+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-22093 | |||||||||
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Title | Vigna radiata complex I* peripheral arm | |||||||||
Map data | Vigna radiata complex I* peripheral arm. | |||||||||
Sample |
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Biological species | Vigna radiata (mung bean) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.8 Å | |||||||||
Authors | Letts JA / Maldonado M / Padavannil A / Long Z / Guo F | |||||||||
Citation | Journal: Elife / Year: 2020 Title: Atomic structure of a mitochondrial complex I intermediate from vascular plants. Authors: Maria Maldonado / Abhilash Padavannil / Long Zhou / Fei Guo / James A Letts / Abstract: Respiration, an essential metabolic process, provides cells with chemical energy. In eukaryotes, respiration occurs via the mitochondrial electron transport chain (mETC) composed of several large ...Respiration, an essential metabolic process, provides cells with chemical energy. In eukaryotes, respiration occurs via the mitochondrial electron transport chain (mETC) composed of several large membrane-protein complexes. Complex I (CI) is the main entry point for electrons into the mETC. For plants, limited availability of mitochondrial material has curbed detailed biochemical and structural studies of their mETC. Here, we present the cryoEM structure of the known CI assembly intermediate CI* from at 3.9 Å resolution. CI* contains CI's NADH-binding and CoQ-binding modules, the proximal-pumping module and the plant-specific γ-carbonic-anhydrase domain (γCA). Our structure reveals significant differences in core and accessory subunits of the plant complex compared to yeast, mammals and bacteria, as well as the details of the γCA domain subunit composition and membrane anchoring. The structure sheds light on differences in CI assembly across lineages and suggests potential physiological roles for CI* beyond assembly. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_22093.map.gz | 480.4 MB | EMDB map data format | |
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Header (meta data) | emd-22093-v30.xml emd-22093.xml | 19.8 KB 19.8 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_22093_fsc.xml | 18.2 KB | Display | FSC data file |
Images | emd_22093.png | 63.2 KB | ||
Masks | emd_22093_msk_1.map | 512 MB | Mask map | |
Others | emd_22093_half_map_1.map.gz emd_22093_half_map_2.map.gz | 412.3 MB 411.6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-22093 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-22093 | HTTPS FTP |
-Related structure data
Related structure data | 6x89C C: citing same article (ref.) |
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Similar structure data | |
EM raw data | EMPIAR-10586 (Title: Cryo electron micrographs of digitonin-solubilized, amphipol-stabilized, sucrose-gradient-purified V. radiata mitochondrial membranes - mixed fraction containing CI*, CIII2 and SC III2+IV Data size: 6.7 TB Data #1: Raw movies of mixed sample used for determination of mung bean respiratory CI*, CIII2, CIV and SC III2+IV [micrographs - multiframe]) |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_22093.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Vigna radiata complex I* peripheral arm. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.8332 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | emd_22093_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: Vigna radiata complex I* peripheral arm half-map.
