YDGLKRQRLN DPMIRGPDGR FKAVNWRDAL SVIADIAHQV KPEEIVGVAG KLSDAESMIA LKDF LNRMG SNDVWGEGIG VNTNADFRSG YIMNTSIAGL EKADVFLLVG TQPRVEAAMV NARIRKTVRS NQAKVGYIGP ATDFN YDHK HLGTDPQTLV EIAEGRHPFF KTLSDAKNPV IIVGAGVFER KDQDAIFAAV ETIAQKANVV RPDWNGLNVL LLHAAQ AAA LDLGLVPQSE KSLESAKFVY LMGADDVNLD KIPDDAFVVY QGHHGDKSVY RANVILPTAA FSEKEGTYQN TEGCTQQ TL PAVPTVGDSR DDWKIIRALS EVAGVRLPYD TIGAVRARIR NVAPNLVNVD EREPATLPSS LRPSFTQKVD TTPFGTVI E NFYMTDAITR ASKIMAQCSA TLLKK

Macromolecule #9: NDUS2

• Macromolecule: Name: NDUS2 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: mung bean (mung bean)
• Molecular weight: Theoretical: 44.813281 KDa

Sequence

String:

MTTRNGQIKN FTSNFGPQHP AAHGVLRSVL EMNGEVVERA EPHIGLLHRG TEKLIEYKTY LQALPYFDRL DYVSTMAQEH AYSLAVERL LNCEVPLRAQ YIRVLFCEIT RILNHLLALT THAMDVGAST PSLWAFEERE KLLEFYERVS GARMHASFIR P GGVAQDLP LGLCRDIDSF TQQFASRIDE LEEMLTGNRI WKQRLVDIGT VTAQQAKDWG FSGVMLRGPG VCWDLRRAAP YD VYDQLDF DVPVGTRGDC YDRYCIRIEE MRQSMRIIWQ CPNKIPSGMI KADDRKLCPP SRCRMKLSME SSIHHFELYT EGF SVPAPS TYTAVEAPKG EFGVFLVSNG SNRPYRCKIR APGFAHLQGL DFMSKHHMLA DVVTIIGTQD IVSGEVDR

Macromolecule #10: NDUS3

• Macromolecule: Name: NDUS3 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: mung bean (mung bean)
• Molecular weight: Theoretical: 22.806779 KDa

Sequence

String:

MDNQSIFKYS WETLPKKWVK KMERSEHGNR FDTKTDYLFQ LLCFLKLHTY TRVQVLIDIC GVDYPSRKRR FEVVYNLLST RYNSRIRVQ TSADEVTRIS SVVSLFPSAG WWEREVWDMF GVSSINHPDL RRILTDYGFE GHPLRKDFPL SGYVEVRYDD P EKRVVSEP IEMTQEFRYF DFASPWEQRS DG

Macromolecule #11: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 16.435736 KDa

Sequence

String:

MANTLQRALR ATMARRFSTQ ALVETKPGEI GMVSGIPQEH LRRRVVIYSP ARTASQQGSG KVGRWKINFL STQKWENPLM GWTSTGDPY AHVGDSALTF DSAEAAKAFA EKHGWEYSVK KRHTPLLKVK SYADNFKWKG FPKPAEE

Macromolecule #12: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 11.582296 KDa

Sequence

String:

MASSVLRNLM RFSSPRNTST RSFSLVTSQI SNHTAKWMQD TSKKSPMELI NEVPPIKVEG RIVACEGDTN PALGHPIEFI CLDLPEPAV CKYCGLRYVQ DHHH

Macromolecule #13: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 23.710473 KDa

Sequence

String:

MALLSRASSR IYQVHSYQRA LSLHTTLPAL NASTSTHTPT PYAPPPPPSA SSPVGVSKAA EFVISKVDDL MNWARRGSIW PMTFGLACC AVEMMHTGAA RYDLDRFGII FRPSPRQSDC MIVAGTLTNK MAPALRKVYD QMPEPRWVIS MGSCANGGGY Y HYSYSVVR GCDRIVPVDI YVPGCPPTAE ALLYGLLQLQ KKINRRKDFL HWWTK

Macromolecule #14: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 25.54501 KDa

Sequence

String:

MAAILARKSL LALRARQLAV SGQGLHSSQS YGLRLSAHSF STKLEDEQRE QLAKEISKDW SSVFERSINT LFLTEMVRGL MLTLKYFFE TKVTINYPFE KGPLSPRFRG EHALRRYPTG EERCIACKLC EAICPAQAIT IEAEEREDGS RRTTRYDIDM T KCIYCGFC QEACPVDAIV EGPNFEFATE THEELLYDKE KLLENGDRWE TEIAENLRSE SLYR

Macromolecule #15: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH dehydrogenase
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 53.817582 KDa

Sequence

String:

MRGILSLRSA LVQHRSEKLG LGLRQFSTQG ASTPTSPQPP PPPASPQPPP PPPPPEKTHF GGLKDEDRIF TNLYGLHDPF LKGAMKRGD WYRTKDLVIK GTDWIVNEMK KSGLRGRGGA GFPSGLKWSF MPKVSDGRPS YLVVNADESE PGTCKDREIM R HDPHKLLE GCLIAGVGMR ATAAYIYIRG EYVNERKNLE KARQEAYAAG LLGKNACGSG YDFDVHIHFG AGAYICGEET AL LESLEGK QGKPRLKPPF PANAGLYGCP TTVTNVETVA VSPTILRRGP EWFAGFGRKN NAGTKLFCVS GHVNKPCTVE EEM SIPLKE LIERHCGGVR GGWDNLLAVI PGGSSVPLLP KSICDDVLMD YDALKAVQSG LGTAAVIVMD KSTDVVDAIA RLSY FYKHE SCGQCTPCRE GTGWLWTIME RMKVGNAKLE EIDMLQELTK QIEGHTICAL GDAAAWPVQG LIRHFRPELE RRIRE NAER ELLQATG

Macromolecule #16: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 27.992949 KDa

Sequence

String:

MLARLAANRF NEIRHIFRQP SRAFSTALNY HLDSPDNNPN LPWEFTEPNK AKVTEILSHY PSNYKQSAVI PLLDLAQQQH GGWLPVSAM NAVANIIEVP PIRVYEVATF YSMFNRAKVG KYHLLVCGTT PCMIRGSRGI EEALLKHLGV KRNEVTPDGL F SVGEMECM GCCVNAPMIT VADYSNGSEG YTYNYYEDVT PEKVVEIVEK LRKGEKPPHG TQNPLRIRSG PEGGNTTLLG EP KPPPCRD LDAC

Macromolecule #17: NU1M

• Macromolecule: Name: NU1M / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 35.651367 KDa

Sequence

String:

MYIAVPAEIL GIILPLLLGV AFLVLAERKV MAFVQRRKGP DVVGSFGLLQ PLADGLKLIL KEPISPSSAN FSLFRMAPVA TFMLSLVAW AVVPFDYGMV LSDSNIGLLY LFAISSLGVY GIITAGWSSN SKYAFLGALR SAAQMVSYEV SIGLILITVL I CVGSCNLS EIVMAQKQIW FGIPLFPVLV MFFISCLAET NRAPFDLPEA EAESVAGYNV EYSSMGSALF FLGEYANMIL MS GLCTSLS LGGWLPILDL PIFKRIPGSI WFSIKVILFL FLYIWVRAAF PRYRYDQLMG LGWKVFLPLS LAWVVAVSGV SVT FPWLP

Macromolecule #18: NU2M

• Macromolecule: Name: NU2M / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 53.900664 KDa

Sequence

String:

MFNLFLAVYP EIFIINATFI LLIHGVVFST SKKYDYPPLV SNVGWLGLLS VLITLLLLAA GAPLLTIAHL FRNNLFRRDN FTYFCQILL LLSTAGTISM CFDFFEQERF DAFEFIVLIL LSTCSMLFMI SAYDSIAMYL AIELQSLCFY VMAASKRKSE F STEAGLKY LILGAFSSGI LLFGCSMIYG STGATHFDQL AKILTGYEIT GARSSGIFMG ILFIAVGSLF KITAVPFHMW AP DIYEGSP TPVTAFLSIA PKISIFANIS RVSIYGSYGA TLQQIFCFCS IASMILGALA AMAQTKVKRL LAYSSIGHVG YIC TGFSCG TIEGIQSLLI GIFIYALMTI DAFAIVLALR QTRVKYIADL GALAKTNPIL AITFSITMFS YVGIPPLAGF CSKF YLFFA ALGCGAYFLA PVGVVTSVIG CFYYIRLAKR MFFDTPRTWI LYEPMDRDKS LLLAMTSSFI TLFFPYPSPL FSVTH QMAL SLYL

Macromolecule #19: NU3M

• Macromolecule: Name: NU3M / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 13.507715 KDa

Sequence

String:

MSEFAPICIY LVISLLVSLI LLGLPFLFAS NSSTYPEKLS AYECGFDPFG DARSRFDIRF YLVSILFIIF DPEVTFSFPW AVSLNKIDP FGSWSMMAFL LILTIGSLYE WKRGALDWE

Macromolecule #20: NU4L

• Macromolecule: Name: NU4L / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 11.082356 KDa

Sequence

String:

MDPIKYFTFS MIIFILGIRG ILLNRRNILI MLMSIELMLL AVNSNFLVFS VSLDDMMGQS FALLVLTVAA AESAIGLAIF VITFRVRGT IAVEFINSIQ G

Macromolecule #21: NU6M

• Macromolecule: Name: NU6M / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 23.484178 KDa

Sequence

String:

MILSVLSSLA LVSGLMVVRA KNPVHSVLFF ILVFCDTSGL LLLLGLDFSA MIFLVVYIGA IAVLFLFVVM MFHIQIAEIH EEVLRYLPV SGIIGLILWW EMFFILDNET IPLLPTQINT TSLIYTVYAG KVRSWTNLET LGNLLYTYYF VWFLVPSLIL L VAMIGAIV LTMHRTTKVK RQDVFRRNAI DFRRTIMRRT TDPLTID

Macromolecule #22: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 7.357572 KDa

Sequence

String:

MNLRWMEAVL PLGIIAGMLC VMGNAQYYIH KAAHGRPKHI GNDLWDVAME RRDKKLHEQA ASSSN

Macromolecule #23: uncharacterized protein LOC106754061

• Macromolecule: Name: uncharacterized protein LOC106754061 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 6.695044 KDa

Sequence

String:

MAVMEKLKMF VVQEPVVAAS CLIAGFGLFL PAVVRPILDS YQATKQPPQP ALSDVVAGMT GKK

Macromolecule #24: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B; type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 12.022893 KDa

Sequence

String:

MASAVDASGN PIPSSSVLMA SSKHIGIRCH SENLDFLKCK KKDPNPEKCL DKGRDVTRCV LGLLKDLHQK CTKEMDDYVG CMYYHTNEF DLCRKEQQAF EKKCSLE

Macromolecule #25: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A; type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 16.1896 KDa

Sequence

String:

MTEAYIRNKP GMVSVKDMPV LQDGPPPGGF APVRFARRIP NKGPSAVAIF LAAFGTFSWG MYQVGQGNKI RRALKEEKYA ARRSILPVL QAEEDERFVR EWHKYLAYEA EVMKDVPGWK VGESVYNSGR WVPPASGELR PDIW

Macromolecule #26: Unknown Peptide

• Macromolecule: Name: Unknown Peptide / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 2.145636 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #27: Unknown Peptide

• Macromolecule: Name: Unknown Peptide / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 3.677524 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #28: NDUC2

• Macromolecule: Name: NDUC2 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 9.130512 KDa

Sequence

String:

MVLSATTIGA LLGLGTQMYS NALRKLPYMR HPWEHVVGMG LGAVFVNQLL KWEAQVEQDL DKMLEKAKAA NERRYIDGDD D

Macromolecule #29: Protein At2g27730, mitochondrial

• Macromolecule: Name: Protein At2g27730, mitochondrial / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 12.172666 KDa

Sequence

String:

MAARVAARYG SRRLFSSGSG KILSEEEKAA ENAYFKKAEQ DKLEKLARKG PQPEASSGGS VIDAKPSGSG HTGASAERVS TDKHRNYAV VAGTITILGA LGWYLKGTAK KPEVQD

Macromolecule #30: serine/arginine-rich-splicing factor SR34 isoform X2

• Macromolecule: Name: serine/arginine-rich-splicing factor SR34 isoform X2 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 45.640945 KDa

Sequence

String:

MASGWGITGN KGRCYDFWMD FSECMSRCRE PKDCGLLRED YLECLHHSKE FQRRNRIYKE EQRKIRAAAK KAKDDGVVKE HHYLHGNTT DSNPHLSKVK KMSRRSSRTV YVGNLPGDIR EREVEDLFLK YGHITHIDLK VPPRPPGYAF VEFEDAQDAE D AIRGRDGY DFDGHRLRVE PAHGGRGHSS SRDRHSSHSN GRAGRGVSRR SEYRVLVTGL PSSASWQDLK DHMRKAGDVC FS QVFHDGR GTTGIVDYTN YDDMKYAIKK LDDSEFRNAF SKGYVRVREY DSRRDSSRSP SRGPSHSRGR SYSRSRSRSR SYS RDRSQS KSPKGKSSQR SPPAKSPSRS PAARSRSRSR SRSLSGSRSR SRSPLPPRNK SPKRGSASRS PSRSRSRSKS LSR

Macromolecule #31: NDUX1

• Macromolecule: Name: NDUX1 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 10.94954 KDa

Sequence

String:

MNTDITASTK PEYPVVDRNP PFTKVVGNFS TLDYLRFVTI TGVSVTVGYL SGIKPGIRGP SMVTGGLIGV MGGFMYAYQN SAGRLMGFF PNDDEVARHN KK

Macromolecule #32: gamma carbonic anhydrase 1, mitochondrial

• Macromolecule: Name: gamma carbonic anhydrase 1, mitochondrial / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 29.333197 KDa

