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- EMDB-16895: 28S mammalian mitochondrial small ribosomal subunit with mtRF1 an... -

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Basic information

Entry
Database: EMDB / ID: EMD-16895
Title28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA
Map data28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA
Sample
  • Complex: 28S mammalian mitochondrial small ribosomal subunit with mtRF1, mRNA, and P-site tRNA
    • Protein or peptide: x 33 types
    • RNA: x 3 types
  • Ligand: x 9 types
Keywordsmammalian mitochondrial ribosome / release factor mtRF1 / non-canonical stop codon / RIBOSOME
Function / homology
Function and homology information


Hormone ligand-binding receptors / gonadotropin-releasing hormone receptor binding / gonadotropin hormone-releasing hormone activity / G alpha (q) signalling events / Mitochondrial translation elongation / Mitochondrial translation termination / : / translation release factor activity / mitochondrial ribosome assembly / mitochondrial translational termination ...Hormone ligand-binding receptors / gonadotropin-releasing hormone receptor binding / gonadotropin hormone-releasing hormone activity / G alpha (q) signalling events / Mitochondrial translation elongation / Mitochondrial translation termination / : / translation release factor activity / mitochondrial ribosome assembly / mitochondrial translational termination / translation release factor activity, codon specific / Mitochondrial protein degradation / mitochondrial large ribosomal subunit / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation / organelle membrane / positive regulation of proteolysis / ribosomal small subunit binding / ribosomal small subunit assembly / small ribosomal subunit rRNA binding / regulation of translation / small ribosomal subunit / cytosolic small ribosomal subunit / nuclear membrane / cytosolic large ribosomal subunit / rRNA binding / ribosome / structural constituent of ribosome / mitochondrial matrix / ribonucleoprotein complex / translation / mRNA binding / apoptotic process / synapse / nucleolus / mitochondrion / extracellular space / RNA binding / nucleoplasm
Similarity search - Function
Gonadotropin-releasing hormone / Gonadoliberin / Gonadotropin-releasing hormone / Gonadotropin-releasing hormones signature. / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Cysteine alpha-hairpin motif superfamily / Peptide chain release factor / PCRF domain / PCRF / 28S ribosomal protein S27, mitochondrial ...Gonadotropin-releasing hormone / Gonadoliberin / Gonadotropin-releasing hormone / Gonadotropin-releasing hormones signature. / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Cysteine alpha-hairpin motif superfamily / Peptide chain release factor / PCRF domain / PCRF / 28S ribosomal protein S27, mitochondrial / MRPS27/PTCD2 / Mitochondrial 28S ribosomal protein S27 / Ribosomal protein S22, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / Mitochondrial 28S ribosomal protein S31 / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal protein S23 / Mitochondrial 28S ribosomal protein S31 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / 28S ribosomal protein S10, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / Peptide chain release factor class I superfamily / 28S ribosomal protein S24, mitochondrial / Mitochondrial 28S ribosomal protein S34 / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / 28S ribosomal protein S18b, mitochondrial / : / Mitochondrial ribosome subunit S24 / Mitochondrial 28S ribosomal protein S34 / Small ribosomal subunit protein uS5m, N-terminal / 28S ribosomal protein S25, mitochondrial / Prokaryotic-type class I peptide chain release factors signature. / Pentatricopeptide repeat domain / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L55 superfamily / Mitochondrial ribosomal protein L55 / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Pentatricopeptide (PPR) repeat profile. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Pentatricopeptide repeat / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Peptide chain release factor class I / RF-1 domain / CHCH / CHCH domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S15, bacterial-type / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S2 signature 1. / Ribosomal protein S2, conserved site / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S2 / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5 / Ribosomal protein S5, N-terminal / Ribosomal protein S5, N-terminal domain / Ribosomal protein S5, C-terminal / Ribosomal protein S5, C-terminal domain / Ribosomal protein S14 / Ribosomal protein S14p/S29e / Ribosomal S11, conserved site / Ribosomal protein S11 / Ribosomal protein S11 / Ribosomal protein S11 signature. / Ribosomal protein S10p/S20e / Ribosomal protein S10 domain
Similarity search - Domain/homology
Small ribosomal subunit protein uS12m / 28S ribosomal protein S14, mitochondrial / 28S ribosomal protein S18c, mitochondrial isoform 1 / Small ribosomal subunit protein mS25 / 28S ribosomal protein S28, mitochondrial / Small ribosomal subunit protein uS7m / Mitochondrial ribosomal protein S11 / 28S ribosomal protein S21, mitochondrial / Mitochondrial ribosomal protein S23 / Small ribosomal subunit protein uS15m ...Small ribosomal subunit protein uS12m / 28S ribosomal protein S14, mitochondrial / 28S ribosomal protein S18c, mitochondrial isoform 1 / Small ribosomal subunit protein mS25 / 28S ribosomal protein S28, mitochondrial / Small ribosomal subunit protein uS7m / Mitochondrial ribosomal protein S11 / 28S ribosomal protein S21, mitochondrial / Mitochondrial ribosomal protein S23 / Small ribosomal subunit protein uS15m / 28S ribosomal protein S24, mitochondrial / Mitochondrial ribosomal protein S22 / Mitochondrial ribosomal protein S34 / Progonadoliberin / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein bS16m / Mitochondrial ribosomal protein S6 / Mitochondrial ribosomal protein S27 / Mitochondrial ribosomal protein S17 / Small ribosomal subunit protein mS31 / Small ribosomal subunit protein uS10m / 28S ribosomal protein S2, mitochondrial / Small ribosomal subunit protein mS33 / 28S ribosomal protein S35, mitochondrial isoform 1 / Coiled-coil-helix-coiled-coil-helix domain containing 1 / Small ribosomal subunit protein uS5m / Mitochondrial ribosomal protein L55 / Large ribosomal subunit protein bL19m / Small ribosomal subunit protein mS29 / 28S ribosomal protein S9, mitochondrial / Small ribosomal subunit protein mS39 / Peptide chain release factor 1, mitochondrial / Small ribosomal subunit protein mS40
Similarity search - Component
Biological speciesSus scrofa (pig) / Homo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsSaurer M / Leibundgut M / Scaiola A / Schoenhut T / Ban N
Funding support Switzerland, European Union, 3 items
OrganizationGrant numberCountry
Swiss National Science Foundation179190 Switzerland
Swiss National Science Foundation182341 Switzerland
European Molecular Biology Organization (EMBO)1074-2019European Union
CitationJournal: Science / Year: 2023
Title: Molecular basis of translation termination at noncanonical stop codons in human mitochondria.
Authors: Martin Saurer / Marc Leibundgut / Hima Priyanka Nadimpalli / Alain Scaiola / Tanja Schönhut / Richard G Lee / Stefan J Siira / Oliver Rackham / René Dreos / Tea Lenarčič / Eva Kummer / ...Authors: Martin Saurer / Marc Leibundgut / Hima Priyanka Nadimpalli / Alain Scaiola / Tanja Schönhut / Richard G Lee / Stefan J Siira / Oliver Rackham / René Dreos / Tea Lenarčič / Eva Kummer / David Gatfield / Aleksandra Filipovska / Nenad Ban /
Abstract: The genetic code that specifies the identity of amino acids incorporated into proteins during protein synthesis is almost universally conserved. Mitochondrial genomes feature deviations from the ...The genetic code that specifies the identity of amino acids incorporated into proteins during protein synthesis is almost universally conserved. Mitochondrial genomes feature deviations from the standard genetic code, including the reassignment of two arginine codons to stop codons. The protein required for translation termination at these noncanonical stop codons to release the newly synthesized polypeptides is not currently known. In this study, we used gene editing and ribosomal profiling in combination with cryo-electron microscopy to establish that mitochondrial release factor 1 (mtRF1) detects noncanonical stop codons in human mitochondria by a previously unknown mechanism of codon recognition. We discovered that binding of mtRF1 to the decoding center of the ribosome stabilizes a highly unusual conformation in the messenger RNA in which the ribosomal RNA participates in specific recognition of the noncanonical stop codons.
History
DepositionMar 23, 2023-
Header (metadata) releaseMay 24, 2023-
Map releaseMay 24, 2023-
UpdateApr 24, 2024-
Current statusApr 24, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_16895.png

