+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDBW4 |
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試料 | Albumen gland precursor of ovorubin (lipoglycocarotenoprotein agPcOvo: an oligomeric protein)
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機能・相同性 | Perivitellin ovorubin-2 / Perivitellin ovorubin-1 / Perivitellin ovorubin-3 機能・相同性情報 |
生物種 | Pomacea canaliculata (スクミリンゴガイ) |
引用 | ジャーナル: Physiol Biochem Zool / 年: 2017 タイトル: Apple Snail Perivitellin Precursor Properties Help Explain Predators' Feeding Behavior. 著者: María Pilar Cadierno / Marcos Sebastián Dreon / Horacio Heras / 要旨: In contrast with vitellogenin maturation, it is unknown whether gastropod perivitellin precursors are subject to large structural changes. The gastropod reproductive tract includes an accessory ...In contrast with vitellogenin maturation, it is unknown whether gastropod perivitellin precursors are subject to large structural changes. The gastropod reproductive tract includes an accessory organ, the albumen gland (AG), that produces and secretes perivitelline fluid. In the apple snail Pomacea canaliculata, the large, reddish-pink AG provides eggs with perivitellins that are defensive against predators. Although the AG makes a considerable contribution to apple snail biomass, field observations indicate that it is rejected by avian and mammalian predators, although the underlying reason remains unknown. By analyzing the structure-function properties of P. canaliculata perivitellin precursors, we provide insight into perivitellin maturation and its relationship with apple snail predator feeding behavior. Structural analysis using small-angle X-ray scattering, absorption and fluorescence spectroscopy, circular dichroism, electrophoresis, chromatography, and partial proteolysis showed that the size, shape, and structure of perivitellin precursors resemble those of egg mature forms. Functional analysis indicates that the precursors of the defensive perivitellins ovorubin (PcOvo) and perivitellin-2 (PcPV2) are highly stable and antinutritive, withstanding proteinase digestion and displaying structural stability of their quaternary structure under a wide pH range (4.0-10.0). Furthermore, AG extracts limit a predator's ability to digest nutrients and are toxic to mice (median lethal concentration 96 h after administration: 5.9 mg/kg). Treated mice displayed neurologic signs similar to those produced by egg PcPV2. Results indicate that apple snails store active precursors of egg proteins inside the AG, providing evidence that gastropod perivitellin precursors do not experience the large structural processing of invertebrate vitellogenin maturation. These defensive proteins provide the apple snail AG with neurotoxic, antinutritive, and antidigestive activity, a likely explanation for the predators' feeding behavior. |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #537 | タイプ: dummy / ソフトウェア: (r3695) / ダミー原子の半径: 3.10 A / カイ2乗値: 2.692881 / P-value: 0.000024 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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モデル #538 | タイプ: dummy / ソフトウェア: (r3695) / ダミー原子の半径: 3.10 A / カイ2乗値: 2.640625 / P-value: 0.000024 Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: Albumen gland precursor of ovorubin (lipoglycocarotenoprotein agPcOvo: an oligomeric protein) 試料濃度: 1.04-4.16 / Entity id: 343 / 344 / 345 |
