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- EMDB-16896: 39S mammalian mitochondrial large ribosomal subunit with mtRF1 an... -

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Basic information

Entry
Database: EMDB / ID: EMD-16896
Title39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA
Map data39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA
Sample
  • Complex: 39S mammalian mitochondrial ribosome with mtRF1 and P-site tRNAMet
    • Protein or peptide: x 53 types
    • RNA: x 4 types
  • Ligand: x 6 types
Keywordsmammalian mitochondrial ribosome / release factor mtRF1 / non-canonical stop codon / RIBOSOME
Function / homology
Function and homology information


Mitochondrial translation elongation / Mitochondrial translation termination / translation release factor activity / mitochondrial transcription / mitochondrial translational elongation / mitochondrial translational termination / translation release factor activity, codon nonspecific / microprocessor complex / translation release factor activity, codon specific / ribonuclease III activity ...Mitochondrial translation elongation / Mitochondrial translation termination / translation release factor activity / mitochondrial transcription / mitochondrial translational elongation / mitochondrial translational termination / translation release factor activity, codon nonspecific / microprocessor complex / translation release factor activity, codon specific / ribonuclease III activity / ribosomal subunit / mitochondrial large ribosomal subunit / peptidyl-tRNA hydrolase / aminoacyl-tRNA hydrolase activity / mitochondrial ribosome / mitochondrial translation / organelle membrane / RNA processing / rescue of stalled ribosome / cellular response to leukemia inhibitory factor / large ribosomal subunit / double-stranded RNA binding / cell junction / cytosolic large ribosomal subunit / negative regulation of translation / nuclear body / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / cell cycle / protein domain specific binding / nucleotide binding / mRNA binding / synapse / positive regulation of DNA-templated transcription / mitochondrion / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Ribosomal protein L1, mitochondrial / Peptide chain release factor / PCRF domain / PCRF / Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / Ribosomal protein L37, mitochondrial / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L48 / 39S ribosomal protein L40, mitochondrial ...Ribosomal protein L1, mitochondrial / Peptide chain release factor / PCRF domain / PCRF / Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / Ribosomal protein L37, mitochondrial / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L48 / 39S ribosomal protein L40, mitochondrial / Mitochondrial ribosomal protein L55 superfamily / Mitochondrial ribosomal protein L37 / Mitochondrial ribosomal protein L55 / Mitochondrial ribosomal protein L46 NUDIX / Ribosomal protein S30, mitochondrial / Ribosomal protein L53, mitochondrial / 39S ribosomal protein L53/MRP-L53 / 39S ribosomal protein L42, mitochondrial / Mitochondrial 28S ribosomal protein S32 / Ribosomal protein L28/L40, mitochondrial / Mitochondrial ribosomal protein L28 / Ribosomal protein L37/S30 / Ribosomal protein 63, mitochondrial / Growth arrest/ DNA-damage-inducible protein-interacting protein 1 / Ribosomal protein L51, mitochondrial / Mitochondrial 28S ribosomal protein S30 (PDCD9) / Growth arrest and DNA-damage-inducible proteins-interacting protein 1 / Mitochondrial ribosomal subunit / Mitochondrial ribosome protein 63 / Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamily / Ribosomal protein L35, mitochondrial / 39S ribosomal protein L52, mitochondrial / Mitoribosomal protein mL52 / MRPL44, double-stranded RNA binding domain / Tim44-like domain / Tim44-like domain / Tim44 / Ribosomal protein L49/IMG2 / Mitochondrial large subunit ribosomal protein (Img2) / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Ribosomal protein L50, mitochondria / Ribosomal protein L27/L41, mitochondrial / Mitochondrial ribosomal protein L27 / Ribosomal subunit 39S / 39S ribosomal protein L46, mitochondrial / Ribosomal protein L7/L12, oligomerisation / Ribosomal protein L7/L12, oligomerisation domain superfamily / Ribosomal protein L7/L12 dimerisation domain / Ribosomal protein L7/L12 / Ribosomal protein L7/L12, C-terminal / Ribosomal protein L7/L12 C-terminal domain / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / 39S ribosomal protein L43/54S ribosomal protein L51 / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein / Phosphatidylethanolamine-binding protein, eukaryotic / PEBP-like superfamily / Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like / Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily / TGS domain profile. / TGS / TGS-like / Peptide chain release factor class I / RF-1 domain / Ribonuclease III, endonuclease domain superfamily / Double stranded RNA-binding domain (dsRBD) profile. / Double-stranded RNA-binding domain / : / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Beta-grasp domain superfamily / NTF2-like domain superfamily / Ribosomal protein L1, 3-layer alpha/beta-sandwich / NUDIX hydrolase-like domain superfamily / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10 / Ribosomal protein L10P / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11, RNA binding domain / Ribosomal protein L11/L12 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L35
Similarity search - Domain/homology
Large ribosomal subunit protein uL3m / Large ribosomal subunit protein mL62 / Large ribosomal subunit protein mL40 / Large ribosomal subunit protein bL28m / 39S ribosomal protein L20, mitochondrial isoform 1 / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein mL64 / 39S ribosomal protein L49, mitochondrial / Large ribosomal subunit protein bL34m / Mitochondrial ribosomal protein L2 ...Large ribosomal subunit protein uL3m / Large ribosomal subunit protein mL62 / Large ribosomal subunit protein mL40 / Large ribosomal subunit protein bL28m / 39S ribosomal protein L20, mitochondrial isoform 1 / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein mL64 / 39S ribosomal protein L49, mitochondrial / Large ribosomal subunit protein bL34m / Mitochondrial ribosomal protein L2 / Ribosomal protein / Large ribosomal subunit protein uL1m / Mitochondrial ribosomal protein L12 / Mitochondrial ribosomal protein L41 / Large ribosomal subunit protein uL4m / Large ribosomal subunit protein mL51 / Large ribosomal subunit protein mL37 / Large ribosomal subunit protein uL14m / Mitochondrial ribosomal protein L57 / Large ribosomal subunit protein mL42 / 39S ribosomal protein L48, mitochondrial / Large ribosomal subunit protein uL23m / 39S ribosomal protein S30, mitochondrial / Mitochondrial ribosomal protein L18 / 39S ribosomal protein L35, mitochondrial / Large ribosomal subunit protein uL15m / Large ribosomal subunit protein mL53 / Large ribosomal subunit protein uL24m / Large ribosomal subunit protein uL16m / Large ribosomal subunit protein bL17m / Large ribosomal subunit protein uL22m / Mitochondrial ribosomal protein S18A / 39S ribosomal protein L27, mitochondrial / Large ribosomal subunit protein uL11m / Large ribosomal subunit protein mL38 / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein bL21m / Large ribosomal subunit protein mL43 / Large ribosomal subunit protein mL52 / Large ribosomal subunit protein uL29m / Mitochondrial ribosomal protein L39 / Large ribosomal subunit protein mL44 / Large ribosomal subunit protein mL46 / Large ribosomal subunit protein uL30m / Large ribosomal subunit protein mL50 / Mitochondrial ribosomal protein L55 / Large ribosomal subunit protein mL45 / Large ribosomal subunit protein mL54 / Large ribosomal subunit protein bL19m / Large ribosomal subunit protein bL9m / Large ribosomal subunit protein bL32m / 39S ribosomal protein L13, mitochondrial / Peptide chain release factor 1, mitochondrial
Similarity search - Component
Biological speciesSus scrofa (pig) / Homo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsSaurer M / Leibundgut M / Scaiola A / Schoenhut T / Ban N
Funding support Switzerland, European Union, 3 items
OrganizationGrant numberCountry
Swiss National Science Foundation179190 Switzerland
Swiss National Science Foundation182341 Switzerland
European Molecular Biology Organization (EMBO)1074-2019European Union
CitationJournal: Science / Year: 2023
Title: Molecular basis of translation termination at noncanonical stop codons in human mitochondria.
Authors: Martin Saurer / Marc Leibundgut / Hima Priyanka Nadimpalli / Alain Scaiola / Tanja Schönhut / Richard G Lee / Stefan J Siira / Oliver Rackham / René Dreos / Tea Lenarčič / Eva Kummer / ...Authors: Martin Saurer / Marc Leibundgut / Hima Priyanka Nadimpalli / Alain Scaiola / Tanja Schönhut / Richard G Lee / Stefan J Siira / Oliver Rackham / René Dreos / Tea Lenarčič / Eva Kummer / David Gatfield / Aleksandra Filipovska / Nenad Ban /
Abstract: The genetic code that specifies the identity of amino acids incorporated into proteins during protein synthesis is almost universally conserved. Mitochondrial genomes feature deviations from the ...The genetic code that specifies the identity of amino acids incorporated into proteins during protein synthesis is almost universally conserved. Mitochondrial genomes feature deviations from the standard genetic code, including the reassignment of two arginine codons to stop codons. The protein required for translation termination at these noncanonical stop codons to release the newly synthesized polypeptides is not currently known. In this study, we used gene editing and ribosomal profiling in combination with cryo-electron microscopy to establish that mitochondrial release factor 1 (mtRF1) detects noncanonical stop codons in human mitochondria by a previously unknown mechanism of codon recognition. We discovered that binding of mtRF1 to the decoding center of the ribosome stabilizes a highly unusual conformation in the messenger RNA in which the ribosomal RNA participates in specific recognition of the noncanonical stop codons.
History
DepositionMar 23, 2023-
Header (metadata) releaseJun 14, 2023-
Map releaseJun 14, 2023-
UpdateNov 15, 2023-
Current statusNov 15, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_16896.png

Downloads & links

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Map

FileDownload / File: emd_16896.map.gz / Format: CCP4 / Size: 476.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA
Voxel sizeX=Y=Z: 1.065 Å
Density
Contour LevelBy AUTHOR: 0.5
Minimum - Maximum-2.4873815 - 3.8000934
Average (Standard dev.)-0.00095234596 (±0.13020763)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions500500500
Spacing500500500
CellA=B=C: 532.5 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: half map A

Fileemd_16896_half_map_1.map
Annotationhalf map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map B

Fileemd_16896_half_map_2.map
Annotationhalf map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Sample components

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Entire : 39S mammalian mitochondrial ribosome with mtRF1 and P-site tRNAMet

EntireName: 39S mammalian mitochondrial ribosome with mtRF1 and P-site tRNAMet
Components
  • Complex: 39S mammalian mitochondrial ribosome with mtRF1 and P-site tRNAMet
    • Protein or peptide: Mitochondrial ribosomal protein L12
    • RNA: E-site tRNA
    • RNA: 16S rRNA
    • RNA: CP Phe-tRNA
    • Protein or peptide: uL22m
    • Protein or peptide: 39S ribosomal protein L23, mitochondrialRibosome
    • Protein or peptide: uL24m
    • Protein or peptide: Mitochondrial ribosomal protein L27
    • Protein or peptide: Mitochondrial ribosomal protein L28
    • Protein or peptide: Mitochondrial ribosomal protein L47
    • Protein or peptide: uL30m
    • Protein or peptide: Mitochondrial ribosomal protein L32
    • Protein or peptide: bL33m
    • Protein or peptide: Mitochondrial ribosomal protein L34
    • Protein or peptide: 39S ribosomal protein L35, mitochondrialRibosome
    • Protein or peptide: Mitochondrial ribosomal protein L2
    • Protein or peptide: ICT1
    • Protein or peptide: Mitochondrial ribosomal protein L4
    • Protein or peptide: Mitochondrial ribosomal protein L9
    • Protein or peptide: Mitochondrial ribosomal protein L10
    • Protein or peptide: Mitochondrial ribosomal protein L11
    • Protein or peptide: 39S ribosomal protein L13, mitochondrialRibosome
    • Protein or peptide: Mitochondrial ribosomal protein L14
    • Protein or peptide: 39S ribosomal protein L15, mitochondrialRibosome
    • Protein or peptide: uL16m
    • Protein or peptide: bL17m
    • Protein or peptide: Mitochondrial ribosomal protein L18
    • Protein or peptide: 39S ribosomal protein L19, mitochondrialRibosome
    • Protein or peptide: Mitochondrial ribosomal protein L20
    • Protein or peptide: Mitochondrial ribosomal protein L21
    • Protein or peptide: 39S ribosomal protein L52, mitochondrialRibosome
    • Protein or peptide: Mitochondrial ribosomal protein L53
    • Protein or peptide: mL54
    • Protein or peptide: bL31m
    • Protein or peptide: Mitochondrial ribosomal protein L57
    • Protein or peptide: mL62 (ICT1)
    • Protein or peptide: mL64Microsoft Macro Assembler
    • Protein or peptide: Mitochondrial ribosomal protein S18A
    • Protein or peptide: 39S ribosomal protein S30, mitochondrialRibosome
    • Protein or peptide: 39S ribosomal protein L1, mitochondrialRibosome
    • Protein or peptide: Ribosomal protein
    • Protein or peptide: Mitochondrial ribosomal protein L37
    • Protein or peptide: Mitochondrial ribosomal protein L38
    • Protein or peptide: Mitochondrial ribosomal protein L39
    • Protein or peptide: Mitochondrial ribosomal protein L40
    • Protein or peptide: Mitochondrial ribosomal protein L41
    • Protein or peptide: mL42
    • Protein or peptide: 39S ribosomal protein L43, mitochondrial isoform X2Ribosome
    • Protein or peptide: mL44
    • Protein or peptide: Mitochondrial ribosomal protein L45
    • Protein or peptide: Mitochondrial ribosomal protein L46
    • Protein or peptide: 39S ribosomal protein L48, mitochondrialRibosome
    • Protein or peptide: 39S ribosomal protein L49, mitochondrialRibosome
    • Protein or peptide: Mitochondrial ribosomal protein L50
    • Protein or peptide: Mitochondrial ribosomal protein L51
    • RNA: P-site Met-tRNA(fMet)
    • Protein or peptide: Peptide chain release factor 1, mitochondrial
  • Ligand: MAGNESIUM ION
  • Ligand: POTASSIUM IONPotassium
  • Ligand: PHENYLALANINE
  • Ligand: ZINC ION
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: N-FORMYLMETHIONINE

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Supramolecule #1: 39S mammalian mitochondrial ribosome with mtRF1 and P-site tRNAMet

SupramoleculeName: 39S mammalian mitochondrial ribosome with mtRF1 and P-site tRNAMet
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#57
Details: Mammalian mitochondrial translation termination complex, in vitro reconstituted from porcine mitoribosomal subunits, human mtRF1-3xFLAG, human methionine-tRNA(formyl-methionine), and a ...Details: Mammalian mitochondrial translation termination complex, in vitro reconstituted from porcine mitoribosomal subunits, human mtRF1-3xFLAG, human methionine-tRNA(formyl-methionine), and a synthetic mRNA (5'-A AUG AGA CAA-3') containing the non-canonical stop codon AGA. Refined with focus on the large subunit.
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver / Organelle: Mitochondria / Location in cell: Mitochondrial matrix
Molecular weightTheoretical: 1.5 MDa

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Macromolecule #1: Mitochondrial ribosomal protein L12

MacromoleculeName: Mitochondrial ribosomal protein L12 / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 21.268975 KDa
SequenceString: MLPAAASSLW GPCFGLRAAA LRVARHQGPR LCGVRLMRCS SHRKGEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKT LKIQDVGFMP MGAVAPGAPP AAAAPEAAEE DLPKRKEQTH FTVRLTEAKP VDKVKLIKEI KSHIQGINLV Q AKKLVESL ...String:
MLPAAASSLW GPCFGLRAAA LRVARHQGPR LCGVRLMRCS SHRKGEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKT LKIQDVGFMP MGAVAPGAPP AAAAPEAAEE DLPKRKEQTH FTVRLTEAKP VDKVKLIKEI KSHIQGINLV Q AKKLVESL PQEIKANVPK AEAEKIKAAL EAVGGTVVLE

UniProtKB: Mitochondrial ribosomal protein L12

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Macromolecule #5: uL22m

MacromoleculeName: uL22m / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 24.313357 KDa
SequenceString: MAAAVLERLG GLWMQNLRGK LALRLLSSHS ILPQSHIHTS ASVEISRKWE KKNKIVYPPQ LPGEPRRPAE IYHCRRQIKY SKDKMWYLA KLIRGMSIDQ ALAQLEFSDK KGAQIIKEVL LEAQDMAVRD HNVEFRSNLY VAESTSGRGQ YLKRIRYHGR G RFGIMEKV ...String:
MAAAVLERLG GLWMQNLRGK LALRLLSSHS ILPQSHIHTS ASVEISRKWE KKNKIVYPPQ LPGEPRRPAE IYHCRRQIKY SKDKMWYLA KLIRGMSIDQ ALAQLEFSDK KGAQIIKEVL LEAQDMAVRD HNVEFRSNLY VAESTSGRGQ YLKRIRYHGR G RFGIMEKV FCHYFVKLVE GPPPPREAPK TAVAHAREYI QELRNRTITH AL

UniProtKB: Large ribosomal subunit protein uL22m

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Macromolecule #6: 39S ribosomal protein L23, mitochondrial

MacromoleculeName: 39S ribosomal protein L23, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 17.685244 KDa
SequenceString:
M(AYA)RNVLYPLY QLGNPQLRVF RTNFFIQLVR PGTAQPEDTV QFRIPMEMTR VDLRNYLERI YNVPVAAVRT RVQYGS NRR RDHRNIRIKK PDYKVAYVQL ALGQTFTFPD LFPERKGASV DVDVRDQVLE DQRQKHSPDP RRGGVPGWFG L

UniProtKB: Large ribosomal subunit protein uL23m

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Macromolecule #7: uL24m

MacromoleculeName: uL24m / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 24.893529 KDa
SequenceString: MRLSALLALA SKVTLPPNYR YGMSRPGSLS DKRKNPPGTR RRRVPVEPIS DEDWHLFCGD KVEILEGKDA GKQGKVVQVI RQRNWVVVE GLNTHYRYVG KTVDYRGTMI PSEAPLLHNQ VKLVDPTDRK PTDVEWRFTE AGERVRVSTR SGRIIPKPEF P RADGIVPE ...String:
MRLSALLALA SKVTLPPNYR YGMSRPGSLS DKRKNPPGTR RRRVPVEPIS DEDWHLFCGD KVEILEGKDA GKQGKVVQVI RQRNWVVVE GLNTHYRYVG KTVDYRGTMI PSEAPLLHNQ VKLVDPTDRK PTDVEWRFTE AGERVRVSTR SGRIIPKPEF P RADGIVPE TWIDGPKDTS VEDALEKTYV PRLKTLEEEV MEAMGIQETR RHKKVYWY

UniProtKB: Large ribosomal subunit protein uL24m

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Macromolecule #8: Mitochondrial ribosomal protein L27

MacromoleculeName: Mitochondrial ribosomal protein L27 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 15.943688 KDa
SequenceString:
MALAVLALRT RAAVTALLSP PQAAALAVRY ASKKTGGSSK NLGGKSPGKR FGIKKMEGHY VHAGNILATQ RHFRWHPGAH VGLGKNKCL YALEEGVVRY TKEVYVPNPS NSEAVDLVTR LPQGAVLYKT FVHVVPAKPE GTFKLVAML

