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- EMDB-14191: Ternary complex of ribosome nascent chain with SRP and NAC -

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Basic information

Entry
Database: EMDB / ID: EMD-14191
TitleTernary complex of ribosome nascent chain with SRP and NAC
Map dataTernary ribosome-nascent chain, SRP and NAC complex
Sample
  • Complex: Ternary ribosome-nascent chain SRP NAC complex
    • Complex: Ribosome
      • Protein or peptide: x 43 types
      • RNA: x 3 types
    • Complex: SRP RNA 7SL
      • RNA: x 1 types
    • Complex: Signal recognition particle proteins
      • Protein or peptide: x 3 types
    • Complex: Nascent chain preprolactin
      • Protein or peptide: x 1 types
    • Complex: Isoform 2 of Transcription factor BTF3
      • Protein or peptide: x 1 types
  • Ligand: x 2 types
KeywordsRibosome / SRP / NAC / nascent chain / co-translational / Endoplasmic reticulum / co-translational protein targeting / co-translational folding
Function / homology
Function and homology information


PI3K Cascade / PIP3 activates AKT signaling / FLT3 Signaling / Negative regulation of FLT3 / FLT3 signaling through SRC family kinases / RAF/MAP kinase cascade / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / regulation of skeletal muscle fiber development ...PI3K Cascade / PIP3 activates AKT signaling / FLT3 Signaling / Negative regulation of FLT3 / FLT3 signaling through SRC family kinases / RAF/MAP kinase cascade / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / negative regulation of striated muscle cell apoptotic process / regulation of skeletal muscle fiber development / SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition / positive regulation of cell proliferation involved in heart morphogenesis / endoplasmic reticulum signal peptide binding / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / signal recognition particle, endoplasmic reticulum targeting / Major pathway of rRNA processing in the nucleolus and cytosol / GTP hydrolysis and joining of the 60S ribosomal subunit / positive regulation of skeletal muscle tissue growth / cardiac ventricle development / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / granulocyte differentiation / signal recognition particle binding / signal recognition particle / cotranslational protein targeting to membrane / protein targeting to ER / signal-recognition-particle GTPase / dendritic cell differentiation / SRP-dependent cotranslational protein targeting to membrane, translocation / heart trabecula morphogenesis / 7S RNA binding / skeletal muscle tissue regeneration / SRP-dependent cotranslational protein targeting to membrane / exocrine pancreas development / ribosomal subunit / embryonic hemopoiesis / exit from mitosis / optic nerve development / retinal ganglion cell axon guidance / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / SRP-dependent cotranslational protein targeting to membrane / ribonucleoprotein complex binding / protein-RNA complex assembly / rough endoplasmic reticulum / MDM2/MDM4 family protein binding / neutrophil chemotaxis / B cell differentiation / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / cytokine activity / wound healing / receptor tyrosine kinase binding / antimicrobial humoral immune response mediated by antimicrobial peptide / GDP binding / protein transport / regulation of translation / large ribosomal subunit / retina development in camera-type eye / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / defense response to Gram-negative bacterium / killing of cells of another organism / cytosolic large ribosomal subunit / in utero embryonic development / cytoplasmic translation / transcription coactivator activity / tRNA binding / postsynaptic density / rRNA binding / nuclear speck / nuclear body / ribosome / structural constituent of ribosome / protein domain specific binding / translation / response to xenobiotic stimulus / ribonucleoprotein complex / focal adhesion / GTPase activity / mRNA binding / positive regulation of cell population proliferation / synapse / GTP binding / nucleolus / negative regulation of transcription by RNA polymerase II / cell surface / endoplasmic reticulum / positive regulation of transcription by RNA polymerase II / ATP hydrolysis activity / DNA binding / extracellular space / RNA binding / extracellular exosome / zinc ion binding / nucleus
Similarity search - Function
Flt3 ligand / flt3 ligand / Transcription factor BTF3 / Nascent polypeptide-associated complex NAC domain / Nascent polypeptide-associated complex subunit alpha / NAC A/B domain superfamily / NAC domain / NAC A/B domain profile. / NAC / Nascent polypeptide-associated complex subunit alpha-like, UBA domain ...Flt3 ligand / flt3 ligand / Transcription factor BTF3 / Nascent polypeptide-associated complex NAC domain / Nascent polypeptide-associated complex subunit alpha / NAC A/B domain superfamily / NAC domain / NAC A/B domain profile. / NAC / Nascent polypeptide-associated complex subunit alpha-like, UBA domain / HYPK UBA domain / Signal recognition particle subunit SRP68 / Signal recognition particle subunit SRP68, RNA-binding domain / SRP68, N-terminal domain superfamily / RNA-binding signal recognition particle 68 / Signal recognition particle, SRP54 subunit, eukaryotic / Signal recognition particle, SRP19 subunit / Signal recognition particle, subunit SRP19-like superfamily / SRP19 protein / SRP/SRP receptor, N-terminal / Signal recognition particle, SRP54 subunit / Signal recognition particle, SRP54 subunit, M-domain / Signal recognition particle, SRP54 subunit, M-domain superfamily / Signal peptide binding domain / SRP54-type proteins GTP-binding domain signature. / Signal recognition particle SRP54, helical bundle / Signal recognition particle SRP54, N-terminal domain superfamily / SRP54-type protein, helical bundle domain / SRP54-type protein, helical bundle domain / Signal recognition particle, SRP54 subunit, GTPase domain / SRP54-type protein, GTPase domain / SRP54-type protein, GTPase domain / Four-helical cytokine-like, core / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L30e / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / metallochaperone-like domain / TRASH domain / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L10e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L44e signature. / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / : / Ribosomal protein L19, eukaryotic / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L6e signature. / : / Ribosomal protein L44e / Ribosomal protein L44 / : / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L30e signature 1. / Ribosomal L40e family / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal Protein L6, KOW domain / Ribosomal protein L30e signature 2. / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L36e signature. / Ribosomal protein L35Ae, conserved site / Ribosomal protein L30e, conserved site / Ribosomal protein L35Ae signature. / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / : / Ribosomal protein L6e / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L19 / Ribosomal protein L30/YlxQ / Ribosomal protein L7A/L8 / 60S ribosomal protein L6E / 60S ribosomal protein L35
Similarity search - Domain/homology
Small ribosomal subunit protein eS32 / Fms related receptor tyrosine kinase 3 ligand / Ubiquitin-ribosomal protein eL40 fusion protein / Ribosomal protein L36a / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein eL31 ...Small ribosomal subunit protein eS32 / Fms related receptor tyrosine kinase 3 ligand / Ubiquitin-ribosomal protein eL40 fusion protein / Ribosomal protein L36a / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL29 / Uncharacterized protein / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein eL36 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL34 / Signal recognition particle 19 kDa protein / Transcription factor BTF3 / Signal recognition particle subunit SRP54 / Nascent polypeptide-associated complex subunit alpha / Large ribosomal subunit protein eL19 / Signal recognition particle subunit SRP68 / Large ribosomal subunit protein eL14 / Large ribosomal subunit protein eL37
Similarity search - Component
Biological speciesOryctolagus cuniculus (rabbit) / Homo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.83 Å
AuthorsJomaa A / Gamerdinger M / Hsieh H / Wallisch A / Chandrasekaran V / Ulusoy Z / Scaiola A / Hegde R / Shan S / Ban N / Deuerling E
Funding support Switzerland, 1 items
OrganizationGrant numberCountry
Swiss National Science Foundation Switzerland
CitationJournal: Science / Year: 2022
Title: Mechanism of signal sequence handover from NAC to SRP on ribosomes during ER-protein targeting.
Authors: Ahmad Jomaa / Martin Gamerdinger / Hao-Hsuan Hsieh / Annalena Wallisch / Viswanathan Chandrasekaran / Zeynel Ulusoy / Alain Scaiola / Ramanujan S Hegde / Shu-Ou Shan / Nenad Ban / Elke Deuerling /
Abstract: The nascent polypeptide-associated complex (NAC) interacts with newly synthesized proteins at the ribosomal tunnel exit and competes with the signal recognition particle (SRP) to prevent mistargeting ...The nascent polypeptide-associated complex (NAC) interacts with newly synthesized proteins at the ribosomal tunnel exit and competes with the signal recognition particle (SRP) to prevent mistargeting of cytosolic and mitochondrial polypeptides to the endoplasmic reticulum (ER). How NAC antagonizes SRP and how this is overcome by ER targeting signals are unknown. Here, we found that NAC uses two domains with opposing effects to control SRP access. The core globular domain prevented SRP from binding to signal-less ribosomes, whereas a flexibly attached domain transiently captured SRP to permit scanning of nascent chains. The emergence of an ER-targeting signal destabilized NAC's globular domain and facilitated SRP access to the nascent chain. These findings elucidate how NAC hands over the signal sequence to SRP and imparts specificity of protein localization.
History
DepositionJan 25, 2022-
Header (metadata) releaseMar 16, 2022-
Map releaseMar 16, 2022-
UpdateJul 17, 2024-
Current statusJul 17, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.035
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.035
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7qwq
  • Surface level: 0.027
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_14191.png

