EMDB-9703: Cryo-EM structure of the HCV IRES dependently initiated CMV-stall...

Basic information

• Entry: Database: EMDB / ID: EMD-9703
• Title: Cryo-EM structure of the HCV IRES dependently initiated CMV-stalled 80S ribosome (Structure iv)

Sample

Human 80S ribosome

• (nucleic-acidNucleic acid) x 7
• (60S ribosomal protein ...) x 41
• Ubiquitin-60S ribosomal protein L40
• (40S ribosomal protein ...) x 31
• Receptor of activated protein C kinase 1
• Ubiquitin-40S ribosomal protein S27a
• Eukaryotic peptide chain release factor subunit 1
• nascent peptide

Function / homology

Function:

Pink/Parkin Mediated Mitophagy / CLEC7A (Dectin-1) signaling / Dectin-1 mediated noncanonical NF-kB signaling / Hh mutants that don't undergo autocatalytic processing are degraded by ERAD / Hedgehog ligand biogenesis / TNFR1-induced NFkappaB signaling pathway / Regulation of TNFR1 signaling / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / Regulation of FZD by ubiquitination / Degradation of GLI2 by the proteasome / Degradation of DVL / Degradation of AXIN / Asymmetric localization of PCP proteins / AUF1 (hnRNP D0) binds and destabilizes mRNA / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / activated TAK1 mediates p38 MAPK activation / TAK1 activates NFkB by phosphorylation and activation of IKKs complex / Degradation of GLI1 by the proteasome / GLI3 is processed to GLI3R by the proteasome / ABC-family proteins mediated transport / Regulation of RAS by GAPs / MAP3K8 (TPL2)-dependent MAPK1/3 activation / Defective CFTR causes cystic fibrosis / NIK-->noncanonical NF-kB signaling / Regulation of necroptotic cell death / Negative regulation of MAPK pathway / TNFR2 non-canonical NF-kB pathway / RHO GTPases Activate Formins / Termination of translesion DNA synthesis / TNFR1-mediated ceramide production / Translesion synthesis by POLI / Translesion synthesis by POLK / Negative regulation of FGFR4 signaling / Negative regulation of FGFR3 signaling / Negative regulation of FGFR2 signaling / Negative regulation of FGFR1 signaling / Hedgehog 'on' state / Circadian Clock / Myoclonic epilepsy of Lafora / MAPK6/MAPK4 signaling / Downregulation of TGF-beta receptor signaling / Activated NOTCH1 Transmits Signal to the Nucleus / NOTCH1 Intracellular Domain Regulates Transcription / Regulation of activated PAK-2p34 by proteasome mediated degradation / NF-kB is activated and signals survival / p75NTR recruits signalling complexes / NRIF signals cell death from the nucleus / Downstream TCR signaling / Downregulation of SMAD2/3:SMAD4 transcriptional activity / TCF dependent signaling in response to WNT / Degradation of beta-catenin by the destruction complex / Viral mRNA Translation / SCF(Skp2)-mediated degradation of p27/p21 / EGFR downregulation / Vif-mediated degradation of APOBEC3G / Vpu mediated degradation of CD4 / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / Deactivation of the beta-catenin transactivating complex / Constitutive Signaling by NOTCH1 HD Domain Mutants / Autodegradation of the E3 ubiquitin ligase COP1 / Glycogen synthesis / RMTs methylate histone arginines / Regulation of innate immune responses to cytosolic DNA / NOTCH2 Activation and Transmission of Signal to the Nucleus / Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants / FCERI mediated NF-kB activation / Stimuli-sensing channels / Selenocysteine synthesis / Constitutive Signaling by NOTCH1 PEST Domain Mutants / Regulation of PLK1 Activity at G2/M Transition / Oncogene Induced Senescence / Senescence-Associated Secretory Phenotype (SASP) / Oxidative Stress Induced Senescence / Resolution of Sister Chromatid Cohesion / Separation of Sister Chromatids / HDR through Homologous Recombination (HRR) / UCH proteinases / APC-Cdc20 mediated degradation of Nek2A / Regulation of RUNX3 expression and activity / TICAM1-dependent activation of IRF3/IRF7 / NOTCH3 Activation and Transmission of Signal to the Nucleus / Regulation of expression of SLITs and ROBOs / ER Quality Control Compartment (ERQC) / Neddylation / Regulation of PTEN stability and activity / Regulation of PTEN localization / Regulation of RUNX2 expression and activity / Interleukin-1 signaling / RUNX1 regulates transcription of genes involved in differentiation of HSCs / Protein methylation / InlA-mediated entry of Listeria monocytogenes into host cells / InlB-mediated entry of Listeria monocytogenes into host cell / E3 ubiquitin ligases ubiquitinate target proteins / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Downregulation of ERBB2 signaling / TICAM1,TRAF6-dependent induction of TAK1 complex / Peroxisomal protein import / Cargo recognition for clathrin-mediated endocytosis / Negative regulation of NOTCH4 signaling

Domain/homology:

Ribosomal protein S19 conserved site / Ribosomal protein L10e/L16 / Ribosomal protein S5 domain 2-type fold, subgroup / Ribosomal protein L2, domain 2 / Ribosomal protein L2, domain 3 / WD40/YVTN repeat-like-containing domain superfamily / K homology domain-like, alpha/beta / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L30, ferredoxin-like fold domain / WD40-repeat-containing domain / Ribosomal protein S4e, central region / Ribosomal protein L30, conserved site / Ribosomal protein L34e, conserved site / Ribosomal protein S4, conserved site / Ribosomal S24e conserved site / Ribosomal S11, conserved site / Ribosomal protein S2, conserved site / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S6, eukaryotic / Ribosomal protein S4e, N-terminal / Ribosomal protein L10e, conserved site / TRASH domain / Ribosomal protein S30 / Ribosomal protein L41 / Ribosomal protein L34Ae / Translation protein SH3-like domain superfamily / Translation protein, beta-barrel domain superfamily / K homology domain superfamily, prokaryotic type / S15/NS1, RNA-binding / Ribosomal protein S13-like, H2TH / Ribosomal protein L37ae/L37e / Ribosomal protein S5, N-terminal / Zinc-binding ribosomal protein / Nucleic acid-binding, OB-fold / Ribosomal protein S13/S15, N-terminal / Nucleotide-binding alpha-beta plait domain superfamily / Ribosomal protein L23/L15e core domain superfamily / Ribosomal protein L30, N-terminal / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L25/L23 / Ribosomal protein L29, conserved site / Ribosomal protein L13e, conserved site / Ribosomal protein L7, eukaryotic / Ribosomal protein L15e, conserved site / Ribosomal protein L23 / Ribosomal protein L6, alpha-beta domain / Ribosomal protein L31e, conserved site / Ribosomal protein L39e, conserved site / G-protein beta WD-40 repeat / Ribosomal protein S5 domain 2-type fold / Ribosomal protein S9, conserved site / Ribosomal protein S7, conserved site / Ribosomal protein L5, conserved site / Ribosomal protein L14P, conserved site / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L18e/L15P / Ribosomal protein L18e, conserved site / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L2, C-terminal / Ribosomal protein L2, conserved site / Ribosomal protein S17, conserved site / Ubiquitin / Ribosomal protein L21e, conserved site / Ribosomal protein S17e, conserved site / Ribosomal protein L22/L17, conserved site / Ribosomal protein L27e, conserved site / Ribosomal protein L32e, conserved site / Ribosomal protein L35Ae, conserved site / Ribosomal protein L37e, conserved site / Ribosomal protein S10, conserved site / Ribosomal protein S13, conserved site / Ribosomal protein S14, conserved site / Ribosomal protein S19e, conserved site / Ubiquitin conserved site / Ribosomal protein S21e, conserved site / Ribosomal protein S3, conserved site / Ribosomal protein S3Ae, conserved site / Ribosomal protein S6e, conserved site / Ribosomal protein S8e, conserved site / Ribosomal protein L7Ae/L8/Nhp2 family / WD40 repeat, conserved site / Ribosomal protein L3, conserved site / Ribosomal protein S12/S23 / Ribosomal protein L24/L26, conserved site / Ribosomal protein L5 domain superfamily / Ribosomal protein L13e / Ribosomal protein L21e / Ribosomal protein L15, conserved site / Ribosomal protein L10e / Ribosomal protein S14 / Ribosomal protein S17e / Ribosomal protein S19e / Ribosomal protein S3, C-terminal / Ribosomal protein S6e / Ribosomal protein L32e / Ribosomal protein L22/L17 / Ribosomal protein L37e

Component:

40S ribosomal protein S6 / 40S ribosomal protein S28 / 40S ribosomal protein S26 / 40S ribosomal protein S25 / 40S ribosomal protein S24 / 40S ribosomal protein S15 / 60S ribosomal protein L23 / 40S ribosomal protein S29 / 60S ribosomal protein L23a / 40S ribosomal protein S4, X isoform / Eukaryotic peptide chain release factor subunit 1 / 60S ribosomal protein L7a / 40S ribosomal protein S11 / 40S ribosomal protein S13 / 40S ribosomal protein S18 / 60S ribosomal protein L30 / 40S ribosomal protein S30 / 60S ribosomal protein L18 / 60S ribosomal protein L39 / Receptor of activated protein C kinase 1 / 60S ribosomal protein L10-like / 40S ribosomal protein S14 / 60S ribosomal protein L6 / 60S ribosomal protein L18a / 60S ribosomal protein L19 / 60S ribosomal protein L36a / 60S ribosomal protein L24 / 40S ribosomal protein S21 / 60S ribosomal protein L31 / 60S ribosomal protein L38 / Ubiquitin-60S ribosomal protein L40 / Ubiquitin-40S ribosomal protein S27a / 60S ribosomal protein L41 / 60S ribosomal protein L8 / 60S ribosomal protein L11 / 60S ribosomal protein L32 / 40S ribosomal protein S23 / 40S ribosomal protein S9 / 40S ribosomal protein S16 / 60S ribosomal protein L13 / 60S ribosomal protein L13a / 60S ribosomal protein L3 / 40S ribosomal protein S19 / 60S ribosomal protein L4 / 60S ribosomal protein L22 / 60S ribosomal protein L9 / 40S ribosomal protein S12 / 60S ribosomal protein L35 / 40S ribosomal protein S3 / 60S ribosomal protein L17 / 60S ribosomal protein L7 / 60S ribosomal protein L35a / 40S ribosomal protein S2 / 40S ribosomal protein SA / 40S ribosomal protein S17 / 40S ribosomal protein S27 / 60S ribosomal protein L27a / 40S ribosomal protein S15a / 40S ribosomal protein S3a / 40S ribosomal protein S8 / 40S ribosomal protein S7 / 60S ribosomal protein L37 / 60S ribosomal protein L37a / 60S ribosomal protein L27 / 60S ribosomal protein L15 / 60S ribosomal protein L26 / 40S ribosomal protein S20 / 60S ribosomal protein L5 / 60S ribosomal protein L14 / 60S ribosomal protein L34 / 60S ribosomal protein L29 / 40S ribosomal protein S10 / 40S ribosomal protein S5 / 60S ribosomal protein L28 / 60S ribosomal protein L21 / 60S ribosomal protein L36

• Biological species: Homo sapiens (human) / Human (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.9 Å
• Authors: Yokoyama T / Shigematsu H / Shirouzu M / Imataka H / Ito T
• Citation: Journal: Mol. Cell / Year: 2019; Title: HCV IRES Captures an Actively Translating 80S Ribosome.; Authors: Takeshi Yokoyama / Kodai Machida / Wakana Iwasaki / Tomoaki Shigeta / Madoka Nishimoto / Mari Takahashi / Ayako Sakamoto / Mayumi Yonemochi / Yoshie Harada / Hideki Shigematsu / Mikako Shirouzu / Hisashi Tadakuma / Hiroaki Imataka / Takuhiro Ito / ; Abstract: Translation initiation of hepatitis C virus (HCV) genomic RNA is induced by an internal ribosome entry site (IRES). Our cryoelectron microscopy (cryo-EM) analysis revealed that the HCV IRES binds to the solvent side of the 40S platform of the cap-dependently translating 80S ribosome. Furthermore, we obtained the cryo-EM structures of the HCV IRES capturing the 40S subunit of the IRES-dependently translating 80S ribosome. In the elucidated structures, the HCV IRES "body," consisting of domain III except for subdomain IIIb, binds to the 40S subunit, while the "long arm," consisting of domain II, remains flexible and does not impede the ongoing translation. Biochemical experiments revealed that the cap-dependently translating ribosome becomes a better substrate for the HCV IRES than the free ribosome. Therefore, the HCV IRES is likely to efficiently induce the translation initiation of its downstream mRNA with the captured translating ribosome as soon as the ongoing translation terminates.
• Validation Report: PDB-ID: 6ip8; ; SummaryFull reportAbout validation report
• Date: Deposition: Nov 2, 2018 / Header (metadata) release: May 29, 2019 / Map release: May 29, 2019 / Update: May 29, 2019

Map

• File: Download / File: emd_9703.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.49 Å

Density

Contour Level	By AUTHOR: 0.05 / Movie #1: 0.05
Minimum - Maximum	-0.14547415 - 0.26877546
Average (Standard dev.)	0.00017937103 (±0.013128813)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 420 420 420 Spacing 420 420 420
Cell	A=B=C: 625.8 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.49	1.49	1.49
M x/y/z	420	420	420
origin x/y/z	0.000	0.000	0.000
length x/y/z	625.800	625.800	625.800
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	420	420	420
D min/max/mean	-0.145	0.269	0.000

Supplemental data

Sample components

Entire Human 80S ribosome

• Entire: Name: Human 80S ribosome / Number of components: 85

Component #1: protein, Human 80S ribosome

• Protein: Name: Human 80S ribosome / Recombinant expression: No
• Source: Species: Homo sapiens (human)

