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- PDB-6vbv: Structure of the bovine BBSome:ARL6:GTP complex -

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Basic information

Entry
Database: PDB / ID: 6vbv
TitleStructure of the bovine BBSome:ARL6:GTP complex
Components
  • (Bardet-Biedl syndrome ...) x 6
  • ADP-ribosylation factor-like protein 6
  • BBS1 domain-containing protein
  • Tetratricopeptide repeat domain 8
KeywordsPROTEIN TRANSPORT / Cilia / ciliopathy / complex / membrane-protein transport
Function / homology
Function and homology information


BBSome binding / establishment of anatomical structure orientation / BBSome-mediated cargo-targeting to cilium / protein transport from ciliary membrane to plasma membrane / receptor localization to non-motile cilium / multi-ciliated epithelial cell differentiation / BBSome / protein localization to non-motile cilium / renal tubule development / camera-type eye photoreceptor cell differentiation ...BBSome binding / establishment of anatomical structure orientation / BBSome-mediated cargo-targeting to cilium / protein transport from ciliary membrane to plasma membrane / receptor localization to non-motile cilium / multi-ciliated epithelial cell differentiation / BBSome / protein localization to non-motile cilium / renal tubule development / camera-type eye photoreceptor cell differentiation / smoothened binding / establishment of planar polarity / photoreceptor connecting cilium / inner ear receptor cell stereocilium organization / axonemal microtubule / retina layer formation / patched binding / membrane coat / olfactory bulb development / establishment of epithelial cell apical/basal polarity / protein localization to cilium / phosphatidylinositol-3-phosphate binding / regulation of stress fiber assembly / non-motile cilium assembly / regulation of smoothened signaling pathway / non-motile cilium / centrosome cycle / protein targeting to membrane / eating behavior / motile cilium / erythrocyte homeostasis / ciliary membrane / fat cell differentiation / pericentriolar material / B cell homeostasis / axoneme / protein polymerization / cilium assembly / vesicle-mediated transport / axon guidance / protein localization to plasma membrane / intracellular protein transport / brain development / phospholipid binding / centriolar satellite / multicellular organism growth / Wnt signaling pathway / fibrillar center / sensory perception of smell / intracellular protein localization / protein transport / regulation of protein localization / protein-macromolecule adaptor activity / gene expression / RNA polymerase II-specific DNA-binding transcription factor binding / neuron projection / cilium / ciliary basal body / GTPase activity / centrosome / GTP binding / nucleoplasm / membrane / cytoplasm / cytosol
Similarity search - Function
ADP-ribosylation factor-like protein 6 / Bardet-Biedl syndrome 7 protein / Bardet-Biedl syndrome 2 protein / Ciliary BBSome complex subunit 2, C-terminal domain / Ciliary BBSome complex subunit 2, middle region / Ciliary BBSome complex subunit 2, N-terminal / : / : / : / : ...ADP-ribosylation factor-like protein 6 / Bardet-Biedl syndrome 7 protein / Bardet-Biedl syndrome 2 protein / Ciliary BBSome complex subunit 2, C-terminal domain / Ciliary BBSome complex subunit 2, middle region / Ciliary BBSome complex subunit 2, N-terminal / : / : / : / : / : / : / : / Ciliary BBSome complex subunit 2, N-terminal / BBS2 GAE domain / Ciliary BBSome complex subunit 2, middle region / BBS7 hairpin / BBS2 platform domain / BBS2 C-terminal helix bundle / BBS2 hairpin / BBS7 GAE domain / BBS7 platform domain / BBS7 beta-propeller / Bardet-Biedl syndrome 5 protein / DM16 repeat / Cilia BBSome complex subunit 10 / Tetratricopeptide repeat protein 8 / Bardet-Biedl syndrome 5 protein/sex-determination protein fem-3 / : / Bardet-Biedl syndrome 5 protein / Cilia BBSome complex subunit 10 / Bardet-Biedl syndrome 1 protein, GAE domain / Repeats in sea squirt COS41.4, worm R01H10.6, fly CG1126 etc. / Parathyroid hormone-responsive B1 / PTHB1, N-terminal domain / PTHB1, C-terminal domain / : / : / : / PTHB1 N-terminal / PTHB1 GAE domain / PTHB1 platform domain / PTHB1 hairpin / PTHB1 C-terminal helix bundle / Bardet-Biedl syndrome 1 protein / Bardet-Biedl syndrome 1, N-terminal / Ciliary BBSome complex subunit 1 / TPR repeat / Small GTPase superfamily, ARF type / Tetratricopeptide repeat / Small GTPase Arf domain profile. / Tetratricopeptide repeat / Sar1p-like members of the Ras-family of small GTPases / Small GTPase superfamily, ARF/SAR type / ADP-ribosylation factor family / ARF-like small GTPases; ARF, ADP-ribosylation factor / Quinoprotein alcohol dehydrogenase-like superfamily / Tetratricopeptide repeat / TPR repeat region circular profile. / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat / Small GTP-binding protein domain / Tetratricopeptide-like helical domain superfamily / PH-like domain superfamily / P-loop containing nucleotide triphosphate hydrolases / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
GUANOSINE-5'-TRIPHOSPHATE / Bardet-Biedl syndrome 5 protein homolog / Bardet-Biedl syndrome 9 / Bardet-Biedl syndrome 1 / Bardet-Biedl syndrome 7 protein homolog / Tetratricopeptide repeat domain 8 / BBSome interacting protein 1 / ADP-ribosylation factor-like protein 6 / BBSome complex member BBS4 / Bardet-Biedl syndrome 2 protein homolog
Similarity search - Component
Biological speciesBos taurus (domestic cattle)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsSingh, S.K. / Gui, M. / Koh, F. / Yip, M.C.J. / Brown, A.
Funding support United States, 1items
OrganizationGrant numberCountry
Other private United States
CitationJournal: Elife / Year: 2020
Title: Structure and activation mechanism of the BBSome membrane protein trafficking complex.
Authors: Sandeep K Singh / Miao Gui / Fujiet Koh / Matthew Cj Yip / Alan Brown /
Abstract: Bardet-Biedl syndrome (BBS) is a currently incurable ciliopathy caused by the failure to correctly establish or maintain cilia-dependent signaling pathways. Eight proteins associated with BBS ...Bardet-Biedl syndrome (BBS) is a currently incurable ciliopathy caused by the failure to correctly establish or maintain cilia-dependent signaling pathways. Eight proteins associated with BBS assemble into the BBSome, a key regulator of the ciliary membrane proteome. We report the electron cryomicroscopy (cryo-EM) structures of the native bovine BBSome in inactive and active states at 3.1 and 3.5 Å resolution, respectively. In the active state, the BBSome is bound to an Arf-family GTPase (ARL6/BBS3) that recruits the BBSome to ciliary membranes. ARL6 recognizes a composite binding site formed by BBS1 and BBS7 that is occluded in the inactive state. Activation requires an unexpected swiveling of the β-propeller domain of BBS1, the subunit most frequently implicated in substrate recognition, which widens a central cavity of the BBSome. Structural mapping of disease-causing mutations suggests that pathogenesis results from folding defects and the disruption of autoinhibition and activation.
History
DepositionDec 19, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 29, 2020Provider: repository / Type: Initial release
Revision 1.1Mar 6, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

