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- EMDB-4093: Entire ovine respiratory complex I, Combined Map -

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Basic information

Entry
Database: EMDB / ID: EMD-4093
TitleEntire ovine respiratory complex I, Combined Map
Map data
Sample
  • Complex: Mitochondrial complex I
    • Protein or peptide: x 44 types
  • Ligand: x 10 types
KeywordsNADH:ubiquinone / oxidoreductase / complex I / mammalian / mitochondrial
Function / homology
Function and homology information


Complex I biogenesis / : / : / Respiratory electron transport / oxidoreductase activity, acting on NAD(P)H / mitochondrial ATP synthesis coupled electron transport / apoptotic mitochondrial changes / Mitochondrial protein degradation / acyl binding / ubiquinone binding ...Complex I biogenesis / : / : / Respiratory electron transport / oxidoreductase activity, acting on NAD(P)H / mitochondrial ATP synthesis coupled electron transport / apoptotic mitochondrial changes / Mitochondrial protein degradation / acyl binding / ubiquinone binding / acyl carrier activity / electron transport coupled proton transport / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / NADH dehydrogenase activity / respiratory chain complex I / NADH dehydrogenase (ubiquinone) activity / membrane => GO:0016020 / quinone binding / ATP metabolic process / ATP synthesis coupled electron transport / reactive oxygen species metabolic process / aerobic respiration / regulation of mitochondrial membrane potential / respiratory electron transport chain / electron transport chain / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / circadian rhythm / NAD binding / FMN binding / 4 iron, 4 sulfur cluster binding / response to oxidative stress / mitochondrial inner membrane / mitochondrial matrix / negative regulation of DNA-templated transcription / apoptotic process / protein-containing complex binding / mitochondrion / nucleoplasm / metal ion binding / cytoplasm
Similarity search - Function
NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 ...NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, animal type / NADH dehydrogenase 1 beta subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / : / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 alpha subcomplex subunit 3 / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / NDUFA6, LYR domain / Deoxynucleoside kinase domain / Deoxynucleoside kinase / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / : / SLBB domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / : / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH-quinone oxidoreductase, chain M/4 / NADH-ubiquinone oxidoreductase chain 4L/K / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NAD(P)H-quinone oxidoreductase subunit D/H / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / Complex 1 LYR protein domain / NADH-quinone oxidoreductase, subunit D / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH ubiquinone oxidoreductase, F subunit / Complex 1 protein (LYR family) / NADH dehydrogenase, subunit C / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 30 Kd subunit signature. / NADH:ubiquinone oxidoreductase
Similarity search - Domain/homology
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial ...NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / Acyl carrier protein / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 subunit C2 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
Similarity search - Component
Biological speciesOvis aries (sheep)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsFiedorczuk K / Letts JA / Sazanov LA
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Medical Research Council (United Kingdom)MC_U105674180 United Kingdom
CitationJournal: Nature / Year: 2016
Title: Atomic structure of the entire mammalian mitochondrial complex I.
Authors: Karol Fiedorczuk / James A Letts / Gianluca Degliesposti / Karol Kaszuba / Mark Skehel / Leonid A Sazanov /
Abstract: Mitochondrial complex I (also known as NADH:ubiquinone oxidoreductase) contributes to cellular energy production by transferring electrons from NADH to ubiquinone coupled to proton translocation ...Mitochondrial complex I (also known as NADH:ubiquinone oxidoreductase) contributes to cellular energy production by transferring electrons from NADH to ubiquinone coupled to proton translocation across the membrane. It is the largest protein assembly of the respiratory chain with a total mass of 970 kilodaltons. Here we present a nearly complete atomic structure of ovine (Ovis aries) mitochondrial complex I at 3.9 Å resolution, solved by cryo-electron microscopy with cross-linking and mass-spectrometry mapping experiments. All 14 conserved core subunits and 31 mitochondria-specific supernumerary subunits are resolved within the L-shaped molecule. The hydrophilic matrix arm comprises flavin mononucleotide and 8 iron-sulfur clusters involved in electron transfer, and the membrane arm contains 78 transmembrane helices, mostly contributed by antiporter-like subunits involved in proton translocation. Supernumerary subunits form an interlinked, stabilizing shell around the conserved core. Tightly bound lipids (including cardiolipins) further stabilize interactions between the hydrophobic subunits. Subunits with possible regulatory roles contain additional cofactors, NADPH and two phosphopantetheine molecules, which are shown to be involved in inter-subunit interactions. We observe two different conformations of the complex, which may be related to the conformationally driven coupling mechanism and to the active-deactive transition of the enzyme. Our structure provides insight into the mechanism, assembly, maturation and dysfunction of mitochondrial complex I, and allows detailed molecular analysis of disease-causing mutations.
History
DepositionOct 28, 2016-
Header (metadata) releaseNov 23, 2016-
Map releaseNov 23, 2016-
UpdateApr 9, 2025-
Current statusApr 9, 2025Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.15
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.15
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5lnk
  • Surface level: 0.15
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4093.png

Downloads & links

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Map

FileDownload / File: emd_4093.map.gz / Format: CCP4 / Size: 14.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
1.39 Å/pix.
x 129 pix.
= 179.31 Å		1.39 Å/pix.
x 141 pix.
= 195.99 Å		1.39 Å/pix.
x 209 pix.
= 290.51 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 1.39 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.15 / Movie #1: 0.15
Minimum - Maximum-0.5494037 - 1.3139707
Average (Standard dev.)0.0089633055 (±0.05093917)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions141209129
Spacing129141209
CellA: 179.31 Å / B: 195.99 Å / C: 290.51 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.391.391.39
M x/y/z129141209
origin x/y/z0.0000.0000.000
length x/y/z179.310195.990290.510
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ129141209
MAP C/R/S321
start NC/NR/NS000
NC/NR/NS209141129
D min/max/mean-0.5491.3140.009

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Supplemental data

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Sample components

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Entire : Mitochondrial complex I

EntireName: Mitochondrial complex I
Components
  • Complex: Mitochondrial complex I
    • Protein or peptide: Mitochondrial complex I, 51 kDa subunit
    • Protein or peptide: Mitochondrial complex I, 24 kDa subunit
    • Protein or peptide: Mitochondrial complex I, 75 kDa subunit
    • Protein or peptide: Mitochondrial complex I, 49 kDa subunit
    • Protein or peptide: Mitochondrial complex I, 30 kDa subunit
    • Protein or peptide: Mitochondrial complex I, PSST subunit
    • Protein or peptide: Mitochondrial complex I, TYKY subunit
    • Protein or peptide: Mitochondrial complex I, ND1 subunit
    • Protein or peptide: Mitochondrial complex I, ND2 subunit
    • Protein or peptide: Mitochondrial complex I, ND3 subunit
    • Protein or peptide: Mitochondrial complex I, ND4 subunit
    • Protein or peptide: Mitochondrial complex I, ND4L subunit
    • Protein or peptide: Mitochondrial complex I, ND5 subunit
    • Protein or peptide: Mitochondrial complex I, ND6 subunit
    • Protein or peptide: Mitochondrial complex I, 10 kDa subunit
    • Protein or peptide: Mitochondrial complex I, 13 kDa subunit
    • Protein or peptide: Mitochondrial complex I, 18 kDa subunit
    • Protein or peptide: Mitochondrial complex I, 39 kDa subunit
    • Protein or peptide: Mitochondrial complex I, B8 subunit
    • Protein or peptide: Mitochondrial complex I, B13 subunit
    • Protein or peptide: Mitochondrial complex I, B14 subunit
    • Protein or peptide: Mitochondrial complex I, B14.5a subunit
    • Protein or peptide: Mitochondrial complex I, B17.2 subunit
    • Protein or peptide: Acyl carrier protein
    • Protein or peptide: Mitochondrial complex I, 42 kDa subunit
    • Protein or peptide: Mitochondrial complex I, 15 kDa subunit
    • Protein or peptide: Mitochondrial complex I, B9 subunit
    • Protein or peptide: Mitochondrial complex I, B12 subunit
    • Protein or peptide: Mitochondrial complex I, B14.5b subunit
    • Protein or peptide: Mitochondrial complex I, B15 subunit
    • Protein or peptide: Mitochondrial complex I, B16.6 subunit
    • Protein or peptide: Mitochondrial complex I, B17 subunit
    • Protein or peptide: Mitochondrial complex I, B18 subunit
    • Protein or peptide: Mitochondrial complex I, B22 subunit
    • Protein or peptide: Mitochondrial complex I, AGGG subunit
    • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
    • Protein or peptide: Mitochondrial complex I, ESSS subunit
    • Protein or peptide: Mitochondrial complex I, KFYI subunit
    • Protein or peptide: Mitochondrial complex I, MNLL subunit
    • Protein or peptide: Mitochondrial complex I, MWFE subunit
    • Protein or peptide: Mitochondrial complex I, PDSW subunit
    • Protein or peptide: Mitochondrial complex I, PGIV subunit
    • Protein or peptide: Mitochondrial complex I, SGDH subunit
    • Protein or peptide: Mitochondrial complex I, B14.7 subunit
  • Ligand: IRON/SULFUR CLUSTER
  • Ligand: FLAVIN MONONUCLEOTIDE
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
  • Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
  • Ligand: CARDIOLIPIN
  • Ligand: ZINC ION
  • Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
  • Ligand: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
  • Ligand: 4'-PHOSPHOPANTETHEINE

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Supramolecule #1: Mitochondrial complex I

SupramoleculeName: Mitochondrial complex I / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 970 KDa

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Macromolecule #1: Mitochondrial complex I, 51 kDa subunit

MacromoleculeName: Mitochondrial complex I, 51 kDa subunit / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 48.678281 KDa
SequenceString: FSGDTTAPKK TSFGSLKDED RIFTNLYGRH DWRLKGAQSR GDWYKTKEIL LKGPDWILGE VKTSGLRGRG GAGFPTGLKW SFMNKPSDG RPKYLVVNAD EGEPGTCKDR EIIRHDPHKL VEGCLVGGRA MGARAAYIYI RGEFYNEASN LQVAIREAYE A GLIGKNAC ...String:
FSGDTTAPKK TSFGSLKDED RIFTNLYGRH DWRLKGAQSR GDWYKTKEIL LKGPDWILGE VKTSGLRGRG GAGFPTGLKW SFMNKPSDG RPKYLVVNAD EGEPGTCKDR EIIRHDPHKL VEGCLVGGRA MGARAAYIYI RGEFYNEASN LQVAIREAYE A GLIGKNAC GSGYDFDVFV VRGAGAYICG EETALIESIE GKQGKPRLKP PFPADVGVFG CPTTVANVET VAVSPTICRR GG AWFASFG RERNSGTKLF NISGHVNNPC TVEEEMSVPL KELIEKHAGG VTGGWDNLLA VIPGGSSTPL IPKSVCETVL MDF DALIQA QTGLGTAAVI VMDRSTDIVK AIARLIEFYK HESCGQCTPC REGVDWMNKV MARFVRGDAR PAEIDSLWEI SKQI EGHTI CALGDGAAWP VQGLIRHFRP ELEERMQRFA QQHQARQAAS

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

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Macromolecule #2: Mitochondrial complex I, 24 kDa subunit

MacromoleculeName: Mitochondrial complex I, 24 kDa subunit / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 23.840428 KDa
SequenceString: GAGGALFVHR DTPKNNPETP FDFTPENYKR IEAIVKNYPE GHKAAAVLPV LDLAQRQNGW LPISAMNKVA EILQVPPMRV YEVATFYTM YNRKPVGKYH IQVCTTTPCM LRNSDSILEA IQKKLGIKVG ETTPDKLFTL IEVECLGACV NAPMVQINDN Y YEDLTPKD ...String:
GAGGALFVHR DTPKNNPETP FDFTPENYKR IEAIVKNYPE GHKAAAVLPV LDLAQRQNGW LPISAMNKVA EILQVPPMRV YEVATFYTM YNRKPVGKYH IQVCTTTPCM LRNSDSILEA IQKKLGIKVG ETTPDKLFTL IEVECLGACV NAPMVQINDN Y YEDLTPKD IEEIIDELKA GKIPKPGPRS GRFSCEPAGG LTSLTEPPKG PGFGVQAGL