UniProtKB: 39S ribosomal protein L27, mitochondrial

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Macromolecule #9: Mitochondrial ribosomal protein L28

MacromoleculeName: Mitochondrial ribosomal protein L28 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 30.148953 KDa
SequenceString: MPLHKVPVGL WKQLRLWEGI YSRLPRHYLR SLEEARTPTP VHYRPHGAKF KINPKNWQRE RVEDVPIPVH YPPESQLGLW GGEGWVLGH RYVNNDKLSK RVRKVWKPQL FQRELYSEIL DKRFTVTVTM RTLDLIDQAC GFDFYILKTP KEDLCSKFGM D LKRGMLLR ...String:
MPLHKVPVGL WKQLRLWEGI YSRLPRHYLR SLEEARTPTP VHYRPHGAKF KINPKNWQRE RVEDVPIPVH YPPESQLGLW GGEGWVLGH RYVNNDKLSK RVRKVWKPQL FQRELYSEIL DKRFTVTVTM RTLDLIDQAC GFDFYILKTP KEDLCSKFGM D LKRGMLLR LARQDPQLHP DDPARRAAIY DRYKAFVIPE AEAEWVGLTL DEAVEKQRLL EEKDPIPLFK IFVEELLGQL QQ QALSEPA VVQTRASRK

UniProtKB: Large ribosomal subunit protein bL28m

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Macromolecule #10: Mitochondrial ribosomal protein L47

MacromoleculeName: Mitochondrial ribosomal protein L47 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 29.894867 KDa
SequenceString: MAAAGLAVFC RRVSAALKAC RLLIRPQAPP STRFSPSLLP KNTPNVTSFH QFRIFHTTFS RRGLEEFFDD PKNWGEEKVK SGASWTCQQ LRNKSNEDLH KLWYVLLKER NMLLTLEQEA KRQRLPMPSP ERLEKVVDSM DALDKVVQER EDALRLLQTG Q EKARPGAW ...String:
MAAAGLAVFC RRVSAALKAC RLLIRPQAPP STRFSPSLLP KNTPNVTSFH QFRIFHTTFS RRGLEEFFDD PKNWGEEKVK SGASWTCQQ LRNKSNEDLH KLWYVLLKER NMLLTLEQEA KRQRLPMPSP ERLEKVVDSM DALDKVVQER EDALRLLQTG Q EKARPGAW RRDIFGRIIW HKFKQWPIPW YLNKKYNRKR FFAMPYVERF VRMRIEKQAR IKARKRSLER KKEKFLQEKF PH LSETQKS SHV

UniProtKB: Large ribosomal subunit protein uL29m

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Macromolecule #11: uL30m

MacromoleculeName: uL30m / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 18.9211 KDa
SequenceString:
HPPRQDFLVR RQPHRNRTVT KGMESLICTD WIRHKFTRSR IPDKVFQPSP EDHEKYGGDP QYPHKLHIVT RIKSTKRRPY WEKDIIKML GLEKAHTPQV HKNIPSVNAK LKVVKHLIRI KPLKLPQGLP TEEDMANTCL KSNGELVVRW LLNPANQEAR K S

UniProtKB: Large ribosomal subunit protein uL30m

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Macromolecule #12: Mitochondrial ribosomal protein L32

MacromoleculeName: Mitochondrial ribosomal protein L32 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 23.567215 KDa
SequenceString: MLEVGVGPRP DGRGFWASNM ASAMLVLVVP PWPAARGLLR NWWEQLQRKL QHNRLGLPLH PWGPALAVQG PAICTEPAND TNGSKAISS LLDSVFWMAA PKNRRSIEVN RCRRRNPQKL IKVKNNIDVC PECGHLKQKH ILCGYCYEKV RKETAEIRRQ M GKQEGGPF ...String:
MLEVGVGPRP DGRGFWASNM ASAMLVLVVP PWPAARGLLR NWWEQLQRKL QHNRLGLPLH PWGPALAVQG PAICTEPAND TNGSKAISS LLDSVFWMAA PKNRRSIEVN RCRRRNPQKL IKVKNNIDVC PECGHLKQKH ILCGYCYEKV RKETAEIRRQ M GKQEGGPF RAPTTETVVL YSGETPSEQD QGKRIIERER KRPSWFTQN

UniProtKB: Large ribosomal subunit protein bL32m

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Macromolecule #13: bL33m

MacromoleculeName: bL33m / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 7.508097 KDa
SequenceString:
MFLSAVTFAK SKSKTILVKM MSQAGTGFSF NTKRSRLREK LTLLHYDPVV KKKVLFVEQK KIRSL

UniProtKB: Large ribosomal subunit protein bL33m

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Macromolecule #14: Mitochondrial ribosomal protein L34

MacromoleculeName: Mitochondrial ribosomal protein L34 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 10.871729 KDa
SequenceString:
MAFLARSVGR LLDPVSRSAA LVGGRWLQPQ AWLGFPDTWG LPAMQQTRGK ARGNEYQPSN IKRKHKHGWV RRLRTPTGVQ VILRRMHKG RKSLSH

UniProtKB: Large ribosomal subunit protein bL34m

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Macromolecule #15: 39S ribosomal protein L35, mitochondrial

MacromoleculeName: 39S ribosomal protein L35, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 21.548543 KDa
SequenceString:
MAASAFAGAV RAATGILRPL HILASSAYQN CAKNACLSSV LSSRHFSHIQ TSALLSAPRL ITSVRNLMCG PTAPVLNRVA PLLPHVLKP PVRTVTYFSS RKGKRKTVKA VIYRFLRLHS GLWLRRKAGY KKKLWKKTAA RKRRLREFVF CNKTQSKLLD K MTTSFWKR RNWYADDPYQ KYQDRTNLKV

UniProtKB: 39S ribosomal protein L35, mitochondrial

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Macromolecule #16: Mitochondrial ribosomal protein L2

MacromoleculeName: Mitochondrial ribosomal protein L2 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 33.270934 KDa
SequenceString: MALRVLTRAL SSLSLTPRIA VAPGLNLLPA VQVTNNVLLT LPSGLMSLPC RPILTSVALS ATSVSWKSRT KYTVMPVKMR KSGGRNHTG QIQVHGIGGG HKQRYRMIDF LRFRPEHESK PGPFEEKVIA VRYDPCRSAD IALVAGGNRK RWIIATENMK A GDTVLNSD ...String:
MALRVLTRAL SSLSLTPRIA VAPGLNLLPA VQVTNNVLLT LPSGLMSLPC RPILTSVALS ATSVSWKSRT KYTVMPVKMR KSGGRNHTG QIQVHGIGGG HKQRYRMIDF LRFRPEHESK PGPFEEKVIA VRYDPCRSAD IALVAGGNRK RWIIATENMK A GDTVLNSD HIGRMAVAAR EGDAHPLGAL PVGTLINNVE SEPGRGAQYI RAAGTCGVLL RKVNGTAIIQ LPSKRQMQVL ET CIATVGR VSNVDHNKRV IGKAGRNRWL GKRPNSGLWQ RKGGWAGRKI RPLPPMKSYV KLPSAAAQS

UniProtKB: Mitochondrial ribosomal protein L2

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Macromolecule #17: ICT1

MacromoleculeName: ICT1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 43.70827 KDa
SequenceString: MAPAALLPSW GQRGAAAGGG GKAWEGRRRN ALKAEWRGAQ RCSGRALLGL TMPGWGLLGR AGARVLGCGA DGLGASRGLG NRTDICLLV RSLHGKSVTW WDEHLSEENV PFVKQLVSDE NKAQLASKLC PLKDEPWPIH PWEPGSSRVG LIALKLGMMP L WTKDGQKH ...String:
MAPAALLPSW GQRGAAAGGG GKAWEGRRRN ALKAEWRGAQ RCSGRALLGL TMPGWGLLGR AGARVLGCGA DGLGASRGLG NRTDICLLV RSLHGKSVTW WDEHLSEENV PFVKQLVSDE NKAQLASKLC PLKDEPWPIH PWEPGSSRVG LIALKLGMMP L WTKDGQKH VVTLLQVQDC HVLKYTPKEN HNGRMAALTV GGKTVSHFHK SASILEFYQE LGLPPKQKVK IFNVTENAVI KP GTPLYAA HFRPGQYVDV TAKTIGKGFQ GVMRRWGFKG QPATHGQTKT HRRPGAISTG DVARVWPGTK MPGQLGNIDR TAF GLKVWR INTKHNIIYV NGSVPGHKNC LVKIKDSKLP AYKDFCKNLP FPTYFPDGDE EALPEDLYDE NVCQPGAPSI TFT

UniProtKB: Large ribosomal subunit protein uL3m

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Macromolecule #18: Mitochondrial ribosomal protein L4

MacromoleculeName: Mitochondrial ribosomal protein L4 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 33.251469 KDa
SequenceString: MLQLVRAGAR TWFRPSGCRG LNTLAEEAVQ QAEKPESVAS LGLQPPVLRK CELPVPAHRR PVQAWIESLR GYEQERVGLT ELHPDVFST APRLDILHQV AIWQKNFKRI SYAKTKTRAE VRGGGRKPWV QKGSGRARHG SIRSPIWRGG GVAHGPRGPT S YYYMLPMK ...String:
MLQLVRAGAR TWFRPSGCRG LNTLAEEAVQ QAEKPESVAS LGLQPPVLRK CELPVPAHRR PVQAWIESLR GYEQERVGLT ELHPDVFST APRLDILHQV AIWQKNFKRI SYAKTKTRAE VRGGGRKPWV QKGSGRARHG SIRSPIWRGG GVAHGPRGPT S YYYMLPMK VRVQGLKVAL TVKLAQDDLH IVDSLELPTA DPQYLIELAR YRRWGDSVLL VDLEHEDMPQ NVVAATSGLK TF NLVPAVG LNVHSMLKHQ TLVLTLPTVA FLEEKLLWHN SRYTPLYPFR LPYCDFP