Downloads & links

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Map

FileDownload / File: emd_16895.map.gz / Format: CCP4 / Size: 476.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA
Voxel sizeX=Y=Z: 1.065 Å
Density
Contour LevelBy AUTHOR: 0.5
Minimum - Maximum-1.7491511 - 3.184897
Average (Standard dev.)-0.0009569857 (±0.102094635)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions500500500
Spacing500500500
CellA=B=C: 532.5 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: half map A

Fileemd_16895_half_map_1.map
Annotationhalf map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map B

Fileemd_16895_half_map_2.map
Annotationhalf map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 28S mammalian mitochondrial small ribosomal subunit with mtRF1, m...

EntireName: 28S mammalian mitochondrial small ribosomal subunit with mtRF1, mRNA, and P-site tRNA
Components
  • Complex: 28S mammalian mitochondrial small ribosomal subunit with mtRF1, mRNA, and P-site tRNA
    • Protein or peptide: 39S ribosomal protein L19, mitochondrial
    • Protein or peptide: bL31m
    • RNA: 12S rRNA
    • Protein or peptide: 28S ribosomal protein S35, mitochondrial isoform 1
    • Protein or peptide: Mitochondrial ribosomal protein S24
    • Protein or peptide: Aurora kinase A interacting protein 1
    • Protein or peptide: bS6m,Mitochondrial ribosomal protein S6
    • Protein or peptide: Mitochondrial ribosomal protein S7
    • RNA: P-site Met-tRNA(fMet)
    • Protein or peptide: Mitochondrial ribosomal protein S10
    • RNA: mRNA
    • Protein or peptide: Mitochondrial ribosomal protein S12
    • Protein or peptide: Mitochondrial ribosomal protein S14
    • Protein or peptide: 28S ribosomal protein S15, mitochondrial
    • Protein or peptide: 28S ribosomal protein S16, mitochondrial
    • Protein or peptide: uS17m
    • Protein or peptide: 28S ribosomal protein S18b, mitochondrial
    • Protein or peptide: Mitochondrial ribosomal protein S18C
    • Protein or peptide: 28S ribosomal protein S21, mitochondrial
    • Protein or peptide: Mitochondrial ribosomal protein S22
    • Protein or peptide: mS23
    • Protein or peptide: Mitochondrial ribosomal protein S25
    • Protein or peptide: Mitochondrial ribosomal protein S26
    • Protein or peptide: Mitochondrial ribosomal protein S27
    • Protein or peptide: Mitoribosomal protein ms28, mrps28
    • Protein or peptide: Death associated protein 3
    • Protein or peptide: 28S ribosomal protein S31, mitochondrial
    • Protein or peptide: Mitochondrial ribosomal protein S33
    • Protein or peptide: Peptide chain release factor 1, mitochondrial
    • Protein or peptide: Mitochondrial ribosomal protein S2
    • Protein or peptide: Coiled-coil-helix-coiled-coil-helix domain containing 1
    • Protein or peptide: 28S ribosomal protein S5, mitochondrial isoform X2
    • Protein or peptide: mS39
    • Protein or peptide: uS9m
    • Protein or peptide: Mitochondrial ribosomal protein S11
    • Protein or peptide: Mitochondrial ribosomal protein S34
  • Ligand: MAGNESIUM ION
  • Ligand: SPERMINE
  • Ligand: SPERMIDINE
  • Ligand: POTASSIUM ION
  • Ligand: ZINC ION
  • Ligand: FE-S-O HYBRID CLUSTER
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: GUANOSINE-5'-DIPHOSPHATE
  • Ligand: water

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Supramolecule #1: 28S mammalian mitochondrial small ribosomal subunit with mtRF1, m...

SupramoleculeName: 28S mammalian mitochondrial small ribosomal subunit with mtRF1, mRNA, and P-site tRNA
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#36
Details: Mammalian mitochondrial translation termination complex, in vitro reconstituted from porcine mitoribosomal subunits, human mtRF1-3xFLAG, human methionine-tRNA(formyl-methionine), and a ...Details: Mammalian mitochondrial translation termination complex, in vitro reconstituted from porcine mitoribosomal subunits, human mtRF1-3xFLAG, human methionine-tRNA(formyl-methionine), and a synthetic mRNA (5'-A AUG AGA CAA-3') containing the non-canonical stop codon AGA.
Source (natural)Organism: Sus scrofa (pig) / Tissue: Liver / Organelle: Mitochondria / Location in cell: Mitochondrial matrix
Molecular weightTheoretical: 1.1 MDa

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Macromolecule #1: 39S ribosomal protein L19, mitochondrial

MacromoleculeName: 39S ribosomal protein L19, mitochondrial / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 34.528809 KDa
SequenceString: MGVVVSTQRA GMAASITRGS WAAVGLGQSF PPTRALLPAP ASVICRTLAG SGRQQVTGSS EPGAFQPPPK PVIVDKRRPQ RPETRFLSP EFIPPRGRTN PLKFQIERKD MLERRKILHI PEFYVGSILR VTTADPYASA KTSQFLGICI QRSGSGLGAT F ILRNTIEG ...String:
MGVVVSTQRA GMAASITRGS WAAVGLGQSF PPTRALLPAP ASVICRTLAG SGRQQVTGSS EPGAFQPPPK PVIVDKRRPQ RPETRFLSP EFIPPRGRTN PLKFQIERKD MLERRKILHI PEFYVGSILR VTTADPYASA KTSQFLGICI QRSGSGLGAT F ILRNTIEG QGVEICFELY NPRIQEIQVV KLEKRLDNSL LYLRDALPEY STFDVNMKPI TQEFNQEVPV NQLKVKMKPK PW SKRWERP KFNIKGIRFD LCLTEEQMRE AQKWSQPWLE FDMMREYDTS KIEAAIWDEI EASKNS