Sequence

String:

MGTLGRAFYA VGFWIRETGQ AIDRLGSRLQ GNYLFQEQLS RHRPLMNLFD KYPSVHKDAF VAPSASLLGD VHVGPASSIW YGCVLRGDV NGIVIGSGTN IQDNSLVHVA KSNLTGKVLP TIIGDNVTVG HSAVLQGCTV EDEAFIGMGA TLLDGVYVEK H AMVAAGAL VRQNTRIPYG EVWGGNPAKF LRKLTEDEMA FFSQSALNYS NLAQAHAAEN AKELEETEFV KVLHKKFAPR GE EYDSILD GGQETPAKLN LQDNVLLDKA PKA

Macromolecule #33: gamma carbonic anhydrase 1, mitochondrial-like

• Macromolecule: Name: gamma carbonic anhydrase 1, mitochondrial-like / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 29.780861 KDa

Sequence

String:

MGTLGRAIYS VGFWIRETGQ AIDRLGSRLQ GGYFFQEQLS RHRTLMNIFD KAPVVDKDVF VAPSASVIGD VQVGRGSSIW YGCVLRGDV NSIRVGSGTN IQDNSLVHVA KSNLSGKVLP TVIGDNVTVG HSAVLHGCTV EDEAFVGMGA VLLDGVVVEK N AMVAAGAL VRQNTRIPSG EVWAGNPAKF LRKLSNEEIT FISQSAINYT NLAQVHAAEN SKSYDEIEFE KVLRKKYARK DE EYDSMLG VVREIPPELI LPDNVLPDKA EKALQK

Macromolecule #34: gamma carbonic anhydrase-like 2, mitochondrial

• Macromolecule: Name: gamma carbonic anhydrase-like 2, mitochondrial / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mung bean (mung bean)
• Molecular weight: Theoretical: 28.268359 KDa

Sequence

String:

MANLARFSKR ALRSAHSLVR HAQSQVQPQL FAEERAFSTE AAKSITPSPD RVKWDYRGQR KIIPLGQWLP KVAVDAYVAP NVVLAGQVT VWDGASVWPG CVLRGDLNKI SIGFCSNVQE RSVLHAAWTS PTGLPADTSI ERYVTIGAYS LLRSCTIEPE C IIGQHSIL MEGSLVETQS ILEAGSVVPP GRRIPSGELW AGNPARFVRT LTHEEILEIP KLAVAINDLS RDHYSEFLPY ST VYLEVEK FKKSLGISV

Macromolecule #35: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

• Macromolecule: Name: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / type: ligand / ID: 35 / Number of copies: 1 / Formula: NAP
• Molecular weight: Theoretical: 743.405 Da

Chemical component information

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

Macromolecule #36: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

• Macromolecule: Name: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 36 / Number of copies: 6 / Formula: PC1
• Molecular weight: Theoretical: 790.145 Da

Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM / Phosphatidylcholine

Macromolecule #37: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 37 / Number of copies: 6 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

Macromolecule #38: FE2/S2 (INORGANIC) CLUSTER

• Macromolecule: Name: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 38 / Number of copies: 2 / Formula: FES
• Molecular weight: Theoretical: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

Macromolecule #39: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 39 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #40: FLAVIN MONONUCLEOTIDE

• Macromolecule: Name: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 40 / Number of copies: 1 / Formula: FMN
• Molecular weight: Theoretical: 456.344 Da

Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 6 mg/mL

Buffer

pH: 7.8
Component:

Concentration	Formula	Name
20.0 mM	HEPES	HEPES
150.0 mM	NaClSodium chloride	sodium chloride
0.1 %	digitonin	digitonin
1.0 mM	edtaEthylenediaminetetraacetic acid	EDTAEthylenediaminetetraacetic acid

• Grid: Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Details: 30 mA
• Vitrification: Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK III; Details: Blotted for 9 seconds at 100% humidity, 15 degrees C..
• Details: Extracted from V. radiata mitochondrial membranes with digitonin, exchanged into amphipole A8-35, purified on sucrose gradient.

Electron microscopy

• Microscope: TFS KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 9816 / Average exposure time: 3.0 sec. / Average electron dose: 86.4 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 190951
• CTF correction: Software - Name: CTFFIND
• Startup model: Type of model: INSILICO MODEL / In silico model: Ab initio
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION
• Final 3D classification: Number classes: 3 / Software - Name: RELION
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION
• Final reconstruction: Number classes used: 1 / Applied symmetry - Point group: C₁ (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 34407

Atomic model buiding 1

• Refinement: Space: REAL / Protocol: OTHER

Output model

PDB-6x89:
Vigna radiata mitochondrial complex I*