UniProtKB: Large ribosomal subunit protein uL4m

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Macromolecule #19: Mitochondrial ribosomal protein L9

MacromoleculeName: Mitochondrial ribosomal protein L9 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 30.373449 KDa
SequenceString: MAAAACTAAG GGLLRAGLER LLRGGVRALL RPRLEGVTPR SERAFSLSLS RSTVIVERWW KVPLAGEGRK PRLHRRHRVY KLVEDTKHR PKDNLELVLT QSVEELGVRG DLVSVKKSVG RNRLLPQGLA VYASPENKKL FEEEKLLRQE GKLEKLQTKA G EATVKFLR ...String:
MAAAACTAAG GGLLRAGLER LLRGGVRALL RPRLEGVTPR SERAFSLSLS RSTVIVERWW KVPLAGEGRK PRLHRRHRVY KLVEDTKHR PKDNLELVLT QSVEELGVRG DLVSVKKSVG RNRLLPQGLA VYASPENKKL FEEEKLLRQE GKLEKLQTKA G EATVKFLR RCHLEVGMKN NVKWELNPEI VARHFLRNLG VVVAPHALKL PEEPITQRGE YWCEVTVNGL DTVRVPMSVV NF ERPKTKR YKYWLAQQAA KGDVPTSSQM I

UniProtKB: Large ribosomal subunit protein bL9m

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Macromolecule #20: Mitochondrial ribosomal protein L10

MacromoleculeName: Mitochondrial ribosomal protein L10 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 29.238285 KDa
SequenceString: MSSQAFRRHP PQGRLPTFQT VRYGSKAVTR HRRVMHFERQ KLMAVTEYIP PKPTINPRCL PPPPTPPQEE TGLVRLLRRE IAAVFRDNR MIAVCQHVAL SAEDKLLLRH QLRKHKILMK IFPNQVLKPF LEESKYQNLL PLFVGHNLLL VSEEPKVKEM V RILKGIPF ...String:
MSSQAFRRHP PQGRLPTFQT VRYGSKAVTR HRRVMHFERQ KLMAVTEYIP PKPTINPRCL PPPPTPPQEE TGLVRLLRRE IAAVFRDNR MIAVCQHVAL SAEDKLLLRH QLRKHKILMK IFPNQVLKPF LEESKYQNLL PLFVGHNLLL VSEEPKVKEM V RILKGIPF LPLLGGCIDD TILSRQGFIN YSKLPSLALV QGELVGGLTF LTAQTYSMLQ HQPRQLTALL DQYVKQQQEG HP AVPASGQ PEPPDPVPDP

UniProtKB: Large ribosomal subunit protein uL10m

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Macromolecule #21: Mitochondrial ribosomal protein L11

MacromoleculeName: Mitochondrial ribosomal protein L11 / type: protein_or_peptide / ID: 21 / Details: NCBI Reference Sequence: XP_003122536.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 20.809271 KDa
SequenceString: MSKLSRATRA LKKPEASGMI RAIVRAGQAR PGPPLGPILG QRGVSINQFC KEFNEKTKDI KEGIPLPTKI FVKPDRTFEI KIGQPTVSY FLKAAAGIEK GARHTGKEVA GLVTLKHVYE IARVKAQDDA FALQDVPLSS VVRSIIGSAR SLGIRVVKDL S SEELAAFQ ...String:
MSKLSRATRA LKKPEASGMI RAIVRAGQAR PGPPLGPILG QRGVSINQFC KEFNEKTKDI KEGIPLPTKI FVKPDRTFEI KIGQPTVSY FLKAAAGIEK GARHTGKEVA GLVTLKHVYE IARVKAQDDA FALQDVPLSS VVRSIIGSAR SLGIRVVKDL S SEELAAFQ KERALFLAAQ READLAAQAE AAKK

UniProtKB: Large ribosomal subunit protein uL11m

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Macromolecule #22: 39S ribosomal protein L13, mitochondrial

MacromoleculeName: 39S ribosomal protein L13, mitochondrial / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 22.988566 KDa
SequenceString: MRRDTSWILR KGRSCCWGNM (SAC)SFSKAPQQW ATFARVWYLL DGKMQPPGKL AAMASVKLQG LHKPVYHQLS DCGDHV VIM NTRHIAFSGN KWEQKVYSSH TGYPGGFRQV TAAQLHQKDP VAIVKLAIYG MLPKNLHRRT MMQRLHLFPD EDIPEDI LK NLVEELPQPR ...String:
MRRDTSWILR KGRSCCWGNM (SAC)SFSKAPQQW ATFARVWYLL DGKMQPPGKL AAMASVKLQG LHKPVYHQLS DCGDHV VIM NTRHIAFSGN KWEQKVYSSH TGYPGGFRQV TAAQLHQKDP VAIVKLAIYG MLPKNLHRRT MMQRLHLFPD EDIPEDI LK NLVEELPQPR KVPRRLDEYT QEEIEAFPRV WSPPEDYRL

UniProtKB: 39S ribosomal protein L13, mitochondrial

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Macromolecule #23: Mitochondrial ribosomal protein L14

MacromoleculeName: Mitochondrial ribosomal protein L14 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 34.79657 KDa
SequenceString: MVRGWGQRPA PLGPAGPGTS GSRRRLKGGD AQPSPGFFFG WWWDPQRKER GPGKGRVLTP RRRLGSSSEP RVGAGRGWGR GRGSRWAAR APGSCGGRGC VWGRGSGRRE GEEEGRGHEG PTPHASSRKA RAHSQIAARP AGSDAQAQDY RPRGGEGGPG P VGPAAGYS ...String:
MVRGWGQRPA PLGPAGPGTS GSRRRLKGGD AQPSPGFFFG WWWDPQRKER GPGKGRVLTP RRRLGSSSEP RVGAGRGWGR GRGSRWAAR APGSCGGRGC VWGRGSGRRE GEEEGRGHEG PTPHASSRKA RAHSQIAARP AGSDAQAQDY RPRGGEGGPG P VGPAAGYS RAAKAGLPSR DCMAFCTGLR GPFAQVSRAL SQRCFSTTGS LSAIQKMTRV RVVDNSALGT TPYHRPPRCI HV YNKTGVG KVGDRILLAI RGQKKKALIV GHRMPGPRMT PRFDSNNVVL LEDNGNPVGT RIKTPIPSSL RQREGEFSKV LAI AQNFV

UniProtKB: Large ribosomal subunit protein uL14m

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Macromolecule #24: 39S ribosomal protein L15, mitochondrial

MacromoleculeName: 39S ribosomal protein L15, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 33.481723 KDa
SequenceString: MGGPVQGGGP RALDLLRALP RVSLANLKPN PGSRKPERRP RGRRRGRKCG RGHKGERQRG TRPRLGFEGG QTPFYLRIPK YGFNEGHSF RHQYQPLSLN RLQYLIDLGR VDPTQPIDLT QLVNGRGVTI QPSKRDYGVQ LVEEGADTFK AKVNIEVQMA S ELAIAAIE ...String:
MGGPVQGGGP RALDLLRALP RVSLANLKPN PGSRKPERRP RGRRRGRKCG RGHKGERQRG TRPRLGFEGG QTPFYLRIPK YGFNEGHSF RHQYQPLSLN RLQYLIDLGR VDPTQPIDLT QLVNGRGVTI QPSKRDYGVQ LVEEGADTFK AKVNIEVQMA S ELAIAAIE KNGGVVTTAF YDPRSLEILC KPVPFFLRGQ PIPKRMLPPE ALVPYYTDAK NRGYLADPAR FPEARLELAR KY GYVLPDI TKDELFKMLS TRKDPRQIFF GLAPGWVVNM ADKKILKPTD ENLLKYYSS

UniProtKB: Large ribosomal subunit protein uL15m

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Macromolecule #25: uL16m

MacromoleculeName: uL16m / type: protein_or_peptide / ID: 25 / Details: NCBI Reference Sequence: NP_001231896.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 28.680531 KDa
SequenceString: MWRLLARACA PLLRAPLSDS WAAPSATAGF KTLLPVPTFE DVAIPEKPKL RFVERVPLVP KVRRERKNLS DIRGPSTEAT EFTEGRFAI LALGGGYLHW GHFEMMRLTI NRSMDPKNMF ALWRVPAPFK PITRKGMGQR MGGGKGAIDH YVTPVKAGRL I VEMGGRCE ...String:
MWRLLARACA PLLRAPLSDS WAAPSATAGF KTLLPVPTFE DVAIPEKPKL RFVERVPLVP KVRRERKNLS DIRGPSTEAT EFTEGRFAI LALGGGYLHW GHFEMMRLTI NRSMDPKNMF ALWRVPAPFK PITRKGMGQR MGGGKGAIDH YVTPVKAGRL I VEMGGRCE FKEVQGFLDL VAHKLPFPAK AVSRETLEKM RKDQEERERN NQNPWTFERI ATANMLGIRK VLSPYDLTHK GR YWGKFYT PERV