UniProtKB: Large ribosomal subunit protein bL19m

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Macromolecule #2: bL31m

MacromoleculeName: bL31m / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 15.156537 KDa
SequenceString:
MAAKGSLLRL LWQRVVTGAA PESCRHLYTS SWRADCNRAL LTRLHRQTYA RLYPVLLVKQ DGSTIHIRYR EPRRMLTMPV DLDSLSPEE RRARFRKREA KFKEKKEEPE LSDDFDVEQY KRFWTKK

UniProtKB: Mitochondrial ribosomal protein L55

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Macromolecule #4: 28S ribosomal protein S35, mitochondrial isoform 1

MacromoleculeName: 28S ribosomal protein S35, mitochondrial isoform 1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 41.357789 KDa
SequenceString: MITVSCSRGG RARHVSLRSR ASGPPVDSLR ACPLPLAVCA DMAATSLPAW LILKSRAGTF RAFSTAMSPA TRSPRPALRT TERTSKPER ALRRKALPPR TEKMAVDQDW PSVYPVAAPF KPSAVPLPVR MGYPVKRGVP MAKEGNLELL KIPNFLHLTP V AIKRHCEA ...String:
MITVSCSRGG RARHVSLRSR ASGPPVDSLR ACPLPLAVCA DMAATSLPAW LILKSRAGTF RAFSTAMSPA TRSPRPALRT TERTSKPER ALRRKALPPR TEKMAVDQDW PSVYPVAAPF KPSAVPLPVR MGYPVKRGVP MAKEGNLELL KIPNFLHLTP V AIKRHCEA LKDFCTEWPA ALDSDEKCEK HFPIEIDTAD YVSAGPSIRN PKARVVTLRV KLSSLNLDDH AKKKLIKLVG DR YCKSTDV LTIKTDRCPL KRQNYDYAVY LLTVLYHESW KTEEWEKKKT EADMEEYIWE NSTSEKNILE TLLQIKAAEK NLE LSKEEL LGTKEVEDYR KSVVSLKNEG DNENTLSQYK ESVKRLLNLA

UniProtKB: 28S ribosomal protein S35, mitochondrial isoform 1

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Macromolecule #5: Mitochondrial ribosomal protein S24

MacromoleculeName: Mitochondrial ribosomal protein S24 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 18.935172 KDa
SequenceString:
MVASVCGGGL RPLVLSWSRE LPCAWRALHI SAVCAKNRAA RVRVSKGDKP VTYEEAHAPH YIAHRKGWLS LHTGNLDGED HAAERTVED VFLRKFMLGT FPGCLADQLV LKRRANQLEI CALVLRQLPP HKFYFLVGYS ETLLSHFYKC PVHLHLQTVP S KVVYKYI

UniProtKB: 28S ribosomal protein S24, mitochondrial

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Macromolecule #6: Aurora kinase A interacting protein 1

MacromoleculeName: Aurora kinase A interacting protein 1 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 22.843941 KDa
SequenceString: MFLVRLTSQL LRAVPGAGRS GSPRVSEVLG RHACRPRYST QPPGPSGIAS LPGKHVHPEL EEMLVPRKMS ISPLESWLTI RYLLPRLGA GTPGTVSPAQ LYQCPPSQVG EGVKQGDKEV RDTPQMQCKN ILKIRRRKMN HHKYRKLVKR TRFLRRKVRE G RLRQKQVK ...String:
MFLVRLTSQL LRAVPGAGRS GSPRVSEVLG RHACRPRYST QPPGPSGIAS LPGKHVHPEL EEMLVPRKMS ISPLESWLTI RYLLPRLGA GTPGTVSPAQ LYQCPPSQVG EGVKQGDKEV RDTPQMQCKN ILKIRRRKMN HHKYRKLVKR TRFLRRKVRE G RLRQKQVK FERDLKRIWQ KAGLKEAPAG WQTPKIYLKN Q

UniProtKB: Small ribosomal subunit protein mS38

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Macromolecule #7: bS6m,Mitochondrial ribosomal protein S6

MacromoleculeName: bS6m,Mitochondrial ribosomal protein S6 / type: protein_or_peptide / ID: 7
Details: NCBI Reference Sequence: XP_020925505.1,NCBI Reference Sequence: XP_020925505.1
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 41.085398 KDa
SequenceString: MGYDPSPVAH SLRLTPTTLG KGPQEAALAA ALEHRAVGRG GRGSTPRVYP AGAARGRRPK RERSGTRMRG QSRGKGARTD QTAPQRTRT RGRTLARSSP RMRRKAWKVP AGSRTIRSKR GGSGSCRVGG VCSSRWQLAE GTRGAALLQW LPGQGAGPRA G CRPQRAGL ...String:
MGYDPSPVAH SLRLTPTTLG KGPQEAALAA ALEHRAVGRG GRGSTPRVYP AGAARGRRPK RERSGTRMRG QSRGKGARTD QTAPQRTRT RGRTLARSSP RMRRKAWKVP AGSRTIRSKR GGSGSCRVGG VCSSRWQLAE GTRGAALLQW LPGQGAGPRA G CRPQRAGL SRGRGAAALR RGRGVARGGA WLGEGAWRPV CAAARAFKPG LARPERAFAA WEPSGPAVRQ VSAVPALFHP GR RFREEPR AVHSGMPRYE LALILKAMQR PETAAALKRT LEALMDRGAV VRNLENLGER MLPYKISAHN QRHSRGGYFL VDF YAPATT VESMMEHLSR DIDVIRPNIV KHPLTQEVKE CEGIVPVPLE EKLYSTKKRK

UniProtKB: Mitochondrial ribosomal protein S6

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Macromolecule #8: Mitochondrial ribosomal protein S7

MacromoleculeName: Mitochondrial ribosomal protein S7 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 28.150918 KDa
SequenceString: MAAPAVKAAR GWSCSALGVR IAVLRFPGLT QVRWSRYGPE YKDPQIDKEY YRKPLAEQTE EEKYERDFKK TQLIKAAPAT KTSSVFEDP VISKFTNMMM KGGNKVLARS LMTQTLEAVK RKQFAKYHAA SAEEQATIER NPYTIFHQAL KNCEPVIGLV P ILKGGHFY ...String:
MAAPAVKAAR GWSCSALGVR IAVLRFPGLT QVRWSRYGPE YKDPQIDKEY YRKPLAEQTE EEKYERDFKK TQLIKAAPAT KTSSVFEDP VISKFTNMMM KGGNKVLARS LMTQTLEAVK RKQFAKYHAA SAEEQATIER NPYTIFHQAL KNCEPVIGLV P ILKGGHFY QVPVPLADRR RRFLAMKWMI AECREKKHRR VLMPEKLSQE LLEAFHNQGP VIKRKHDMHK MAEANRALAH YR WW

UniProtKB: Small ribosomal subunit protein uS7m

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Macromolecule #10: Mitochondrial ribosomal protein S10