Component #2: nucleic-acid, 28S ribosomal RNA

nucleic acid

Name: 28S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAACG GCGAGUGAAC AGGGAAGAGC CCAGCGCCGA AUCCCCGCCC CGCGGCGGGG CGCGGGACAU GUGGCGUACG GAAGACCCGC UCCCCGGCGC CGCUCGUGGG GGGCCCAAGU CCUUCUGAUC GAGGCCCAGC CCGUGGACGG UGUGAGGCCG GUAGCGGCCC CCGGCGCGCC GGGCCCGGGU CUUCCCGGAG UCGGGUUGCU UGGGAAUGCA GCCCAAAGCG GGUGGUAAAC UCCAUCUAAG GCUAAAUACC GGCACGAGAC CGAUAGUCAA CAAGUACCGU AAGGGAAAGU UGAAAAGAAC UUUGAAGAGA GAGUUCAAGA GGGCGUGAAA CCGUUAAGAG GUAAACGGGU GGGGUCCGCG CAGUCCGCCC GGAGGAUUCA ACCCGGCGGC GGGUCCGGCC GUGUCGGCGG CCCGGCGGAU CUUUCCCGCC CCCCGUUCCU CCCGACCCCU CCACCCGCCC UCCCUUCCCC CGCCGCCCCU CCUCCUCCUC CCCGGAGGGG GCGGGCUCCG GCGGGUGCGG GGGUGGGCGG GCGGGGCCGG GGGUGGGGUC GGCGGGGGAC CGUCCCCCGA CCGGCGACCG GCCGCCGCCG GGCGCAUUUC CACCGCGGCG GUGCGCCGCG ACCGGCUCCG GGACGGCUGG GAAGGCCCGG CGGGGAAGGU GGCUCGGGGG GCCCCGUCCG UCCGUCCGUC CGUCCUCCUC CUCCCCCGUC UCCGCCCCCC GGCCCCGCGU CCUCCCUCGG GAGGGCGCGC GGGUCGGGGC GGCGGCGGCG GCGGCGGUGG CGGCGGCGGC GGCGGCGGCG GGACCGAAAC CCCCCCCGAG UGUUACAGCC CCCCCGGCAG CAGCACUCGC CGAAUCCCGG GGCCGAGGGA GCGAGACCCG UCGCCGCGCU CUCCCCCCUC CCGGCGCCCA CCCCCGCGGG GAAUCCCCCG CGAGGGGGGU CUCCCCCGCG GGGGCGCGCC GGCGUCUCCU CGUGGGGGGG CCGGGCCACC CCUCCCACGG CGCGACCGCU CUCCCACCCC UCCUCCCCGC GCCCCCGCCC CGGCGACGGG GGGGGUGCCG CGCGCGGGUC GGGGGGCGGG GCGGACUGUC CCCAGUGCGC CCCGGGCGGG UCGCGCCGUC GGGCCCGGGG GAGGUUCUCU CGGGGCCACG CGCGCGUCCC CCGAAGAGGG GGACGGCGGA GCGAGCGCAC GGGGUCGGCG GCGACGUCGG CUACCCACCC GACCCGUCUU GAAACACGGA CCAAGGAGUC UAACACGUGC GCGAGUCGGG GGCUCGCACG AAAGCCGCCG UGGCGCAAUG AAGGUGAAGG CCGGCGCGCU CGCCGGCCGA GGUGGGAUCC CGAGGCCUCU CCAGUCCGCC GAGGGCGCAC CACCGGCCCG UCUCGCCCGC CGCGCCGGGG AGGUGGAGCA CGAGCGCACG UGUUAGGACC CGAAAGAUGG UGAACUAUGC CUGGGCAGGG CGAAGCCAGA GGAAACUCUG GUGGAGGUCC GUAGCGGUCC UGACGUGCAA AUCGGUCGUC CGACCUGGGU AUAGGGGCGA AAGACUAAUC GAACCAUCUA GUAGCUGGUU CCCUCCGAAG UUUCCCUCAG GAUAGCUGGC GCUCUCGCAG ACCCGACGCA CCCCCGCCAC GCAGUUUUAU CCGGUAAAGC GAAUGAUUAG AGGUCUUGGG GCCGAAACGA UCUCAACCUA UUCUCAAACU UUAAAUGGGU AAGAAGCCCG GCUCGCUGGC GUGGAGCCGG GCGUGGAAUG CGAGUGCCUA GUGGGCCACU UUUGGUAAGC AGAACUGGCG CUGCGGGAUG AACCGAACGC CGGGUUAAGG CGCCCGAUGC CGACGCUCAU CAGACCCCAG AAAAGGUGUU GGUUGAUAUA GACAGCAGGA CGGUGGCCAU GGAAGUCGGA AUCCGCUAAG GAGUGUGUAA CAACUCACCU GCCGAAUCAA CUAGCCCUGA AAAUGGAUGG CGCUGGAGCG UCGGGCCCAU ACCCGGCCGU CGCCGGCAGU CGAGAGUGGA CGGGAGCGGC GGCGGCGGCG CGCGCGCGCG CGCGUGUGGU GUGCGUCGGA GGGCGGCGGC GGCGGCGGCG GCGGGGGUGU GGGGUCCUUC CCCCGCCCCC CCCCCCACGC CUCCUCCCCU CCUCCCGCCC ACGCCCCGCU CCCCGCCCCC GGAGCCCCGC GGACGCUACG CCGCGACGAG UAGGAGGGCC GCUGCGGUGA GCCUUGAAGC CUAGGGCGCG GGCCCGGGUG GAGCCGCCGC AGGUGCAGAU CUUGGUGGUA GUAGCAAAUA UUCAAACGAG AACUUUGAAG GCCGAAGUGG AGAAGGGUUC CAUGUGAACA GCAGUUGAAC AUGGGUCAGU CGGUCCUGAG AGAUGGGCGA GCGCCGUUCC GAAGGGACGG GCGAUGGCCU CCGUUGCCCU CGGCCGAUCG AAAGGGAGUC GGGUUCAGAU CCCCGAAUCC GGAGUGGCGG AGAUGGGCGC CGCGAGGCGU CCAGUGCGGU AACGCGACCG AUCCCGGAGA AGCCGGCGGG AGCCCCGGGG AGAGUUCUCU UUUCUUUGUG AAGGGCAGGG CGCCCUGGAA UGGGUUCGCC CCGAGAGAGG GGCCCGUGCC UUGGAAAGCG UCGCGGUUCC GGCGGCGUCC GGUGAGCUCU CGCUGGCCCU UGAAAAUCCG GGGGAGAGGG UGUAAAUCUC GCGCCGGGCC GUACCCAUAU CCGCAGCAGG UCUCCAAGGU GAACAGCCUC UGGCAUGUUG GAACAAUGUA GGUAAGGGAA GUCGGCAAGC CGGAUCCGUA ACUUCGGGAU AAGGAUUGGC UCUAAGGGCU GGGUCGGUCG GGCUGGGGCG CGAAGCGGGG CUGGGCGCGC GCCGCGGCUG GACGAGGCGC CGCCGCCCCC CCCACGCCCG GGGCACCCCC CUCGCGGCCC UCCCCCGCCC CACCCCGCGC GCGCCGCUCG CUCCCUCCCC GCCCCGCGCC CUCUCUCUCU CUCUCUCCCC CGCUCCCCGU CCUCCCCCCU CCCCGGGGGA GCGCCGCGUG GGGGCGGCGG CGGGGGGAGA AGGGUCGGGG CGGCAGGGGC CGGCGGCGGC CCGCCGCGGG GCCCCGGCGG CGGGGGCACG GUCCCCCGCG AGGGGGGCCC GGGCACCCGG GGGGCCGGCG GCGGCGGCGA CUCUGGACGC GAGCCGGGCC CUUCCCGUGG AUCGCCCCAG CUGCGGCGGG CGUCGCGGCC GCCCCCGGGG AGCCCGGCGG GCGCCGGCGC GCCCCCCCCC CCACCCCACG UCUCGUCGCG CGCGCGUCCG CUGGGGGCGG GGAGCGGUCG GGCGGCGGCG GUCGGCGGGC GGCGGGGCGG GGCGGUUCGU CCCCCCGCCC UACCCCCCCG GCCCCGUCCG CCCCCCGUUC CCCCCUCCUC CUCGGCGCGC GGCGGCGGCG GCGGCAGGCG GCGGAGGGGC CGCGGGCCGG UCCCCCCCGC CGGGUCCGCC CCCGGGGCCG CGGUUCCGCG CGGCGCCUCG CCUCGGCCGG CGCCUAGCAG CCGACUUAGA ACUGGUGCGG ACCAGGGGAA UCCGACUGUU UAAUUAAAAC AAAGCAUCGC GAAGGCCCGC GGCGGGUGUU GACGCGAUGU GAUUUCUGCC CAGUGCUCUG AAUGUCAAAG UGAAGAAAUU CAAUGAAGCG CGGGUAAACG GCGGGAGUAA CUAUGACUCU CUUAAGGUAG CCAAAUGCCU CGUCAUCUAA UUAGUGACGC GCAUGAAUGG AUGAACGAGA UUCCCACUGU CCCUACCUAC UAUCCAGCGA AACCACAGCC AAGGGAACGG GCUUGGCGGA AUCAGCGGGG AAAGAAGACC CUGUUGAGCU UGACUCUAGU CUGGCACGGU GAAGAGACAU GAGAGGUGUA GAAUAAGUGG GAGGCCCCCG GCGCCCCCCC GGUGUCCCCG CGAGGGGCCC GGGGCGGGGU CCGCCGGCCC UGCGGGCCGC CGGUGAAAUA CCACUACUCU GAUCGUUUUU UCACUGACCC GGUGAGGCGG GGGGGCGAGC CCCGAGGGGC UCUCGCUUCU GGCGCCAAGC GCCCGGCCGC GCGCCGGCCG GGCGCGACCC GCUCCGGGGA CAGUGCCAGG UGGGGAGUUU GACUGGGGCG GUACACCUGU CAAACGGUAA CGCAGGUGUC CUAAGGCGAG CUCAGGGAGG ACAGAAACCU CCCGUGGAGC AGAAGGGCAA AAGCUCGCUU GAUCUUGAUU UUCAGUACGA AUACAGACCG UGAAAGCGGG GCCUCACGAU CCUUCUGACC UUUUGGGUUU UAAGCAGGAG GUGUCAGAAA AGUUACCACA GGGAUAACUG GCUUGUGGCG GCCAAGCGUU CAUAGCGACG UCGCUUUUUG AUCCUUCGAU GUCGGCUCUU CCUAUCAUUG UGAAGCAGAA UUCACCAAGC GUUGGAUUGU UCACCCACUA AUAGGGAACG UGAGCUGGGU UUAGACCGUC GUGAGACAGG UUAGUUUUAC CCUACUGAUG AUGUGUUGUU GCCAUGGUAA UCCUGCUCAG UACGAGAGGA ACCGCAGGUU CAGACAUUUG GUGUAUGUGC UUGGCUGAGG AGCCAAUGGG GCGAAGCUAC CAUCUGUGGG AUUAUGACUG AACGCCUCUA AGUCAGAAUC CCGCCCAGGC GGAACGAUAC GGCAGCGCCG CGGAGCCUCG GUUGGCCUCG GAUAGCCGGU CCCCCGCCUG UCCCCGCCGG CGGGCCGCCC CCCCCCUCCA CGCGCCCCGC GCGCGCGGGA GGGCGCGUGC CCCGCCGCGC GCCGGGACCG GGGUCCGGUG CGGAGUGCCC UUCGUCCUGG GAAACGGGGC GCGGCCGGAG AGGCGGCCGC CCCCUCGCCC GUCACGCACC GCACGUUCGU GGGGAACCUG GCGCUAAACC AUUCGUAGAC GACCUGCUUC UGGGUCGGGG UUUCGUACGU AGCAGAGCAG CUCCCUCGCU GCGAUCUAUU GAAAGUCAGC CCUCGACACA AGGGUUUGUC