UniProtKB: Large ribosomal subunit protein uL16m

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Macromolecule #26: bL17m

MacromoleculeName: bL17m / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 19.268178 KDa
SequenceString:
MRLSVAAAIS HGRVFRRLGL GPESRIHLLQ NLLTGLVRHE RIEASWARVD ELRGYAEKLI DYGKLGDTNE RAMRMADFWL TEKDLIPKL FQVLAPRFQG QNGGYTRMLQ IPNRNEQDRA KMAVIEYKGN CLPPLPLPRR DSNLTLLNQL LQGLRQDQEA S SHTAQTPA V

UniProtKB: Large ribosomal subunit protein bL17m

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Macromolecule #27: Mitochondrial ribosomal protein L18

MacromoleculeName: Mitochondrial ribosomal protein L18 / type: protein_or_peptide / ID: 27 / Details: NCBI Reference Sequence: XP_003121157.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 21.422613 KDa
SequenceString:
MVLRSSVPLK QVAHARLHFL VPSRKGCRAA ALSTSCKPAT NPETDPVENE AVAPEFTNRN PRNLELLAVA RKERGWGTVW PSREFWHRL RVIRTQHHIE ALVEHRNGQV VVSASTREWA IKKHLYSTRN VVACESVGRV LAERCLEAGI NFMVYHPTPW E AASDSIKR LQHAMTEGGV VLREPRRIYE

UniProtKB: Mitochondrial ribosomal protein L18

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Macromolecule #28: 39S ribosomal protein L19, mitochondrial

MacromoleculeName: 39S ribosomal protein L19, mitochondrial / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 34.528809 KDa
SequenceString: MGVVVSTQRA GMAASITRGS WAAVGLGQSF PPTRALLPAP ASVICRTLAG SGRQQVTGSS EPGAFQPPPK PVIVDKRRPQ RPETRFLSP EFIPPRGRTN PLKFQIERKD MLERRKILHI PEFYVGSILR VTTADPYASA KTSQFLGICI QRSGSGLGAT F ILRNTIEG ...String:
MGVVVSTQRA GMAASITRGS WAAVGLGQSF PPTRALLPAP ASVICRTLAG SGRQQVTGSS EPGAFQPPPK PVIVDKRRPQ RPETRFLSP EFIPPRGRTN PLKFQIERKD MLERRKILHI PEFYVGSILR VTTADPYASA KTSQFLGICI QRSGSGLGAT F ILRNTIEG QGVEICFELY NPRIQEIQVV KLEKRLDNSL LYLRDALPEY STFDVNMKPI TQEFNQEVPV NQLKVKMKPK PW SKRWERP KFNIKGIRFD LCLTEEQMRE AQKWSQPWLE FDMMREYDTS KIEAAIWDEI EASKNS

UniProtKB: Large ribosomal subunit protein bL19m

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Macromolecule #29: Mitochondrial ribosomal protein L20

MacromoleculeName: Mitochondrial ribosomal protein L20 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 17.554793 KDa
SequenceString:
MVFLTVPLWL RSRVTDRYWR VQEVLKHARH FRGRKNRCYR LAVRAVTRAF VKCTRARRLK KRSLRTLWIN RITAASQEHG LKYPAFIIN LIKCQVELNR KVLADLAIYE PKTFKSLAAL AKRRREEGFA AALGDGKEPD GIFSRVVQHR

UniProtKB: 39S ribosomal protein L20, mitochondrial isoform 1

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Macromolecule #30: Mitochondrial ribosomal protein L21

MacromoleculeName: Mitochondrial ribosomal protein L21 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 23.154861 KDa
SequenceString: MAAAVAASVL QGTFGRLVSA YSRSVLRTSR PGTAPPWSAP RRLSSQHTSS LRGCVPKTSL GSPPWPDIVL PDPVEETRHH AEVVGQVNE LIAAGRYGRL FAVVHFAGHQ WKVTAEDLIL IENELDVACG ERIRLEKVLL VGADNFTLLG KPLLGKELVR V EATVIEKT ...String:
MAAAVAASVL QGTFGRLVSA YSRSVLRTSR PGTAPPWSAP RRLSSQHTSS LRGCVPKTSL GSPPWPDIVL PDPVEETRHH AEVVGQVNE LIAAGRYGRL FAVVHFAGHQ WKVTAEDLIL IENELDVACG ERIRLEKVLL VGADNFTLLG KPLLGKELVR V EATVIEKT ESWPKINMKF QKRKNYQRKR ITVNPQTVLR INSIEIAPCL C

UniProtKB: Large ribosomal subunit protein bL21m

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Macromolecule #31: 39S ribosomal protein L52, mitochondrial

MacromoleculeName: 39S ribosomal protein L52, mitochondrial / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 17.989559 KDa
SequenceString:
MAGPVIENQI QDGYFRLPPQ RPAWPSRPRP FPALRLSMAA WGILLSSVRR LHCGTAAQAG SQWRLQQGFA ANPSGYGPLT ELPDWSYAD GRPAPPMKGQ LRRKAQREKF ARRVVLLSQE MDAGLQAWQL RQQEKLQEEE GKQKNALKSK GALLQNPQPS Q

UniProtKB: Large ribosomal subunit protein mL52

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Macromolecule #32: Mitochondrial ribosomal protein L53

MacromoleculeName: Mitochondrial ribosomal protein L53 / type: protein_or_peptide / ID: 32 / Details: NCBI Reference Sequence: XP_020942999.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 12.057784 KDa
SequenceString:
M(AYA)AALARLGL RAVKQVRVQF CPFEKNVEST RTFLQAVSSE KVRCTNLNCS VIADVRHDGS EPCVDVLFGD GHRLIM RGA HLTAQEMLTA FASHIQARGA AGSGDKPSAS TGR

UniProtKB: Large ribosomal subunit protein mL53

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Macromolecule #33: mL54

MacromoleculeName: mL54 / type: protein_or_peptide / ID: 33 / Details: NCBI Reference Sequence: XP_003123104.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 15.957357 KDa
SequenceString:
MAARRLFGAA RSWAAWRAWE LSDAAVSGRL HVRNYAKRPV IKGGKGGKGA VVGEALKDPE VCTDPFRLTT HAMGVNIYKE GQDVVLKPD SEYPEWLFEM NVGPPKKLEE LDPETREYWR LLRKHNIWRH NRLSKNRKF

UniProtKB: Large ribosomal subunit protein mL54

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Macromolecule #34: bL31m

MacromoleculeName: bL31m / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 15.156537 KDa
SequenceString:
MAAKGSLLRL LWQRVVTGAA PESCRHLYTS SWRADCNRAL LTRLHRQTYA RLYPVLLVKQ DGSTIHIRYR EPRRMLTMPV DLDSLSPEE RRARFRKREA KFKEKKEEPE LSDDFDVEQY KRFWTKK

UniProtKB: Mitochondrial ribosomal protein L55

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Macromolecule #35: Mitochondrial ribosomal protein L57

MacromoleculeName: Mitochondrial ribosomal protein L57 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 12.001915 KDa
SequenceString:
MFLTALLRRN RIPGRQWIGK HRRPRPVSAQ AKQNMIRRLE TEAENQYWLS RPFLTAEQER GHAAVRRAAA FQALKAAQAA RFPAHRRLE EQLGHLLVTR KWS

UniProtKB: Mitochondrial ribosomal protein L57

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Macromolecule #36: mL62 (ICT1)

MacromoleculeName: mL62 (ICT1) / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 23.545873 KDa
SequenceString: MAAARCLRWG LNRAGAWLLP SPTRYPRRAL HKQVEGTEFQ SIYSLDKLYP ESRGSDTAWR LPDDAKQAND IPVDRLTISY CRSSGPGGQ NVNKVNSKAE VRFHLASADW IAEPVRLKLA VKHKNRINRS GELILTSECS RYQFRNLADC LQKLRDMIAE A SQPAKEPS ...String:
MAAARCLRWG LNRAGAWLLP SPTRYPRRAL HKQVEGTEFQ SIYSLDKLYP ESRGSDTAWR LPDDAKQAND IPVDRLTISY CRSSGPGGQ NVNKVNSKAE VRFHLASADW IAEPVRLKLA VKHKNRINRS GELILTSECS RYQFRNLADC LQKLRDMIAE A SQPAKEPS REDAELRRSR IENMNRERLR KKRISSAIKT SRRVDVD

UniProtKB: Large ribosomal subunit protein mL62

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Macromolecule #37: mL64

MacromoleculeName: mL64 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 25.815031 KDa
SequenceString: MAAPVQQART LLRLVTTLGQ GSRGYRARPP PRRSQEPRWP DPDDPLTPRW QLSPRYAAKQ FARHGAASGV AAGSLWPSQE QLRELEAEE REWYPSLAAM QESLRVQQLA EEQKRQAREQ LIEECMAKMP QMIENWRQQQ QERREKEQAD KDRRARLQAE A QERLGYHV ...String:
MAAPVQQART LLRLVTTLGQ GSRGYRARPP PRRSQEPRWP DPDDPLTPRW QLSPRYAAKQ FARHGAASGV AAGSLWPSQE QLRELEAEE REWYPSLAAM QESLRVQQLA EEQKRQAREQ LIEECMAKMP QMIENWRQQQ QERREKEQAD KDRRARLQAE A QERLGYHV DPRSARFQEL LQDMEKQHRK RLKEEKQRKK KEARAAAMAA AAAQDPADSE TPSS

UniProtKB: Large ribosomal subunit protein mL64

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Macromolecule #38: Mitochondrial ribosomal protein S18A

MacromoleculeName: Mitochondrial ribosomal protein S18A / type: protein_or_peptide / ID: 38 / Details: NCBI Reference Sequence: XP_005654252.2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 22.273084 KDa
SequenceString: MVGLNVLVSG CGRLLRGLLA GPAATSWARP PSRGFREVVE ILDGKTTIIE GRITETPQES PNPPNPTGQC PICRWNLKHK YNYEDVLLL SQFIRPHGGM LPRRITGLCQ EEHLKIEECV KMAHRAGLLP NHRPKLPEGF VPKSKPRLNR YLTRWSPRSV K PIYNKGHR ...String:
MVGLNVLVSG CGRLLRGLLA GPAATSWARP PSRGFREVVE ILDGKTTIIE GRITETPQES PNPPNPTGQC PICRWNLKHK YNYEDVLLL SQFIRPHGGM LPRRITGLCQ EEHLKIEECV KMAHRAGLLP NHRPKLPEGF VPKSKPRLNR YLTRWSPRSV K PIYNKGHR WNKVRMPVGS PLLKDNVSYS GRPLLLYH

UniProtKB: Mitochondrial ribosomal protein S18A

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Macromolecule #39: 39S ribosomal protein S30, mitochondrial

MacromoleculeName: 39S ribosomal protein S30, mitochondrial / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 49.100504 KDa
SequenceString: MAAARCWRFP LRGAGLSLHT AAKAAVTAPE VTGPDVPATP VARYPPIVAS LTAKSKAARQ RRVEQWQATV HAAKSVDEKL RILTKMQFM KYVVYPQTFA LNADNWYQSF TKTVFLSGLP PTPAKLEPEP TLDITALREA VCDCLLQEHF FLRRKKRAPV I QDREAIAS ...String:
MAAARCWRFP LRGAGLSLHT AAKAAVTAPE VTGPDVPATP VARYPPIVAS LTAKSKAARQ RRVEQWQATV HAAKSVDEKL RILTKMQFM KYVVYPQTFA LNADNWYQSF TKTVFLSGLP PTPAKLEPEP TLDITALREA VCDCLLQEHF FLRRKKRAPV I QDREAIAS PFLDQLVASL TGLLSVHNPV LAAAALDCKR PVHFFWLRGE EIIPRGHRKG RVDALRYQIN DKPHNQIRIS RQ LPEFVPL DYSIPIEVPV MSCKPDKLPL FKRQYENTIF IGSKTADPLC YGHTQFHLLP DKLKREKLLK QNCADQIEVV FRA NAIASL FAWTGAQAMY QGFWSEADVT RPFVSQGVIT DGKYFSFFCY QLNTLALTAQ ADQNNPRKNI CWGTQSKPLY ETIE DNNVK GFNDDVLLQL VQFLLNRPKE DKSQLLEN

UniProtKB: 39S ribosomal protein S30, mitochondrial

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Macromolecule #40: 39S ribosomal protein L1, mitochondrial

MacromoleculeName: 39S ribosomal protein L1, mitochondrial / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 34.372883 KDa
SequenceString: MVSQMPLYPR SVYIQLPNRH FAAAATKPAK KTQKGAKGKA SDEKKDDIEK IKSYPFMEGE PEDDVYLKRL YPRQIYEVEK AIHLLKKFQ VLDFTNPNQG VYLDLTLDMT LGKKKKVEPF ASVLSLPYPF TSEINKVAVF TGNASEIKIA EEKGAAFAGG T NLIQKILD ...String:
MVSQMPLYPR SVYIQLPNRH FAAAATKPAK KTQKGAKGKA SDEKKDDIEK IKSYPFMEGE PEDDVYLKRL YPRQIYEVEK AIHLLKKFQ VLDFTNPNQG VYLDLTLDMT LGKKKKVEPF ASVLSLPYPF TSEINKVAVF TGNASEIKIA EEKGAAFAGG T NLIQKILD DEIQPDFYVA VPEIMPELNP LRKKLKKRFP KLTRNSIGRD IPKMLELFKT GHEIKVDEER ENFLETKIAT LN MSSDQIA ANLQAVINEV CRQRPLNLGP FVVRAFLRSS TSEGLLIKIE PLLPKEVEAK ESGTLTA

UniProtKB: Large ribosomal subunit protein uL1m

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Macromolecule #41: Ribosomal protein

MacromoleculeName: Ribosomal protein / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 10.841016 KDa
SequenceString:
MATAFLRTVL SAVGPLLHLG GRPLSTFAAG PPRAALAVGA QPSPAAALLS ARPLLGPQPA LGFKTKGVLK KRCRDCYLVK RRGRWFIYC KTNPKHKQRQ M

UniProtKB: Ribosomal protein

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Macromolecule #42: Mitochondrial ribosomal protein L37

MacromoleculeName: Mitochondrial ribosomal protein L37 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 47.917988 KDa
SequenceString: MALASGPARR ALARLGRLGL GGCGAPRRGA YEWGVRSTRK PEPPPLDRVY EIPGLEPITY AGKMHFMPGL ARPVFPPWDP GWTHPKFRR LPPLHEHPLY KDQACYVFHQ RCRLLEGVKQ ALWLTKTQLI EGLPEKVLRL ADDPRNHIEN QDERVLNAIS H ARLWHSTE ...String:
MALASGPARR ALARLGRLGL GGCGAPRRGA YEWGVRSTRK PEPPPLDRVY EIPGLEPITY AGKMHFMPGL ARPVFPPWDP GWTHPKFRR LPPLHEHPLY KDQACYVFHQ RCRLLEGVKQ ALWLTKTQLI EGLPEKVLRL ADDPRNHIEN QDERVLNAIS H ARLWHSTE DIPKRETYCP VIVDSLIQLC KSQILKHPSL ARRICAQNNT LSATWNRESI LLQVHGSSGA RLNAKDPLPP VA SQEEVEA TKNHVLETFY PISPTMGLQE CNVYDVNDDT GFQEGYPYPC PHTLYFLESA NLRPRRFQPD QLRAKMILFA FGS ALAQAR LLYGNDSKVL EQPVVVQSVG TDGRLFQFLV LQLNTTDLAS DEGVKNLAWV DSDQLLYQHF WCLPVIKKKV VVEP VGPIG FQPETFRKFL ALYLHGAV

UniProtKB: Large ribosomal subunit protein mL37

+
Macromolecule #43: Mitochondrial ribosomal protein L38

MacromoleculeName: Mitochondrial ribosomal protein L38 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 44.656387 KDa
SequenceString: MAAPWWRAAL CASRRWRGFS TSAALSRRAA PLGPMPNEDI DVSDLERLKK YRSFDRYRRR AEQEARKPHW WRTYREHFGE ESGPKDRVD IGLPPPKVSR TQQLLERKQA LRELRANVEE ERAARLQTAR IPLEAVRAEW ERTCGPYHKQ RLAEYCGLYR D LFHGATFV ...String:
MAAPWWRAAL CASRRWRGFS TSAALSRRAA PLGPMPNEDI DVSDLERLKK YRSFDRYRRR AEQEARKPHW WRTYREHFGE ESGPKDRVD IGLPPPKVSR TQQLLERKQA LRELRANVEE ERAARLQTAR IPLEAVRAEW ERTCGPYHKQ RLAEYCGLYR D LFHGATFV PRVPLHVAYA VGEDDLMPVY HGNEVTPTEA AQAPEVTYEA DEGSLWTLLL TNLDGHLLEP DAEYVHWLVT NI PGNRVTE GQETCPYLPP FPARGSGFHR FAFLLFKQDK RIDFSGDTRP SPCYQLAQRT FHTFDFYKKH QDAMTPAGLA FFQ CRWDDS VTRVFHQLLD MREPVFEFVR PPPYHPKQKR FPHRQPLRYL DRYRDSHEPT YGIY

UniProtKB: Large ribosomal subunit protein mL38

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Macromolecule #44: Mitochondrial ribosomal protein L39

MacromoleculeName: Mitochondrial ribosomal protein L39 / type: protein_or_peptide / ID: 44 / Details: NCBI Reference Sequence: XP_003132793.4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 38.523422 KDa
SequenceString: MAMGAWGLRL WRAVPRGEAG WRLIATSPAS QLSPTELIEM QNDLFNKEKN RQLSLTPRTE KIEVKHVGKT DPGTVFVMNK NISTPYSCA MHLSEWYCRK SILALVDGQP WDMYKPLTKS CEIKFLTFKD DDPGEVNKAY WRSCAMMMGC VIERAFKDEY V VSLVRAPE ...String:
MAMGAWGLRL WRAVPRGEAG WRLIATSPAS QLSPTELIEM QNDLFNKEKN RQLSLTPRTE KIEVKHVGKT DPGTVFVMNK NISTPYSCA MHLSEWYCRK SILALVDGQP WDMYKPLTKS CEIKFLTFKD DDPGEVNKAY WRSCAMMMGC VIERAFKDEY V VSLVRAPE VPVIAGAFCY DVVLDKRLDE WMPTKENLHS FTKDARALIY KDLPFETLEV EAKVALEIFQ HNKYKLDFIE EK ASQNPER IVKLHRFGDF IDVSEGPLIP RTSICFQYEV SAVHNLQTQS SLVRRFQGLS LPVHLRAHFT IWNKLLERSR KMV TEDKTK PTEESAST

UniProtKB: Mitochondrial ribosomal protein L39

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Macromolecule #45: Mitochondrial ribosomal protein L40

MacromoleculeName: Mitochondrial ribosomal protein L40 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 19.406305 KDa
SequenceString:
MRAEPLRKKK KVDPKKDQAA KDRLKKRIRR LEKASQELIP IEDFITPVKF LNKERQRPPV ELPFEESERR ALLLKRWSLY KQREHEMER SAIRSLLEAQ EEALQELRLS SPELHAEATK RDPSLFPFER QGPDYTPPIS DYQPPEGRYQ DITKVYTQVE F KK