MacromoleculeName: Mitochondrial ribosomal protein S10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 23.105678 KDa
SequenceString: MAARTAFGAL ARRLWQGSRN FSVSSSRSST AKNGGFLLTT MKWVQFSNLH VDVPKDMTKP EITISDEPDT LYKRLSVLVK GHDKAVLDS YEYFAVLAAK ELGISIKVHE PPRKIERFTL LKSVHIFKKH RVQYEMRTLY RCLELEHLTG STADVYLEYI Q RNLPEGVA ...String:
MAARTAFGAL ARRLWQGSRN FSVSSSRSST AKNGGFLLTT MKWVQFSNLH VDVPKDMTKP EITISDEPDT LYKRLSVLVK GHDKAVLDS YEYFAVLAAK ELGISIKVHE PPRKIERFTL LKSVHIFKKH RVQYEMRTLY RCLELEHLTG STADVYLEYI Q RNLPEGVA MEVTKTKLEQ LPEHIRKPIW ETMPEEKEES KS

UniProtKB: Small ribosomal subunit protein uS10m

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Macromolecule #12: Mitochondrial ribosomal protein S12

MacromoleculeName: Mitochondrial ribosomal protein S12 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 15.257107 KDa
SequenceString:
MSWSGLLRGL GTSLNCGLAL VPRPWATRPM ATLNQMHRLG PPKHPPGKMG PTAGRPQLKG VVLRTFIRKP KKPNSANRKC CRVRLSTGR EAVCFIPGEG HSLQEHHVVL VQGGRTQDLP GVKLTVVRGK YDCGHVQKKK

UniProtKB: Small ribosomal subunit protein uS12m

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Macromolecule #13: Mitochondrial ribosomal protein S14

MacromoleculeName: Mitochondrial ribosomal protein S14 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 15.120739 KDa
SequenceString:
MAASMLGFLL RTVRQMVPSS ASGQVRSYYV DWKMLRDVKR RKMAYEYADE RLRINSLRKN TILPKDLQEV ADEEIASLPR DSCPVRIRN RCVMTSRPRG VKRRWRLSRI VFRHLADHGQ LSGVQRAMW

UniProtKB: 28S ribosomal protein S14, mitochondrial

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Macromolecule #14: 28S ribosomal protein S15, mitochondrial

MacromoleculeName: 28S ribosomal protein S15, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 29.596867 KDa
SequenceString: MLRAAWRALS SIRTQAVTQA PVLGLPGGGC ARFPSVQRAL PSRPAGLILR AARGYAAQKP GEEQGLTGED RLISHCLCLN IQPNQDDPP PSMLLLDYQN VPGIHKVDDV VKRLLSLEMA NQKEKLKIKK MQLMNKVLEN PEDTSSLEAR IVALTVKIRN Y EEHMQKHR ...String:
MLRAAWRALS SIRTQAVTQA PVLGLPGGGC ARFPSVQRAL PSRPAGLILR AARGYAAQKP GEEQGLTGED RLISHCLCLN IQPNQDDPP PSMLLLDYQN VPGIHKVDDV VKRLLSLEMA NQKEKLKIKK MQLMNKVLEN PEDTSSLEAR IVALTVKIRN Y EEHMQKHR KDKAHKRFLL MSIDQRKKML KNLRETNYAV FEKICKELGI EYTFPPPYHR KAHRRWVTKK ALCTQVFREV QK LKKQKRA LRAAAVAAHK QG

UniProtKB: Small ribosomal subunit protein uS15m

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Macromolecule #15: 28S ribosomal protein S16, mitochondrial

MacromoleculeName: 28S ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 15.182668 KDa
SequenceString:
MVQLTTVLCR AYRGGHLTIR LALGGCTNRP FYRIVAAHSK CPRDGRFVEQ LGSYDPLPNS HGEKLVALNF DRIRHWIGCG AHLSKPVEK LLGLSGFYPL HPMMITNAER LRRKRAREVL LASQKTDTEA TETKAS

UniProtKB: Small ribosomal subunit protein bS16m

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Macromolecule #16: uS17m

MacromoleculeName: uS17m / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 14.452014 KDa
SequenceString:
MSIARSSVHA KWVVGKVIGT AMQKTAKVRV TRLVLDPYLL KYFNKRKTYF AHDALQQCTV GDIVLLKALP VPRTKHVKHE LAEIIFKVG QVIDPVTGKP CAGTTYLESP ISPESTRLTK NLEALQLSST Q

UniProtKB: Mitochondrial ribosomal protein S17

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Macromolecule #17: 28S ribosomal protein S18b, mitochondrial

MacromoleculeName: 28S ribosomal protein S18b, mitochondrial / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 29.220465 KDa
SequenceString: MAASILNVLL RRLPGVSPFR GAYGVQVLLQ TLCTKAPPED DSLPQVPISP YKDEPWKYLD SEEYQNRYGS RPVWADYRRN HKGGIPPQR TRKMCIRGNK VAGNPCPICR DQKLHVDFRN VKLLEQFVCA HTGIIFHAPY TGVCMKQHKK LTQAIQKARD H GLLRYHIP ...String:
MAASILNVLL RRLPGVSPFR GAYGVQVLLQ TLCTKAPPED DSLPQVPISP YKDEPWKYLD SEEYQNRYGS RPVWADYRRN HKGGIPPQR TRKMCIRGNK VAGNPCPICR DQKLHVDFRN VKLLEQFVCA HTGIIFHAPY TGVCMKQHKK LTQAIQKARD H GLLRYHIP QVEPRDLDFS TTHGAVSSTP PAPTLVSGDP WYPWYSWKQP PERELSRLRR LYQGRLREES GPPPELMPEV PL TAPAEAS STEPGAPQSA L

UniProtKB: Small ribosomal subunit protein mS40

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Macromolecule #18: Mitochondrial ribosomal protein S18C

MacromoleculeName: Mitochondrial ribosomal protein S18C / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 16.211086 KDa
SequenceString:
MAAVVSLCSR LGRRKSTDFV NAAVCLTDPG THAVLWRSCS QYKQVASNED LPVPMENPYK EPLKKCILCE KHVDYKNVQL LSQFISPFT GCIYGRHITG LCGKKQKEIT KAIKRAQILG FMPVTYKDPA YLKDPKVCNI KYRE

UniProtKB: 28S ribosomal protein S18c, mitochondrial isoform 1

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Macromolecule #19: 28S ribosomal protein S21, mitochondrial

MacromoleculeName: 28S ribosomal protein S21, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 10.724543 KDa
SequenceString:
M(AYA)NHLKFIAR TVMVQEGNVE GAYRTLNRIL TMDGLIEDIK RRRYYEKPCR RRQRESYETC RRIYNMEMAR KINFLM RKN RADPWQGC

UniProtKB: 28S ribosomal protein S21, mitochondrial

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Macromolecule #20: Mitochondrial ribosomal protein S22