File | emd_22093_half_map_1.map | ||||||||||||
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Annotation | Vigna radiata complex I* peripheral arm half-map. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Vigna radiata complex I* peripheral arm half-map.
File | emd_22093_half_map_2.map | ||||||||||||
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Annotation | Vigna radiata complex I* peripheral arm half-map. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Vigna radiata complex I* peripheral arm
Entire | Name: Vigna radiata complex I* peripheral arm |
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Components |
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-Supramolecule #1: Vigna radiata complex I* peripheral arm
Supramolecule | Name: Vigna radiata complex I* peripheral arm / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all Details: Peripheral arm of complex I 800 kDa assembly intermediate (complex I*) |
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Source (natural) | Organism: Vigna radiata (mung bean) |
-Macromolecule #1: Vigna radiata complex I* peripheral arm
Macromolecule | Name: Vigna radiata complex I* peripheral arm / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Vigna radiata (mung bean) |
Sequence | String: MGFGLLASK ALRPTSRLLL SSQNPTIFLR TIVSKPQLCN PEASAAQPAQ PPPVDLPPRS P LAGARVHF ANPDDAIEVF VDGYPVKIPK GMTVLQACEV AGVDIPRFCY HSRLSIAGNC RM CLVEVEK SPKPVASCAM PALPGMKIKT DTPVAKKARE GVMEFLLMNH ...String: MGFGLLASK ALRPTSRLLL SSQNPTIFLR TIVSKPQLCN PEASAAQPAQ PPPVDLPPRS P LAGARVHF ANPDDAIEVF VDGYPVKIPK GMTVLQACEV AGVDIPRFCY HSRLSIAGNC RM CLVEVEK SPKPVASCAM PALPGMKIKT DTPVAKKARE GVMEFLLMNH PLDCPICDQG GEC DLQDQS MAFGSDRGRF TEVKRSVVDK NLGPLVKTVM TRCIQCTRCV RFATEVAGVQ DLGM LGRGS GEEIGTYVEK LLTSELSGNV IDICPVGALT SKPFAFKARN WELKGTETID VTDAV GSNI RIDSRGPEVM RIVPRLNEDI NEEWISDKTR FCYDGLKRQR LNDPMIRGPD GRFKAV NWR DALSVIADIA HQVKPEEIVG VAGKLSDAES MIALKDFLNR MGSNDVWGEG IGVNTNA DF RSGYIMNTSI AGLEKADVFL LVGTQPRVEA AMVNARIRKT VRSNQAKVGY IGPATDFN Y DHKHLGTDPQ TLVEIAEGRH PFFKTLSDAK NPVIIVGAGV FERKDQDAIF AAVETIAQK ANVVRPDWNG LNVLLLHAAQ AAALDLGLVP QSEKSLESAK FVYLMGADDV NLDKIPDDAF VVYQGHHGD KSVYRANVIL PTAAFSEKEG TYQNTEGCTQ QTLPAVPTVG DSRDDWKIIR A LSEVAGVR LPYDTIGAVR ARIRNVAPNL VNVDEREPAT LPSSLRPSFT QKVDTTPFGT VI ENFYMTD AITRASKIMA QCSATLLKK MTTRNGQIKN FTSNFGPQHP AAHGVSRSVL EMNGEVVERA EPHIGSLHRG TEKLIEYKTY LQALPYSDR LDYVSTMAQE HAHSSAVERL LNCEVPLRAQ YIRVLFREIT RISNHLLALT T HAMDVGAS TPSLWAFEER EKLLEFYERV SGARMHASFI RPGGVAQDLP LGLCRDIDSF TQ QFASRID ELEEMSTGNR IWKQRLVDIG TVTAQQAKDW GFSGVMLRGP GVCWDSRRAA PYD VHDQSD PDVPVGTRGD RYDRYCIRIE EMRQSMRIIW QCPNKIPSGM IKADDRKLCP PSRC RMKLS MESSIHHFEL YTEGFSVPAP STYTAVEAPK GEFGVFLVSN GSNRPYRRKI RAPGS AHSQ GLDSMSKHHM PADVVTIIGT QDIVSGEVDR MDNQSIFKY SWETLPKKWV KKMERSEHGN RSDTKTDYLF QLLCFLKLHT YTRVQVSIDI C GVDYPSRK RRFEVVYNLL STRYNSRIRV QTSADEVTRI SPVVSLFPSA GRWEREVWDM FG VSSINHP DLRRISTDYG FEGHPLRKDL PLSGYVEVRY DDPEKRVVSE PIEMTQEFRY FDF ASPWEQ RSDG MALLS RASSRIYQVH SYQRALSLHT TLPALNASTS THTPTPYAPP PPPSASSPVG VSKAA EFVI SKVDDLMNWA RRGSIWPMTF GLACCAVEMM HTGAARYDLD RFGIIFRPSP RQSDCM IVA GTLTNKMAPA LRKVYDQMPE PRWVISMGSC ANGGGYYHYS YSVVRGCDRI VPVDIYV PG CPPTAEALLY GLLQLQKKIN RRKDFLHWWT K MAAILARK SLLALRARQL AVSGQGLHSS QSYGLRLSAH