UniProtKB: Large ribosomal subunit protein mL40

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Macromolecule #46: Mitochondrial ribosomal protein L41

MacromoleculeName: Mitochondrial ribosomal protein L41 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 15.073404 KDa
SequenceString:
MGLLSGAARA LVRGADRMSK WTSKRGPRTF CKGRGAKGTG FHGRDGKFVQ IKEMIPELVV PELAGFKLKP YVNYRAPEGT DTPLTAKQL FLETAAPAIE KDFKAGTFDP EHLEKYGFEP TQEGKLFQLY PKNFPR

UniProtKB: Mitochondrial ribosomal protein L41

+
Macromolecule #47: mL42

MacromoleculeName: mL42 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 16.680178 KDa
SequenceString:
MALAAVKWVI SSRTILKHLF PIQNSASYCV CHKSTYSSLP DDYNCKVELA LTSDGRTIVC YHPSVDIPYE HTKPIPRPDP VHNIEETHD LVLKTRLEEK GEHLEQGPMI EQLSKMFFTT KHRWYPRGQY HRRRRKPNPP KDR

UniProtKB: Large ribosomal subunit protein mL42

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Macromolecule #48: 39S ribosomal protein L43, mitochondrial isoform X2

MacromoleculeName: 39S ribosomal protein L43, mitochondrial isoform X2 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 17.770146 KDa
SequenceString:
M(THC)ARGTASRF LTSVLHNGLG RYVQQLQRLS FSLSRDAPSS RGAREFVERE VTDFARRNPG VVIYVNPRPC CVPRVV AEY LNGAVREESI HCKSVEEIAA LVQKLADQSG LDVIRIRKPF HTDSPSIQGQ WHPFTNKPTT LGGLRPREVQ DPAPAQV QA Q

UniProtKB: Large ribosomal subunit protein mL43

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Macromolecule #49: mL44

MacromoleculeName: mL44 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 37.527195 KDa
SequenceString: MASGLVRLLQ WGPRRLLAPA APTLAPPVRG AKKGFRAAYR FQKELERWRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ETFSLDLLK TAFVNSCYIK SEEAKRQKLG IDKEAALLNL KDNQELSEQG ISFSQTCLTQ FFEDAFPDLP TEGVTSLVDF L TSEEVVCH ...String:
MASGLVRLLQ WGPRRLLAPA APTLAPPVRG AKKGFRAAYR FQKELERWRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ETFSLDLLK TAFVNSCYIK SEEAKRQKLG IDKEAALLNL KDNQELSEQG ISFSQTCLTQ FFEDAFPDLP TEGVTSLVDF L TSEEVVCH VARNLAVEQL ALSAEFPVPP PVLRQTFFAV IGALLQSSGP ERTALFIRDF LITQMTGKEL FEMWTITNPM GL LVEELKK RKISAPESRL TRQSGSTTAL PVYFVGLYCD RKLIAEGPGE TVLVAEEEAA RVALRKLFGF TENRRPWDYS KPK EHVRAE KTITAS

UniProtKB: Large ribosomal subunit protein mL44

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Macromolecule #50: Mitochondrial ribosomal protein L45

MacromoleculeName: Mitochondrial ribosomal protein L45 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 35.331805 KDa
SequenceString: MAAPVTRGLS CLPRVLGWWS RQPVLVTQST AVVPVRTKKR FTPPTYQPKY KSEKEFVEHA RKAGLVIPHE RLERPIHLAC TAGIFDAYV PPEGDARISS LSKEGLAQRA ERLKKNVASQ LSIRKIRESD PNFKIKDFPE KAKDIFIEAH LCLNNSDHDR L HTLVTENC ...String:
MAAPVTRGLS CLPRVLGWWS RQPVLVTQST AVVPVRTKKR FTPPTYQPKY KSEKEFVEHA RKAGLVIPHE RLERPIHLAC TAGIFDAYV PPEGDARISS LSKEGLAQRA ERLKKNVASQ LSIRKIRESD PNFKIKDFPE KAKDIFIEAH LCLNNSDHDR L HTLVTENC FPDMVWDIRY KTVRWSFVES LEPPQVVQVR CSSLMNQGNI YGQVTVRMHT RQTLAIYDRF GRLMYGQEDV PR DVLEYVV FEKHLVDPYG SWRMHGKIIP PWAPPKQPIL KTVMIPGPQL KPWEEFEEPQ GEVHKPQPA

UniProtKB: Large ribosomal subunit protein mL45

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Macromolecule #51: Mitochondrial ribosomal protein L46

MacromoleculeName: Mitochondrial ribosomal protein L46 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 32.215998 KDa
SequenceString: MAAPVRRTML RVVRGWRRFE GPWAHSLGSR NLALAVAPSS SSSPWRLLGA LCLQRPPLVT KPLTPLQEEM ADLLQQIEIE RSLYSDHEL RALDEAQQLA KKKSDLYEEE DEQNILLAQD LEDMWEQKFL HFKLGARLTE ADKKDDRTSL HRKLDRNLIL L VREKLGDQ ...String:
MAAPVRRTML RVVRGWRRFE GPWAHSLGSR NLALAVAPSS SSSPWRLLGA LCLQRPPLVT KPLTPLQEEM ADLLQQIEIE RSLYSDHEL RALDEAQQLA KKKSDLYEEE DEQNILLAQD LEDMWEQKFL HFKLGARLTE ADKKDDRTSL HRKLDRNLIL L VREKLGDQ DIWMLPQSDW QPGETLRQTA ERTLATLSEN NMEAKFLGNA PCGHYKFKFP QAMRTETSLG AKVFFFKALL LT GDFSQAG KKGHHVWVSK EELGDYLKPK YLAQVRRFLL DL

UniProtKB: Large ribosomal subunit protein mL46

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Macromolecule #52: 39S ribosomal protein L48, mitochondrial

MacromoleculeName: 39S ribosomal protein L48, mitochondrial / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 29.942523 KDa
SequenceString: MSEAAGTKVL RELNASRSDI VTGPDQGRSV SIFRPPGIAA AWADLQLARL HTTRQQRMNG ALGKALCLRN DTVLKQALSL IRVRASGES PICSAGGILL STSRHYRSKP THGIGRYKHL VKAQEPKKKK GKVEVRPINL GTDYEYGVLN IHLIAYDMAL A ESYAQYVH ...String:
MSEAAGTKVL RELNASRSDI VTGPDQGRSV SIFRPPGIAA AWADLQLARL HTTRQQRMNG ALGKALCLRN DTVLKQALSL IRVRASGES PICSAGGILL STSRHYRSKP THGIGRYKHL VKAQEPKKKK GKVEVRPINL GTDYEYGVLN IHLIAYDMAL A ESYAQYVH NLCNHLAIKV EESYAMPTKT MEVLQLQEQG SKMFLDAVLT THERVVQISG LSATFAEIFL EIIHSNLPEG VK LSVREHT EEDFKGRFKA RPELEELLAK LN

UniProtKB: 39S ribosomal protein L48, mitochondrial

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Macromolecule #53: 39S ribosomal protein L49, mitochondrial

MacromoleculeName: 39S ribosomal protein L49, mitochondrial / type: protein_or_peptide / ID: 53 / Details: NCBI Reference Sequence: NP_001231942.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 18.957779 KDa
SequenceString:
MAATVLCGVL RAWRTGVPLG CGLRRLSQTQ GTPEYPSFVE SVDEYHFVER LLPPASIPRP PKHEHYPTPS GWQPPRDPAP SLPYFVRRS RMHNIPVYRD ITHGNRQMTV IRKVEGDIWA LQKDVEDFLS PLLGKTPVTQ VNEVTGTLRV KGYFDQQLKA W LLEKGF

UniProtKB: 39S ribosomal protein L49, mitochondrial

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Macromolecule #54: Mitochondrial ribosomal protein L50

MacromoleculeName: Mitochondrial ribosomal protein L50 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 22.785357 KDa
SequenceString: MVRLRGKREC LTLSLRLIGW SWRVTAGGGR CFVSKFSSKM AARWVSGLAR RSLTCAVSGA PRREFWSPFR KEKQPVVAET VEEVKKEPI LVCPPIQSRT YIPPEDLQSR LESHVKEVFG SSVPSNWQDI SLEDVHLKFS FLARLANDLR HAVPNSRLHQ M CRVRDVLD ...String:
MVRLRGKREC LTLSLRLIGW SWRVTAGGGR CFVSKFSSKM AARWVSGLAR RSLTCAVSGA PRREFWSPFR KEKQPVVAET VEEVKKEPI LVCPPIQSRT YIPPEDLQSR LESHVKEVFG SSVPSNWQDI SLEDVHLKFS FLARLANDLR HAVPNSRLHQ M CRVRDVLD FYNVPVQDRS KFDELIASNL PHNLKITWGY

UniProtKB: Large ribosomal subunit protein mL50

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Macromolecule #55: Mitochondrial ribosomal protein L51

MacromoleculeName: Mitochondrial ribosomal protein L51 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 15.249048 KDa
SequenceString:
MAGSLSWVTG RGLWGRLPLT CRSFSLGIPR LFHVRVTLPP RKVVDRWNEK RAMFGVYDNI GILGNFEKHP KELIKGPIWL RGWKGNELQ RCIRKKRMVG NRMFIDDLHN LNKRISYLYK HFNRHGKYR

UniProtKB: Large ribosomal subunit protein mL51

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Macromolecule #57: Peptide chain release factor 1, mitochondrial

MacromoleculeName: Peptide chain release factor 1, mitochondrial / type: protein_or_peptide / ID: 57
Details: Human mitochondrial release factor 1 (mtRF1), with a C-terminal linker (GGSGGSG) and a 3xFLAG-tag (DYKDHDGDYKDHDIDYKDDDDK), expressed from a pcDNA3.1(+) plasmid in HEK293 EBNA cells.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 55.569742 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MNRHLCVWLF RHPSLNGYLQ CHIQLHSHQF RQIHLDTRLQ VFRQNRNCIL HLLSKNWSRR YCHQDTKMLW KHKALQKYME NLSKEYQTL EQCLQHIPVN EENRRSLNRR HAELAPLAAI YQEIQETEQA IEELESMCKS LNKQDEKQLQ ELALEERQTI D QKINMLYN ...String:
MNRHLCVWLF RHPSLNGYLQ CHIQLHSHQF RQIHLDTRLQ VFRQNRNCIL HLLSKNWSRR YCHQDTKMLW KHKALQKYME NLSKEYQTL EQCLQHIPVN EENRRSLNRR HAELAPLAAI YQEIQETEQA IEELESMCKS LNKQDEKQLQ ELALEERQTI D QKINMLYN ELFQSLVPKE KYDKNDVILE VTAGRTTGGD ICQQFTREIF DMYQNYSCYK HWQFELLNYT PADYGGLHHA AA RISGDGV YKHLKYEGGI HRVQRIPEVG LSSRMQRIHT GTMSVIVLPQ PDEVDVKLDP KDLRIDTFRA KGAGGQHVNK TDS AVRLVH IPTGLVVECQ QERSQIKNKE IAFRVLRARL YQQIIEKDKR QQQSARKLQV GTRAQSERIR TYNFTQDRVS DHRI AYEVR DIKEFLCGGK GLDQLIQRLL QSADEEAIAE LLDEHLKSAK GGSGGSGDYK DHDGDYKDHD IDYKDDDDK

UniProtKB: Peptide chain release factor 1, mitochondrial

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Macromolecule #2: E-site tRNA

MacromoleculeName: E-site tRNA / type: rna / ID: 2 / Details: likely the CCA-end of a tRNA / Number of copies: 1
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 894.612 Da
SequenceString:
CCA

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Macromolecule #3: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 3
Details: Corresponds to GenBank sequence ID NC_012095.1 (positions 1099-2669)
Number of copies: 1
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 504.908156 KDa
SequenceString: ACCAAAGCUA GCUCAACAUA CUAAACAAAU ACAAAAAUAC ACCAAAAUAA AAUAAAACAU UCACCUAACA UUAAAGUAUA GGAGAUAGA AAUUUUUAUC CUGACGCUAU AGAGAUAGUA CCGUAAGGGA AAGAUGAAAG AAUAAAAUAA AAGUAAAAAA A AGCAAAGA ...String:
ACCAAAGCUA GCUCAACAUA CUAAACAAAU ACAAAAAUAC ACCAAAAUAA AAUAAAACAU UCACCUAACA UUAAAGUAUA GGAGAUAGA AAUUUUUAUC CUGACGCUAU AGAGAUAGUA CCGUAAGGGA AAGAUGAAAG AAUAAAAUAA AAGUAAAAAA A AGCAAAGA UUACCCCUUC UACCUUUUGC AUAAUGGUUU AACCAGAAAA AAUCUAACAA AGAGAACUUU AGCUAGAUAC CC CGAAACC AGACGAGCUA CCCAUGAGCA GUUUAAAAGA ACCAACUCAU CUAUGUGGCA AAAUAGUGAG AAGACUUGUA GGU AGAGGU GAAAAGCCUA ACGAGCCUGG UGAUAGCUGG UUGUCCGAGA AAGAAUUUUA GUUCAACCUU AAAAAUACCC CAAA AACCC UAAAUUCCAA UGUAUUUUUA AGAGAUAGUC UAAAAAGGUA CAGCUUUUUA GAAACGGAUA CAACCUUGAC UAGAG AGUA AAAUCUUAAU ACUACCAUAG UAGGCCUAAA AGCAGCCAUC AAUUGAGAAA GCGUUAAAGC UCAACAAAUU CACCAA CAU AAUCCCAAAA ACUAAUAACA AACUCCUAGC CCAAUACCGG ACUAAUCUAU UGAAACAUAG AAGCAAUAAU GUUAAUA UG AGUAACAAGA AGCCUUUCUC CUCGCACACG CUUACAUCAG UAACUAAUAA UAUACUGAUA AUUAACAACC AAUAAACC A AAACAACACU AAAACGUUUA UUAAUUACAU UGUUAACCCA ACACAGGAGU GCACCAAGGA AAGAUUAAAA GAAGUAAAA GGAACUCGGC AAACACAAAC CCCGCCUGUU UACCAAAAAC AUCACCUCUA GCAUUACUAG UAUUAGAGGC AAUGCCUGCC CAGUGACAC CAGUUUAACG GCCGCGGUAU UCUGACCGUG CAAAGGUAGC AUAAUCACUU GUUCUCCAA(1MA) UAAGGAC UU GUAUGAAUGG CCACACGAGG GUUUUACUGU CUCUUACUUC CAAUCAGUGA AAUUAACCUU CCCGUGAAGA GGCGGGAA U AAAAAAAUAA GACGAGAAGA CCCUAUGGAG CUUUAAUUAA CUAUUCCAAA AGUUAAACAA CUCAACCACA AAGGGAUAA AACAUAACUU AACAUGGACU AGCAAUUUCG GUUG(OMG)GGUGA CCUCGGAGUA CAAAAAACCC UCCGAGUGAU UUUAAU CUA GACAAACCAG UCAAAAUAAC CAUAACAUCA CUUAUUGAUC CAAAAUUUUG AUCAACGGAA CAAGUUACCC UAGGGAU AA CAGCGCAAUC CUGUUCUAGA GUUCCUAUCG ACAAUAGGGU UUACGACCUC GAUGUUGGAU CAGGACACCC AAAUGGUG C AACCGCUAUU AAAGGUUCGU U(OMU)(OMG)UUCAACG AUUAAAGUCC UACGUGAUC(PSU) GAGUUCAGAC CGGAGCA AU CCAGGUCGGU UUCUAUCUAU UAUAAAUUUC UCCCAGUACG AAAGGACAAG AGAAAUGGGA CCAACCUCAC AAACGCGU C UCAGAGAUAA UUAAUGAUUU AAUCUUAACC UAAUUAACUC AUAAUAAAUC CAGCCCUAGA ACAGGGCACA

GENBANK: GENBANK: NC_012095.1

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Macromolecule #4: CP Phe-tRNA

MacromoleculeName: CP Phe-tRNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 23.516227 KDa
SequenceString:
GUUAAUGUAG CUUAAAUUAU CAAAGCAAGG CACUGAA(MIA)AU GCCUAGAUGA GCCUCACAGC UCCAUAAACA CCA

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Macromolecule #56: P-site Met-tRNA(fMet)

MacromoleculeName: P-site Met-tRNA(fMet) / type: rna / ID: 56
Details: Corresponds to GenBank sequence ID LC530718.1 (positions 1-71). In vitro transcribed human tRNA with formyl-methionine attached to the CCA-end of the tRNA.
Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.664498 KDa
SequenceString:
AGUAAGGUCA GCUAAAUAAG CUAUCGGGCC CAUACCCCGA AAAUGUUGGU UAUACCCUUC CCGUACUACC A

GENBANK: GENBANK: KY671085.1

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Macromolecule #58: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 58 / Number of copies: 205 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #59: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 59 / Number of copies: 30 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #60: PHENYLALANINE

MacromoleculeName: PHENYLALANINE / type: ligand / ID: 60 / Number of copies: 1 / Formula: PHE
Molecular weightTheoretical: 165.189 Da
Chemical component information

ChemComp-PHE:
PHENYLALANINE / Phenylalanine

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Macromolecule #61: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 61 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #62: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 62 / Number of copies: 1 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

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Macromolecule #63: N-FORMYLMETHIONINE

MacromoleculeName: N-FORMYLMETHIONINE / type: ligand / ID: 63 / Number of copies: 1 / Formula: FME
Molecular weightTheoretical: 177.221 Da
Chemical component information

ChemComp-FME:
N-FORMYLMETHIONINE / N-Formylmethionine

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Component:
ConcentrationFormulaName
20.0 mMC8H18N2O4SHEPES
100.0 mMKClpotassium chloride
20.0 mMMgCl2magnesium chloride

Details: 20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
GridModel: Quantifoil R2/2 / Material: COPPER / Support film - #0 - Film type ID: 1 / Support film - #0 - Material: CARBON / Support film - #0 - topology: HOLEY / Support film - #1 - Film type ID: 2 / Support film - #1 - Material: CARBON / Support film - #1 - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
DetailsIn vitro reconstituted complex (55S). Focused refinement on large subunit.

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 81000
Specialist opticsEnergy filter - Slit width: 20 eV
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 6973 / Average electron dose: 60.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1471587
Details: Particles were picked with blob picker in Cryosparc live v4.
Startup modelType of model: EMDB MAP
EMDB ID:

2914


Details: low-pass filtered to 40A
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4)
Details: Homogeneous refinement (Cryosparc v4) of all particles with EMD-2914 (low-pass filtered to 40A) as initial reference
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 50622
DetailsMicrographs were motion corrected and CTF parameters were estimated using Cryosparc live v4.
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial model
PDB IDChainDetails

7nqh

source_name: PDB, initial_model_type: experimental modelSus scrofa mitoribosome

7qi4

source_name: PDB, initial_model_type: experimental modelHigh-res mammalian mitoribosome

0-f1

source_name: AlphaFold, initial_model_type: in silico modelmtRF1 model prediction
RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-8oiq:
39S mammalian mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA

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