+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-11362 | |||||||||
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タイトル | Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Dis-B (Poly-Ala) | |||||||||
マップデータ | ||||||||||
試料 |
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キーワード | ribosome / 90S pre-ribosome / 40S pre-ribosome / A1 cleavage / Dhr1 | |||||||||
機能・相同性 | 機能・相同性情報 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / Noc4p-Nop14p complex / regulation of ribosomal protein gene transcription by RNA polymerase II / rRNA small subunit pseudouridine methyltransferase Nep1 / CURI complex / UTP-C complex / Pwp2p-containing subcomplex of 90S preribosome / nuclear microtubule / Mpp10 complex ...18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / Noc4p-Nop14p complex / regulation of ribosomal protein gene transcription by RNA polymerase II / rRNA small subunit pseudouridine methyltransferase Nep1 / CURI complex / UTP-C complex / Pwp2p-containing subcomplex of 90S preribosome / nuclear microtubule / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / rRNA modification / regulation of rRNA processing / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA (adenine-N6,N6-)-dimethyltransferase activity / septum digestion after cytokinesis / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / single-stranded telomeric DNA binding / tRNA export from nucleus / rRNA base methylation / Negative regulators of DDX58/IFIH1 signaling / rRNA primary transcript binding / sno(s)RNA-containing ribonucleoprotein complex / mTORC1-mediated signalling / Protein hydroxylation / rRNA methylation / U3 snoRNA binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / poly(A)+ mRNA export from nucleus / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / preribosome, small subunit precursor / Ribosomal scanning and start codon recognition / snoRNA binding / establishment of cell polarity / Major pathway of rRNA processing in the nucleolus and cytosol / nucleolar large rRNA transcription by RNA polymerase I / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / proteasome assembly / 90S preribosome / regulation of translational fidelity / Ub-specific processing proteases / ribosomal subunit export from nucleus / ribosomal small subunit export from nucleus / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / enzyme activator activity / RNA endonuclease activity / ribosome assembly / nuclear periphery / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / translational initiation / helicase activity / maintenance of translational fidelity / rRNA processing / ribosomal small subunit biogenesis / unfolded protein binding / small ribosomal subunit rRNA binding / ribosome biogenesis / ribosomal small subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / cytoplasmic translation / RNA helicase activity / rRNA binding / RNA helicase / ribosome / structural constituent of ribosome / translation / GTPase activity / mRNA binding / GTP binding / nucleolus / ATP hydrolysis activity / mitochondrion / RNA binding / nucleoplasm / ATP binding / identical protein binding / nucleus / metal ion binding / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | accharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) / Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) / Saccharomyces cerevisiae S288C (パン酵母) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.9 Å | |||||||||
データ登録者 | Cheng J / Lau B | |||||||||
引用 | ジャーナル: Science / 年: 2020 タイトル: 90 pre-ribosome transformation into the primordial 40 subunit. 著者: Jingdong Cheng / Benjamin Lau / Giuseppe La Venuta / Michael Ameismeier / Otto Berninghausen / Ed Hurt / Roland Beckmann / 要旨: Production of small ribosomal subunits initially requires the formation of a 90 precursor followed by an enigmatic process of restructuring into the primordial pre-40 subunit. We elucidate this ...Production of small ribosomal subunits initially requires the formation of a 90 precursor followed by an enigmatic process of restructuring into the primordial pre-40 subunit. We elucidate this process by biochemical and cryo-electron microscopy analysis of intermediates along this pathway in yeast. First, the remodeling RNA helicase Dhr1 engages the 90 pre-ribosome, followed by Utp24 endonuclease-driven RNA cleavage at site A, thereby separating the 5'-external transcribed spacer (ETS) from 18 ribosomal RNA. Next, the 5'-ETS and 90 assembly factors become dislodged, but this occurs sequentially, not en bloc. Eventually, the primordial pre-40 emerges, still retaining some 90 factors including Dhr1, now ready to unwind the final small nucleolar U3-18 RNA hybrid. Our data shed light on the elusive 90 to pre-40 transition and clarify the principles of assembly and remodeling of large ribonucleoproteins. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_11362.map.gz | 31.3 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-11362-v30.xml emd-11362.xml | 63.3 KB 63.3 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_11362_fsc.xml | 17.1 KB | 表示 | FSCデータファイル |
画像 | emd_11362.png | 110 KB | ||
Filedesc metadata | emd-11362.cif.gz | 17.6 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-11362 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-11362 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_11362_validation.pdf.gz | 419.3 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_11362_full_validation.pdf.gz | 418.8 KB | 表示 | |
XML形式データ | emd_11362_validation.xml.gz | 15.3 KB | 表示 | |
CIF形式データ | emd_11362_validation.cif.gz | 21.1 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-11362 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-11362 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_11362.map.gz / 形式: CCP4 / 大きさ: 421.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.059 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
+全体 : 90S pre-ribbosome state Dis-B
+超分子 #1: 90S pre-ribbosome state Dis-B
+分子 #1: Periodic tryptophan protein 2
+分子 #2: Nucleolar complex protein 14
+分子 #3: Something about silencing protein 10
+分子 #4: U3 small nucleolar RNA-associated protein 12
+分子 #5: U3 small nucleolar RNA-associated protein 13
+分子 #6: Nucleolar complex protein 4
+分子 #7: U3 small nucleolar RNA-associated protein 21
+分子 #8: U3 small nucleolar RNA-associated protein 22
+分子 #9: U3 small nucleolar ribonucleoprotein protein IMP3
+分子 #10: U3 small nucleolar ribonucleoprotein protein IMP4
+分子 #11: U3 small nucleolar RNA-associated protein MPP10
+分子 #12: Ribosome biogenesis protein BMS1
+分子 #13: RNA 3'-terminal phosphate cyclase-like protein
+分子 #14: Ribosomal RNA-processing protein 7
+分子 #15: Probable ATP-dependent RNA helicase DHR1
+分子 #16: Ribosomal RNA small subunit methyltransferase NEP1
+分子 #17: Essential nuclear protein 1
+分子 #18: Dimethyladenosine transferase
+分子 #19: Pre-rRNA-processing protein PNO1
+分子 #20: Rps5p
+分子 #21: 40S ribosomal protein S16-A
+分子 #22: 40S ribosomal protein S18-A
+分子 #23: 40S ribosomal protein S19-A
+分子 #24: 40S ribosomal protein S28-A
+分子 #27: 40S ribosomal protein S1-A
+分子 #28: 40S ribosomal protein S4-A
+分子 #29: 40S ribosomal protein S6-A
+分子 #30: 40S ribosomal protein S7-A
+分子 #31: 40S ribosomal protein S8-A
+分子 #32: 40S ribosomal protein S9-A
+分子 #33: 40S ribosomal protein S11-A
+分子 #34: 40S ribosomal protein S13
+分子 #35: 40S ribosomal protein S14-A
+分子 #36: 40S ribosomal protein S25-A
+分子 #37: 40S ribosomal protein S22-A
+分子 #38: 40S ribosomal protein S23-A
+分子 #39: 40S ribosomal protein S24-A
+分子 #40: 40S ribosomal protein S27-A
+分子 #25: 5ETS RNA
+分子 #26: 18S rRNA
+分子 #41: U3 snoRNA
+分子 #42: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.4 |
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グリッド | モデル: Quantifoil / 材質: COPPER / 支持フィルム - 材質: CARBON |
凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 44.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |