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- EMDB-1168: ERj1p uses a universal ribosomal adaptor site to coordinate the 8... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-1168 | |||||||||
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Title | ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane. | |||||||||
![]() | EM-volume of canine 80S-ribosome | |||||||||
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Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 23.0 Å | |||||||||
![]() | Blau M / Mullapudi S / Becker T / Dudek J / Zimmermann R / Penczek PA / Beckmann R | |||||||||
![]() | ![]() Title: ERj1p uses a universal ribosomal adaptor site to coordinate the 80S ribosome at the membrane. Authors: Michael Blau / Srinivas Mullapudi / Thomas Becker / Johanna Dudek / Richard Zimmermann / Pawel A Penczek / Roland Beckmann / ![]() Abstract: Ribosomes translating secretory and membrane proteins are targeted to the endoplasmic reticulum membrane and attach to the protein-conducting channel and ribosome-associated membrane proteins (RAMPs). ...Ribosomes translating secretory and membrane proteins are targeted to the endoplasmic reticulum membrane and attach to the protein-conducting channel and ribosome-associated membrane proteins (RAMPs). Recently, a new RAMP, ERj1p, has been identified that recruits BiP to ribosomes and regulates translational activity. Here we present the cryo-EM structure of a ribosome-ERj1p complex, revealing how ERj1p coordinates the ribosome at the membrane and how allosteric effects may mediate ERj1p's regulatory activity. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 10 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 6.1 KB 6.1 KB | Display Display | ![]() |
Images | ![]() | 43.5 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 235.3 KB | Display | ![]() |
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Full document | ![]() | 234.4 KB | Display | |
Data in XML | ![]() | 4.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | EM-volume of canine 80S-ribosome | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 3.5 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : canine ribosome
Entire | Name: canine ribosome |
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Components |
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-Supramolecule #1000: canine ribosome
Supramolecule | Name: canine ribosome / type: sample / ID: 1000 / Number unique components: 1 |
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-Supramolecule #1: canine ribosome
Supramolecule | Name: canine ribosome / type: complex / ID: 1 / Name.synonym: ribosome / Recombinant expression: No / Ribosome-details: ribosome-eukaryote: ALL |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | JEOL KYOTO-3000SFF |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Sample stage | Specimen holder: Gathan / Specimen holder model: GATAN LIQUID NITROGEN |
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Image processing
Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 23.0 Å |
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