Downloads & links

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Map

FileDownload / File: emd_14191.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationTernary ribosome-nascent chain, SRP and NAC complex
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 448 pix.
= 483.84 Å		1.08 Å/pix.
x 448 pix.
= 483.84 Å		1.08 Å/pix.
x 448 pix.
= 483.84 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.08 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.027 / Movie #1: 0.035
Minimum - Maximum-0.09441678 - 0.2096099
Average (Standard dev.)0.00049410004 (±0.0102524785)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions448448448
Spacing448448448
CellA=B=C: 483.84003 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.081.081.08
M x/y/z448448448
origin x/y/z0.0000.0000.000
length x/y/z483.840483.840483.840
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS448448448
D min/max/mean-0.0940.2100.000

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Supplemental data

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Sample components

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Entire : Ternary ribosome-nascent chain SRP NAC complex

EntireName: Ternary ribosome-nascent chain SRP NAC complex
Components
  • Complex: Ternary ribosome-nascent chain SRP NAC complex
    • Complex: Ribosome
      • Protein or peptide: L8
      • Protein or peptide: 60S ribosomal protein L29
      • RNA: 28S rRNA
      • Protein or peptide: uL3
      • Protein or peptide: 60S ribosomal protein L30
      • Protein or peptide: 60S ribosomal protein L4
      • Protein or peptide: Ribosomal protein L31
      • Protein or peptide: Ribosomal_L18_c domain-containing protein
      • Protein or peptide: Ribosomal protein L32
      • Protein or peptide: 60S ribosomal protein L6
      • Protein or peptide: 60S ribosomal protein L35a
      • Protein or peptide: uL30
      • Protein or peptide: 60S ribosomal protein L34
      • Protein or peptide: 60S ribosomal protein L7a
      • Protein or peptide: 60S ribosomal protein L35
      • Protein or peptide: 60S ribosomal protein L9
      • Protein or peptide: 60S ribosomal protein L36
      • Protein or peptide: 60S ribosomal protein L10
      • Protein or peptide: Ribosomal protein L37
      • Protein or peptide: Ribosomal protein L11
      • Protein or peptide: 60S ribosomal protein L38
      • Protein or peptide: 60S ribosomal protein L13
      • Protein or peptide: Ribosomal protein L39
      • Protein or peptide: 60S ribosomal protein L14
      • Protein or peptide: Ubiquitin A-52 residue ribosomal protein fusion product 1
      • Protein or peptide: Ribosomal protein L15
      • Protein or peptide: 60s ribosomal protein l41
      • Protein or peptide: 60S ribosomal protein L13a
      • Protein or peptide: eL42
      • Protein or peptide: 60S ribosomal protein L17
      • Protein or peptide: eL43
      • Protein or peptide: eL18
      • Protein or peptide: 60S ribosomal protein L28
      • Protein or peptide: 60S ribosomal protein L19
      • Protein or peptide: 60S ribosomal protein L18a
      • Protein or peptide: 60S ribosomal protein L21
      • Protein or peptide: Ribosomal protein L22
      • Protein or peptide: Ribosomal protein L23
      • Protein or peptide: Ribosomal protein L24
      • Protein or peptide: Ribosomal_L23eN domain-containing protein
      • RNA: 5S ribosomal RNA
      • Protein or peptide: Ribosomal protein L26
      • RNA: 5.8S ribosomal RNA
      • Protein or peptide: 60S ribosomal protein L27
      • Protein or peptide: 60S ribosomal protein L27a
      • Protein or peptide: Nascent polypeptide-associated complex subunit alpha
    • Complex: SRP RNA 7SL
      • RNA: SRP RNA 7SL
    • Complex: Signal recognition particle proteins
      • Protein or peptide: Signal recognition particle 19 kDa protein
      • Protein or peptide: Signal recognition particle subunit SRP68
      • Protein or peptide: Signal recognition particle 54 kDa protein
    • Complex: Nascent chain preprolactin
      • Protein or peptide: Nascent chain preprolactin
    • Complex: Isoform 2 of Transcription factor BTF3
      • Protein or peptide: Isoform 2 of Transcription factor BTF3
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: Ternary ribosome-nascent chain SRP NAC complex