• Mass: Theoretical: 1.640182 MDa
• Source: Species: Human (human)

Component #3: nucleic-acid, 5S ribosomal RNA

nucleic acid

Name: 5S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:

GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAGA CCGCCUGGGA AUACCGGGUG CUGUAGGCUU U

• Mass: Theoretical: 38.998078 kDa
• Source: Species: Human (human)

Component #4: nucleic-acid, 5.8S ribosomal RNA

nucleic acid

Name: 5.8S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAUC AUCGACACUU CGAACGCACU UGCGGCCCCG GGUUCCUCCC GGGGCUACGC CUGUCUGAGC GUCGCUU

• Mass: Theoretical: 50.449812 kDa
• Source: Species: Human (human)

Component #5: protein, 60S ribosomal protein L8

• Protein: Name: 60S ribosomal protein L8 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 28.088863 kDa
• Source: Species: Human (human)

Component #6: protein, 60S ribosomal protein L3

• Protein: Name: 60S ribosomal protein L3 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 46.211113 kDa
• Source: Species: Human (human)

Component #7: protein, 60S ribosomal protein L4

• Protein: Name: 60S ribosomal protein L4 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 47.804621 kDa
• Source: Species: Human (human)

Component #8: protein, 60S ribosomal protein L5

• Protein: Name: 60S ribosomal protein L5 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 34.426789 kDa
• Source: Species: Human (human)

Component #9: protein, 60S ribosomal protein L6

• Protein: Name: 60S ribosomal protein L6 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 32.810176 kDa
• Source: Species: Human (human)

Component #10: protein, 60S ribosomal protein L7

• Protein: Name: 60S ribosomal protein L7 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 29.290973 kDa
• Source: Species: Human (human)

Component #11: protein, 60S ribosomal protein L7a

• Protein: Name: 60S ribosomal protein L7a / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 30.061785 kDa
• Source: Species: Human (human)

Component #12: protein, 60S ribosomal protein L9

• Protein: Name: 60S ribosomal protein L9 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 21.899471 kDa
• Source: Species: Human (human)

Component #13: protein, 60S ribosomal protein L10-like

• Protein: Name: 60S ribosomal protein L10-like / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 24.570949 kDa
• Source: Species: Human (human)

Component #14: protein, 60S ribosomal protein L11

• Protein: Name: 60S ribosomal protein L11 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 20.288465 kDa
• Source: Species: Human (human)

Component #15: protein, 60S ribosomal protein L13

• Protein: Name: 60S ribosomal protein L13 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 24.321682 kDa
• Source: Species: Human (human)

Component #16: protein, 60S ribosomal protein L14

• Protein: Name: 60S ribosomal protein L14 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 23.485016 kDa
• Source: Species: Human (human)

Component #17: protein, 60S ribosomal protein L15

• Protein: Name: 60S ribosomal protein L15 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 24.207285 kDa
• Source: Species: Human (human)

Component #18: protein, 60S ribosomal protein L13a

• Protein: Name: 60S ribosomal protein L13a / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 23.633412 kDa
• Source: Species: Human (human)

Component #19: protein, 60S ribosomal protein L17

• Protein: Name: 60S ribosomal protein L17 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 21.44317 kDa
• Source: Species: Human (human)

Component #20: protein, 60S ribosomal protein L18

• Protein: Name: 60S ribosomal protein L18 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 21.687676 kDa
• Source: Species: Human (human)

Component #21: protein, 60S ribosomal protein L19

• Protein: Name: 60S ribosomal protein L19 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 23.535281 kDa
• Source: Species: Human (human)

Component #22: protein, 60S ribosomal protein L18a

• Protein: Name: 60S ribosomal protein L18a / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 20.808514 kDa
• Source: Species: Human (human)

Component #23: protein, 60S ribosomal protein L21

• Protein: Name: 60S ribosomal protein L21 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 18.609988 kDa
• Source: Species: Human (human)

Component #24: protein, 60S ribosomal protein L22

• Protein: Name: 60S ribosomal protein L22 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 14.813015 kDa
• Source: Species: Human (human)

Component #25: protein, 60S ribosomal protein L23

• Protein: Name: 60S ribosomal protein L23 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 14.892505 kDa
• Source: Species: Human (human)

Component #26: protein, 60S ribosomal protein L24

• Protein: Name: 60S ribosomal protein L24 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 17.825111 kDa
• Source: Species: Human (human)

Component #27: protein, 60S ribosomal protein L23a

• Protein: Name: 60S ribosomal protein L23a / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 17.740193 kDa
• Source: Species: Human (human)

Component #28: protein, 60S ribosomal protein L26

• Protein: Name: 60S ribosomal protein L26 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 17.303363 kDa
• Source: Species: Human (human)

Component #29: protein, 60S ribosomal protein L27

• Protein: Name: 60S ribosomal protein L27 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 15.835831 kDa
• Source: Species: Human (human)

Component #30: protein, 60S ribosomal protein L27a

• Protein: Name: 60S ribosomal protein L27a / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 16.604535 kDa
• Source: Species: Human (human)

Component #31: protein, 60S ribosomal protein L29

• Protein: Name: 60S ribosomal protein L29 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 17.804275 kDa
• Source: Species: Human (human)

Component #32: protein, 60S ribosomal protein L30

• Protein: Name: 60S ribosomal protein L30 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 12.805092 kDa
• Source: Species: Human (human)

Component #33: protein, 60S ribosomal protein L31

• Protein: Name: 60S ribosomal protein L31 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 14.494938 kDa
• Source: Species: Human (human)

Component #34: protein, 60S ribosomal protein L32

• Protein: Name: 60S ribosomal protein L32 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 15.898932 kDa
• Source: Species: Human (human)

Component #35: protein, 60S ribosomal protein L35a

• Protein: Name: 60S ribosomal protein L35a / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 12.564743 kDa
• Source: Species: Human (human)

Component #36: protein, 60S ribosomal protein L34

• Protein: Name: 60S ribosomal protein L34 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 13.326074 kDa
• Source: Species: Human (human)

Component #37: protein, 60S ribosomal protein L35

• Protein: Name: 60S ribosomal protein L35 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 14.593624 kDa
• Source: Species: Human (human)

Component #38: protein, 60S ribosomal protein L36

• Protein: Name: 60S ribosomal protein L36 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 12.290859 kDa
• Source: Species: Human (human)

Component #39: protein, 60S ribosomal protein L37

• Protein: Name: 60S ribosomal protein L37 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 11.111032 kDa
• Source: Species: Human (human)

Component #40: protein, 60S ribosomal protein L38

• Protein: Name: 60S ribosomal protein L38 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 8.238948 kDa
• Source: Species: Human (human)

Component #41: protein, 60S ribosomal protein L39

• Protein: Name: 60S ribosomal protein L39 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 6.426759 kDa
• Source: Species: Human (human)

Component #42: protein, Ubiquitin-60S ribosomal protein L40

• Protein: Name: Ubiquitin-60S ribosomal protein L40 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 14.758394 kDa
• Source: Species: Human (human)

Component #43: protein, 60S ribosomal protein L41

• Protein: Name: 60S ribosomal protein L41 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 3.473451 kDa
• Source: Species: Human (human)

Component #44: protein, 60S ribosomal protein L36a

• Protein: Name: 60S ribosomal protein L36a / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 12.476973 kDa
• Source: Species: Human (human)

Component #45: protein, 60S ribosomal protein L37a

• Protein: Name: 60S ribosomal protein L37a / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 10.29935 kDa
• Source: Species: Human (human)

Component #46: protein, 60S ribosomal protein L28

• Protein: Name: 60S ribosomal protein L28 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 15.784622 kDa
• Source: Species: Human (human)

Component #47: nucleic-acid, 18S ribosomal RNA

nucleic acid

Name: 18S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:

UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGCG AAUGGCUCAU UAAAUCAGUU AUGGUUCCUU UGGUCGCUCG CUCCUCUCCC ACUUGGAUAA CUGUGGUAAU UCUAGAGCUA AUACAUGCCG ACGGGCGCUG ACCCCCUUCG CGGGGGGGAU GCGUGCAUUU AUCAGAUCAA AACCAACCCG GUCAGCCCCU CUCCGGCCCC GGCCGGGGGG CGGGCGCCGG CGGCUUUGGU GACUCUAGAU AACCUCGGGC CGAUCGCACG CCCCCCGUGG CGGCGACGAC CCAUUCGAAC GUCUGCCCUA UCAACUUUCG AUGGUAGUCG CCGUGCCUAC CAUGGUGACC ACGGGUGACG GGGAAUCAGG GUUCGAUUCC GGAGAGGGAG CCUGAGAAAC GGCUACCACA UCCAAGGAAG GCAGCAGGCG CGCAAAUUAC CCACUCCCGA CCCGGGGAGG UAGUGACGAA AAAUAACAAU ACAGGACUCU UUCGAGGCCC UGUAAUUGGA AUGAGUCCAC UUUAAAUCCU UCCGCGAGGA UCCAUUGGAG GGCAAGUCUG GUGCCAGCAG CCGCGGUAAU UCCAGCUCCA AUAGCGUAUA UUAAAGUUGC UGCAGUUAAA AAGCUCGUAG UUGGAUCUUG GGAGCGGGCG GGCGGUCCGC CGCGAGGCGA GCCACCGCCC GUCCCCGCCC CUUGCCUCUC GGCGCCCCCU CGAUGCUCUU AGCUGAGUGU CCCGCGGGGC CCGAAGCAUU UACUUUGAAA AAAUUAGAGU GUUCAAAGCA GGCCCGAGCC GCCUGGAUAC CGCAGCUAGG AAUAAUGGAA UAGGACCGCG GUUCUAUUUU GUUGGUUUUC GGAACUGAGG CCAUGAUUAA GAGGGACGGC CGGGGGCAUU CGUAUUGCGC CGCUAGAGGU GAAAUUCUUG GACCGGCGCA AGACGGACCA GAGCGAAAGC AUUUGCCAAG AAUGUUUUCA UUAAUCAAGA ACGAAAGUCG GAGGUUCGAA GACGAUCAGA UACCGUCGUA GUUCCGACCA UAAACGAUGC CGACUGGCGA UGCGGCGGCG UUAUUCCCAU GACCCGCCGG GCAGCUUCCG GGAAACCAAA GUCUUUGGGU UCCGGGGGGA GUAUGGUUGC AAAGCUGAAA CUUAAAGGAA UUGACGGAAG GGCACCACCA GGAGUGGAGC CUGCGGCUUA AUUUGACUCA ACACGGGAAA CCUCACCCGG CCCGGACACG GACAGGAUUG ACAGAUUGAU AGCUCUUUCU CGAUUCCGUG GGUGGUGGUG CAUGGCCGUU CUUAGUUGGU GGAGCGAUUU GUCUGGUUAA UUCCGAUAAC GAACGAGACU CUGGCAUGCU AACUAGUUAC GCGACCCCCG AGCGGUCGGC GUCCCCCAAC UUCUUAGAGG GACAAGUGGC GUUCAGCCAC CCGAGAUUGA GCAAUAACAG GUCUGUGAUG CCCUUAGAUG UCCGGGGCUG CACGCGCGCU ACACUGACUG GCUCAGCGUG UGCCUACCCU ACGCCGGCAG GCGCGGGUAA CCCGUUGAAC CCCAUUCGUG AUGGGGAUCG GGGAUUGCAA UUAUUCCCCA UGAACGAGGA AUUCCCAGUA AGUGCGGGUC AUAAGCUUGC GUUGAUUAAG UCCCUGCCCU UUGUACACAC CGCCCGUCGC UACUACCGAU UGGAUGGUUU AGUGAGGCCC UCGGAUCGGC CCCGCCGGGG UCGGCCCACG GCCCUGGCGG AGCGCUGAGA AGACGGUCGA ACUUGACUAU CUAGAGGAAG UAAAAGUCGU AACAAGGUUU CCGUAGGUGA ACCUGCGGAA GGAUCAUUA

• Mass: Theoretical: 602.752875 kDa
• Source: Species: Human (human)

Component #48: protein, 40S ribosomal protein SA

• Protein: Name: 40S ribosomal protein SA / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 32.883938 kDa
• Source: Species: Human (human)

Component #49: protein, 40S ribosomal protein S3a

• Protein: Name: 40S ribosomal protein S3a / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 30.002061 kDa
• Source: Species: Human (human)

Component #50: protein, 40S ribosomal protein S3

• Protein: Name: 40S ribosomal protein S3 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 26.729369 kDa
• Source: Species: Human (human)

Component #51: protein, 40S ribosomal protein S4, X isoform

• Protein: Name: 40S ribosomal protein S4, X isoformRibosome / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 29.654869 kDa
• Source: Species: Human (human)

Component #52: protein, 40S ribosomal protein S5

• Protein: Name: 40S ribosomal protein S5 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 22.913453 kDa
• Source: Species: Human (human)

Component #53: protein, 40S ribosomal protein S7

• Protein: Name: 40S ribosomal protein S7 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 22.168914 kDa
• Source: Species: Human (human)

Component #54: protein, 40S ribosomal protein S8

• Protein: Name: 40S ribosomal protein S8 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 24.263387 kDa
• Source: Species: Human (human)

Component #55: protein, 40S ribosomal protein S10

• Protein: Name: 40S ribosomal protein S10 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 18.933846 kDa
• Source: Species: Human (human)

Component #56: protein, 40S ribosomal protein S11

• Protein: Name: 40S ribosomal protein S11 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 18.468826 kDa
• Source: Species: Human (human)

Component #57: protein, 40S ribosomal protein S15

• Protein: Name: 40S ribosomal protein S15 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 17.076207 kDa
• Source: Species: Human (human)

Component #58: protein, 40S ribosomal protein S16

• Protein: Name: 40S ribosomal protein S16 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 16.477377 kDa
• Source: Species: Human (human)

Component #59: protein, 40S ribosomal protein S17

• Protein: Name: 40S ribosomal protein S17 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 15.578156 kDa
• Source: Species: Human (human)

Component #60: protein, 40S ribosomal protein S18

• Protein: Name: 40S ribosomal protein S18 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 17.759777 kDa
• Source: Species: Human (human)

Component #61: protein, 40S ribosomal protein S19

• Protein: Name: 40S ribosomal protein S19 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 16.091562 kDa
• Source: Species: Human (human)

Component #62: protein, 40S ribosomal protein S20

• Protein: Name: 40S ribosomal protein S20 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 13.398763 kDa
• Source: Species: Human (human)

