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基本情報
登録情報 | データベース: EMDB / ID: EMD-21145 | |||||||||
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タイトル | Structure of the bovine BBSome:ARL6:GTP complex | |||||||||
![]() | BBSome:ARL6:GTP complex. Post-processed map. | |||||||||
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![]() | Cilia / ciliopathy / complex / membrane-protein transport / PROTEIN TRANSPORT | |||||||||
機能・相同性 | ![]() BBSome binding / establishment of anatomical structure orientation / BBSome-mediated cargo-targeting to cilium / multi-ciliated epithelial cell differentiation / receptor localization to non-motile cilium / renal tubule development / BBSome / camera-type eye photoreceptor cell differentiation / smoothened binding / establishment of planar polarity ...BBSome binding / establishment of anatomical structure orientation / BBSome-mediated cargo-targeting to cilium / multi-ciliated epithelial cell differentiation / receptor localization to non-motile cilium / renal tubule development / BBSome / camera-type eye photoreceptor cell differentiation / smoothened binding / establishment of planar polarity / photoreceptor connecting cilium / axonemal microtubule / inner ear receptor cell stereocilium organization / patched binding / olfactory bulb development / membrane coat / non-motile cilium assembly / phosphatidylinositol-3-phosphate binding / establishment of epithelial cell apical/basal polarity / protein localization to cilium / regulation of stress fiber assembly / non-motile cilium / motile cilium / protein targeting to membrane / centrosome cycle / ciliary membrane / pericentriolar material / fat cell differentiation / axoneme / centriolar satellite / protein polymerization / cilium assembly / intracellular transport / vesicle-mediated transport / ciliary basal body / protein localization to plasma membrane / axon guidance / intracellular protein transport / protein localization / multicellular organism growth / phospholipid binding / cilium / regulation of protein localization / sensory perception of smell / protein transport / protein-macromolecule adaptor activity / RNA polymerase II-specific DNA-binding transcription factor binding / neuron projection / GTPase activity / centrosome / GTP binding / membrane / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.5 Å | |||||||||
![]() | Singh SK / Gui M | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structure and activation mechanism of the BBSome membrane protein trafficking complex. 著者: Sandeep K Singh / Miao Gui / Fujiet Koh / Matthew Cj Yip / Alan Brown / ![]() 要旨: Bardet-Biedl syndrome (BBS) is a currently incurable ciliopathy caused by the failure to correctly establish or maintain cilia-dependent signaling pathways. Eight proteins associated with BBS ...Bardet-Biedl syndrome (BBS) is a currently incurable ciliopathy caused by the failure to correctly establish or maintain cilia-dependent signaling pathways. Eight proteins associated with BBS assemble into the BBSome, a key regulator of the ciliary membrane proteome. We report the electron cryomicroscopy (cryo-EM) structures of the native bovine BBSome in inactive and active states at 3.1 and 3.5 Å resolution, respectively. In the active state, the BBSome is bound to an Arf-family GTPase (ARL6/BBS3) that recruits the BBSome to ciliary membranes. ARL6 recognizes a composite binding site formed by BBS1 and BBS7 that is occluded in the inactive state. Activation requires an unexpected swiveling of the β-propeller domain of BBS1, the subunit most frequently implicated in substrate recognition, which widens a central cavity of the BBSome. Structural mapping of disease-causing mutations suggests that pathogenesis results from folding defects and the disruption of autoinhibition and activation. | |||||||||
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構造の表示
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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マップデータ | ![]() | 116.4 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 43.2 KB 43.2 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 11.4 KB | 表示 | ![]() |
画像 | ![]() | 199 KB | ||
マスクデータ | ![]() ![]() ![]() ![]() | 125 MB 125 MB 125 MB 125 MB | ![]() | |
Filedesc metadata | ![]() | 10.3 KB | ||
その他 | ![]() ![]() ![]() ![]() ![]() ![]() ![]() | 98.8 MB 115.9 MB 116.4 MB 116.2 MB 116 MB 99.2 MB 99.2 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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文書・要旨 | ![]() | 1.1 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1.1 MB | 表示 | |
XML形式データ | ![]() | 18.4 KB | 表示 | |
CIF形式データ | ![]() | 24.1 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | BBSome:ARL6:GTP complex. Post-processed map. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
+マスク #1
+マスク #2
+マスク #3
+マスク #4
+追加マップ: BBSome:ARL6:GTP complex. Unfiltered map
+追加マップ: BBSome:ARL6:GTP complex. Chimeric map following multibody refinement.
+追加マップ: Post-processed multibody map for the BBSome body
+追加マップ: Post-processed multibody map for the BBSome head
+追加マップ: Post-processed multibody map for the BBS1-ARL6 interaction.
+ハーフマップ: BBSome:ARL6:GTP complex. Half map 1
+ハーフマップ: BBSome:ARL6:GTP complex. Half map 2
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試料の構成要素
+全体 : Bovine BBSome:ARL6:GTP complex
+超分子 #1: Bovine BBSome:ARL6:GTP complex
+超分子 #2: BBSome complex
+超分子 #3: ADP-ribosylation factor-like protein 6
+分子 #1: Bardet-Biedl syndrome 18 protein
+分子 #2: BBS1 domain-containing protein
+分子 #3: Bardet-Biedl syndrome 2 protein homolog
+分子 #4: Bardet-Biedl syndrome 4 protein homolog
+分子 #5: Bardet-Biedl syndrome 5 protein homolog
+分子 #6: Bardet-Biedl syndrome 7 protein homolog
+分子 #7: Tetratricopeptide repeat domain 8
+分子 #8: Bardet-Biedl syndrome 9
+分子 #9: ADP-ribosylation factor-like protein 6
+分子 #10: CALCIUM ION
+分子 #11: GUANOSINE-5'-TRIPHOSPHATE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 0.7 mg/mL | ||||||||||||||||||
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緩衝液 | pH: 7.5 構成要素:
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グリッド | 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: HOLEY / 詳細: unspecified | ||||||||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 293 K / 装置: FEI VITROBOT MARK II / 詳細: Grids were blotted for 2 s with a -2 offset.. |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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特殊光学系 | エネルギーフィルター - 名称: GIF Bioquantum / エネルギーフィルター - スリット幅: 25 eV |
撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 撮影したグリッド数: 2 / 実像数: 9408 / 平均露光時間: 4.0 sec. / 平均電子線量: 56.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | C2レンズ絞り径: 50.0 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 2.4 µm / 最小 デフォーカス(公称値): 1.1 µm / 倍率(公称値): 81000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
-原子モデル構築 1
詳細 | During refinement, the resolution limit was set to 3.5 Angstrom. Secondary structure, Ramachandran and rotamer restraints were applied during refinement. |
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精密化 | 空間: REAL / プロトコル: OTHER / 温度因子: 43.6 / 当てはまり具合の基準: Correlation coefficient |
得られたモデル | ![]() PDB-6vbv: |