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

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Macromolecule #3: Mitochondrial complex I, 75 kDa subunit

MacromoleculeName: Mitochondrial complex I, 75 kDa subunit / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 77.031156 KDa
SequenceString: TATAASNLIE VFVDGQSVMV EPGTTVLQAC EKVGMQIPRF CYHERLSVAG NCRMCLVEIE KAPKVVAACA MPVMKGWNIL TNSEKTKKA REGVMEFLLA NHPLDCPICD QGGECDLQDQ SMMFGSDRSR FLEGKRAVED KNIGPLVKTI MTRCIQCTRC I RFASEIAG ...String:
TATAASNLIE VFVDGQSVMV EPGTTVLQAC EKVGMQIPRF CYHERLSVAG NCRMCLVEIE KAPKVVAACA MPVMKGWNIL TNSEKTKKA REGVMEFLLA NHPLDCPICD QGGECDLQDQ SMMFGSDRSR FLEGKRAVED KNIGPLVKTI MTRCIQCTRC I RFASEIAG VDDLGTTGRG NDMQVGTYIE KMFMSELSGN IIDICPVGAL TSKPYAFTAR PWETRKTESI DVMDAVGSNI VV STRTGEV MRILPRMHED INEEWISDKT RFAYDGLKRQ RLTEPMVRNE KGLLTHTTWE DALSRVAGML QSCQGNDVAA IAG GLVDAE ALIALKDLLN RVDSDTLCTE EVFPTAGAGT DLRSNYLLNT TIAGVEEADV VLLVGTNPRF EAPLFNARIR KSWL HNDLK VALIGSPVDL TYRYDHLGDS PKILQDIASG SHPFSQVLQE AKKPMVVLGS SALQRNDGAA ILAAVSNIAQ KIRTS SGVT GDWKVMNILH RIASQVAALD LGYKPGVEAI RKNPPKMLFL LGADGGCVTR QDLPKDCFIV YQGHHGDVGA PIADVI LPG AAYTEKSATY VNTEGRAQQT KVAVMPPGLA REDWKIIRAL SEIAGMTLPY DTLDQVRNRL EEVSPNLVRY DDVEGAN YF QQASELSKLV NQQLLADPLV PPQLTIKDFY MTDSISRASQ TMAKCVKAVT EGAHAVEEPS IC

UniProtKB: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

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Macromolecule #4: Mitochondrial complex I, 49 kDa subunit

MacromoleculeName: Mitochondrial complex I, 49 kDa subunit / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 47.120078 KDa
SequenceString: MYPTKETAHW KPPPWNDVDP PKDTLVSNLT LNFGPQHPAA HGVLRLVMEL SGEMVRKCDP HIGLLHRGTE KLIEYKTYLQ ALPYFDRLD YVSMMCNEQA YSLAVEKLLN IQPPPRAQWI RVLFGEITRL LNHIMAVTTH ALDIGAMTPF FWMFEEREKM F EFYERVSG ...String:
MYPTKETAHW KPPPWNDVDP PKDTLVSNLT LNFGPQHPAA HGVLRLVMEL SGEMVRKCDP HIGLLHRGTE KLIEYKTYLQ ALPYFDRLD YVSMMCNEQA YSLAVEKLLN IQPPPRAQWI RVLFGEITRL LNHIMAVTTH ALDIGAMTPF FWMFEEREKM F EFYERVSG ARMHAAYVRP GGVHQDLPLG LMDDIYEFSK NFSLRIDELE EMLTNNRIWR NRTVDIGVVT AEDALNYGFS GV MLRGSGI QWDLRKTQPY DVYDQVEFDV PIGSRGDCYD RYLCRVEEMR QSIRIISQCL NKMPPGEIKV DDAKVSPPKR AEM KTSMES LIHHFKLYTE GYQVPPGATY TAIEAPKGEF GVYLVSDGSS RPYRCKIKAP GFAHLAGLDK MSKGHMLADV VAII GTQDI VFGEVDR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

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Macromolecule #5: Mitochondrial complex I, 30 kDa subunit

MacromoleculeName: Mitochondrial complex I, 30 kDa subunit / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 26.441793 KDa
SequenceString: ESASADTRPT VRPRNDVAHK QLSAFGEYVA EILPKYVQQV QVSCFSELEI CIHPDGVIPV LTFLRDHSNA QFKSLADLTA VDIPTRQNR FEIVYNLLSL RFNSRIRVKT YTDELTPVES SVSVYKAANW YEREIWDMFG VFFANHPDLR RILTDYGFEG H PFRKDFPL ...String:
ESASADTRPT VRPRNDVAHK QLSAFGEYVA EILPKYVQQV QVSCFSELEI CIHPDGVIPV LTFLRDHSNA QFKSLADLTA VDIPTRQNR FEIVYNLLSL RFNSRIRVKT YTDELTPVES SVSVYKAANW YEREIWDMFG VFFANHPDLR RILTDYGFEG H PFRKDFPL SGYVELRYDD EVKRVVAEPV ELAQEFRKFD LNSPWEAFPA YRQPPESLKL EAGDKKPEAK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

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Macromolecule #6: Mitochondrial complex I, PSST subunit

MacromoleculeName: Mitochondrial complex I, PSST subunit / type: protein_or_peptide / ID: 6 / Details: NCBIProt XP_012033846.2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 20.104531 KDa
SequenceString:
PSSTQPAVSQ ARAVVPKPAA LPSSRGEYVV AKLDDLINWA RRSSLWPMTF GLACCAVEMM HMAAPRYDMD RFGVVFRASP RQSDVMIVA GTLTNKMAPA LRKVYDQMPE PRYVVSMGSC ANGGGYYHYS YSVVRGCDRI VPVDIYVPGC PPTAEALLYG I LQLQKKIK REKRLRIWYR R

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Macromolecule #7: Mitochondrial complex I, TYKY subunit

MacromoleculeName: Mitochondrial complex I, TYKY subunit / type: protein_or_peptide / ID: 7 / Details: NCBIprot XP_011972879.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 20.219947 KDa
SequenceString:
TYKYVNLREP SMDMKSVTDR AAQTLLWTEL IRGLGMTLSY LFREPATINY PFEKGPLSPR FRGEHALRRY PSGEERCIAC KLCEAVCPA QAITIEAEPR ADGSRRTTRY DIDMTKCIYC GFCQEACPVD AIVEGPNFEF STETHEELLY NKEKLLNNGD K WEAEIAAN IQADYLYR

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Macromolecule #8: Mitochondrial complex I, ND1 subunit

MacromoleculeName: Mitochondrial complex I, ND1 subunit / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 35.884902 KDa
SequenceString: MFMINVLTLI IPILLAVAFL TLVERKVLGY MQFRKGPNVV GPYGLLQPIA DAIKLFIKEP LRPATSSISM FILAPILALT LALTMWIPL PMPYPLINMN LGVLFMLAMS SLAVYSILWS GWASNSKYAL IGALRAVAQT ISYEVTLAII LLSVLLMNGS F TLSTLIIT ...String:
MFMINVLTLI IPILLAVAFL TLVERKVLGY MQFRKGPNVV GPYGLLQPIA DAIKLFIKEP LRPATSSISM FILAPILALT LALTMWIPL PMPYPLINMN LGVLFMLAMS SLAVYSILWS GWASNSKYAL IGALRAVAQT ISYEVTLAII LLSVLLMNGS F TLSTLIIT QEQVWLIFPA WPLAMMWFIS TLAETNRAPF DLTEGESELV SGFNVEYAAG PFALFFMAEY ANIIMMNIFT TT LFLGAFH NPYMPELYTI NFTIKSLLLS ITFLWIRASY PRFRYDQLMH LLWKNFLPLT LALCMWHVSL PILLSSIPPQ T

UniProtKB: NADH-ubiquinone oxidoreductase chain 1

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Macromolecule #9: Mitochondrial complex I, ND2 subunit

MacromoleculeName: Mitochondrial complex I, ND2 subunit / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 39.149805 KDa
SequenceString: MNPIILIIIL MTVMLGTIIV MISTHWLLIW IGFEMNMLAI IPIMMKKHNP RATEASTKYF LTQSTASMLL MMAIIINLMF SGQWTVMKL FNPMASMLMT MALAMKLGMA PFHFWVPEVT QGIPLSSGLI LLTWQKLAPM SVLYQILPSI NLDLILTLSI L SITIGGWG ...String:
MNPIILIIIL MTVMLGTIIV MISTHWLLIW IGFEMNMLAI IPIMMKKHNP RATEASTKYF LTQSTASMLL MMAIIINLMF SGQWTVMKL FNPMASMLMT MALAMKLGMA PFHFWVPEVT QGIPLSSGLI LLTWQKLAPM SVLYQILPSI NLDLILTLSI L SITIGGWG GLNQTQLRKI MAYSSIAHMG WMTAVLLYNP TMTLLNLIIY IIMTSTMFTL FMANSTTTTL SLSHTWNKAP IM TILVLIT LLSMGGLPPL SGFMPKWMII QEMTKNDSII LPTLMAITAL LNLYFYMRLT YSTALTMFPS TNNMKMKWQF PTT KRMTLL PTMTVLSTML LPLTPILSIL E

UniProtKB: NADH-ubiquinone oxidoreductase chain 2

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Macromolecule #10: Mitochondrial complex I, ND3 subunit

MacromoleculeName: Mitochondrial complex I, ND3 subunit / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 13.10652 KDa
SequenceString:
MNLMITLLTN FTLATLLVTI AFWLPQLNVY SEKTSPYECG FDPMGSARLP FSMKFFLVAI TFLLFDLEIA LLLPLPWASQ TTNLNTMLT MALLLIFLLA VSLAYEWTQK GLEWTE

UniProtKB: NADH-ubiquinone oxidoreductase chain 3

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Macromolecule #11: Mitochondrial complex I, ND4 subunit

MacromoleculeName: Mitochondrial complex I, ND4 subunit / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 52.058734 KDa
SequenceString: MLKYIIPTMM LMPLTWLSKN SMIWINTTLH SLLISLTSLL LLNQFGDNSL NFSLTFFSDS LSTPLLILTM WLLPLMLMAS QHHLSKENL ARKKLFISML ILLQLFLIMT FTATELIFFY IMFEATLVPT LIIITRWGNQ TERLNAGLYF LFYTLAGSLP L LVALIYIQ ...String:
MLKYIIPTMM LMPLTWLSKN SMIWINTTLH SLLISLTSLL LLNQFGDNSL NFSLTFFSDS LSTPLLILTM WLLPLMLMAS QHHLSKENL ARKKLFISML ILLQLFLIMT FTATELIFFY IMFEATLVPT LIIITRWGNQ TERLNAGLYF LFYTLAGSLP L LVALIYIQ NTMGSLNFLI LQYWVQPMPN SWSNTFMWLA CMMAFMVKMP LYGLHLWLPK AHVEAPIAGS MVLAAILLKL GG YGMMRIT LLLNPITDFM AYPFIMLSLW GMIMTSSICL RQTDLKSLIA YSSVSHMALV IVAILIQTPW SYMGATALMI AHG LTSSML FCLANSNYER VHSRTMILAR GLQTLLPLMA AWWLLASLTN LALPPSINLI GELFVVMSTF SWSNITIILM GLNM VITAL YSLYMLITTQ RGKHTHHINN ILPSFTRENA LMSLHMLPLL LLSLNPKIIL GPLY

UniProtKB: NADH-ubiquinone oxidoreductase chain 4

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Macromolecule #12: Mitochondrial complex I, ND4L subunit

MacromoleculeName: Mitochondrial complex I, ND4L subunit / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 10.840228 KDa
SequenceString:
MSLVYMNIMM AFTVSLTGLL MYRSHLMSSL LCLEGMMLSL FILATLMILN SHFTLASMMP IILLVFAACE AALGLSLLVM VSNTYGTDY VQNLNLLQC

UniProtKB: NADH-ubiquinone oxidoreductase chain 4L

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Macromolecule #13: Mitochondrial complex I, ND5 subunit

MacromoleculeName: Mitochondrial complex I, ND5 subunit / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 67.554945 KDa
SequenceString: MNLFSSLTLV TLILLTMPIA AINFNTHKFT NYPLYVKTTI SCAFITSMIP TMMFIHTGQE MIISNWHWLT IQTLKLSLSF KMDFFSMMF VPVALFVTWS IMEFSMWYMH SDPNINQFFK YLLLFLITML ILVTANNLFQ LFIGWEGVGI MSFLLIGWWY G RTDANTAA ...String:
MNLFSSLTLV TLILLTMPIA AINFNTHKFT NYPLYVKTTI SCAFITSMIP TMMFIHTGQE MIISNWHWLT IQTLKLSLSF KMDFFSMMF VPVALFVTWS IMEFSMWYMH SDPNINQFFK YLLLFLITML ILVTANNLFQ LFIGWEGVGI MSFLLIGWWY G RTDANTAA LQAILYNRIG DIGFILAMAW FLINLNTWDL QQIFMLNPND SNLPLMGLIL AATGKSAQFG LHPWLPSAME GP TPVSALL HSSTMVVAGI FLLIRFYPLT ENNKFGQSIM LCLGAMTTLF TAMCALTQND IKKIIAFSTS SQLGLMMVTI GIN QPHLAF LHICTHAFFK AMLFMCSGSI IHSLNDEQDI RKMGGLFKAM PFTTTALIIG SLALTGMPFL TGFYSKDLII ESAN TSYTN AWALLMTLVA TSFTAIYSTR IIFFALLGQP RFPTLININE NNPFLINSIK RLLIGSLFAG FIISNNIPPM TIPQM TMPH YLKMTALTVT ILGFILALEI SNTTHYLKFN YPSNTFKFSN LLGYYPTIMH RLTPYMNLTM SQKSASSLLD LIWLET ILP KTISLAQMKM STTITSQKGL IKLYFLSFLI TILIST

UniProtKB: NADH-ubiquinone oxidoreductase chain 5

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Macromolecule #14: Mitochondrial complex I, ND6 subunit

MacromoleculeName: Mitochondrial complex I, ND6 subunit / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 19.126619 KDa
SequenceString:
MMTYIVFILS IIFVMGFVGF SSKPSPIYGG LGLIVSGGVG CGIVLNFGGS FLGLMVFLIY LGGMMVVFGY TTAMATEQYP EVWVSNKVV LGTFITGLLM EFLMVYYVLK DKEVEIVFKF NGMGDWVIYD TGDSGFFSEE AMGIAALYSY GTWLVIVTGW S LLIGVVVI MEITRGN

UniProtKB: NADH-ubiquinone oxidoreductase chain 6

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Macromolecule #15: Mitochondrial complex I, 10 kDa subunit

MacromoleculeName: Mitochondrial complex I, 10 kDa subunit / type: protein_or_peptide / ID: 15 / Details: NCBIprot XP_011991231.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 8.423314 KDa
SequenceString:
SAESGKNEKG LPPNPKKQSP PKKPASAAPT EPFDNTTYKN LQHHDYSTYT FLDLNLDLSK FRMPQPSSGR ESPRH

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Macromolecule #16: Mitochondrial complex I, 13 kDa subunit

MacromoleculeName: Mitochondrial complex I, 13 kDa subunit / type: protein_or_peptide / ID: 16 / Details: NCBIprot XP_011980592.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 10.651796 KDa
SequenceString:
GVRTSPTGEK VTHTGQVYDD EDYRRVRFVG RQKEVNENFA IDLIAEQPVS QVGSRVISCD GGGGALGHPR VYINLDKETK TGTCGYCGL QFRQQHH

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Macromolecule #17: Mitochondrial complex I, 18 kDa subunit

MacromoleculeName: Mitochondrial complex I, 18 kDa subunit / type: protein_or_peptide / ID: 17 / Details: NCBIprot XP_004017046.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 15.3753 KDa
SequenceString:
AQDQTRDTQL ITVDEKLDIT TLTGVPEEHI KTRKARIFVP ARNNMQSGVN NTKKWKMEFD TRERWENPLM GWASTADPLS NLVLTFSTK EDAIAFAEKN GWSYDVEERK VPKPKSKSYG ANFSWNKRTR VSTK

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Macromolecule #18: Mitochondrial complex I, 39 kDa subunit

MacromoleculeName: Mitochondrial complex I, 39 kDa subunit / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 38.660488 KDa
SequenceString: LHHAVIPHGK GGRSSVSGIV ATVFGATGFL GRYLVNHLGR MGSQVIVPYR CEPYDTMHLR PMGDLGQIIF MDWNGRDKDS IRRAVEHSN VVINLVGREW ETKNFDFEDV FVKIPQAIAQ VSKEAGVEKF IHISHLNADI KSSSKYLRNK AVGEKEVRET F PEATIIKP ...String:
LHHAVIPHGK GGRSSVSGIV ATVFGATGFL GRYLVNHLGR MGSQVIVPYR CEPYDTMHLR PMGDLGQIIF MDWNGRDKDS IRRAVEHSN VVINLVGREW ETKNFDFEDV FVKIPQAIAQ VSKEAGVEKF IHISHLNADI KSSSKYLRNK AVGEKEVRET F PEATIIKP ADIFGREDRF LNYFANIRWF GGVPLISLGK KTVKQPVYIV DVTKGIINAI KDPDARGKTF AFVGPNRYLL FD LVQYVFA VAHRPFLPYP MPHFAYRWIG RLFEISPFEP WTTRDKVERI HTTDRTLPHL PGLEDLGVQA TPLELKAIEV LRR HRTYRW LSSEIEDVQP AK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

