Ribosomal protein L1, mitochondrial / Gonadotropin-releasing hormone / Gonadoliberin / Gonadotropin-releasing hormone / Gonadotropin-releasing hormones signature. / : / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Cysteine alpha-hairpin motif superfamily / Peptide chain release factor / PCRF domain ...Ribosomal protein L1, mitochondrial / Gonadotropin-releasing hormone / Gonadoliberin / Gonadotropin-releasing hormone / Gonadotropin-releasing hormones signature. / : / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Cysteine alpha-hairpin motif superfamily / Peptide chain release factor / PCRF domain / PCRF / 28S ribosomal protein S27, mitochondrial / MRPS27/PTCD2 / Mitochondrial 28S ribosomal protein S27 / Ribosomal protein S22, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / Mitochondrial 28S ribosomal protein S31 / : / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal protein S23 / Mitochondrial 28S ribosomal protein S31 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S10, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S24, mitochondrial / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / 28S ribosomal protein S18b, mitochondrial / : / : / Mitochondrial ribosome subunit S24 / Small ribosomal subunit protein uS5m, N-terminal / Small ribosomal subunit protein mS39, PPR / Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / 28S ribosomal protein S25, mitochondrial / : / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L55 superfamily / : / Mitochondrial ribosomal protein L55 / Pentatricopeptide repeat domain / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Mitochondrial ribosomal protein L48 / 39S ribosomal protein L40, mitochondrial / Ribosomal protein S30, mitochondrial / Ribosomal protein L53, mitochondrial / 39S ribosomal protein L53/MRP-L53 / 39S ribosomal protein L42, mitochondrial / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial 28S ribosomal protein S32 / Mitochondrial ribosomal subunit protein / Ribosomal protein L37, mitochondrial / Ribosomal protein L28/L40, mitochondrial / Mitochondrial ribosomal protein L37 / Mitochondrial ribosomal protein L28 / Ribosomal protein 63, mitochondrial / Growth arrest/ DNA-damage-inducible protein-interacting protein 1 / Ribosomal protein L35, mitochondrial / Ribosomal protein L51, mitochondrial / : / Growth arrest and DNA-damage-inducible proteins-interacting protein 1 / Mitochondrial ribosomal subunit / Mitochondrial ribosome protein 63 / Large ribosomal subunit protein bL9m N-terminal domain / Ribosomal protein L37/S30 / Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamily / : / : / Mitochondrial 28S ribosomal protein S30 (PDCD9) / 39S ribosomal protein L52, mitochondrial / Mitoribosomal protein mL52 / : / MRPL44, double-stranded RNA binding domain / : / MRPL44 dsRNA-binding domain / Large ribosomal subunit protein mL44, endonuclease domain / : / Large ribosomal subunit protein bL9m C-terminal domain / Tim44-like domain / Tim44-like domain / Pentatricopeptide (PPR) repeat profile. / Tim44 / : / : / Ribosomal protein L7/L12, oligomerisation / Ribosomal protein S23/S29, mitochondrial / Ribosomal protein L7/L12, oligomerisation domain superfamily / Mitochondrial ribosomal death-associated protein 3 / Ribosomal protein L7/L12 dimerisation domain / Mitochondrial ribosomal protein L46 NUDIX / Ribosomal protein L7/L12 / Ribosomal protein L7/L12, C-terminal / Ribosomal protein L7/L12 C-terminal domain 類似検索 - ドメイン・相同性
Small ribosomal subunit protein uS12m / 28S ribosomal protein S14, mitochondrial / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS25 / 28S ribosomal protein S28, mitochondrial / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein uL3m / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein uS11m / Large ribosomal subunit protein mL62 ...Small ribosomal subunit protein uS12m / 28S ribosomal protein S14, mitochondrial / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS25 / 28S ribosomal protein S28, mitochondrial / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein uL3m / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein uS11m / Large ribosomal subunit protein mL62 / 28S ribosomal protein S21, mitochondrial / Large ribosomal subunit protein mL40 / Large ribosomal subunit protein bL28m / Large ribosomal subunit protein bL20m / Large ribosomal subunit protein mL64 / Large ribosomal subunit protein uL2m / Ribosomal protein / Mitochondrial ribosomal protein S23 / Large ribosomal subunit protein uL1m / Small ribosomal subunit protein uS15m / 28S ribosomal protein S24, mitochondrial / Mitochondrial ribosomal protein S22 / Mitochondrial ribosomal protein S34 / Progonadoliberin / Large ribosomal subunit protein uL4m / Large ribosomal subunit protein mL37 / Large ribosomal subunit protein uL14m / Mitochondrial ribosomal protein L57 / Large ribosomal subunit protein mL42 / Large ribosomal subunit protein mL48 / Large ribosomal subunit protein uL23m / Small ribosomal subunit protein bS16m / 39S ribosomal protein S30, mitochondrial / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein uL22m / Large ribosomal subunit protein bL27m / Mitochondrial ribosomal protein L55 / Large ribosomal subunit protein uL18m / Large ribosomal subunit protein bL35m / Small ribosomal subunit protein mS27 / Large ribosomal subunit protein uL15m / Small ribosomal subunit protein bS6m / Large ribosomal subunit protein mL53 / Large ribosomal subunit protein bL12m / Large ribosomal subunit protein uL30m / 39S ribosomal protein L41, mitochondrial / Small ribosomal subunit protein uS17m / Large ribosomal subunit protein bL34m / Small ribosomal subunit protein mS38 / Large ribosomal subunit protein mL51 / Small ribosomal subunit protein mS29 / Large ribosomal subunit protein uL24m / Large ribosomal subunit protein uL16m / Small ribosomal subunit protein mS31 / Large ribosomal subunit protein bL17m / Large ribosomal subunit protein mL66 / Large ribosomal subunit protein uL11m / Small ribosomal subunit protein uS10m / Large ribosomal subunit protein mL38 / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein bL21m / Small ribosomal subunit protein uS2m / Small ribosomal subunit protein mS33 / Large ribosomal subunit protein mL43 / Large ribosomal subunit protein mL52 / 28S ribosomal protein S35, mitochondrial isoform 1 / Large ribosomal subunit protein uL29m / Large ribosomal subunit protein mL39 / Large ribosomal subunit protein mL44 / Large ribosomal subunit protein mL46 / Coiled-coil-helix-coiled-coil-helix domain containing 1 / Small ribosomal subunit protein uS5m / Large ribosomal subunit protein mL50 / Large ribosomal subunit protein mL45 / Large ribosomal subunit protein mL54 / Large ribosomal subunit protein bL19m / Large ribosomal subunit protein bL9m / Large ribosomal subunit protein bL32m / Small ribosomal subunit protein uS9m / Small ribosomal subunit protein mS39 / Large ribosomal subunit protein uL13m / Peptide chain release factor 1, mitochondrial / Small ribosomal subunit protein mS40 類似検索 - 構成要素
ジャーナル: Science / 年: 2023 タイトル: Molecular basis of translation termination at noncanonical stop codons in human mitochondria. 著者: Martin Saurer / Marc Leibundgut / Hima Priyanka Nadimpalli / Alain Scaiola / Tanja Schönhut / Richard G Lee / Stefan J Siira / Oliver Rackham / René Dreos / Tea Lenarčič / Eva Kummer / ...著者: Martin Saurer / Marc Leibundgut / Hima Priyanka Nadimpalli / Alain Scaiola / Tanja Schönhut / Richard G Lee / Stefan J Siira / Oliver Rackham / René Dreos / Tea Lenarčič / Eva Kummer / David Gatfield / Aleksandra Filipovska / Nenad Ban / 要旨: The genetic code that specifies the identity of amino acids incorporated into proteins during protein synthesis is almost universally conserved. Mitochondrial genomes feature deviations from the ...The genetic code that specifies the identity of amino acids incorporated into proteins during protein synthesis is almost universally conserved. Mitochondrial genomes feature deviations from the standard genetic code, including the reassignment of two arginine codons to stop codons. The protein required for translation termination at these noncanonical stop codons to release the newly synthesized polypeptides is not currently known. In this study, we used gene editing and ribosomal profiling in combination with cryo-electron microscopy to establish that mitochondrial release factor 1 (mtRF1) detects noncanonical stop codons in human mitochondria by a previously unknown mechanism of codon recognition. We discovered that binding of mtRF1 to the decoding center of the ribosome stabilizes a highly unusual conformation in the messenger RNA in which the ribosomal RNA participates in specific recognition of the noncanonical stop codons.
タンパク質・ペプチド: 28S ribosomal protein S5, mitochondrial isoform X2
タンパク質・ペプチド: mS39
タンパク質・ペプチド: uS9m
タンパク質・ペプチド: Mitochondrial ribosomal protein S34
タンパク質・ペプチド: Large ribosomal subunit protein mL43
タンパク質・ペプチド: Mitochondrial ribosomal protein S11
リガンド: MAGNESIUM ION
リガンド: POTASSIUM ION
リガンド: PHENYLALANINE
リガンド: ZINC ION
リガンド: FE2/S2 (INORGANIC) CLUSTER
リガンド: SPERMINE
リガンド: SPERMIDINE
リガンド: N-FORMYLMETHIONINE
リガンド: ADENOSINE-5'-TRIPHOSPHATE
リガンド: GUANOSINE-5'-DIPHOSPHATE
リガンド: water
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超分子 #1: 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA
超分子
名称: 55S mammalian mitochondrial ribosome with mtRF1 and P-site tRNA タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#47, #49-#87, #89 詳細: Mammalian mitochondrial translation termination complex, in vitro reconstituted from porcine mitoribosomal subunits, human mtRF1-3xFLAG, human methionine-tRNA(formyl-methionine), and a ...詳細: Mammalian mitochondrial translation termination complex, in vitro reconstituted from porcine mitoribosomal subunits, human mtRF1-3xFLAG, human methionine-tRNA(formyl-methionine), and a synthetic mRNA (5'-A AUG AGA CAA-3') containing the non-canonical stop codon AGA.
名称: P-site Met-tRNA(fMet) / タイプ: rna / ID: 62 詳細: Corresponds to GenBank sequence ID LC530718.1 (positions 1-71). In vitro transcribed human tRNA with formyl-methionine attached to the CCA-end of the tRNA. コピー数: 1
由来(天然)
生物種: Homo sapiens (ヒト)
分子量
理論値: 22.664498 KDa
配列
文字列:
AGUAAGGUCA GCUAAAUAAG CUAUCGGGCC CAUACCCCGA AAAUGUUGGU UAUACCCUUC CCGUACUACC A
タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: cryoSPARC (ver. 4) 詳細: Homogeneous refinement (Cryosparc v4) of all particles with EMD-2914 (low-pass filtered to 40A) as initial reference
最終 角度割当
タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: cryoSPARC (ver. 4)