SupramoleculeName: Ternary ribosome-nascent chain SRP NAC complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#52

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Supramolecule #2: Ribosome

SupramoleculeName: Ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #7-#52
Source (natural)Organism: Oryctolagus cuniculus (rabbit)

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Supramolecule #3: SRP RNA 7SL

SupramoleculeName: SRP RNA 7SL / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #4: Signal recognition particle proteins

SupramoleculeName: Signal recognition particle proteins / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #2, #5-#6
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #5: Nascent chain preprolactin

SupramoleculeName: Nascent chain preprolactin / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #3
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #6: Isoform 2 of Transcription factor BTF3

SupramoleculeName: Isoform 2 of Transcription factor BTF3 / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #4
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: SRP RNA 7SL

MacromoleculeName: SRP RNA 7SL / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 54.095184 KDa
SequenceString:
CGCUAUGCCG AUCGGGUGUC CGCACUAAGU UCGGCAUCAA UAUGGUGACC UCCCGGGAGC GGGGGACCAC CAGGUUGCCU AAGGAGGGG UGAACCGGCC CAGGUCGGAA ACGGAGCAGG UCAAAACUCC CGUGCUGAUC AGUAGUGGGA UCGCGCCUGU G AAUAGCC

GENBANK: GENBANK: X04249.1

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Macromolecule #9: 28S rRNA

MacromoleculeName: 28S rRNA / type: rna / ID: 9 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 1.539032 MDa
SequenceString: CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC ...String:
CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC GCUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA GAGA GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUCAACCCG GCGGC GGGU CCGGCCGUGU CGGCGGUCUC GGCGGAUCUU UCCCGCUCCC CGUUCCUCCC GACCCCUCCA CCCGCUCCCC CUCCCC CGC CGGUCUUCUC CCUUCACGGG GGGUGGGUCG GCGGGUGCGG GGGUGGGCGG GCGGGGCCGG GGGUGGGGUC GGCGGGG GA CCGUCCCCCG ACCGGCGACC GGCCGCCGCC GGGCGCAUUU CCACCGCGGC GGUGCGCCGC GACCGGCUCC GGGACGGC U GGGAAGGCCC GGCGGGGAAG GUGGCUCGGG GGGACGCCUC GCCGCCGUCG UCCGCGGCGG CGGCGGGCGC GACCCCUCC CCGAGUGUUA CAGCCCCCCC GGCAGCAGCA CUCGCCGAAU CCCGGGGCCG AGGGAGCGAG ACCCGUCGCC GCGCUCUCCC CCCUCCCGG CGCUCCCCCC CGCGGGGGUC CCCCGCGAGG GGGCUCCCCC CGCGGGGGCG CGCCGGUGUC GCCGCCCUCG C GGGCGCGG CACGGGGGGG CCGGGCCACC CCUCCCACGG CGCGACCGCU CUCCCGGCCC CCGCCGCCGC CUCUUCGGGC GU GCGGCGG GGGCGGGGCG GACUGUCCCC AGUGCGCCCC GGGCGGGUCG CGCCGUCGGG CCCGGGGGAG AAGUCGGCCA CGG GGCCAC GCGCGCCUCC GCGUCCCCCC CUCGCCGUCU CCGGGCGGCG GGGGGGGGUU UCGGCGCGGU GUUCUCGGCG CGCG CGCGC GAGCGAGCGC ACGGGGUCGG CGGCGACGUC GGCUACCCAC CCGACCCGUC UUGAAACACG GACCAAGGAG UCUAA CACG UGCGCGAGUC GGGGGCUCGC ACGAAAGCCG CCGUGGCGCA AUGAAGGUGA AGGCCGGCGC GCUCGCCGGC CGAGGU GGG AUCCCGAGGC CUCUCCAGUC CGCCGAGGGC GCACCACCGG CCCGUCUCGC CCGCCGCGCC GGGGAGGUGG AGCACGA GC GCACGUGUUA GGACCCGAAA GAUGGUGAAC UAUGCCUGGG CAGGGCGAAG CCAGAGGAAA CUCUGGUGGA GGUCCGUA G CGGUCCUGAC GUGCAAAUCG GUCGUCCGAC CUGGGUAUAG GGGCGAAAGA CUAAUCGAAC CAUCUAGUAG CUGGUUCCC UCCGAAGUUU CCCUCAGGAU AGCUGGCGCU CUCGCAACCA CGCAGUUUUA UCCGGUAAAG CGAAUGAUUA GAGGUCUUGG GGCCGAAAC GAUCUCAACC UAUUCUCAAA CUUUAAAUGG GUAAGAAGCC CGGCUCGCUG GCGUGAGCCG