MacromoleculeName: Mitochondrial ribosomal protein S22 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 43.234645 KDa
SequenceString: MPHEPRETRN LSLPLPALVA RIIMATLKAS ALLRSLQTNS CGTGRVCFPV RARPRPRALL QPLPGASGTG TLCRGLGSES ESGNSEIRK PTFMDEEVQN ILIKMTGLDL QKIFKPALQE LKPPTYKLMT QAQLEEATKQ AVEAAKVRLK MPPVLEERAP I NDVLAEDK ...String:
MPHEPRETRN LSLPLPALVA RIIMATLKAS ALLRSLQTNS CGTGRVCFPV RARPRPRALL QPLPGASGTG TLCRGLGSES ESGNSEIRK PTFMDEEVQN ILIKMTGLDL QKIFKPALQE LKPPTYKLMT QAQLEEATKQ AVEAAKVRLK MPPVLEERAP I NDVLAEDK ILEGTETAKY VFTDISYSIP HRERFIVVRE PSGTLRKASW EERDRMIQVY FPREGRRILT PVIFKEENLQ TM YSQDQHV DVLNLCVAQF EPDSAEYIKI HHHTYEDIDK CGKYDLLRST RHFGGMAWYF VNKKKIDGLL IDQIQRDLVS DAT SLVHLY HILHPDGQSA QEAKKQGAEG LHLIKVFAKT EAQKGAYIEL TLQAYQEAFI THSAAS

UniProtKB: Mitochondrial ribosomal protein S22

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Macromolecule #21: mS23

MacromoleculeName: mS23 / type: protein_or_peptide / ID: 21 / Details: NCBI Reference Sequence: XP_005669023.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 21.666674 KDa
SequenceString:
MAGSRLETVG SIFSRTRDLI RAGVLKEKPL WLDIYNAFPP LREPVFRRPR LRYGKAKAAV QDIFYHEDRI RAKFYSAYGS GPKAFDLFN PNFKSTCQRF VEKYIELQRL GETDEEKLFV EAGKALLAEG VTLRRVGEAR TQQEGSPISR KSEPTGIKPQ T AVEENQPW KEVPQDQPLE APEKESKGLP PP

UniProtKB: Mitochondrial ribosomal protein S23

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Macromolecule #22: Mitochondrial ribosomal protein S25

MacromoleculeName: Mitochondrial ribosomal protein S25 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 19.954186 KDa
SequenceString:
MPMKGRFPIR RTLQYLGQGD VVFKDSVKVM TVNYNTHGEL GEGARKFVFF NIPQIQYKNP WVQITMFKNM TPSPFLRFYL DSGEQVLVD VESKSNKEIV EHIRKILGKN EETLEKEEQE KKQLSHPAHF GPRKYCLREC MCEVEGQVPC PGLVPLPKEM T GKYKAALK ATAQD

UniProtKB: Small ribosomal subunit protein mS25

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Macromolecule #23: Mitochondrial ribosomal protein S26

MacromoleculeName: Mitochondrial ribosomal protein S26 / type: protein_or_peptide / ID: 23 / Details: NCBI Reference Sequence: XP_001924843.2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 23.6379 KDa
SequenceString: MLRVLGGLGA RPAGRLPAPL LLPTRGRKTR HDPPAKSKAG RVATPPAVDP TEFFVLTERY RQYRQTVRAL RLEFMSEVRK KLHEARAGV QAERKAQEDA AEHRELMAWN QAENQRLHEL RLARLRQEAL EQERRQAEEA VLQAREAQAW AQLKEQEVLQ L QEEAKTFI ...String:
MLRVLGGLGA RPAGRLPAPL LLPTRGRKTR HDPPAKSKAG RVATPPAVDP TEFFVLTERY RQYRQTVRAL RLEFMSEVRK KLHEARAGV QAERKAQEDA AEHRELMAWN QAENQRLHEL RLARLRQEAL EQERRQAEEA VLQAREAQAW AQLKEQEVLQ L QEEAKTFI TRENLEARVE EALDSPKSYN WAITREGLVV RPQQKGS

UniProtKB: Progonadoliberin

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Macromolecule #24: Mitochondrial ribosomal protein S27

MacromoleculeName: Mitochondrial ribosomal protein S27 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 45.761789 KDa
SequenceString: VVKSCRRCLL SAAYVDSHKW EARDKEDCHL ADLASLMDKT YERKLPVSSL TISRFVDNIA SREDIDHAEY YLYKFRHSPN CWYLRDWTI HTWIRQCLKY GAQDKALYTL KNKVQYGIFP DNYTFNLLMD YFIKKENYKD ALSVVFEIMM QEAFEVPSTQ L LSLYVLYH ...String:
VVKSCRRCLL SAAYVDSHKW EARDKEDCHL ADLASLMDKT YERKLPVSSL TISRFVDNIA SREDIDHAEY YLYKFRHSPN CWYLRDWTI HTWIRQCLKY GAQDKALYTL KNKVQYGIFP DNYTFNLLMD YFIKKENYKD ALSVVFEIMM QEAFEVPSTQ L LSLYVLYH CLAEKTDLSW EEERNFGASL LLPGLKQKNS VGLSSQLYGY ALLGKVELQQ GLRAVYCNMP LLWQPGYLHR AL QVMEKVA SSPEDGQLCG EALDVLDRVL KAVTAPAQGT SEEQPQEGEE SQRSEELVEQ LDVEETEQSK LPQYLERFEA LRS KLQALG KVESESLLTL STQLAKAQLP TCEAEDIAVY EQRLQQWHGE RVRLIQREQE QRERARQDYE ARRAAKASA

UniProtKB: Mitochondrial ribosomal protein S27

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Macromolecule #25: Mitoribosomal protein ms28, mrps28

MacromoleculeName: Mitoribosomal protein ms28, mrps28 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 21.014912 KDa
SequenceString:
MAALCRTRAV TAKSHFLRVF FFSRPCRGTG TESGSGSESS ESTEPKQRPG GFASALERHS ELQQKAEFGR MRGSPKNVES FASMLRHSP LTQMGPAKNK LVIGQIFHIV EDDLYIDFGG KFHCVCKRPE VDGEKYQKGT RVRLRLLDLE LTSRFLGATT D TTILEADA VLLGLQESKD SKSKEERHEK

UniProtKB: 28S ribosomal protein S28, mitochondrial

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Macromolecule #26: Death associated protein 3

MacromoleculeName: Death associated protein 3 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 46.966062 KDa
SequenceString: MAQLLANLTS IQVMLKGVTR LISRVHKLDP GHVLYMGTQA PQSLAAHLDN QVPVKSPRAI SRTSEDDPAK HREQHEGQHY NISLQELKT VFPHGLPPRF AMQVKTFNEA CLMVRKPALE LLHYLKNTNF AHPAVRYVLY GEKGTGKTLS LCHILHFCAK Q NWLILHIP ...String:
MAQLLANLTS IQVMLKGVTR LISRVHKLDP GHVLYMGTQA PQSLAAHLDN QVPVKSPRAI SRTSEDDPAK HREQHEGQHY NISLQELKT VFPHGLPPRF AMQVKTFNEA CLMVRKPALE LLHYLKNTNF AHPAVRYVLY GEKGTGKTLS LCHILHFCAK Q NWLILHIP DAHIWVKNCR DLLQSNYNKQ RFDQPLEAST WLKNFKTANE HFLSQIKVQE KYVWNKREST EKGRPLGEVV EQ GIMRVRN ATDAVGIVLK ELKRQSSLGI FHLLVAVDGV NALWGRTTLK REDKSPIAPE ELALIHNLRK MVKNDWQGGA IVL TVSQTG SLFKPRNAYL PQELLGKEGF DALDPFIPIL VSNYNPKEFE SCIQYYLENN WLQHEKAHTE EGKKELLFLS NRNP GQLER LCAYL