SFSTKLEDEQ REQLAKEISK DW SSVFERS INTLFLTEMV RGLMLTLKYF FETKVTINYP FEKGPLSPRF RGEHALRRYP TGE ERCIAC KLCEAICPAQ AITIEAEERE DGSRRTTRYD IDMTKCIYCG FCQEACPVDA IVEG PNFEF ATETHEELLY DKEKLLENGD RWETEIAENL RSESLYR MR GILSLRSALV QHRSEKLGLG LRQFSTQGAS TPTSPQPPPP PASPQPPPPP PPPEKTHF G GLKDEDRIFT NLYGLHDPFL KGAMKRGDWY RTKDLVIKGT DWIVNEMKKS GLRGRGGAG FPSGLKWSFM PKVSDGRPSY LVVNADESEP GTCKDREIMR HDPHKLLEGC LIAGVGMRAT AAYIYIRGE YVNERKNLEK ARQEAYAAGL LGKNACGSGY DFDVHIHFGA GAYICGEETA L LESLEGKQ GKPRLKPPFP ANAGLYGCPT TVTNVETVAV SPTILRRGPE WFAGFGRKNN AG TKLFCVS GHVNKPCTVE EEMSIPLKEL IERHCGGVRG GWDNLLAVIP GGSSVPLLPK SIC DDVLMD YDALKAVQSG LGTAAVIVMD KSTDVVDAIA RLSYFYKHES CGQCTPCREG TGWL WTIME RMKVGNAKLE EIDMLQELTK QIEGHTICAL GDAAAWPVQG LIRHFRPELE RRIRE NAER ELLQATG ML ARLAANRFNE IRHIFRQPSR AFSTALNYHL DSPDNNPNLP WEFTEPNKAK VTEILSHY P SNYKQSAVIP LLDLAQQQHG GWLPVSAMNA VANIIEVPPI RVYEVATFYS MFNRAKVGK YHLLVCGTTP CMIRGSRGIE EALLKHLGVK RNEVTPDGLF SVGEMECMGC CVNAPMITVA DYSNGSEGY TYNYYEDVTP EKVVEIVEKL RKGEKPPHGT QNPLRIRSGP EGGNTTLLGE P KPPPCRDL DAC MAWRGQ LSKNIKELRF LMCQSSSASA SARAFVEKNY KELKTLNPKL PILIRECSGV EPQL WARYD LGVEKAIKLE GLSEAQISKA LEDLAKAGQS SKA MFLRAV ARPLMAKVKA STGIVGLEVV PNAREVLIGL YSKTLKEIQK VPEDEGYRKA VESF TKHRL SVCKEEEDWE AIEKRLGCGQ VEELIEEAQD ELKLISYMIE WDPWGVPDDY ECEVI ENDA PVPKHVPLHR PPPLPTEFHK TLEALSSQSG KDIPGASSTE SPSKT MASA LRNVKVLPNS ASVEEARQRV FDFFRATCRS LPSVMEIYNL YDVTSVAQLR STVASE IRK NIGVSDPKVI DMLLFKGMEE LMNVVNHSKQ RHHIIGQYVV GQQGQVQDSA SKDPAIS PF LKNFYSSNYF MAKSASNSL VQTLKRYIKK PWEITGPCAD PEYRSAVPLA TEYRLQCPAT TKEKPCIPNS L PETVYDIK YFSRDQRRNR PPIRRTVLKK ADVEKLAKEQ TFAVSDFPPV YLNSAVEEDI NA IGGGYQG MQTIARRLG HQSLKSSTSI RAIYPISDHH YVADHQRYVS TIATKGVGHL VRKGTGGRSS V SGIIATVF GATGFLGRYV VQQLAKMGSQ VLVPFRGSED CPRHLKLMGD LGQIVPMKYN PR DESSVKA VMAKANVVIN LIGRDYETRN YSFEEVHYHM AENLAKISRE HGGILRFIQV SCL GASPSS PSRMLRAKAA AEEVVLRELP EATILKPAVM IGTEDRILNP WAHFAKKYGF IPLF GNGST KIQPVYVVDV AAALTTVLKD DGTSMGKTYE LGGPEIFTVH DLAELMYETI REWPR YVKV PFPIAKALAT PREILLNKVP FPLPTPEILN LDKIQALTTD TIVSENALTF NDLGII PHK LKGYPVEFLI SYRKGGPQFG STISERVTPD AWP MASVVK NVLKSIREKG FGTFLRELKD EGYLRCLPDG NLLQTKIHNI GATLVGVDKF GNKY YEKLG DTQYGRHRWV EYAEKSRYNA SQVPPEWHGW LHFITDHTGD ELLLLKPKRY GVEHK ENLS GEGEQYIYHS KGHALNPGQR DWTRYQPWES KA MANTLQR ALRATMARRF STQALVETKP GEIGMVSGIP QEHLRRRVVI YSPARTASQQ GSG KVGRWK INFLSTQKWE NPLMGWTSTG DPYAHVGDSA LTFDSAEAAK AFAEKHGWEY SVKK RHTPL LKVKSYADNF KWKGFPKPAE E MASSVLRN LMRFSSPRNT STRSFSLVTS QISNHTAKWM QDTSKKSPME LINEVPPIKV EG RIVACEG DTNPALGHPI EFICLDLPEP AVCKYCGLRY VQDHHH |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 6 mg/mL | |||||||||||||||
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Buffer | pH: 7.8 Component:
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY | |||||||||||||||
Vitrification | Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK III Details: Blotted for 9 seconds at 100% humidity, 15 degrees C.. | |||||||||||||||
Details | Extracted from V. radiata mitochondrial membranes with digitonin, exchanged into amphipole A8-35, purified on sucrose gradient. |
-Electron microscopy
Microscope | TFS KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 9816 / Average exposure time: 3.0 sec. / Average electron dose: 86.4 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
-Atomic model buiding 1
Refinement | Space: REAL / Protocol: OTHER |
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