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Assembly

Deposited unit
0: Bardet-Biedl syndrome 18 protein
1: BBS1 domain-containing protein
2: Bardet-Biedl syndrome 2 protein homolog
4: Bardet-Biedl syndrome 4 protein homolog
5: Bardet-Biedl syndrome 5 protein homolog
7: Bardet-Biedl syndrome 7 protein homolog
8: Tetratricopeptide repeat domain 8
9: Bardet-Biedl syndrome 9
3: ADP-ribosylation factor-like protein 6
hetero molecules


Theoretical massNumber of molelcules
Total (without water)508,17012
Polymers507,5669
Non-polymers6033
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area52400 Å2
ΔGint-247 kcal/mol
Surface area160510 Å2

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Components

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Bardet-Biedl syndrome ... , 6 types, 6 molecules 024579

#1: Protein Bardet-Biedl syndrome 18 protein


Mass: 8070.502 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: Eye / Tissue: Retina / References: UniProt: G3N2W1
#3: Protein Bardet-Biedl syndrome 2 protein homolog


Mass: 79911.484 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: Eye / Tissue: Retina / References: UniProt: Q32L13
#4: Protein Bardet-Biedl syndrome 4 protein homolog


Mass: 58289.133 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: Eye / Tissue: Retina / References: UniProt: Q1JQ97
#5: Protein Bardet-Biedl syndrome 5 protein homolog


Mass: 38880.984 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: Eye / Tissue: Retina / References: UniProt: A6QLF9
#6: Protein Bardet-Biedl syndrome 7 protein homolog


Mass: 80471.375 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: Eye / Tissue: Retina / References: UniProt: F1MB52
#8: Protein Bardet-Biedl syndrome 9


Mass: 99230.914 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: Eye / Tissue: Retina / References: UniProt: E1BHJ5

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Protein , 3 types, 3 molecules 183

#2: Protein BBS1 domain-containing protein


Mass: 64939.141 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: Eye / Tissue: Retina / References: UniProt: E1BN34
#7: Protein Tetratricopeptide repeat domain 8


Mass: 56686.406 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (domestic cattle) / Organ: Eye / Tissue: Retina / References: UniProt: F1N4X0
#9: Protein ADP-ribosylation factor-like protein 6


Mass: 21086.486 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Bos taurus (domestic cattle) / Gene: ARL6 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q0IIM2

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Non-polymers , 2 types, 3 molecules

#10: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Ca
#11: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

Component
IDNameTypeDetailsEntity IDParent-IDSource
1Bovine BBSome:ARL6:GTP complexCOMPLEXNative BBSome complex isolated from bovine retina and incubated with recombinant bovine ARL6 in the presence of GTP.#1-#90MULTIPLE SOURCES
2BBSome complexCOMPLEX#1-#81NATURAL
3ADP-ribosylation factor-like protein 6COMPLEX#91RECOMBINANT
Molecular weightValue: 0.5 MDa / Experimental value: NO
Source (natural)
IDEntity assembly-IDOrganismNcbi tax-IDOrganTissue
22Bos taurus (domestic cattle)9913EyeRetina
33Bos taurus (domestic cattle)9913
Source (recombinant)Organism: Escherichia coli BL21(DE3) (bacteria) / Strain: BL21(DE3)
Buffer solutionpH: 7.5
Buffer component
IDConc.NameFormulaBuffer-ID
120 mMHepes1
2220 mMNaCl1
35 mMMgCl21
44 mMbeta-mercaptoethanol1
51 mMGTP1
SpecimenConc.: 0.7 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: unspecified
VitrificationInstrument: FEI VITROBOT MARK II / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 293 K / Details: Grids were blotted for 2 s with a -2 offset.

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 81000 X / Nominal defocus max: 2400 nm / Nominal defocus min: 1100 nm / Cs: 2.7 mm / C2 aperture diameter: 50 µm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingAverage exposure time: 4 sec. / Electron dose: 56 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Num. of grids imaged: 2 / Num. of real images: 9408
EM imaging opticsEnergyfilter name: GIF Bioquantum / Energyfilter slit width: 25 eV

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Processing

EM software
IDNameVersionCategoryDetails
1SPHIREparticle selectioncrYOLO was used to pick particles
2SerialEMimage acquisition
4CTFFIND4.1CTF correction
7Coot0.9model fitting
9RELION3initial Euler assignment
10RELION3final Euler assignment
11RELION3classification
12RELION33D reconstruction
19PHENIX1.17.1-3660model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionResolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 75201 / Algorithm: BACK PROJECTION / Symmetry type: POINT
Atomic model buildingB value: 43.6 / Protocol: OTHER / Space: REAL / Target criteria: Correlation coefficient
Details: During refinement, the resolution limit was set to 3.5 Angstrom. Secondary structure, Ramachandran and rotamer restraints were applied during refinement.

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