GGCGUGGAAU G CGAGUGCC UAGUGGGCCA CUUUUGGUAA GCAGAACUGG CGCUGCGGGA UGAACCGAAC GCCGGGUUAA GGCGCCCGAU GC CGACGCU CAUCAGACCC CAGAAAAGGU GUUGGUUGAU AUAGACAGCA GGACGGUGGC CAUGGAAGUC GGAAUCCGCU AAG GAGUGU GUAACAACUC ACCUGCCGAA UCAACUAGCC CUGAAAAUGG AUGGCGCUGG AGCGUCGGGC CCAUACCCGG CCGU CGCCG GCAGUCGAGA GUGGACGGGA GCGCGGGACU CCCCCCGCGG GGGUCCCCCG CCCCCGGAGC CCCGCGGACG CUACG CCGC GACGAGUAGG AGGGCCGCUG CGGUGAGCCU UGAAGCCUAG GGCGCGGGCC CGGGUGGAGC CGCCGCAGGU GCAGAU CUU GGUGGUAGUA GCAAAUAUUC AAACGAGAAC UUUGAAGGCC GAAGUGGAGA AGGGUUCCAU GUGAACAGCA GUUGAAC AU GGGUCAGUCG GUCCUGAGAG AUGGGCGAGC GCCGUUCCGA AGGGACGGGC GAUGGCCUCC GUUGCCCUCG GCCGAUCG A AAGGGAGUCG GGUUCAGAUC CCCGAAUCCG GAGUGGCGGA GAUGGGCGCC GCGAGGCGUC CAGUGCGGUA ACGCGACCG AUCCCGGAGA AGCCGGCGGG AGCCCCGGGG AGAGUUCUCU UUUCUUUGUG AAGGGCAGGG CGCCCUGGAA UGGGUUCGCC CCGAGAGAG GGGCCCGUGC CUUGGAAAGC GUCGCGGUUC CGGCGGCGUC CGGUGAGCUC UCGCUGGCCC UUGAAAAUCC G GGGGAGAG GGUGUAAAUC UCGCGCCGGG CCGUACCCAU AUCCGCAGCA GGUCUCCAAG GUGAACAGCC UCUGGCAUGU UG GAACAAU GUAGGUAAGG GAAGUCGGCA AGCCGGAUCC GUAACUUCGG GAUAAGGAUU GGCUCUAAGG GCUGGGUCGG UCG GGCUGG GGCGCGAAGC GGGGCUGGGC GCGCGCCGCG GCUGGACGAG GCGCCGCCGC CCCCCCCACG CCCGGGGCGC CCUC CCCCG GCCGGGUCCG CUCCCUCGCG CGCGCGUGCG CCGUGGCGCG CGCCGCGCGC GCGGCCGAGC CCCGUCUCCC UCCCC CCCU CUCCCCUCCC GGGGCGGGGU GCGGGGAGGG GCGGGCGGGG CCGUCGCUCG CCGCGCGUGC GCGUCGCUGC GCGUGC GCC GCGCGGGGGG UCCGGCGGGC CUCCGGACGG GGGCUCCGGG CACCCGGGCA CCCGGGGGGC CGGCGGCGGC GGCGACU CU GGACGCGAGC CGGGCCCUUC CCGUGGAUCG CCCCAGCUGC GGCGGGCGUC GCGGCCGCCC UCGGGGAGCC CGGCGGGC G CCGCGUGCGC GCGCGUGCGC GCCCGGGGUC GGGGGGGUGC GAGGCAGGGG AGGCCCUCUC UCCUCCUCCC CCACCCCGG CGCCGCGGCG CCGCGCGUGC GCGCGCGGUC UCCCCCCGCC GGGUCCGCCC CCGGGGCCGC GGUUCCGCGC GGCGCCUCGC CUCGGCCGG CGCCUAGCAG CCGACUUAGA ACUGGUGCGG ACCAGGGGAA UCCGACUGUU UAAUUAAAAC AAAGCAUCGC G AAGGCCCG CGGCGGGUGU UGACGCGAUG UGAUUUCUGC CCAGUGCUCU GAAUGUCAAA GUGAAGAAAU UCAAUGAAGC GC GGGUAAA CGGCGGGAGU AACUAUGACU CUCUUAAGGU AGCCAAAUGC CUCGUCAUCU AAUUAGUGAC GCGCAUGAAU GGA UGAACG AGAUUCCCAC UGUCCCUACC UACUAUCCAG CGAAACCACA GCCAAGGGAA CGGGCUUGGC GGAAUCAGCG GGGA AAGAA GACCCUGUUG AGCUUGACUC UAGUCUGGCA CGGUGAAGAG ACAUGAGAGG UGUAGAAUAA GUGGGAGGCC CCCGG CGCC CUCCCGCCCC CGCGAGGGGG CGGGGCGGGG UCCGCCGGCC UUGCGGGCCG CCGGUGAAAU ACCACUACUC UUAUCG UUU UUUCACUGAC CCGGUGAGGC GGGGGGGCGA GCCCCGAGGG GCUCUCGCUU CUGGCGCCAA GCGCCCGGCC GCGCGCC GG CCGGGCGCGA CCCGCUCCGG GGACAGUGCC AGGUGGGGAG UUUGACUGGG GCGGUACACC UGUCAAACGG UAACGCAG G UGUCCUAAGG CGAGCUCAGG GAGGACAGAA ACCUCCCGUG GAGCAGAAGG GCAAAAGCUC GCUUGAUCUU GAUUUUCAG UACGAAUACA GACCGUGAAA GCGGGGCCUC ACGAUCCUUC UGACCUUUUG GGUUUUAAGC AGGAGGUGUC AGAAAAGUUA CCACAGGGA UAACUGGCUU GUGGCGGCCA AGCGUUCAUA GCGACGUCGC UUUUUGAUCC UUCGAUGUCG GCUCUUCCUA U CAUUGUGA AGCAGAAUUC ACCAAGCGUU GGAUUGUUCA CCCACUAAUA GGGAACGUGA GCUGGGUUUA GACCGUCGUG AG ACAGGUU AGUUUUACCC UACUGAUGAU GUGUUGUUGC CAUGGUAAUC CUGCUCAGUA CGAGAGGAAC CGCAGGUUCA GAC AUUUGG UGUAUGUGCU UGGCUGAGGA GCCAAUGGGG CGAAGCUACC AUCUGUGGGA UUAUGACUGA ACGCCUCUAA GUCA GAAUC CCGCCCAGGC GGAACGAUAC GGCAGCGCCG CGGAGCCUCG GUUGGCCUCG GAUAGCCGGU CCCCCGCCGU CCCCG CCGG CGGCCCGCGU CGUGCGUCCG CCUCGGCGGC GUGCGGCGCG CCCCCCGCCG CGCGUCGGGA CCGGGGUCCG GUGCGG AGU GCCCUUCGUC CUGGGAAACG GGGCGCGGCC GGAGAGGCGG CCGCCCCCUC GCCCGUCACG CACGCCGUUC GUGGGGA AC CUGGCGCUAA ACCAUUCGUA GACGACCUGC UUCUGGGUCG GGGUUUCGUA CGUAGCAGAG CAGCUCCCUC GCUGCGAU C UAUUGAAAGU CAGCCCUCGA CACAAGGGUU UGU