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バッファ | 名称: 20 mM Tris-HCl / 濃度: 20.00 mM / pH: 8.5 |
要素 #343 | 名称: Ovo1 / タイプ: protein / 記述: Perivitellin ovorubin-1 / 分子量: 22.384 / 由来: Pomacea canaliculata / 参照: UniProt: J7I2T6 配列: MFVATSLLLA IATLVVRASP HNYLIMDIEP PKSVSERDIL NLLSPLQVKH SFRVTGSTRL LIVIRLDAQS YEKLDEITVP GKVEVIPAVN MADTMERCGV SWPRVELTDD NVTLFESEST LTDVTKEQLK AMLIGYGEHM SGLLQAHRFE YYQAAGATPH RHFVFVNSVP ...配列: MFVATSLLLA IATLVVRASP HNYLIMDIEP PKSVSERDIL NLLSPLQVKH SFRVTGSTRL LIVIRLDAQS YEKLDEITVP GKVEVIPAVN MADTMERCGV SWPRVELTDD NVTLFESEST LTDVTKEQLK AMLIGYGEHM SGLLQAHRFE YYQAAGATPH RHFVFVNSVP DEIEVFGREG VDIWGGPGEF VVKPQYVTRI |
要素 #344 | 名称: Ovo2 / タイプ: protein / 記述: Perivitellin ovorubin-2 / 分子量: 23.801 / 由来: Pomacea canaliculata / 参照: UniProt: J7HZ90 配列: MAEHRIPVLL LLVVVAASSL AQQIPLQKHY IIYEVRNIEK TPEEVKEEMK DTDILYSFKA LGAPSYHIVV EVNPRNMRKL EEVELKGKIR MVPVVNMVDV AETLGVSWPR SGARLLDVNL TLIERTLNQE GLTSQESEAH LKGFMEELKD RLQQYNYQAF FTIGASPPKM ...配列: MAEHRIPVLL LLVVVAASSL AQQIPLQKHY IIYEVRNIEK TPEEVKEEMK DTDILYSFKA LGAPSYHIVV EVNPRNMRKL EEVELKGKIR MVPVVNMVDV AETLGVSWPR SGARLLDVNL TLIERTLNQE GLTSQESEAH LKGFMEELKD RLQQYNYQAF FTIGASPPKM YIYINIPYEE VDKFACIGIN QFGGPAAVNT TVSFISSFPK |
要素 #345 | 名称: Ovo3 / タイプ: protein / 記述: Perivitellin ovorubin-3 / 分子量: 34.957 / 由来: Pomacea canaliculata / 参照: UniProt: J7I5Z5 配列: MYALAIALLA FSTFVSNAFA NKEYLLLDIR DATTSEIISA LRDVEIELKV KAKGIARHLI VVKQNDANLQ KLGEIDIPGR SCSTPVEDLD NLMEDIGISW PRNELTSVNV TLFERTLDLK DKTMEQFWSE AKAYGQLVKP VLSSFTYRAF KANGAYPPKV YFFVNLPREN ...配列: MYALAIALLA FSTFVSNAFA NKEYLLLDIR DATTSEIISA LRDVEIELKV KAKGIARHLI VVKQNDANLQ KLGEIDIPGR SCSTPVEDLD NLMEDIGISW PRNELTSVNV TLFERTLDLK DKTMEQFWSE AKAYGQLVKP VLSSFTYRAF KANGAYPPKV YFFVNLPREN LNDASSKGID IFGGPGKART TVQYISVCVA VSHSTFSCRQ SVGDMKCMFQ QPPVPARSSG RPGRYDNQVR HGVRIAAPVM ENFPNYILLS KGTFPPKSRF RSNLWGLVNG SRRISRRGKP LLHNKHPNHY QMELKYTIAT H |
-実験情報
ビーム | 設備名称: Brazilian Synchrotron Light Laboratory SAXS2 Beamline 地域: Campinas / 国: Brazil / 線源: X-ray synchrotron | |||||||||||||||||||||||||||||||||
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検出器 | 名称: MAR 165 CCD | |||||||||||||||||||||||||||||||||
スキャン | タイトル: Albumen gland precursor of ovorubin / 測定日: 2014年6月10日 / 保管温度: 25 °C / セル温度: 25 °C / 照射時間: 300 sec. / フレーム数: 5 / 単位: 1/nm /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 4.5a / ポイント数: 219 /
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結果 | カーブのタイプ: single_conc コメント: agPcOvo is an oligomeric lipoglycocarotenoprotein isolated from the albumen gland of P. canaliculata that consists of different perivitellin ovorubin subunits (Ovo1-3) that form a ...コメント: agPcOvo is an oligomeric lipoglycocarotenoprotein isolated from the albumen gland of P. canaliculata that consists of different perivitellin ovorubin subunits (Ovo1-3) that form a complex with a molecular weight of 300 kDa.
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