UniProtKB: Small ribosomal subunit protein mS29

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Macromolecule #27: 28S ribosomal protein S31, mitochondrial

MacromoleculeName: 28S ribosomal protein S31, mitochondrial / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 43.516496 KDa
SequenceString: MLPRVSAVIP LRPLSRLPLC SGGPEASAAA ILLLDSLRGT VRTENNIQRY FGTNNLVYSK KDEQSGPTCE ISKETESKGS VKENSKKDL LNIIKGMKVE LSTVNVQTTK PSNKRQLKNL ETAIGRIKKA PEDTPQKRSE SLSSELVAAV SAVADSLPFD R RMTKSELL ...String:
MLPRVSAVIP LRPLSRLPLC SGGPEASAAA ILLLDSLRGT VRTENNIQRY FGTNNLVYSK KDEQSGPTCE ISKETESKGS VKENSKKDL LNIIKGMKVE LSTVNVQTTK PSNKRQLKNL ETAIGRIKKA PEDTPQKRSE SLSSELVAAV SAVADSLPFD R RMTKSELL RQLQKHEEDS RDKKKKLIFS NIISDMKVAR SATARVSTRP VHQIQFDEGT DDFADQEKTL NLKKRKNIFK GR RLNIFEP QAVTEEAPET EAVPSLWDIE FAKQLAAVTE RPFQNGFEEM IQWTKEGKLW EFPINNEAGF DDDGSEFHEH IFL DKYLQD FPKQGPIRHF MELVTCGLSK NPYLSVKQKV EHIEWFRNYF NEKRVILKES GIQLN

UniProtKB: Small ribosomal subunit protein mS31

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Macromolecule #28: Mitochondrial ribosomal protein S33

MacromoleculeName: Mitochondrial ribosomal protein S33 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 16.971662 KDa
SequenceString:
MRDGFRAPAE VSSLVESPRI GRINFINQLG GLTGTLAPAA QEMSSLSEYA VRMSRLSARL FGEVARPTDS KSMKVVKLFS EQPLAKRKE TYDWYPNHNT YFALMGTLRF LGLYRDEHQD FRDEQLRLKK LRGKGKPRKG EGKRAMKKK

UniProtKB: Small ribosomal subunit protein mS33

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Macromolecule #29: Peptide chain release factor 1, mitochondrial

MacromoleculeName: Peptide chain release factor 1, mitochondrial / type: protein_or_peptide / ID: 29
Details: Human mitochondrial release factor 1 (mtRF1), with a C-terminal linker (GGSGGSG) and a 3xFLAG-tag (DYKDHDGDYKDHDIDYKDDDDK), expressed from a pcDNA3.1(+) plasmid in HEK293 EBNA cells.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 55.569742 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MNRHLCVWLF RHPSLNGYLQ CHIQLHSHQF RQIHLDTRLQ VFRQNRNCIL HLLSKNWSRR YCHQDTKMLW KHKALQKYME NLSKEYQTL EQCLQHIPVN EENRRSLNRR HAELAPLAAI YQEIQETEQA IEELESMCKS LNKQDEKQLQ ELALEERQTI D QKINMLYN ...String:
MNRHLCVWLF RHPSLNGYLQ CHIQLHSHQF RQIHLDTRLQ VFRQNRNCIL HLLSKNWSRR YCHQDTKMLW KHKALQKYME NLSKEYQTL EQCLQHIPVN EENRRSLNRR HAELAPLAAI YQEIQETEQA IEELESMCKS LNKQDEKQLQ ELALEERQTI D QKINMLYN ELFQSLVPKE KYDKNDVILE VTAGRTTGGD ICQQFTREIF DMYQNYSCYK HWQFELLNYT PADYGGLHHA AA RISGDGV YKHLKYEGGI HRVQRIPEVG LSSRMQRIHT GTMSVIVLPQ PDEVDVKLDP KDLRIDTFRA KGAGGQHVNK TDS AVRLVH IPTGLVVECQ QERSQIKNKE IAFRVLRARL YQQIIEKDKR QQQSARKLQV GTRAQSERIR TYNFTQDRVS DHRI AYEVR DIKEFLCGGK GLDQLIQRLL QSADEEAIAE LLDEHLKSAK GGSGGSGDYK DHDGDYKDHD IDYKDDDDK

UniProtKB: Peptide chain release factor 1, mitochondrial

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Macromolecule #30: Mitochondrial ribosomal protein S2

MacromoleculeName: Mitochondrial ribosomal protein S2 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 31.731604 KDa
SequenceString: MASGPLLPRL LGAGAPRRPL RLHLVPTATP GPAGPTGRTL GSNAAPAARE PELGNDLRDR ILSEPLKHAD FFNLKELFSV RSLFDARVH LGHKAGCRHR FMEPYLFGSR LGQDIIDLEQ TAAHLQLALN FTAHVAYREG IILFVSRHRQ FAHLIETTAR D CGEYAHTR ...String:
MASGPLLPRL LGAGAPRRPL RLHLVPTATP GPAGPTGRTL GSNAAPAARE PELGNDLRDR ILSEPLKHAD FFNLKELFSV RSLFDARVH LGHKAGCRHR FMEPYLFGSR LGQDIIDLEQ TAAHLQLALN FTAHVAYREG IILFVSRHRQ FAHLIETTAR D CGEYAHTR YFKGGLLTNA PLLLGPGVRL PDLIIFLHTL NNVFEPHVAV RDAAKMNIPT VGIVDTNCNP ALITYPVPGN DD SPPAVRL FCRLFQVAIS RAKEKRRQVE ALYRLQGPAG AERGPAAPPP PE

UniProtKB: 28S ribosomal protein S2, mitochondrial

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Macromolecule #31: Coiled-coil-helix-coiled-coil-helix domain containing 1

MacromoleculeName: Coiled-coil-helix-coiled-coil-helix domain containing 1
type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 13.603939 KDa
SequenceString:
M(AYA)TPSLRGRL ARLGNPRRPI LKPNKPLILA NHVGERRREK GEATCITEMS VMMACWKQNE FRDEACRKEI QDFFDC ASR AEAARKMRSI QENLGELGSL PPKKLNKLLH RFPNKPHVS

UniProtKB: Coiled-coil-helix-coiled-coil-helix domain containing 1

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Macromolecule #32: 28S ribosomal protein S5, mitochondrial isoform X2

MacromoleculeName: 28S ribosomal protein S5, mitochondrial isoform X2 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 48.213074 KDa
SequenceString: MAAAVRAAGF FPGLFGASAG NLWSRQLYLN TFPAASILTS KTFPSNGPLS PPGTRDNHHF TSLACALQTQ CSISSPSNWM GQQYRSYSF FTKLTADELW KGALAESGAG ARKGRGKRTK KKRRKDLNRG QIIGEGRHGF LWPGLNIPLM RNGAVQTIAQ R SKEDQEKV ...String:
MAAAVRAAGF FPGLFGASAG NLWSRQLYLN TFPAASILTS KTFPSNGPLS PPGTRDNHHF TSLACALQTQ CSISSPSNWM GQQYRSYSF FTKLTADELW KGALAESGAG ARKGRGKRTK KKRRKDLNRG QIIGEGRHGF LWPGLNIPLM RNGAVQTIAQ R SKEDQEKV EADMVQQREE WDRRRKMKVK RERGWSGNTW GGVSLGPPDP GPNGETYDDF DTRILEVRNV FNMTAKEGRK RS VRVLVAV GNGKGAAGFA IGKATERADA FRKAKNRAVH YLHYIERYED HTIYHDISLK FKRTHIKMKK QPRGYGLHCH RAI MTICRL IGIKDLYAKV SGSVNMLNLT RGLFLGLSRQ ETHQQLADKK SLHVVEFREE CGPLPIVVAS PQGALRKDPE PEDE VPDIT LDWEDVKAAQ GMKRSVWSGL KRAAT

UniProtKB: Small ribosomal subunit protein uS5m

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Macromolecule #33: mS39

MacromoleculeName: mS39 / type: protein_or_peptide / ID: 33 / Details: NCBI Reference Sequence: XP_005662461.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 78.518156 KDa
SequenceString: MAAVASARWL GVRSGLCLPL TGRRVGPCGR TPRSRFYSGS AAHPEVEGAN VTGIEEVVIP KKKTWDKVAI LQALASTVHR DSTAAPYVF QDDPYLIPTS SVESHSFLLA KKSGENAAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI EDVSEAALQE R IKLRKVKA ...String:
MAAVASARWL GVRSGLCLPL TGRRVGPCGR TPRSRFYSGS AAHPEVEGAN VTGIEEVVIP KKKTWDKVAI LQALASTVHR DSTAAPYVF QDDPYLIPTS SVESHSFLLA KKSGENAAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI EDVSEAALQE R IKLRKVKA SVDMFDQLLQ AGTTVSLETT NSLLDLLCYY GNQEPSANYN FQQREQSEEL EEATEADNEK SKTKAGHHLG VT WRTKNNA ERIFALMPEK NAHSYCTMIR GMVKHQAPTQ ALNLYTVLLN NRLRADVYTF NSLIEATALV VNEKFEEKWN NIL DLLKQM VTQNVKPNLQ TFNTILKCLR RFYAFGKLPA LQTLREMKAI GIEPSLATYH YVIQLFYQHE SPSKGSSLII YDIM NEVMG KRFSPRDPDD DMFFQSAMRV CSSLRDLELA YQVHGLLNTG DNWKLIGSDH RRNFYYSKFF NLLCFMEQID VTLKW YKDL IPSVFFPHSQ TMIDLLQALD VANRLDMVPQ IWKDSKEYGH TFRNELKEEI LMLMARDQHP PELQVAFADC AADIKS TYE SQDARQTAPE WPASSLNYVA VLFLRAGRTQ EAWKMLGLFR KHNKIPRAEL LNEFLDSAKA SSSPAQAIEL VKLASAF SL PVCEGLTRRV MAEFTLTQEQ REALGELTAL TSDSSSDSDS DSDSDISEGK

UniProtKB: Small ribosomal subunit protein mS39

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Macromolecule #34: uS9m

MacromoleculeName: uS9m / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 45.60457 KDa
SequenceString: MAAPCVFCGA AVSYRLFLGC RVSFARGQVL WKAAAPELQA GARCQILRLR HTAVVTTKKN VAASRRETYT VDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPIMKHPE EIFPKQRAIQ WGEDGRPFHF LFYTGKQSYY S LMHDTYGK ...String:
MAAPCVFCGA AVSYRLFLGC RVSFARGQVL WKAAAPELQA GARCQILRLR HTAVVTTKKN VAASRRETYT VDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPIMKHPE EIFPKQRAIQ WGEDGRPFHF LFYTGKQSYY S LMHDTYGK LLDVEKHHNQ LRAKDLLAEK TKILKDPIGS RWLIKEELEE MLVEKLSDQD YAQFIRLLER LSALPCGATE ED FVNRFRR SIPIQSKKQL IEPLQYDEQG MAFSRGEGKR KTAKAEVVVY GQGSGRIDVN GVDYLLYFPV TQDREQLMFP LHF LDRLGK HDMTCAVSGG GRSAQAGAVR LAMARALCSF VTEDEVEWMR QAGLLTADPR VRERKKPGQE GARRKFTWKK R

UniProtKB: 28S ribosomal protein S9, mitochondrial

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Macromolecule #35: Mitochondrial ribosomal protein S11

MacromoleculeName: Mitochondrial ribosomal protein S11 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 20.856934 KDa
SequenceString: MQVVRNAGSR LLQSWAWPPK TRIVAGLPAS TIHRSAPHLQ DAAAKLDIED KEEARTPSHS SFSIYPPIPG QENSLRWAGK KFEEIPIAH IKASYNNTQI QVVSAAHQPL AHASCGTEGF RNAKKGTGIA AQTAGIAAAA KATGKGVTHV RVVVKGLGPG R LSAIKGLT ...String:
MQVVRNAGSR LLQSWAWPPK TRIVAGLPAS TIHRSAPHLQ DAAAKLDIED KEEARTPSHS SFSIYPPIPG QENSLRWAGK KFEEIPIAH IKASYNNTQI QVVSAAHQPL AHASCGTEGF RNAKKGTGIA AQTAGIAAAA KATGKGVTHV RVVVKGLGPG R LSAIKGLT MGGLEVISIT DNTPIPH(5F0)GC RPRKARRL

UniProtKB: Mitochondrial ribosomal protein S11

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Macromolecule #36: Mitochondrial ribosomal protein S34

MacromoleculeName: Mitochondrial ribosomal protein S34 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 55.714398 KDa
SequenceString: MRERRLLGRV TQDTHTGRAR IWLHSAPPEA PGRALGLHTV LTAQGVQGLH QDVGSSIFQD GWDGERRVSC HSFFSPRRDT TGVRRADPQ PRQHVDKTLI GSGSQSAGPS VSSSSPTGKP APVPGRLTAF REALPCRSQA DYHLSGLRGT SEESIPPPAA H WARPHLGP ...String:
MRERRLLGRV TQDTHTGRAR IWLHSAPPEA PGRALGLHTV LTAQGVQGLH QDVGSSIFQD GWDGERRVSC HSFFSPRRDT TGVRRADPQ PRQHVDKTLI GSGSQSAGPS VSSSSPTGKP APVPGRLTAF REALPCRSQA DYHLSGLRGT SEESIPPPAA H WARPHLGP RAPPGAARPA RVAAAAVPWP PPCAPPPGPG RSRRPRAGGT LGVRVRDLPG RGPPRSPAPA PARAPGPPSS SV GHGPPGL FCASGRARTP LRETPAASGI GRARRRAAAS SGQSTEAPAA MARKKVRPRL IAELARRVRA LREQRERPRD SQR YALDYE TMMRPHSGNR LPMRAWADVR HESRLLQLLN RLPLFGLGRL VTRKSWLWQH DEPCYWRLTR VRPDYAAENL DHGK AWGIL TFKGKTESEA REIEQVMYHD WRLVPKHEEE AFTAFTLVPE DTPLSVPYPP LLRAMILAQR QKYGDTSTEE PMLNL ERAR IDPWDYPAKL EAKTKTKGAA V