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Macromolecule #19: Mitochondrial complex I, B8 subunit

MacromoleculeName: Mitochondrial complex I, B8 subunit / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 10.966627 KDa
SequenceString:
AAAAAIRGVR GKLGLREIRI HLCQRSPGSQ GVRDFIEKRY VELKKANPDL PILIRECSDV QPKLWARYAF GQEKNVSLNN FSADQVTRA LENVLSSKA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

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Macromolecule #20: Mitochondrial complex I, B13 subunit

MacromoleculeName: Mitochondrial complex I, B13 subunit / type: protein_or_peptide / ID: 20 / Details: NCBIprot XP_004005022.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 13.156311 KDa
SequenceString:
AGLLKKTTGL VGLAVCETPH ERLKILYTKI LDVLGHIPKN AAYRKYTEQI TNEKLSIVKA EPDVKKLEEQ LQGGQIEEVI LQAENELSL ARKMIQWKPW EPLVEEPPAS QWKWPI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

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Macromolecule #21: Mitochondrial complex I, B14 subunit

MacromoleculeName: Mitochondrial complex I, B14 subunit / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 14.923245 KDa
SequenceString:
AASGLRQAAV AASTSVKPIF SRDMNEAKRR VRELYRAWYR EVPNTVHLFQ LDISVKQGRD KVREMFKKNA HVTDPRVVDL LVIKGKMEL EETINVWKQR THVMRFFHET EAPRPKDFLS KFYVGHDP

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

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Macromolecule #22: Mitochondrial complex I, B14.5a subunit

MacromoleculeName: Mitochondrial complex I, B14.5a subunit / type: protein_or_peptide / ID: 22 / Details: NCBIprot XP_004008614.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 12.399201 KDa
SequenceString:
ASATRLIQGL RNWASGRDLQ AKLQLRYQEI SKRTQPPPKL PVGPSHRLSN NYYCARDGRR EAMPPSIVMS SQKVLVAGKP AESSAVAAS EKKAVSPAPP IKRWELSQDE PYL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

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Macromolecule #23: Mitochondrial complex I, B17.2 subunit

MacromoleculeName: Mitochondrial complex I, B17.2 subunit / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 17.115508 KDa
SequenceString:
MELLQVLKRG LQQVSGHGGL RGYLRVLFRA NDVRVGTLVG EDKYGNKYYE DNKQFFGRHR WVIYTTEMNG KNTFWDVDGS MVPPEWHRW LHCMTDDPPT VKPPTARKFI WTNHKFNLSG TPQQYVPYST TRKKIQEWVP PSTPYK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

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Macromolecule #24: Acyl carrier protein

MacromoleculeName: Acyl carrier protein / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 10.119541 KDa
SequenceString:
SDAPPLTLEG IKDRVLYVLK LYDKIDPEKL SVNSHFMKDL GLDSLDQVEI IMAMEDEFGF EIPDIDAEKL MCPQEIVDYI ADKKDVYE

UniProtKB: Acyl carrier protein

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Macromolecule #25: Mitochondrial complex I, 42 kDa subunit

MacromoleculeName: Mitochondrial complex I, 42 kDa subunit / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 36.803762 KDa
SequenceString: LQYGPLAYIL GEKTTKKMTE NSKLITVDGN ICSGKSKLAK EVAEKLGLKH FPEAGIHYAD STTGDGKPLP VRFSGNCSLE KFYDDPKSN DGNSYRLQAW LYASRLLQYA DALEHLLSTG QGVVLERSIY SDFVFLEAMY RQGFIRKQCV DHYNQVKKVT V CEYLPPHV ...String:
LQYGPLAYIL GEKTTKKMTE NSKLITVDGN ICSGKSKLAK EVAEKLGLKH FPEAGIHYAD STTGDGKPLP VRFSGNCSLE KFYDDPKSN DGNSYRLQAW LYASRLLQYA DALEHLLSTG QGVVLERSIY SDFVFLEAMY RQGFIRKQCV DHYNQVKKVT V CEYLPPHV VIYVDVPVSE VQSRIQKKGN PHEMKITSAY LQDIENVYKG TFLPEMSEKC EVLQYSAWEA EDAEKVVEDI QY LKYDKGP WLDQDDRKLH NLRMLVQDKL EVLNYTSIPV FLPEVTIGAH QSDRVFQEFT ELPGRKYRAG YNEDVGDKWI WLK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

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Macromolecule #26: Mitochondrial complex I, 15 kDa subunit

MacromoleculeName: Mitochondrial complex I, 15 kDa subunit / type: protein_or_peptide / ID: 26 / Details: NCBIprot XP_004001873.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 12.474397 KDa
SequenceString:
PFFDVQKKLG VDLDHWMTIQ SAEQPHRIPA RCHAFEKEWI ECAHGIGSIR AEKECKIEFE DFRECLLRQK TMKRLNAIKR QRDKLIKEG KYTPPPHHSG QEDLRP

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Macromolecule #27: Mitochondrial complex I, B9 subunit

MacromoleculeName: Mitochondrial complex I, B9 subunit / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 9.194655 KDa
SequenceString:
APRVAAFLKN VWAKEPVLVA SFAIAGLAVI LPTLSPYTKY SLMINRATPY NYPVPLRDDG NMPDVPSHPQ DPQGPSLEWL KRL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

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Macromolecule #28: Mitochondrial complex I, B12 subunit

MacromoleculeName: Mitochondrial complex I, B12 subunit / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 11.015482 KDa
SequenceString:
AHGHGHEHGP SKMELPDYKQ WKIEGTPLET VQEKLAARGL RDPWGRNEAW RYMGGFANNV SFVGALLKGF KWGFAAFVVA VGAEYYLES QKKDKKHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

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Macromolecule #29: Mitochondrial complex I, B14.5b subunit

MacromoleculeName: Mitochondrial complex I, B14.5b subunit / type: protein_or_peptide / ID: 29 / Details: NCBIprot XP_004019479.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 14.23142 KDa
SequenceString:
MMTGRQARAP LQFLPDEARS LPPPKLTDPR LAYIGFLGYC SGLIDNAIRR RPVLSAGLHR QFLYITSFVF VGYYLLKRQD YMYAVRDHD MFSYIKSHPE DFPEKDKKTY REVFEEFHPV R

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Macromolecule #30: Mitochondrial complex I, B15 subunit

MacromoleculeName: Mitochondrial complex I, B15 subunit / type: protein_or_peptide / ID: 30 / Details: NCBIprot XP_004003003.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 14.975999 KDa
SequenceString:
SFPKYEPSRL ASLPTTLDPA EYDISSETRK AQAERLAIRS RLKREYQLQY NDPSRRGVVE DPALIRWTCA RSANVYPNFR PNTKTSLLG ALFGIGPLIF WYYVFKTDRD RKEKLIQEGK LDRTFNISY

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Macromolecule #31: Mitochondrial complex I, B16.6 subunit

MacromoleculeName: Mitochondrial complex I, B16.6 subunit / type: protein_or_peptide / ID: 31 / Details: NCBIprot XP_004008450.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 16.635264 KDa
SequenceString:
AASKVKQDMP PVGGYGPIDY KRNLPRRGLS GYSMFAVGIG ALLFGYWSMM RWNRERRRLQ IEDFEARIAL MPLLQAEKDR RVLQMLREN LEEEATIMKD VPGWKVGESV FHTTRWVTPM MGELYGLRTG EEILSSTYGF IWYT

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Macromolecule #32: Mitochondrial complex I, B17 subunit

MacromoleculeName: Mitochondrial complex I, B17 subunit / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 15.433929 KDa
SequenceString:
SGYTPDEKLR LQQLRELRRR WLKDQELSPR EPVLPPRRVS PVERFWNKFL QDGALWKNVI YKTYRHSIFA FTHVLIPVWI IHYYLKYHV TTKPYTIVEK KPRIFPGDTI LETGEVIPPM KEFPDQHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

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Macromolecule #33: Mitochondrial complex I, B18 subunit

MacromoleculeName: Mitochondrial complex I, B18 subunit / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 16.28261 KDa
SequenceString:
GAHLARRYLG DASVEPEPLR MPTFPPDYGF PERKEREMVA TQQEMNDAQL VLQQRDYCAH YLIRFLKCKR DSFPNFLACK HEQHDWDYC EHLDYVKRMK EFERERRLLQ RKKRREQREA DMAKGLGPGE VAPEVAL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

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Macromolecule #34: Mitochondrial complex I, B22 subunit

MacromoleculeName: Mitochondrial complex I, B22 subunit / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 21.648715 KDa
SequenceString:
AFLPSAAYLT HQQKVLQLYK RALRHLESRC VHRDKYRYFA CLLRARFDEH KNEKDMVKAT QLLREAEKEF WHGQHPQPYI FPESPGGTS YERYECYKVP EWCLDDWHPS EKAMYPDYFA KREQWKKLRR ESWEREVKQL QEETPVGGPR TEALPPARKQ G DLPPLWWH IVTRPRERPM

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

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Macromolecule #35: Mitochondrial complex I, AGGG subunit

MacromoleculeName: Mitochondrial complex I, AGGG subunit / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 8.500289 KDa
SequenceString:
AGGGAHIEPR YRQFPQLTRS QVIQAEFFSA TMWFWILWRF WHDSDAVLGH FPYPDPSQWT DEELGIPPDD ED

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial

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Macromolecule #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mito...

MacromoleculeName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 18.81807 KDa
SequenceString:
ASHITKDMLP GPYPKTPEER AAAAKKYNMR VEDYEPYPDD GMGYGDYPKL PDRSQQERDP WYDWDHPDLR LNWGEPMHWD LDMYIRNRV DTSPTPVNWN LMCKHLFGFV AFMLFMFWVG ETYPTYQPVG PKQYPYNNLY LERGGDPNKE PEPVVHYEI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

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Macromolecule #37: Mitochondrial complex I, ESSS subunit

MacromoleculeName: Mitochondrial complex I, ESSS subunit / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 14.455033 KDa
SequenceString:
ESSSSRAVIA PSTLAGKRPS EPTLRWQEDP EPEDENLYEK NPDSHGYDKD PAVDVWNMRV VFFFGFSIVL VLGSTFVAYL PDYRMQEWA RREAERLVKY REAHGLPLME SNCFDPSKIQ LPEDED

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial

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Macromolecule #38: Mitochondrial complex I, KFYI subunit

MacromoleculeName: Mitochondrial complex I, KFYI subunit / type: protein_or_peptide / ID: 38 / Details: NCBIprot XP_004017292.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 5.808645 KDa
SequenceString:
KFYIQEPPHG SPNWLKVGLT LGTSAFLWIY LIKQHNEDVL EYKRRNGLE

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Macromolecule #39: Mitochondrial complex I, MNLL subunit

MacromoleculeName: Mitochondrial complex I, MNLL subunit / type: protein_or_peptide / ID: 39 / Details: NCBIprot XP_004018002.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 6.930083 KDa
SequenceString:
MNLLQVVRDH WIHVLVPVGF VFGYYLDRKN DEKLAAFRNK SLLYKRELKP NEEVTWK

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Macromolecule #40: Mitochondrial complex I, MWFE subunit

MacromoleculeName: Mitochondrial complex I, MWFE subunit / type: protein_or_peptide / ID: 40 / Details: NCBIprot NP_001305903.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 8.211519 KDa
SequenceString:
MWFEVLPGIA VMGVCLFIPG MATARIHRFS NGGREKRVAH YSYQWYLMER DRRVSGVNRY YVSKGLENID

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Macromolecule #41: Mitochondrial complex I, PDSW subunit

MacromoleculeName: Mitochondrial complex I, PDSW subunit / type: protein_or_peptide / ID: 41 / Details: NCBIprot XP_011999786.1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 20.880752 KDa
SequenceString:
PDSWDKDVYP EPPRRTPAPS PQTSLPNPIT YLTKAFDLLV DRPVTLVREF IERQHAKNKY YYYHREFRRV PDITECHEKD VLCMFEAEM QWKRDYKVDQ EIVNIIQERL KACQQREGES HRQNCAKELQ QFTQVVKAYQ DRYHDLGAHY SARKCLAKQK Q RMLAERKA TKEAAAA

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Macromolecule #42: Mitochondrial complex I, PGIV subunit

MacromoleculeName: Mitochondrial complex I, PGIV subunit / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 20.008014 KDa
SequenceString:
PGIVELPSLE DLKVQEVKVS SSVLKAAAHH YGAQCDKPNK EFMLCRWEEK DPRRCLEEGK LVNQCALEFF RQIKRHCAEP FTEYWTCID YSGLQLFRRC RKEQAQFDKC VLDKLGWVRP DLGELSKVTK VKTDRPLPEN PYHSRARPEP NPEVEGDLKP A RHGSRLFF WTM

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

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Macromolecule #43: Mitochondrial complex I, SGDH subunit

MacromoleculeName: Mitochondrial complex I, SGDH subunit / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 16.714369 KDa
SequenceString:
SGDHGKRLFI IKPSGFYDKR FLKLLRFYIL LTGIPVVIGI TLINVFIGEA ELAEIPEGYV PEHWEYFKHP ISRWIARTFF DAPEKNYER TMAILQIESE KAELRLKELE VRRLMRAKGD GPWFQYPTID KALIDHSPKA TPDN

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

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Macromolecule #44: Mitochondrial complex I, B14.7 subunit

MacromoleculeName: Mitochondrial complex I, B14.7 subunit / type: protein_or_peptide / ID: 44 / Details: UniProt W5PAR2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 10.145497 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #45: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 45 / Number of copies: 6 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Macromolecule #46: FLAVIN MONONUCLEOTIDE

MacromoleculeName: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 46 / Number of copies: 1 / Formula: FMN
Molecular weightTheoretical: 456.344 Da
Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

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Macromolecule #47: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 47 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

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Macromolecule #48: 1,2-Distearoyl-sn-glycerophosphoethanolamine

MacromoleculeName: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 48 / Number of copies: 4 / Formula: 3PE
Molecular weightTheoretical: 748.065 Da
Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM

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Macromolecule #49: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

MacromoleculeName: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 49 / Number of copies: 4 / Formula: PC1
Molecular weightTheoretical: 790.145 Da
Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

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Macromolecule #50: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 50 / Number of copies: 4 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

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Macromolecule #51: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 51 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #52: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

MacromoleculeName: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
type: ligand / ID: 52 / Number of copies: 1 / Formula: NDP
Molecular weightTheoretical: 745.421 Da
Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

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Macromolecule #53: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

MacromoleculeName: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
type: ligand / ID: 53 / Number of copies: 1 / Formula: ZMP
Molecular weightTheoretical: 568.704 Da
Chemical component information

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

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Macromolecule #54: 4'-PHOSPHOPANTETHEINE

MacromoleculeName: 4'-PHOSPHOPANTETHEINE / type: ligand / ID: 54 / Number of copies: 1 / Formula: PNS
Molecular weightTheoretical: 358.348 Da
Chemical component information

ChemComp-PNS:
4'-PHOSPHOPANTETHEINE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration5 mg/mL
BufferpH: 7.4
Component:
ConcentrationFormulaName
100.0 mMNaClSodium Chloride
20.0 mMHEPES
2.0 mMEDTA
1.5 %Glycerol
0.2 %Brij-35
GridModel: Quantifoil R0.6/1 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 120 sec. / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV / Details: 34s blotting.

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Digitization - Frames/image: 1-7 / Number grids imaged: 2 / Number real images: 2600 / Average exposure time: 1.0 sec. / Average electron dose: 26.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 100720 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 59000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 241000
Startup modelType of model: EMDB MAP
EMDB ID:

2676

Final reconstructionNumber classes used: 1 / Resolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 82000
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.4)
Final 3D classificationSoftware - Name: RELION (ver. 1.4)

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Atomic model buiding 1

Initial modelPDB ID:

4hea


Chain - Source name: PDB / Chain - Initial model type: experimental model
Details: For the fourteen core subunits we used as a starting point our previous structure from T. thermophilus (PDB 4HEA) with sequence adjusted to ovine and side-chains rebuilt in SQWRL4. These are ...Details: For the fourteen core subunits we used as a starting point our previous structure from T. thermophilus (PDB 4HEA) with sequence adjusted to ovine and side-chains rebuilt in SQWRL4. These are chains 1, 2, 3, 4, 5, 6, 9, H, A, J, K, N, M and L (with the same chain IDs as in 4HEA). For the remaining supernumerary subunits it was mostly de novo building with some help from homology modeling, which in most cases was not very useful due to low sequence homology. In few cases models produced by Phyre2 server were useful and were used as a starting point, due to the presence of templates with high coverage and confidence: 42kDa (chain k) - PDB ID 1P6X (xray) 39kDa (chain d) - PDB ID 3WJ7 (xray) SDAPs (chains j and X) - PDB ID 2DNW (NMR) B8 (chain e) - PDB ID 1S3A (NMR)
RefinementSpace: REAL / Protocol: AB INITIO MODEL
Output model

PDB-5lnk:
Entire ovine respiratory complex I

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