Component #63: protein, 40S ribosomal protein S21

• Protein: Name: 40S ribosomal protein S21 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 9.124389 kDa
• Source: Species: Human (human)

Component #64: protein, 40S ribosomal protein S23

• Protein: Name: 40S ribosomal protein S23 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 15.844666 kDa
• Source: Species: Human (human)

Component #65: protein, 40S ribosomal protein S26

• Protein: Name: 40S ribosomal protein S26 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 13.047532 kDa
• Source: Species: Human (human)

Component #66: protein, 40S ribosomal protein S28

• Protein: Name: 40S ribosomal protein S28 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 7.855052 kDa
• Source: Species: Human (human)

Component #67: protein, 40S ribosomal protein S29

• Protein: Name: 40S ribosomal protein S29 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 6.690821 kDa
• Source: Species: Human (human)

Component #68: protein, Receptor of activated protein C kinase 1

• Protein: Name: Receptor of activated protein C kinase 1 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 35.115652 kDa
• Source: Species: Human (human)

Component #69: protein, 40S ribosomal protein S2

• Protein: Name: 40S ribosomal protein S2 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 31.376516 kDa
• Source: Species: Human (human)

Component #70: protein, 40S ribosomal protein S6

• Protein: Name: 40S ribosomal protein S6 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 28.751906 kDa
• Source: Species: Human (human)

Component #71: protein, 40S ribosomal protein S9

• Protein: Name: 40S ribosomal protein S9 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 22.641564 kDa
• Source: Species: Human (human)

Component #72: protein, 40S ribosomal protein S12

• Protein: Name: 40S ribosomal protein S12 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 14.548896 kDa
• Source: Species: Human (human)

Component #73: protein, 40S ribosomal protein S13

• Protein: Name: 40S ribosomal protein S13 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 17.259389 kDa
• Source: Species: Human (human)

Component #74: protein, 40S ribosomal protein S14

• Protein: Name: 40S ribosomal protein S14 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 16.302772 kDa
• Source: Species: Human (human)

Component #75: protein, 40S ribosomal protein S15a

• Protein: Name: 40S ribosomal protein S15a / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 14.865555 kDa
• Source: Species: Human (human)

Component #76: protein, 40S ribosomal protein S24

• Protein: Name: 40S ribosomal protein S24 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 15.463333 kDa
• Source: Species: Human (human)

Component #77: protein, 40S ribosomal protein S25

• Protein: Name: 40S ribosomal protein S25 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 13.776224 kDa
• Source: Species: Human (human)

Component #78: protein, 40S ribosomal protein S27

• Protein: Name: 40S ribosomal protein S27 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 9.480186 kDa
• Source: Species: Human (human)

Component #79: protein, 40S ribosomal protein S30

• Protein: Name: 40S ribosomal protein S30 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 6.668938 kDa
• Source: Species: Human (human)

Component #80: protein, Ubiquitin-40S ribosomal protein S27a

• Protein: Name: Ubiquitin-40S ribosomal protein S27a / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 18.004041 kDa
• Source: Species: Human (human)

Component #81: nucleic-acid, mRNA

nucleic acid

Name: mRNAMessenger RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:

CCGCCUUAAC UC

• Mass: Theoretical: 3.708248 kDa
• Source: Species: Human (human)

Component #82: protein, Eukaryotic peptide chain release factor subunit 1

• Protein: Name: Eukaryotic peptide chain release factor subunit 1 / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 48.476105 kDa
• Source: Species: Human (human)

Component #83: protein, nascent peptide

• Protein: Name: nascent peptide / Number of Copies: 1 / Recombinant expression: No
• Mass: Theoretical: 1.865305 kDa
• Source: Species: Human (human)

Component #84: nucleic-acid, P-site tRNA

nucleic acid

Name: P-site tRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:

GGCUCGUUGG UCUAGGGGUA UGAUUCUCGC UUAGGGUGCG AGAGGUCCCG GGUUCAAAUC CCGGACGAGC CCCCA

• Mass: Theoretical: 24.189314 kDa
• Source: Species: Human (human)

Component #85: nucleic-acid, HCV-IRES RNA

nucleic acid

Name: HCV-IRES RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:

CUCCCCUGUG AGGAACUACU GUCUUCACGC AGAAAGCGUC UAGCCAUGGC GUUAGUAUGA GUGUCGUGCA GCCUCCAGGA CCCCCCCUCC CGGGAGAGCC AUAGUGGUCU GCGGAACCGG UGAGUACACC GGAAUUGCCA GGACGACCGG GUCCUUUCUU GGAUAAACCC GCUCAAUGCC UGGAGAUUUG GGCGUGCCCC CGCGAGACUG CUAGCCGAGU AGUGUUGGGU CGCGAAAGGC CUUGUGGUAC UGCCUGAUAG GGUGCUUGCG AGUGCCCCGG GAGGUCUCGU

• Mass: Theoretical: 93.491078 kDa
• Source: Species: Human (human)

Experimental details

Sample preparation

• Specimen: Specimen state: Particle / Method: cryo EM
• Sample solution: pH: 7.5
• Vitrification: Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 277 K / Humidity: 100 %

Electron microscopy imaging

• Experimental equipment: Model: Talos Arctica / Image courtesy: FEI Company
• Imaging: Microscope: FEI TECNAI ARCTICA
• Electron gun: Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 50 e/Ų / Illumination mode: FLOOD BEAM
• Lens: Magnification: 23500.0 X (nominal), 33557.0 X (calibrated) / Cs: 2.7 mm / Imaging mode: BRIGHT FIELD
• Specimen Holder: Model: OTHER
• Camera: Detector: GATAN K2 SUMMIT (4k x 4k)

Image processing

• Processing: Method: single particle reconstruction / Applied symmetry: C₁ (asymmetric) / Number of projections: 48923
• 3D reconstruction: Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF

Atomic model buiding

Output model

6ip8