UniProtKB: Mitochondrial ribosomal protein S34

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Macromolecule #3: 12S rRNA

MacromoleculeName: 12S rRNA / type: rna / ID: 3
Details: Corresponds to NCBI reference sequence NC_012095.1 (positions 70-1031)
Number of copies: 1
Source (natural)Organism: Sus scrofa (pig) / Organ: Liver
Molecular weightTheoretical: 309.08725 KDa
SequenceString: ACACAGGUUU GGUCCUGGCC UUUCUAUUAA UUCUUAAUAA AAUUACACAU GCAAGUAUCC GCGCCCCGGU GAGAAUGCCC UCCAGAUCU UAAAGAUCAA AAGGAGCAGG UAUCAAGCAC ACCUAUAACG GUAGCUCAUA ACGCCUUGCU CAACCACACC C CCACGGGA ...String:
ACACAGGUUU GGUCCUGGCC UUUCUAUUAA UUCUUAAUAA AAUUACACAU GCAAGUAUCC GCGCCCCGGU GAGAAUGCCC UCCAGAUCU UAAAGAUCAA AAGGAGCAGG UAUCAAGCAC ACCUAUAACG GUAGCUCAUA ACGCCUUGCU CAACCACACC C CCACGGGA AACAGCAGUG AUAAAAAUUA AGCCAUGAAC GAAAGUUUGA CUAAGUUAUA UUAAUUAGAG UUGGUAAAUC UC GUGCCAG CCACCGCGGU CAUACGAUUA ACCCAAAUUA AUAGAUCCAC GGCGUAAAGA GUGUUUAAGA AAAAAAAUCA CAA UAGAGU UAAAUUAUAA CUAAGCUGUA AAAAGCCCUA GUUAAAAUAA AAUAACCCAC GAAAGUGACU CUAAUAAUCC UGAC ACACG AUAGCUAGGA CCCAAACUGG GA(5MU)UAGAUAC CCCACUAUGC CUAGCCCUAA ACCCAAAUAG UUACAUAACA A AACUAUUC GCCAGAGUAC UACUCGCAAC UGCCUAAAAC UCAAAGGACU UGGCGGUGCU UCACAUCCAC CUAGAGGAGC CU GUUCUAU AAUCGAUAAA CCCCGAUAGA CCUUACCAAC CCUUGCCAAU UCAGCCUAUA UACCGCCAUC UUCAGCAAAC CCU AAAAAG GAACAAUAGU AAGCACAAUC AUAGCACAUA AAAACGUUAG GUCAAGGUGU AGCUUAUGGG UUGGAAAGAA AUGG GCUAC AUUUUCUACA UAAGAAUAUC CACCACACGA AAGUUUUUAU GAAACUAAAA ACCAAAGGAG GAUUUAGCAG UAAAU CAAG AAUAGAGUGC UUGAUUGAAU AAGGCCAUGA AGCACGCACA CACCG(B8T)C(5MC)GU CACCCUCCUC AAGCAUGU A GUAAUAAAAA UAACCUAUAU UCAAUUACAC AACCAUGCAA GAAGAGACAA GUCGUAACAA GGUAAGCAUA CUGG(MA6) (MA6)AGU GUGCUUGGAU UACC

GENBANK: GENBANK: NC_012095.1

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Macromolecule #9: P-site Met-tRNA(fMet)

MacromoleculeName: P-site Met-tRNA(fMet) / type: rna / ID: 9
Details: Corresponds to GenBank sequence ID LC530718.1 (positions 1-71). In vitro transcribed human tRNA with formyl-methionine attached to the CCA-end of the tRNA.
Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.823703 KDa
SequenceString:
AGUAAGGUCA GCUAAAUAAG CUAUCGGGCC CAUACCCCGA AAAUGUUGGU UAUACCCUUC CCGUACUACC A(FME)

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Macromolecule #11: mRNA

MacromoleculeName: mRNA / type: rna / ID: 11 / Details: Synthetic mRNA / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 2.902831 KDa
SequenceString:
AAUGAGACA

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Macromolecule #37: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 37 / Number of copies: 127 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #38: SPERMINE

MacromoleculeName: SPERMINE / type: ligand / ID: 38 / Number of copies: 1 / Formula: SPM
Molecular weightTheoretical: 202.34 Da
Chemical component information

ChemComp-SPM:
SPERMINE

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Macromolecule #39: SPERMIDINE

MacromoleculeName: SPERMIDINE / type: ligand / ID: 39 / Number of copies: 1 / Formula: SPD
Molecular weightTheoretical: 145.246 Da
Chemical component information

ChemComp-SPD:
SPERMIDINE

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Macromolecule #40: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 40 / Number of copies: 11 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #41: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 41 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #42: FE-S-O HYBRID CLUSTER

MacromoleculeName: FE-S-O HYBRID CLUSTER / type: ligand / ID: 42 / Number of copies: 2 / Formula: FS2
Molecular weightTheoretical: 335.508 Da
Chemical component information

ChemComp-FS2:
FE-S-O HYBRID CLUSTER

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Macromolecule #43: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 43 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #44: GUANOSINE-5'-DIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 44 / Number of copies: 1 / Formula: GDP
Molecular weightTheoretical: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

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Macromolecule #45: water

MacromoleculeName: water / type: ligand / ID: 45 / Number of copies: 3 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Component:
ConcentrationFormulaName
20.0 mMC8H18N2O4SHEPES
100.0 mMKClpotassium chloride
20.0 mMMgCl2magnesium chloride

Details: 20mM HEPES-KOH pH=7.5, 100mM KCl, 20mM MgCl2, ~1% sucrose (from mtRF1 storage buffer)
GridModel: Quantifoil R2/2 / Material: COPPER / Support film - #0 - Film type ID: 1 / Support film - #0 - Material: CARBON / Support film - #0 - topology: HOLEY / Support film - #1 - Film type ID: 2 / Support film - #1 - Material: CARBON / Support film - #1 - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
DetailsIn vitro reconstituted complex (55S). Focused refinement on small subunit.

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Specialist opticsEnergy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 6973 / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 81000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

DetailsMicrographs were motion corrected and CTF parameters were estimated using Cryosparc live v4.
Particle selectionNumber selected: 1471587
Details: Particles were picked with blob picker in Cryosparc live v4.
Startup modelType of model: EMDB MAP
EMDB ID:

2914


Details: low-pass filtered to 40A
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4) / Number images used: 50622
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4)
Details: Homogeneous refinement (Cryosparc v4) of all particles with EMD-2914 (low-pass filtered to 40A) as initial reference
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial model
PDB IDChainDetails

7nqh

source_name: PDB, initial_model_type: experimental modelSus scrofa mitoribosome

7qi4

source_name: PDB, initial_model_type: experimental modelHigh-res mammalian mitoribosome

0-f1

source_name: AlphaFold, initial_model_type: in silico modelmtRF1 model prediction
RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-8oip:
28S mammalian mitochondrial small ribosomal subunit with mtRF1 and P-site tRNA

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