SASDEM4: HrpG/HrpV/HrpJ gatekeeper complex from Erwinia amylovora

基本情報

• 登録情報: データベース: SASBDB / ID: SASDEM4

試料

HrpG/HrpV/HrpJ gatekeeper complex from Erwinia amylovora

• Type III secretion protein HrpGType three secretion system (protein), Erwinia amylovora (strain CFBP1430)
• Type III secretion protein HrpVType three secretion system (protein), Erwinia amylovora (strain CFBP1430)
• Hypersensitivity response secretion protein HrpJ (protein), Erwinia amylovora (strain CFBP1430)

機能・相同性

分子機能:

protein secretion by the type III secretion system / outer membrane / : / negative regulation of protein secretion / 細胞膜

ドメイン・相同性:

TyeA superfamily / Type III secretion system effector delivery regulator TyeA-related / Type III secretion regulator, YopN/LcrE/InvE/MxiC / Hypersensitivity response secretion-like, HrpJ / HrpJ-like domain

構成要素:

Hypersensitivity response secretion protein HrpJ / Type III secretion protein HrpG / Type III secretion protein HrpV

• 生物種: Erwinia amylovora (strain CFBP1430) (バクテリア)
• 引用: ジャーナル: mBio / 年: 2018; タイトル: Migration of Type III Secretion System Transcriptional Regulators Links Gene Expression to Secretion.; 著者: Spyridoula N Charova / Anastasia D Gazi / Efstratios Mylonas / Charalambos Pozidis / Blanca Sabarit / Dimitrios Anagnostou / Konstantina Psatha / Michalis Aivaliotis / Carmen R Beuzon / Nickolas J Panopoulos / Michael Kokkinidis / ; 要旨: Many plant-pathogenic bacteria of considerable economic importance rely on type III secretion systems (T3SSs) of the Hrc-Hrp 1 family to subvert their plant hosts. T3SS gene expression is regulated through the HrpG and HrpV proteins, while secretion is controlled by the gatekeeper HrpJ. A link between the two mechanisms was so far unknown. Here, we show that a mechanistic coupling exists between the expression and secretion cascades through the direct binding of the HrpG/HrpV heterodimer, acting as a T3SS chaperone, to HrpJ. The ternary complex is docked to the cytoplasmic side of the inner bacterial membrane and orchestrates intermediate substrate secretion, without affecting early substrate secretion. The anchoring of the ternary complex to the membranes potentially keeps HrpG/HrpV away from DNA. In their multiple roles as transcriptional regulators and gatekeeper chaperones, HrpV/HrpG provide along with HrpJ potentially attractive targets for antibacterial strategies. On the basis of scientific/economic importance, and are considered among the top 10 plant-pathogenic bacteria in molecular plant pathology. Both employ type III secretion systems (T3SSs) of the Hrc-Hrp 1 family to subvert their plant hosts. For Hrc-Hrp 1, no functional link was known between the key processes of T3SS gene expression and secretion. Here, we show that a mechanistic coupling exists between expression and secretion cascades, through formation of a ternary complex involving the T3SS proteins HrpG, HrpV, and HrpJ. Our results highlight the functional and structural properties of a hitherto-unknown complex which orchestrates intermediate T3SS substrate secretion and may lead to better pathogen control through novel targets for antibacterial strategies.

登録者

• Efstratios Mylonas (Institute of Molecular Biology and Biotechnology, FORTH)

モデル

• モデル #2378: ; タイプ: mix / ダミー原子の半径: 1.90 A; コメント: CORAL model with reconstructed polypetide chain.; カイ2乗値: 6.615184; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: HrpG/HrpV/HrpJ gatekeeper complex from Erwinia amylovora; 試料濃度: 1.5 mg/ml / Entity id: 1296 / 1297 / 1298
• バッファ: 名称: 20mM Tris-Cl, pH 8.0, 100mM NaCl, 2mM DTT, 0.5mM EDTA; pH: 8

要素 #1296

タイプ: protein
記述: Type III secretion protein HrpGType three secretion system
分子量: 19.166 / 由来: Erwinia amylovora (strain CFBP1430) / 参照: UniProt: D4HVP1
配列:

MSYYHHHHHH DYDIPTTENL YFQGAMRSTE LQQWAQRWLD DVGTDQQLTV DDGVVWLQRR ADRFFALAEL TINASLDDEL LGRALQLSAP TLRYFGRDAA ALAQQGNSLI LALAIRQLEV NAVCQQLESL LNQRDVWQTM LQQPRRKAAT HGAVPLHSLA FLPRGNHG

要素 #1297

タイプ: protein
記述: Type III secretion protein HrpVType three secretion system
分子量: 13.164 / 由来: Erwinia amylovora (strain CFBP1430) / 参照: UniProt: D4HVP4
配列:

MNNPCPQVTS LSALLSLLQL RQSCRWPVQQ GVELLALCGE RGRELMLNLQ PAFQPSGLYQ RLLSRRAQQL EIYDGCYLCL NHDRVLSCWR QLPESAMSDK QHIAQLFSLA GIHLAD

要素 #1298

タイプ: protein / 記述: Hypersensitivity response secretion protein HrpJ / 分子量: 42.4 / 由来: Erwinia amylovora (strain CFBP1430) / 参照: UniProt: D4HVM8
配列:

MKIAPVLPGI QPPHPVAINA ADEAPAPTSV APSAAASASA RMPVSPLEDS MEEVSMTFAE HVERKSKALN QRRVVAQPGG RAQQHIERIE KLDELLKLLE HPQHARLETQ NQKMQTLLQH SASPGMDELL HAAGGDPARC DVLLRHTLLQ AQRTQNSELA TKAAQSLELL HKEKGAEVSA GLNTASAIAS FSTDPTQKQV MRQLYYQTIV HLQSGNAMLD ALLNSFGSAH FNQGLRTLQR ALADDIASRS SSIPRRALQK ILSSLQDASN ISQTLTASKT LLTRLAGKLP TINFTSLELT RRLLNLSGNG AYLRDLQNLS RDVAGQHPHH QVLFLSGLVP LVYHLPLGLW RDDKSKNRQM ALNLLRKVIS DYAGQEPRAS LTQPRTAL

実験情報

• ビーム: 設備名称: SOLEIL SWING / 地域: Saint-Aubin / 国: France / 線源: X-ray synchrotronシンクロトロン / 波長: 0.1033 Å / スペクトロメータ・検出器間距離: 3.1 mm
• 検出器: 名称: AVIEX PCCD170170 / タイプ: CCD

スキャン

タイトル: HrpG/HrpV/HrpV gatekeeper complex from Erwinia amylovora
測定日: 2016年4月3日 / 保管温度: 4 °C / セル温度: 15 °C / 照射時間: 0.5 sec. / フレーム数: 30 / 単位: 1/A /

	Min	Max
Q	0.0216	0.4918

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.6 / ポイント数: 456 /

	Min	Max
Q	0.02158	0.2798
P(R) point	1	456
R	0	160

結果

D max: 16 / カーブのタイプ: single_conc /

	実験値	Standard	Porod
分子量	74.7 kDa	75 kDa	78 kDa

	P(R)	Guinier	Guinier error
前方散乱 I0	0.2855	0.272	-
慣性半径, Rg	4.36 nm	4 nm	0.2