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- EMDB-4093: Entire ovine respiratory complex I, Combined Map -

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Entry
Database: EMDB / ID: 4093
TitleEntire ovine respiratory complex I, Combined Map
Map data
SampleMitochondrial complex I
  • (Mitochondrial complex I, ...) x 42
  • Acyl carrier protein
  • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
  • (ligand) x 10
Function / homologyNAD(P)H-quinone oxidoreductase subunit D/H / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / NADH dehydrogenase subunit 2, C-terminal / NADH dehydrogenase subunit 5, C-terminal / NADH ubiquinone oxidoreductase, F subunit / NADH-ubiquinone oxidoreductase 51kDa subunit, FMN-binding domain / NADH:ubiquinone oxidoreductase, MNLL subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH:ubiquinone oxidoreductase, subunit G ...NAD(P)H-quinone oxidoreductase subunit D/H / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / NADH dehydrogenase subunit 2, C-terminal / NADH dehydrogenase subunit 5, C-terminal / NADH ubiquinone oxidoreductase, F subunit / NADH-ubiquinone oxidoreductase 51kDa subunit, FMN-binding domain / NADH:ubiquinone oxidoreductase, MNLL subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH:ubiquinone oxidoreductase, subunit G / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NAD(P)-binding domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, metazoa / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / CHCH / NADH-quinone oxidoreductase, chain M/4 / 4Fe-4S ferredoxin-type, iron-sulphur binding domain / Complex 1 LYR protein / Phosphopantetheine attachment site / Molybdopterin oxidoreductase / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / Molybdopterin oxidoreductase, 4Fe-4S domain / Ribosomal protein/NADH dehydrogenase domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH:ubiquinone oxidoreductase, B18 subunit / NADH-quinone oxidoreductase, chain I / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / Phosphopantetheine binding ACP domain / GRIM-19 / NADH:ubiquinone oxidoreductase, subunit b14.5b / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH:ubiquinone oxidoreductase subunit B14.5a / NADH dehydrogenase, subunit C / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / NADH:ubiquinone oxidoreductase-like, 20kDa subunit / NADH-quinone oxidoreductase, subunit D superfamily / Thioredoxin-like superfamily / NAD(P)-binding domain superfamily / ACP-like superfamily / NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain superfamily / NADH-ubiquinone oxidoreductase 51kDa subunit, FMN-binding domain superfamily / NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily / NADH:ubiquinone oxidoreductase, subunit 3 superfamily / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH dehydrogenase / Respiratory-chain NADH dehydrogenase, 30 Kd subunit / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / Proton-conducting membrane transporter / Molybdopterin oxidoreductase / 2Fe-2S ferredoxin-like superfamily / Deoxynucleoside kinase domain / NADH:ubiquinone oxidoreductase, subunit 1, conserved site / NADH:ubiquinone oxidoreductase, subunit G, iron-sulphur binding / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, ESSS subunit / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / NADH-ubiquinone oxidoreductase, subunit 10 / Zinc finger, CHCC-type / Soluble ligand binding domain / NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain / [NiFe]-hydrogenase, large subunit / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH-ubiquinone oxidoreductase flavoprotein 3 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 / P-loop containing nucleoside triphosphate hydrolase / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-plastoquinone oxidoreductase, chain 5 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / 4Fe-4S dicluster domain / NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 beta subcomplex subunit 2 / NAD(P)H-binding / NADH-ubiquinone oxidoreductase-F iron-sulfur binding region / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / SLBB domain / Zinc-finger domain / NADH-ubiquinone oxidoreductase subunit 10 / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / Phosphopantetheine attachment site. / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH-ubiquinone oxidoreductase subunit G, C-terminal / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / Complex I biogenesis
Function and homology information
SourceOvis aries (sheep)
Methodsingle particle reconstruction / cryo EM / 3.9 Å resolution
AuthorsFiedorczuk K / Letts JA / Sazanov LA
CitationJournal: Nature / Year: 2016
Title: Atomic structure of the entire mammalian mitochondrial complex I.
Authors: Karol Fiedorczuk / James A Letts / Gianluca Degliesposti / Karol Kaszuba / Mark Skehel / Leonid A Sazanov
Validation ReportPDB-ID: 5lnk

SummaryFull reportAbout validation report
DateDeposition: Oct 28, 2016 / Header (metadata) release: Nov 23, 2016 / Map release: Nov 23, 2016 / Last update: Aug 30, 2017

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.15
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.15
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: : PDB-5lnk
  • Surface level: 0.15
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

Fileemd_4093.map.gz (map file in CCP4 format, 15207 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
129 pix
1.39 Å/pix.
= 179.31 Å
141 pix
1.39 Å/pix.
= 195.99 Å
209 pix
1.39 Å/pix.
= 290.51 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

(generated in cubic-lattice coordinate)

Voxel sizeX=Y=Z: 1.39 Å
Density
Contour Level:0.15 (by author), 0.15 (movie #1):
Minimum - Maximum-0.5494037 - 1.3139707
Average (Standard dev.)0.0089633055 (0.05093917)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderZYX
Dimensions141209129
Origin000
Limit140208128
Spacing129141209
CellA: 179.31 Å / B: 195.99 Å / C: 290.51 Å
α=β=γ: 90 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.391.391.39
M x/y/z129141209
origin x/y/z0.0000.0000.000
length x/y/z179.310195.990290.510
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ129141209
MAP C/R/S321
start NC/NR/NS000
NC/NR/NS209141129
D min/max/mean-0.5491.3140.009

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Supplemental data

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Sample components

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Entire Mitochondrial complex I

EntireName: Mitochondrial complex I / Number of components: 55
MassExperimental: 970 kDa

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Component #1: protein, Mitochondrial complex I

ProteinName: Mitochondrial complex I / Recombinant expression: No
MassExperimental: 970 kDa
SourceSpecies: Ovis aries (sheep)

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Component #2: protein, Mitochondrial complex I, 51 kDa subunit

ProteinName: Mitochondrial complex I, 51 kDa subunit / Recombinant expression: No
MassTheoretical: 48.678281 kDa
SourceSpecies: Ovis aries (sheep)

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Component #3: protein, Mitochondrial complex I, 24 kDa subunit

ProteinName: Mitochondrial complex I, 24 kDa subunit / Recombinant expression: No
MassTheoretical: 23.840428 kDa
SourceSpecies: Ovis aries (sheep)

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Component #4: protein, Mitochondrial complex I, 75 kDa subunit

ProteinName: Mitochondrial complex I, 75 kDa subunit / Recombinant expression: No
MassTheoretical: 77.031156 kDa
SourceSpecies: Ovis aries (sheep)

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Component #5: protein, Mitochondrial complex I, 49 kDa subunit

ProteinName: Mitochondrial complex I, 49 kDa subunit / Recombinant expression: No
MassTheoretical: 47.120078 kDa
SourceSpecies: Ovis aries (sheep)

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Component #6: protein, Mitochondrial complex I, 30 kDa subunit

ProteinName: Mitochondrial complex I, 30 kDa subunit / Recombinant expression: No
MassTheoretical: 26.441793 kDa
SourceSpecies: Ovis aries (sheep)

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Component #7: protein, Mitochondrial complex I, PSST subunit

ProteinName: Mitochondrial complex I, PSST subunit / Details: NCBIProt XP_012033846.2 / Recombinant expression: No
MassTheoretical: 20.104531 kDa
SourceSpecies: Ovis aries (sheep)

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Component #8: protein, Mitochondrial complex I, TYKY subunit

ProteinName: Mitochondrial complex I, TYKY subunit / Details: NCBIprot XP_011972879.1 / Recombinant expression: No
MassTheoretical: 20.219947 kDa
SourceSpecies: Ovis aries (sheep)

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Component #9: protein, Mitochondrial complex I, ND1 subunit

ProteinName: Mitochondrial complex I, ND1 subunit / Recombinant expression: No
MassTheoretical: 35.884902 kDa
SourceSpecies: Ovis aries (sheep)

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Component #10: protein, Mitochondrial complex I, ND2 subunit

ProteinName: Mitochondrial complex I, ND2 subunit / Recombinant expression: No
MassTheoretical: 39.149805 kDa
SourceSpecies: Ovis aries (sheep)

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Component #11: protein, Mitochondrial complex I, ND3 subunit

ProteinName: Mitochondrial complex I, ND3 subunit / Recombinant expression: No
MassTheoretical: 13.10652 kDa
SourceSpecies: Ovis aries (sheep)

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Component #12: protein, Mitochondrial complex I, ND4 subunit

ProteinName: Mitochondrial complex I, ND4 subunit / Recombinant expression: No
MassTheoretical: 52.058734 kDa
SourceSpecies: Ovis aries (sheep)

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Component #13: protein, Mitochondrial complex I, ND4L subunit

ProteinName: Mitochondrial complex I, ND4L subunit / Recombinant expression: No
MassTheoretical: 10.840228 kDa
SourceSpecies: Ovis aries (sheep)

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Component #14: protein, Mitochondrial complex I, ND5 subunit

ProteinName: Mitochondrial complex I, ND5 subunit / Recombinant expression: No
MassTheoretical: 67.554945 kDa
SourceSpecies: Ovis aries (sheep)

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Component #15: protein, Mitochondrial complex I, ND6 subunit

ProteinName: Mitochondrial complex I, ND6 subunit / Recombinant expression: No
MassTheoretical: 19.126619 kDa
SourceSpecies: Ovis aries (sheep)

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Component #16: protein, Mitochondrial complex I, 10 kDa subunit

ProteinName: Mitochondrial complex I, 10 kDa subunit / Details: NCBIprot XP_011991231.1 / Recombinant expression: No
MassTheoretical: 8.423314 kDa
SourceSpecies: Ovis aries (sheep)

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Component #17: protein, Mitochondrial complex I, 13 kDa subunit

ProteinName: Mitochondrial complex I, 13 kDa subunit / Details: NCBIprot XP_011980592.1 / Recombinant expression: No
MassTheoretical: 10.651796 kDa
SourceSpecies: Ovis aries (sheep)

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Component #18: protein, Mitochondrial complex I, 18 kDa subunit

ProteinName: Mitochondrial complex I, 18 kDa subunit / Details: NCBIprot XP_004017046.1 / Recombinant expression: No
MassTheoretical: 15.3753 kDa
SourceSpecies: Ovis aries (sheep)

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Component #19: protein, Mitochondrial complex I, 39 kDa subunit

ProteinName: Mitochondrial complex I, 39 kDa subunit / Recombinant expression: No
MassTheoretical: 38.660488 kDa
SourceSpecies: Ovis aries (sheep)

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Component #20: protein, Mitochondrial complex I, B8 subunit

ProteinName: Mitochondrial complex I, B8 subunit / Recombinant expression: No
MassTheoretical: 10.966627 kDa
SourceSpecies: Ovis aries (sheep)

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Component #21: protein, Mitochondrial complex I, B13 subunit

ProteinName: Mitochondrial complex I, B13 subunit / Details: NCBIprot XP_004005022.1 / Recombinant expression: No
MassTheoretical: 13.156311 kDa
SourceSpecies: Ovis aries (sheep)

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Component #22: protein, Mitochondrial complex I, B14 subunit

ProteinName: Mitochondrial complex I, B14 subunit / Recombinant expression: No
MassTheoretical: 14.923245 kDa
SourceSpecies: Ovis aries (sheep)

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Component #23: protein, Mitochondrial complex I, B14.5a subunit

ProteinName: Mitochondrial complex I, B14.5a subunit / Details: NCBIprot XP_004008614.1 / Recombinant expression: No
MassTheoretical: 12.399201 kDa
SourceSpecies: Ovis aries (sheep)

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Component #24: protein, Mitochondrial complex I, B17.2 subunit

ProteinName: Mitochondrial complex I, B17.2 subunit / Recombinant expression: No
MassTheoretical: 17.115508 kDa
SourceSpecies: Ovis aries (sheep)

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Component #25: protein, Acyl carrier protein

ProteinName: Acyl carrier protein / Recombinant expression: No
MassTheoretical: 10.119541 kDa
SourceSpecies: Ovis aries (sheep)

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Component #26: protein, Mitochondrial complex I, 42 kDa subunit

ProteinName: Mitochondrial complex I, 42 kDa subunit / Recombinant expression: No
MassTheoretical: 36.803762 kDa
SourceSpecies: Ovis aries (sheep)

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Component #27: protein, Mitochondrial complex I, 15 kDa subunit

ProteinName: Mitochondrial complex I, 15 kDa subunit / Details: NCBIprot XP_004001873.1 / Recombinant expression: No
MassTheoretical: 12.474397 kDa
SourceSpecies: Ovis aries (sheep)

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Component #28: protein, Mitochondrial complex I, B9 subunit

ProteinName: Mitochondrial complex I, B9 subunit / Recombinant expression: No
MassTheoretical: 9.194655 kDa
SourceSpecies: Ovis aries (sheep)

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Component #29: protein, Mitochondrial complex I, B12 subunit

ProteinName: Mitochondrial complex I, B12 subunit / Recombinant expression: No
MassTheoretical: 11.015482 kDa
SourceSpecies: Ovis aries (sheep)

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Component #30: protein, Mitochondrial complex I, B14.5b subunit

ProteinName: Mitochondrial complex I, B14.5b subunit / Details: NCBIprot XP_004019479.1 / Recombinant expression: No
MassTheoretical: 14.23142 kDa
SourceSpecies: Ovis aries (sheep)

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Component #31: protein, Mitochondrial complex I, B15 subunit

ProteinName: Mitochondrial complex I, B15 subunit / Details: NCBIprot XP_004003003.1 / Recombinant expression: No
MassTheoretical: 14.975999 kDa
SourceSpecies: Ovis aries (sheep)

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Component #32: protein, Mitochondrial complex I, B16.6 subunit

ProteinName: Mitochondrial complex I, B16.6 subunit / Details: NCBIprot XP_004008450.1 / Recombinant expression: No
MassTheoretical: 16.635264 kDa
SourceSpecies: Ovis aries (sheep)

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Component #33: protein, Mitochondrial complex I, B17 subunit

ProteinName: Mitochondrial complex I, B17 subunit / Recombinant expression: No
MassTheoretical: 15.433929 kDa
SourceSpecies: Ovis aries (sheep)

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Component #34: protein, Mitochondrial complex I, B18 subunit

ProteinName: Mitochondrial complex I, B18 subunit / Recombinant expression: No
MassTheoretical: 16.28261 kDa
SourceSpecies: Ovis aries (sheep)

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Component #35: protein, Mitochondrial complex I, B22 subunit

ProteinName: Mitochondrial complex I, B22 subunit / Recombinant expression: No
MassTheoretical: 21.648715 kDa
SourceSpecies: Ovis aries (sheep)

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Component #36: protein, Mitochondrial complex I, AGGG subunit

ProteinName: Mitochondrial complex I, AGGG subunit / Recombinant expression: No
MassTheoretical: 8.500289 kDa
SourceSpecies: Ovis aries (sheep)

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Component #37: protein, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subuni...

ProteinName: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
Recombinant expression: No
MassTheoretical: 18.81807 kDa
SourceSpecies: Ovis aries (sheep)

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Component #38: protein, Mitochondrial complex I, ESSS subunit

ProteinName: Mitochondrial complex I, ESSS subunit / Recombinant expression: No
MassTheoretical: 14.455033 kDa
SourceSpecies: Ovis aries (sheep)

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Component #39: protein, Mitochondrial complex I, KFYI subunit

ProteinName: Mitochondrial complex I, KFYI subunit / Details: NCBIprot XP_004017292.1 / Recombinant expression: No
MassTheoretical: 5.808645 kDa
SourceSpecies: Ovis aries (sheep)

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Component #40: protein, Mitochondrial complex I, MNLL subunit

ProteinName: Mitochondrial complex I, MNLL subunit / Details: NCBIprot XP_004018002.1 / Recombinant expression: No
MassTheoretical: 6.930083 kDa
SourceSpecies: Ovis aries (sheep)

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Component #41: protein, Mitochondrial complex I, MWFE subunit

ProteinName: Mitochondrial complex I, MWFE subunit / Details: NCBIprot NP_001305903.1 / Recombinant expression: No
MassTheoretical: 8.211519 kDa
SourceSpecies: Ovis aries (sheep)

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Component #42: protein, Mitochondrial complex I, PDSW subunit

ProteinName: Mitochondrial complex I, PDSW subunit / Details: NCBIprot XP_011999786.1 / Recombinant expression: No
MassTheoretical: 20.880752 kDa
SourceSpecies: Ovis aries (sheep)

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Component #43: protein, Mitochondrial complex I, PGIV subunit

ProteinName: Mitochondrial complex I, PGIV subunit / Recombinant expression: No
MassTheoretical: 20.008014 kDa
SourceSpecies: Ovis aries (sheep)

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Component #44: protein, Mitochondrial complex I, SGDH subunit

ProteinName: Mitochondrial complex I, SGDH subunit / Recombinant expression: No
MassTheoretical: 16.714369 kDa
SourceSpecies: Ovis aries (sheep)

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Component #45: protein, Mitochondrial complex I, B14.7 subunit

ProteinName: Mitochondrial complex I, B14.7 subunit / Details: UniProt W5PAR2 / Recombinant expression: No
MassTheoretical: 10.145497 kDa
SourceSpecies: Ovis aries (sheep)

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Component #46: ligand, IRON/SULFUR CLUSTER

LigandName: IRON/SULFUR CLUSTERIron–sulfur cluster / Number of Copies: 6 / Recombinant expression: No
MassTheoretical: 0.35164 kDa

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Component #47: ligand, FLAVIN MONONUCLEOTIDE

LigandName: FLAVIN MONONUCLEOTIDE / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.456344 kDa

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Component #48: ligand, FE2/S2 (INORGANIC) CLUSTER

LigandName: FE2/S2 (INORGANIC) CLUSTER / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 0.17582 kDa

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Component #49: ligand, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE

LigandName: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE / Number of Copies: 4 / Recombinant expression: No
MassTheoretical: 0.748065 kDa

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Component #50: ligand, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

LigandName: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / Number of Copies: 4 / Recombinant expression: No
MassTheoretical: 0.790145 kDa

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Component #51: ligand, CARDIOLIPIN

LigandName: CARDIOLIPIN / Number of Copies: 4 / Recombinant expression: No
MassTheoretical: 1.464043 kDa

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Component #52: ligand, ZINC ION

LigandName: ZINC ION / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.540905 MDa

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Component #53: ligand, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

LigandName: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.745421 kDa

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Component #54: ligand, S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)but...

LigandName: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.568704 kDa

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Component #55: ligand, 4'-PHOSPHOPANTETHEINE

LigandName: 4'-PHOSPHOPANTETHEINEPhosphopantetheine / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.358348 kDa

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Experimental details

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Sample preparation

SpecimenSpecimen state: particle / Method: cryo EM
Sample solutionSpecimen conc.: 5 mg/ml / pH: 7.4
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Temperature: 277 K / Humidity: 90 % / Details: 34s blotting

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 26 e/Å2 / Illumination mode: FLOOD BEAM
LensMagnification: 59000 X (nominal), 100720 X (calibrated) / Cs: 2.7 mm / Imaging mode: BRIGHT FIELD / Defocus: 500 - 3500 nm
Specimen HolderModel: FEI TITAN KRIOS AUTOGRID HOLDER
CameraDetector: FEI FALCON II (4k x 4k)

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Image acquisition

Image acquisitionNumber of digital images: 2600 / Sampling size: 14 microns

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 82000
3D reconstructionSoftware: RELION / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF

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Atomic model buiding

Modeling #1Refinement protocol: flexible / Refinement space: REAL
Input PDB model: 4HEA
Output model

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