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Macromolecule #47: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 47 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 38.691914 KDa
SequenceString:
GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU U

GENBANK: GENBANK: 4CXE_4

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Macromolecule #49: 5.8S ribosomal RNA

MacromoleculeName: 5.8S ribosomal RNA / type: rna / ID: 49 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 50.143648 KDa
SequenceString:
CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCU

GENBANK: GENBANK: 4CXE_3

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Macromolecule #2: Signal recognition particle 19 kDa protein

MacromoleculeName: Signal recognition particle 19 kDa protein / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 16.183746 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MACAAARSPA DQDRFICIYP AYLNNKKTIA EGRRIPISKA VENPTATEIQ DVCSAVGLNV FLEKNKMYSR EWNRDVQYRG RVRVQLKQE DGSLCLVQFP SRKSVMLYAA EMIPKLKTRT QKTGGADQSL QQGEGSKKGK GKKKK

UniProtKB: Signal recognition particle 19 kDa protein

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Macromolecule #3: Nascent chain preprolactin

MacromoleculeName: Nascent chain preprolactin / type: protein_or_peptide / ID: 3 / Details: this will need to changed to UNK / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 9.549763 KDa
Recombinant expressionOrganism: Oryctolagus cuniculus (rabbit)
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #4: Isoform 2 of Transcription factor BTF3

MacromoleculeName: Isoform 2 of Transcription factor BTF3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 17.724037 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MKETIMNQEK LAKLQAQVRI GGKGTARRKK KVVHRTATAD DKKLQFSLKK LGVNNISGIE EVNMFTNQGT VIHFNNPKVQ ASLAANTFT ITGHAETKQL TEMLPSILNQ LGADSLTSLR RLAEALPKQS VDGKAPLATG EDDDDEVPDL VENFDEASKN E AN

UniProtKB: Transcription factor BTF3

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Macromolecule #5: Signal recognition particle subunit SRP68

MacromoleculeName: Signal recognition particle subunit SRP68 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 70.831578 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MAAEKQVPGG GGGGGSGGGG GSGGGGSGGG RGAGGEENKE NERPSAGSKA NKEFGDSLSL EILQIIKESQ QQHGLRHGDF QRYRGYCSR RQRRLRKTLN FKMGNRHKFT GKKVTEELLT DNRYLLLVLM DAERAWSYAM QLKQEANTEP RKRFHLLSRL R KAVKHAEE ...String:
MAAEKQVPGG GGGGGSGGGG GSGGGGSGGG RGAGGEENKE NERPSAGSKA NKEFGDSLSL EILQIIKESQ QQHGLRHGDF QRYRGYCSR RQRRLRKTLN FKMGNRHKFT GKKVTEELLT DNRYLLLVLM DAERAWSYAM QLKQEANTEP RKRFHLLSRL R KAVKHAEE LERLCESNRV DAKTKLEAQA YTAYLSGMLR FEHQEWKAAI EAFNKCKTIY EKLASAFTEE QAVLYNQRVE EI SPNIRYC AYNIGDQSAI NELMQMRLRS GGTEGLLAEK LEALITQTRA KQAATMSEVE WRGRTVPVKI DKVRIFLLGL ADN EAAIVQ AESEETKERL FESMLSECRD AIQVVREELK PDQKQRDYIL EGEPGKVSNL QYLHSYLTYI KLSTAIKRNE NMAK GLQRA LLQQQPEDDS KRSPRPQDLI RLYDIILQNL VELLQLPGLE EDKAFQKEIG LKTLVFKAYR CFFIAQSYVL VKKWS EALV LYDRVLKYAN EVNSDAGAFK NSLKDLPDVQ ELITQVRSEK CSLQAAAILD ANDAHQTETS SSQVKDNKPL VERFET FCL DPSLVTKQAN LVHFPPGFQP IPCKPLFFDL ALNHVAFPPL EDKLEQKTKS GLTGYIKGIF GFRS

UniProtKB: Signal recognition particle subunit SRP68

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Macromolecule #6: Signal recognition particle 54 kDa protein

MacromoleculeName: Signal recognition particle 54 kDa protein / type: protein_or_peptide / ID: 6 / Details: mutant G226E / Number of copies: 1 / Enantiomer: LEVO
EC number: Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 55.847734 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK QLRENVKSAI DLEEMASGLN KRKMIQHAVF KELVKLVDP GVKAWTPTKG KQNVIMFVGL QGSGKTTTCS KLAYYYQRKG WKTCLICADT FRAGAFDQLK QNATKARIPF Y GSYTEMDP ...String:
MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK QLRENVKSAI DLEEMASGLN KRKMIQHAVF KELVKLVDP GVKAWTPTKG KQNVIMFVGL QGSGKTTTCS KLAYYYQRKG WKTCLICADT FRAGAFDQLK QNATKARIPF Y GSYTEMDP VIIASEGVEK FKNENFEIII VDTSGRHKQE DSLFEEMLQV ANAIQPDNIV YVMDASIEQA CEAQAKAFKD KV DVASVIV TKLDGHAKGG GALSAVAATK SPIIFIGTGE HIDDFEPFKT QPFISKLLGM GDIEGLIDKV NELKLDDNEA LIE KLKHGQ FTLRDMYEQF QNIMKMGPFS QILGMIPGFG TDFMSKGNEQ ESMARLKKLM TIMDSMNDQE LDSTDGAKVF SKQP GRIQR VARGSGVSTR DVQELLTQYT KFAQMVKKMG GIKGLFKGGD MSKNVSQSQM AKLNQQMAKM MDPRVLHHMG GMAGL QSMM RQFQQGAAGN MKGMMGFNNM

UniProtKB: Signal recognition particle subunit SRP54

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Macromolecule #7: L8

MacromoleculeName: L8 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 26.570105 KDa
SequenceString: GRVIRGQRKG AGSVFRAHVK HRKGAARLRA VDFAERHGYI KGIVKDIIHD PGRGAPLAKV VFRDPYRFKK RTELFIAAEG IHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS A NRAVVGVV ...String:
GRVIRGQRKG AGSVFRAHVK HRKGAARLRA VDFAERHGYI KGIVKDIIHD PGRGAPLAKV VFRDPYRFKK RTELFIAAEG IHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS A NRAVVGVV AGGGRIDKPI LKAGRAYHKY KAKRNCWPRV RGVAMNPVEH PFGGGNHQHI GKPSTIRRDA PAGRKVGLIA AR RTGR

