登録情報 データベース : EMDB / ID : EMD-10412 構造の表示 ダウンロードとリンクタイトル cryo-EM structure of transcription coactivator SAGA マップデータ 詳細 試料 詳細 キーワード Coactivator / Transcription / Histone acetyltransferase / Histone deubiquitinase / GENE REGULATION機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
RITS complex assembly / conjugation with cellular fusion / DUBm complex / pseudohyphal growth / positive regulation of DNA-templated transcription initiation / invasive growth in response to glucose limitation / TTT Hsp90 cochaperone complex / regulation of nucleocytoplasmic transport / SLIK (SAGA-like) complex / regulation of protein localization to chromatin ... RITS complex assembly / conjugation with cellular fusion / DUBm complex / pseudohyphal growth / positive regulation of DNA-templated transcription initiation / invasive growth in response to glucose limitation / TTT Hsp90 cochaperone complex / regulation of nucleocytoplasmic transport / SLIK (SAGA-like) complex / regulation of protein localization to chromatin / SAGA complex / RNA polymerase II transcribes snRNA genes / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / IRE1-mediated unfolded protein response / RNA Polymerase II Pre-transcription Events / DNA repair-dependent chromatin remodeling / NuA4 histone acetyltransferase complex / positive regulation of RNA polymerase II transcription preinitiation complex assembly / Ub-specific processing proteases / mRNA export from nucleus / RNA polymerase II preinitiation complex assembly / TBP-class protein binding / ubiquitin binding / transcription coregulator activity / transcription initiation at RNA polymerase II promoter / enzyme activator activity / promoter-specific chromatin binding / peroxisome / chromatin organization / protein-containing complex assembly / molecular adaptor activity / RNA polymerase II-specific DNA-binding transcription factor binding / transcription by RNA polymerase II / transcription coactivator activity / chromatin remodeling / protein heterodimerization activity / DNA repair / DNA-templated transcription / chromatin binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / structural molecule activity / positive regulation of transcription by RNA polymerase II / mitochondrion / DNA binding / identical protein binding / nucleus / cytosol 類似検索 - 分子機能 Transcriptional activator Spt7 / Transcriptional coactivator Hfi1/Transcriptional adapter 1 / Transcriptional regulator of RNA polII, SAGA, subunit / Transcription factor Spt20 / Spt20-like, SEP domain / Spt20, SEP domain / SAGA-associated factor 73, zinc finger domain / Zinc finger domain / SAGA-associated factor 73 / LisH ... Transcriptional activator Spt7 / Transcriptional coactivator Hfi1/Transcriptional adapter 1 / Transcriptional regulator of RNA polII, SAGA, subunit / Transcription factor Spt20 / Spt20-like, SEP domain / Spt20, SEP domain / SAGA-associated factor 73, zinc finger domain / Zinc finger domain / SAGA-associated factor 73 / LisH / SCA7 domain / SCA7, zinc-binding domain / SCA7 domain profile. / Tra1, HEAT repeat ring region / Tra1, HEAT repeat central region / Tra1 HEAT repeat central region / Tra1 HEAT repeat ring region / Transcription initiation factor IID, subunit 13 / Transcription initiation factor IID, 18kD subunit / : / Transcription initiation factor IID, 31kD subunit / Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID subunit A / TFIID subunit TAF5, NTD2 domain superfamily / WD40 associated region in TFIID subunit, NTD2 domain / Transcription initiation factor TAFII31 / TATA box binding protein associated factor (TAF) / Transcription initiation factor TFIID subunit 12 domain / TFIID subunit TAF5, NTD2 domain / Transcription initiation factor TFIID subunit 6 / TAF6, C-terminal HEAT repeat domain superfamily / TAF6 C-terminal HEAT repeat domain / TAF6, C-terminal HEAT repeat domain / Transcription initiation factor TFIID 23-30kDa subunit / Transcription initiation factor TFIID, 23-30kDa subunit / Lissencephaly type-1-like homology motif / LIS1 homology (LisH) motif profile. / LIS1 homology motif / : / PIK-related kinase, FAT / FAT domain / FATC domain / PIK-related kinase / FAT domain profile. / FATC domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / Phosphoinositide 3-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF), histone-like fold domain / Bromodomain, conserved site / Bromodomain signature. / Histone-fold / Bromodomain / bromo domain / Bromodomain (BrD) profile. / Bromodomain / Bromodomain-like superfamily / WD domain, G-beta repeat / Armadillo-type fold / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Protein kinase-like domain superfamily 類似検索 - ドメイン・相同性 Protein SPT3 / Transcriptional activator SPT7 / Transcription initiation factor TFIID subunit 5 / Transcription-associated protein 1 / SAGA complex subunit SPT20 / Transcription initiation factor TFIID subunit 6 / SAGA complex subunit SGF73 / SAGA complex/transcription factor TFIID complex subunit TAF12 / Transcription initiation factor TFIID subunit 9 / Transcription initiation factor TFIID subunit 10 / Transcriptional coactivator HFI1/ADA1 類似検索 - 構成要素生物種 Saccharomyces cerevisiae S288C (パン酵母) / Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.9 Å 詳細 データ登録者Wang H / Cheung A 資金援助 ドイツ, 2件 詳細 詳細を隠すOrganization Grant number 国 German Research Foundation SFB860, SPP1935, EXC 2067/1-390729940 ドイツ European Research Council 693023 ドイツ
引用ジャーナル : Nature / 年 : 2020タイトル : Structure of the transcription coactivator SAGA.著者 : Haibo Wang / Christian Dienemann / Alexandra Stützer / Henning Urlaub / Alan C M Cheung / Patrick Cramer / 要旨 : Gene transcription by RNA polymerase II is regulated by activator proteins that recruit the coactivator complexes SAGA (Spt-Ada-Gcn5-acetyltransferase) and transcription factor IID (TFIID). SAGA is ... Gene transcription by RNA polymerase II is regulated by activator proteins that recruit the coactivator complexes SAGA (Spt-Ada-Gcn5-acetyltransferase) and transcription factor IID (TFIID). SAGA is required for all regulated transcription and is conserved among eukaryotes. SAGA contains four modules: the activator-binding Tra1 module, the core module, the histone acetyltransferase (HAT) module and the histone deubiquitination (DUB) module. Previous studies provided partial structures, but the structure of the central core module is unknown. Here we present the cryo-electron microscopy structure of SAGA from the yeast Saccharomyces cerevisiae and resolve the core module at 3.3 Å resolution. The core module consists of subunits Taf5, Sgf73 and Spt20, and a histone octamer-like fold. The octamer-like fold comprises the heterodimers Taf6-Taf9, Taf10-Spt7 and Taf12-Ada1, and two histone-fold domains in Spt3. Spt3 and the adjacent subunit Spt8 interact with the TATA box-binding protein (TBP). The octamer-like fold and its TBP-interacting region are similar in TFIID, whereas Taf5 and the Taf6 HEAT domain adopt distinct conformations. Taf12 and Spt20 form flexible connections to the Tra1 module, whereas Sgf73 tethers the DUB module. Binding of a nucleosome to SAGA displaces the HAT and DUB modules from the core-module surface, allowing the DUB module to bind one face of an ubiquitinated nucleosome. 履歴 登録 2019年10月28日 - ヘッダ(付随情報) 公開 2019年11月27日 - マップ公開 2020年1月29日 - 更新 2024年5月22日 - 現状 2024年5月22日 処理サイト : PDBe / 状態 : 公開
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