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #8: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 24.608236 KDa
SequenceString: MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALVKPKE VKPTIPKGV SRKLDRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGKAQA E TKPKAQAQ ...String:
MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALVKPKE VKPTIPKGV SRKLDRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGKAQA E TKPKAQAQ AKPKAQAQGK PKAQAQAKPK AHAQAKPKAQ AQAKPKAQAQ TKRTGVTPGL LSAWG

UniProtKB: Large ribosomal subunit protein eL29

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Macromolecule #10: uL3

MacromoleculeName: uL3 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 46.107977 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DAGKRQLDKD FSSMKKYCQV I RVLAHTQM ...String:
MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DAGKRQLDKD FSSMKKYCQV I RVLAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVS QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKTHRG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINPL GGF VHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTVEEKK AFMGPLKKDR IAKE EGA

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #11: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 12.821092 KDa
SequenceString:
MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQTLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLSIIDP GDSDIIRSMP EQTGEK

UniProtKB: Large ribosomal subunit protein eL30

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Macromolecule #12: 60S ribosomal protein L4

MacromoleculeName: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 46.388855 KDa
SequenceString: MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV ...String:
MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC VIYNEDNGIV KAFRNIPGIT LLNVTKLNIL KL APGGHVG RFCIWTESAF RKLDDLYGTW RKAASLKSNY NLPMHKMLNT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVERAAA ALAAKSDPKE APAKKKPVVG KKKKPVVGRK AAAAKKPAAD KKAA DKRAG PEDKKPAA

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #13: Ribosomal protein L31

MacromoleculeName: Ribosomal protein L31 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.494938 KDa
SequenceString:
MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

UniProtKB: Large ribosomal subunit protein eL31

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Macromolecule #14: Ribosomal_L18_c domain-containing protein

MacromoleculeName: Ribosomal_L18_c domain-containing protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 34.481828 KDa
SequenceString: MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD ...String:
MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NNVTPDMMEE MY KKAHAAI RENPVYEKKP KREVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #15: Ribosomal protein L32

MacromoleculeName: Ribosomal protein L32 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 18.350049 KDa
SequenceString:
MCLLLISFLY LKEKKGCPLL GTIAALRPLV KPKIVKKRTK KFIRHQSDRY VKIKRNWRKP RGIDNRVRRR FKGQILMPNI GYGSNKKTK HMLPSGFRKF LVHNVKELEV LLMCNKSYCA EIAHNVSSKN RKAIVERAAQ LAIRVTNPNA RLRSEENE

UniProtKB: Large ribosomal subunit protein eL32

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Macromolecule #16: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 33.055297 KDa
SequenceString: MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGRKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL ...String:
MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGRKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL KQLSSGLLLV TGPLSLNRVP LRRTHQKFVI ATSTKIDISG VKIPKHLTDA YFKKKQLRKP RHQEGEIFDT EK EKYEITE QRKVDQKAVD SQILPKIKAV PQLQGYLRSV FALTNGVYPH KLVF

UniProtKB: Large ribosomal subunit protein eL6

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Macromolecule #17: 60S ribosomal protein L35a

MacromoleculeName: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 12.580809 KDa
SequenceString:
MSGRLWCKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

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Macromolecule #18: uL30

MacromoleculeName: uL30 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 26.662787 KDa
SequenceString: NFAELKIKRL RKKFAQKMLR KARRKLIYEK AKHYHKEYRQ MYRTEIRMAR MARKAGNFYV PAEPKLAFVI RIRGINGVSP KVRKVLQLL RLRQIFNGTF VKLNKASINM LRIVEPYIAW GYPNLKSVNE LIYKRGYGKI NKKRIALTDN ALIARSLGKY G IICMEDLI ...String:
NFAELKIKRL RKKFAQKMLR KARRKLIYEK AKHYHKEYRQ MYRTEIRMAR MARKAGNFYV PAEPKLAFVI RIRGINGVSP KVRKVLQLL RLRQIFNGTF VKLNKASINM LRIVEPYIAW GYPNLKSVNE LIYKRGYGKI NKKRIALTDN ALIARSLGKY G IICMEDLI HEIYTVGKRF KEANNFLWPF KLSSPRGGMK KKTTHFVEGE DAGNREDQIN RLIRRMN

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #19: 60S ribosomal protein L34

MacromoleculeName: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.557543 KDa
SequenceString:
MSLPGRCLEA ATMVQRLTYR RRLSYNTASN KTRLSRTPGN RIVYLYTKKV GKAPKSACGV CPGRLRGVRA VRPKVLMRLS KTKKHVSRA YGGSMCAKCV RDRIKRAFLI EEQKIVVKVL KAQAQSQKAK

UniProtKB: Large ribosomal subunit protein eL34

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Macromolecule #20: 60S ribosomal protein L7a

MacromoleculeName: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 36.267605 KDa
SequenceString: MSSYRLGYCM KEERHNLVLC LWSQSPGILN SKCLWPFTNI HLLVGALPRE GAGGAWGGGR SEQLPTCSTT HHDFTWDKKV VNPLFEKRP KNFGIGQDIQ PKRDLTRFVK WPRYIRLQRQ RAILYKRLKV PPAINQFTQV LDRQTATQLL KLAHKYRPET K QEKKQRLL ...String:
MSSYRLGYCM KEERHNLVLC LWSQSPGILN SKCLWPFTNI HLLVGALPRE GAGGAWGGGR SEQLPTCSTT HHDFTWDKKV VNPLFEKRP KNFGIGQDIQ PKRDLTRFVK WPRYIRLQRQ RAILYKRLKV PPAINQFTQV LDRQTATQLL KLAHKYRPET K QEKKQRLL ARAEKKAAGK GDVPTKRPPV LRAGVNTVTT LVENKKAQLV VIAHDVDPIE LVVFLPALCR KMGVPYCILK GK ARLCRLV HRKTCTTVAF TQVNSEDKGA LAKLVEAIRT NYNDRYDEIR RHWGGNVLGP KSVARIAKLE KAKAKELATK LG

UniProtKB: Large ribosomal subunit protein eL8

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Macromolecule #21: 60S ribosomal protein L35

MacromoleculeName: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.566599 KDa
SequenceString:
MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ESLKTKKQQR KERLYPLRKY AVKA

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #22: 60S ribosomal protein L9

MacromoleculeName: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 21.871418 KDa
SequenceString: MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK ...String:
MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #23: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 11.888371 KDa
SequenceString:
ALRYPMAVGL NKGHKVTKNV SKPRHSRRRG RLTKHTKFVR DMIREVCGFA PYERRAMELL KVSKDKRALK FIKKRVGTHI RAKRKREEL SSVLAAMRKA AAK

UniProtKB: Large ribosomal subunit protein eL36

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Macromolecule #24: 60S ribosomal protein L10

MacromoleculeName: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 24.643057 KDa
SequenceString: MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS ...String:
MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAEK RLIPDGCGVK YIPNRGPLDK WRALHS

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #25: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 11.111032 KDa
SequenceString:
MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Large ribosomal subunit protein eL37

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Macromolecule #26: Ribosomal protein L11

MacromoleculeName: Ribosomal protein L11 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 20.288465 KDa
SequenceString:
MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #27: 60S ribosomal protein L38

MacromoleculeName: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 8.107752 KDa
SequenceString:
PRKIEEIKDF LLTARRKDAK SVKIKKNKDN VKFKVRCSRY LYTLVITDKE KAEKLKQSLP PGLAVKELK

UniProtKB: Large ribosomal subunit protein eL38

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Macromolecule #28: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 28
Details: APSRNGMILKPHFHKDWQRRVATWFNQPARKIRRRKARQARARRIAPRPASGPLRPIVRCPTVRYHTKVRAGRGFSLEELRVAGIHKKVARTIGISVDPR ...Details: APSRNGMILKPHFHKDWQRRVATWFNQPARKIRRRKARQARARRIAPRPASGPLRPIVRCPTVRYHTKVRAGRGFSLEELRVAGIHKKVARTIGISVDPR RRNKSTESLQANVQRLKEYRSKLVLFPRKPSAPKKGDSSAEELKLATQLTGPVMPIRNVFKKEKARVITEEEKNFKAFASLRMARANARLFGIRAKRAKE AAEQDVEKKK
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 24.216525 KDa
SequenceString: APSRNGMILK PHFHKDWQRR VATWFNQPAR KIRRRKARQA RARRIAPRPA SGPLRPIVRC PTVRYHTKVR AGRGFSLEEL RVAGIHKKV ARTIGISVDP RRRNKSTESL QANVQRLKEY RSKLVLFPRK PSAPKKGDSS AEELKLATQL TGPVMPIRNV F KKEKARVI ...String:
APSRNGMILK PHFHKDWQRR VATWFNQPAR KIRRRKARQA RARRIAPRPA SGPLRPIVRC PTVRYHTKVR AGRGFSLEEL RVAGIHKKV ARTIGISVDP RRRNKSTESL QANVQRLKEY RSKLVLFPRK PSAPKKGDSS AEELKLATQL TGPVMPIRNV F KKEKARVI TEEEKNFKAF ASLRMARANA RLFGIRAKRA KEAAEQDVEK KK

UniProtKB: Uncharacterized protein

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Macromolecule #29: Ribosomal protein L39

MacromoleculeName: Ribosomal protein L39 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 6.426759 KDa
SequenceString:
MSSHKTFRIK RFLAKKQKQN RPIPQWIRMK TGNKIRYNSK RRHWRRTKLG L

UniProtKB: Large ribosomal subunit protein eL39

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Macromolecule #30: 60S ribosomal protein L14

MacromoleculeName: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 23.810432 KDa
SequenceString: AVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG ...String:
AVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG KKAAAQKAPA QKAPAQKAAG QKAAQPPKAQ KGQKPPAQKA PAPKASGKKA

UniProtKB: Large ribosomal subunit protein eL14

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Macromolecule #31: Ubiquitin A-52 residue ribosomal protein fusion product 1

MacromoleculeName: Ubiquitin A-52 residue ribosomal protein fusion product 1
type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.758394 KDa
SequenceString:
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGIIEP SLRQLAQKY NCDKMICRKC YARLHPRAVN CRKKKCGHTN NLRPKKKVK

UniProtKB: Ubiquitin-ribosomal protein eL40 fusion protein

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Macromolecule #32: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 24.207285 KDa
SequenceString: MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG ...String:
MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Large ribosomal subunit protein eL15

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Macromolecule #33: 60s ribosomal protein l41

MacromoleculeName: 60s ribosomal protein l41 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 3.473451 KDa
SequenceString:
MRAKWRKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

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Macromolecule #34: 60S ribosomal protein L13a

MacromoleculeName: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 56.501578 KDa
SequenceString: MVVPAPAWSP TTSLLLLLLL LLLSPGLLGS PDCSFPHSPI SSTFANTIRQ LSDYLLQDYP VTVASNLQDD ELCGAFWRLV LAQRWMGQL KTVAGSQMQK LLEAVNTEIV FVTSCALQPL PSCLRFVQAN ISHLLQDTSQ QLVALKPWIT RRNFSRCLEL Q CQPDPSTL ...String:
MVVPAPAWSP TTSLLLLLLL LLLSPGLLGS PDCSFPHSPI SSTFANTIRQ LSDYLLQDYP VTVASNLQDD ELCGAFWRLV LAQRWMGQL KTVAGSQMQK LLEAVNTEIV FVTSCALQPL PSCLRFVQAN ISHLLQDTSQ QLVALKPWIT RRNFSRCLEL Q CQPDPSTL LPPRSPGALE ATSLPAPQAS LLLLLLLLLL PAALLLLATA WCLRRRRRRR RMPCPGERPW TPRSDGNDTD WS ARRRPAL QWGLSAHALD TLANEKTGNG LCCVTSSAPV SRDKKPCAKS SFFPRQLPKM AEGQQVLVLD GRGHLLGRLA AIV AKQVLL GRKVVVVRCE GINISGNFYR NKLKYLAFLR KRMNTNPSRG PYHFRAPSRI FWRTVRGMLP HKTKRGQAAL DRLK VFDGI PPPYDKKKRM VVPAALKVVR LKPTRKFAYL GRLAHEVGWK YQAVTATLEE KRKEKAKIHY RKKKQLMRLR KQAEK NVEK KIDRFTEVLK THGFLV

UniProtKB: Fms related receptor tyrosine kinase 3 ligand

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Macromolecule #35: eL42

MacromoleculeName: eL42 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 16.130169 KDa
SequenceString:
MVAPIASHEE VRLGTAYILP LQAGSLSFRA DSVPAMVNVP KTRRTFCKKC GKHQPHKVTQ YKKGKDSLYA QGKRRYDRKQ SGYGGQTKP IFRKKAKTTK KIVLRLECVE PNCRSKRMLA IKRCKHFELG GDKKRKGQVI QF

UniProtKB: Ribosomal protein L36a

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Macromolecule #36: 60S ribosomal protein L17

MacromoleculeName: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.758678 KDa
SequenceString:
VRYSLDPENP TKSCKSRGSN LRVHFKNTRE TAQAIKGMHI RKATKYLKDV TLKKQCVPFR RYNGGVGRCA QAKQWGWTQG RWPKKSAEF LLHMLKNAES NAELKGLDVD SLVIEHIQVN KAPKMRRRTY RAHGRINPYM SSPCHIEMIL TEKE

+
Macromolecule #37: eL43

MacromoleculeName: eL43 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 10.29935 KDa
SequenceString:
MAKRTKKVGI VGKYGTRYGA SLRKMVKKIE ISQHAKYTCS FCGKTKMKRR AVGIWHCGSC MKTVAGGAWT YNTTSAVTVK SAIRRLKEL KDQ

UniProtKB: Large ribosomal subunit protein eL43

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Macromolecule #38: eL18

MacromoleculeName: eL18 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 21.457391 KDa
SequenceString:
GVDIRHNKDR KVRRKEPKSQ DIYLRLLVKL YRFLARRTNS TFNQVVLKRL FMSRTNRPPL SLSRMIRKMK LPGREGKTAV VVGTITDDV RVQEVPKLKV CALRVSSRAR SRILKAGGKI LTFDQLALDS PKGCGTVLLS GPRKGREVYR HFGKAPGTPH S HTKPYVRS KGRKFERARG RRASRGYKN

+
Macromolecule #39: 60S ribosomal protein L28

MacromoleculeName: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 15.783614 KDa
SequenceString:
MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVMKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYHPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

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Macromolecule #40: 60S ribosomal protein L19

MacromoleculeName: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 23.535281 KDa
SequenceString: MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ...String:
MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

UniProtKB: Large ribosomal subunit protein eL19

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Macromolecule #41: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 20.696363 KDa
SequenceString:
KASGTLREYK VVGRCLPTPK CRTPPLYRMR IFAPNHVVAK SRFWYFVSQL KKMKKSSGEI VYCGQVFEKS PLRVKNFGIW LRYDSRSGT HNMYREYRDL TTAGAVTQCY RDMGARHRAR AHSIQIMKVE EIAASKCRRP AVKQFHDSKI KFPLPHRVLR R QHKPRFTT KRPNTFF

+
Macromolecule #42: 60S ribosomal protein L21

MacromoleculeName: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 18.609988 KDa
SequenceString:
MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

+
Macromolecule #43: Ribosomal protein L22

MacromoleculeName: Ribosomal protein L22 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 11.836638 KDa
SequenceString:
QVLKFTLDCT HPVEDGIMDA ANFEQFLQER IKVNGKAGNL GGGVVTIERS KSKITVTSEV PFSKRYLKYL TKKYLKKNNL RDWLRVVAN SKESYELRYF QIN

+
Macromolecule #44: Ribosomal protein L23

MacromoleculeName: Ribosomal protein L23 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.892505 KDa
SequenceString:
MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

+
Macromolecule #45: Ribosomal protein L24

MacromoleculeName: Ribosomal protein L24 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.825111 KDa
SequenceString:
MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKAPTKAAPK QKIVKPVKVS APRVGGKR

UniProtKB: Large ribosomal subunit protein eL24

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Macromolecule #46: Ribosomal_L23eN domain-containing protein

MacromoleculeName: Ribosomal_L23eN domain-containing protein / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.768246 KDa
SequenceString:
MAPKAKKEAP APPKVEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

+
Macromolecule #48: Ribosomal protein L26

MacromoleculeName: Ribosomal protein L26 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.303363 KDa
SequenceString:
MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

+
Macromolecule #50: 60S ribosomal protein L27

MacromoleculeName: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 15.835831 KDa
SequenceString:
MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

UniProtKB: Large ribosomal subunit protein eL27

+
Macromolecule #51: 60S ribosomal protein L27a

MacromoleculeName: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 16.620561 KDa
SequenceString:
MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGMHH HRINFDKYHP GYFGKVGMRH YHLKRNQSFC PTVNLDKLWT LVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKG VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

+
Macromolecule #52: Nascent polypeptide-associated complex subunit alpha

MacromoleculeName: Nascent polypeptide-associated complex subunit alpha / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.406824 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQATTQQ AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVT GVTRVTIRKS KNILFVITKP DVYKSPASDT YIVFGEAKIE DLSQQAQLAA AEKFKVQGEA VSNIQENTQT P TVQEESEE ...String:
MPGEATETVP ATEQELPQPQ AETGSGTESD SDESVPELEE QDSTQATTQQ AQLAAAAEID EEPVSKAKQS RSEKKARKAM SKLGLRQVT GVTRVTIRKS KNILFVITKP DVYKSPASDT YIVFGEAKIE DLSQQAQLAA AEKFKVQGEA VSNIQENTQT P TVQEESEE EEVDETGVEV KDIELVMSQA NVSRAKAVRA LKNNSNDIVN AIMELTM

UniProtKB: Nascent polypeptide-associated complex subunit alpha

+
Macromolecule #53: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 53 / Number of copies: 97 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #54: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 54 / Number of copies: 5 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
GridModel: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec.
VitrificationCryogen name: ETHANE-PROPANE / Chamber humidity: 95 % / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.83 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 51843
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: MAXIMUM LIKELIHOOD

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